CN102409089A - 检测dna样品中预定位点的突变的试剂盒、方法及应用 - Google Patents
检测dna样品中预定位点的突变的试剂盒、方法及应用 Download PDFInfo
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Abstract
Description
序列编号 | 引物序列 | 引物说明 |
SEQ ID NO:33 | TTTGTTCACACCCCC | 位点35delG的延伸引物 |
SEQ ID NO:34 | gggcCTTTGTCTGCAACACCC | 位点167delT的延伸引物 |
SEQ ID NO:35 | ctccGCAACACCCTGCAGCCAG | 位点176_191del16的延伸引物 |
SEQ ID NO:36 | gcTGAACTTCCTCTTCTTCTC | 位点299_300delAT的延伸引物 |
SEQ ID NO:37 | ggATCCTGCTATGGGCC | 位点235delC的延伸引物 |
SEQ ID NO:38 | ccctaGTGGACTGCTACATTGCC | 位点538C>T的延伸引物 |
SEQ ID NO:39 | ctgcAGTAGGTGAAGATTTTCTTCT | 位点547G>A的延伸引物 |
SEQ ID NO:40 | GTCATTTCGGGAGTTAGTA | 位点281C>T的延伸引物 |
SEQ ID NO:41 | CTTGTAAGTTCATTACCTGTATAATTC | 位点589G>A的延伸引物 |
SEQ ID NO:42 | GCAGTAGCAATTATCGTC | 位点919-2A>G的延伸引物 |
SEQ ID NO:43 | GCCTTTGGGATCAGC | 位点1174A>T的延伸引物 |
SEQ ID NO:44 | CACCACTGCTCTTTCCC | 位点1226G>A的延伸引物 |
SEQ ID NO:45 | ttCCACCACTGCTCTTTCCCCCA | 位点1229C>T的延伸引物 |
SEQ ID NO:46 | AAAACAAATTTCTAGGGATAAAATA | 位点IVS15+5G>A的延伸引物 |
SEQ ID NO:47 | CTCCACAGTCAAGCA | 位点1975G>C的延伸引物 |
SEQ ID NO:48 | AGAACCTTACCACCCGC | 位点2027T>A的延伸引物 |
SEQ ID NO:49 | CAGAGTATAGCATCAAGGACC | 位点2162C>T的延伸引物 |
SEQ ID NO:50 | TCTGTAGATAGAGTATAGCATCA | 位点2168A>G的延伸引物 |
SEQ ID NO:51 | CGTACACACCGCCCGTCAC | 位点1494C>T的延伸引物 |
SEQ ID NO:52 | ACTTACCATGTTACGACTTG | 位点1555A>G的延伸引物 |
试剂 | 体积/反应 |
水(HPLE级) | 1.53微升 |
SAP酶缓冲液 | 0.17微升 |
SAP酶 | 0.30微升 |
PCR扩增产物 | 5.0微升 |
总体积 | 7.0微升 |
序列编号 | 引物序列 | 引物说明 |
SEQ ID NO:37 | ggATCCTGCTATGGGCC | 位点235delC的延伸引物 |
SEQ ID NO:42 | GCAGTAGCAATTATCGTC | 位点919-2A>G的延伸引物 |
SEQ ID NO:51 | CGTACACACCGCCCGTCAC | 位点1494C>T的延伸引物 |
SEQ ID NO:52 | ACTTACCATGTTACGACTTG | 位点1555A>G的延伸引物 |
Claims (10)
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CN201110301714.2A CN102409089B (zh) | 2011-10-08 | 2011-10-08 | 检测dna样品中预定位点的突变的试剂盒、方法及应用 |
TW101136210A TW201315812A (zh) | 2011-10-08 | 2012-09-28 | 檢測dna樣品中預定位點的突變的試劑盒、方法及應用 |
PCT/CN2012/001382 WO2013049975A1 (zh) | 2011-10-08 | 2012-10-12 | 检测dna样品中预定位点的突变的试剂盒、方法及应用 |
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Cited By (19)
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WO2013049975A1 (zh) * | 2011-10-08 | 2013-04-11 | 深圳华大基因科技有限公司 | 检测dna样品中预定位点的突变的试剂盒、方法及应用 |
CN103352073A (zh) * | 2013-05-14 | 2013-10-16 | 向华 | 用于检测与遗传性耳聋相关的基因snp的引物***及其用途 |
CN104120133A (zh) * | 2013-04-28 | 2014-10-29 | 中国人民解放军总医院 | 基因突变体及其应用 |
CN104131008A (zh) * | 2014-07-24 | 2014-11-05 | 深圳华大基因医学有限公司 | Dna标签、pcr引物及其应用 |
CN104232631A (zh) * | 2014-08-26 | 2014-12-24 | 深圳华大基因医学有限公司 | 标签、标签引物、试剂盒及其用途 |
CN104498609A (zh) * | 2014-12-18 | 2015-04-08 | 亚能生物技术(深圳)有限公司 | 一种遗传性耳聋基因检测的核酸膜条及试剂盒 |
CN105087756A (zh) * | 2014-04-23 | 2015-11-25 | 北京贝瑞和康生物技术有限公司 | 一种无创检测胎儿耳聋致病基因突变的方法及试剂盒 |
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CN105296471A (zh) * | 2014-08-01 | 2016-02-03 | 天津华大基因科技有限公司 | Dna标签、pcr引物及其应用 |
CN105803052A (zh) * | 2014-12-31 | 2016-07-27 | 天津华大基因科技有限公司 | 检测乙醇耐受基因的方法和试剂盒 |
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CN106480222A (zh) * | 2016-12-20 | 2017-03-08 | 广州中心法则生物科技有限公司 | 基于悬浮微珠阵列***检测遗传性耳聋的探针、引物、检测试剂盒及检测方法 |
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CN112538524A (zh) * | 2019-12-31 | 2021-03-23 | 北京毅新博创生物科技有限公司 | 用于检测与耳聋相关的基因snp的检测产品 |
CN114250268A (zh) * | 2021-12-23 | 2022-03-29 | 北京毅新博创生物科技有限公司 | 检测mRNA样品的加帽效率的产品及用途 |
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CN106811533B (zh) * | 2017-03-06 | 2020-10-16 | 亚能生物技术(深圳)有限公司 | 一种遗传性耳聋基因检测试剂盒 |
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Cited By (26)
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