CN104846089B - 一种孕妇外周血中胎儿游离dna比例的定量方法 - Google Patents
一种孕妇外周血中胎儿游离dna比例的定量方法 Download PDFInfo
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原始reads数目 | 过滤后reads数目 | 有效reads比例 | |
样本1 | 3732137 | 3423150 | 91.7% |
样本2 | 3523141 | 3261449 | 92.6% |
样本3 | 3869887 | 3494950 | 90.3% |
样本4 | 6453416 | 6019736 | 93.2% |
质量过滤后reads数目 | 比对过滤后的reads数目 | 有效reads比例 | |
样本1 | 3423150 | 2385351 | 69.7% |
样本2 | 3261449 | 2295840 | 70.4% |
样本3 | 3494950 | 2150867 | 61.5% |
样本4 | 6019736 | 4113254 | 68.3% |
样本1 | 0.19% |
样本2 | 0.24% |
样本3 | 0.14% |
样本4 | 0.18% |
样本编号 | 胎儿游离DNA浓度 | 男胎Y计算的胎儿游离DNA浓度 |
样本1 | 9.3% | 10% |
样本2 | 10.3% | 10.5% |
样本3 | 8.7% | / |
样本4 | 13.2% | / |
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Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105926043B (zh) * | 2016-04-19 | 2018-08-28 | 苏州贝康医疗器械有限公司 | 一种提高孕妇血浆游离dna测序文库中胎儿游离dna占比的方法 |
CN106755486A (zh) * | 2017-01-19 | 2017-05-31 | 人和未来生物科技(长沙)有限公司 | 无创产前胎儿β 型地贫基因突变检测文库构建方法、检测方法和试剂盒 |
CN106755485A (zh) * | 2017-01-19 | 2017-05-31 | 人和未来生物科技(长沙)有限公司 | 无创产前胎儿α 型地贫基因突变检测文库构建方法、检测方法和试剂盒 |
CN107133491B (zh) * | 2017-03-08 | 2020-05-29 | 广州市达瑞生物技术股份有限公司 | 一种获取胎儿游离dna浓度的方法 |
CN108220451B (zh) * | 2017-12-08 | 2020-10-27 | 北京科迅生物技术有限公司 | 胎儿游离核酸浓度的检测方法及试剂盒 |
CN109979529B (zh) * | 2017-12-28 | 2021-01-08 | 北京安诺优达医学检验实验室有限公司 | Cnv检测装置 |
CN109461473B (zh) * | 2018-09-30 | 2019-12-17 | 北京优迅医疗器械有限公司 | 胎儿游离dna浓度获取方法和装置 |
CN110580934B (zh) * | 2019-07-19 | 2022-05-10 | 南方医科大学 | 一种基于外周血游离dna高通量测序的妊娠期相关疾病预测方法 |
CN110993024B (zh) * | 2019-12-20 | 2023-08-22 | 北京科迅生物技术有限公司 | 建立胎儿浓度校正模型的方法及装置与胎儿浓度定量的方法及装置 |
CN113584178A (zh) * | 2020-04-30 | 2021-11-02 | 深圳华大法医科技有限公司 | 一种无创亲子检测分析方法和装置 |
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GB2488358A (en) * | 2011-02-25 | 2012-08-29 | Univ Plymouth | Enrichment of foetal DNA in maternal plasma |
CN103215350B (zh) * | 2013-03-26 | 2016-11-02 | 苏州贝康医疗器械有限公司 | 一种基于单核苷酸多态性位点的孕妇血浆中胎儿dna含量的测定方法 |
CN104073554B (zh) * | 2014-04-28 | 2016-04-13 | 王弢 | 血浆特定片段游离dna定量检测试剂盒 |
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