CN102406231B - Fermented rose extract for cigarettes and preparation method thereof - Google Patents
Fermented rose extract for cigarettes and preparation method thereof Download PDFInfo
- Publication number
- CN102406231B CN102406231B CN2010102888283A CN201010288828A CN102406231B CN 102406231 B CN102406231 B CN 102406231B CN 2010102888283 A CN2010102888283 A CN 2010102888283A CN 201010288828 A CN201010288828 A CN 201010288828A CN 102406231 B CN102406231 B CN 102406231B
- Authority
- CN
- China
- Prior art keywords
- rose
- extract
- culture medium
- fermented
- cigarettes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000220317 Rosa Species 0.000 title claims abstract description 45
- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims abstract description 27
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 21
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 21
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 10
- 239000008103 glucose Substances 0.000 claims abstract description 10
- 235000015097 nutrients Nutrition 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 238000004321 preservation Methods 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 9
- 238000010992 reflux Methods 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000012982 microporous membrane Substances 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 5
- 241000208125 Nicotiana Species 0.000 abstract description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract description 7
- 239000003205 fragrance Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 235000009508 confectionery Nutrition 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 1
- 230000007794 irritation Effects 0.000 abstract 1
- 239000008132 rose water Substances 0.000 abstract 1
- 239000000779 smoke Substances 0.000 abstract 1
- 235000019605 sweet taste sensations Nutrition 0.000 abstract 1
- 235000013599 spices Nutrition 0.000 description 8
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000002304 perfume Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 241000628997 Flos Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 2
- 235000010254 Jasminum officinale Nutrition 0.000 description 2
- 240000005385 Jasminum sambac Species 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 239000003546 flue gas Substances 0.000 description 2
- 235000013355 food flavoring agent Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000006066 Rosa rugosa Species 0.000 description 1
- 235000000659 Rosa rugosa Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Landscapes
- Fats And Perfumes (AREA)
- Seasonings (AREA)
Abstract
The invention relates to a fermented rose extract for cigarettes and a preparation method thereof. The fermented rose extract for cigarettes is prepared by adding rose water extract into glucose to obtain a culture medium; preparing a culture solution from a culture of which the collection number is CCTCC No.M209272 and the collection name is saccharomyces cerevisiae HHL-1; and placing the culture medium into the culture solution for fermentation. The fermented rose extract for cigarettes has strong fragrance of rose and pleasant acid and sweet fragrance. When the fermented rose extract is added into cigarettes, the cigarettes offer rich tobacco fragrance, have enhanced sweet taste and have the effects of relieving irritation and softening smoke.
Description
Technical field
The invention belongs to the application of microbial fermentation technology, specifically a kind of fermented rose extract for cigarettes and preparation method thereof.
Technical background
The production of flavouring essence for tobacco, spices is mainly manually synthesize and extract from natural animal-plant in the world at present.Artificial synthetic flavouring essence for tobacco, spices are because people have arguement to its safety issue always, thereby its application is restricted.The trend gone back to nature along with the mankind, people increase day by day to the flavouring essence for tobacco of safety, the demand of spices, natural essence, the spices for cigarette extracted from animals and plants can meet the demand of this security, but cigarette is subject to the restriction of raw material sources with natural animal-plant spices.As produce 1kg Jasmine essential oil and need 5 * 10
6the kg Jasmine, thus the development and utilization of natural perfume material material is restricted.Biotechnology is the high-tech technology developed rapidly in recent years, and the approach that biotechnology is produced tobacco aromatics using comprises extraction from plant cell, culture plant cell, enzyme process is synthetic and microorganism is fermented etc.It is generally acknowledged, biological technique method is the forming process of simulation natural animal-plant spices, and the essence and flavoring agent that adopts biotechnology to produce can be equal to natural essence and flavoring agent.Therefore, by the fragrance material of biotechnology development and production high value, for a brand-new direction has been opened up in the production of natural perfume material.
Rose (Rosa rugosa Thunb) is rose family gul, is rare flower, have again higher view and admire, eat, medicinal and its cosmetic values, be widely used in the industries such as gardening, food, tobacco and daily use chemicals.Rose is the raw material of cigarette with Flos Rosae Rugosae extract, and prepared Flos Rosae Rugosae extract has the effect of enriching cigarette perfume (or spice) in cigarette by traditional refluxing extraction, but it also has the excitant of increasing, increases counter productive residual, that make flue gas become coarse.
The content of invention
Purpose of the present invention is exactly the defect for existing Flos Rosae Rugosae extract, and a kind of fermented rose extract for cigarettes and preparation method thereof is provided, and it has increased the category of tobacco aromatics using.
The technical solution used in the present invention is: it is to add glucose to make culture medium the rose wate extract, be that the culture that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomycescerevisiae HHL-1 makes nutrient solution by preserving number, then culture medium inserted in nutrient solution and fermented and be prepared from.Above-mentioned Saccharomyces Cerevisiae in S accharomyces cerevisiae HHL-1 on November 17th, 2009 at Wuhan, China, Wuhan University, preservation has been carried out at Chinese Typical Representative culture collection center.
The present invention's technical scheme preferably is: described rose wate extract be by rose and water by weight 1: the ratio refluxing extraction 1-3h of 5-10, the collected filtrate of 0.8 μ m filtering with microporous membrane; Described culture medium is to add glucose and the 0.05-0.75% sodium chloride that percentage by weight is 0.1%-5% to make culture medium in the rose wate extract; Described nutrient solution is to be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomyces cerevisiae HHL-1 and are inoculated in the LB culture medium by preserving number, makes its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution.
Preparation method of the present invention comprises the following steps:
(1), by rose and water in 1: the ratio refluxing extraction 1-3h of 5-10,0.8 μ m filtering with microporous membrane, collect filtrate, obtains the rose wate extract;
(2), to adding glucose and the 0.05-0.75% sodium chloride that percentage by weight is 0.1%-5% in the rose wate extract, make culture medium;
(3), by preserving number, be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomycescerevisiae HHL-1 and are inoculated in the LB culture medium, make its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution;
(4), culture medium is inserted in nutrient solution and fermented;
(5), the first zymotic fluid that obtains after fermentation is filtered under 0.8 μ m miillpore filter, get clarified solution, then filter under 0.2 μ m miillpore filter, obtain zymotic fluid;
(6), to adding the propane diols that percentage by weight is 2%-20% in zymotic fluid, obtain fermented rose extract for cigarettes.
Wherein zymotechnique is: the pH value is 4.0-8.0, and speed of agitator is 80-200rpm, and throughput is 1-4VVM, and fermentation temperature is 20 ℃-30 ℃, and fermentation time is 2d-4d.
The prepared fermented rose extract for cigarettes of the present invention has strong Rose Essentielle, and with pleasant sour-sweet fragrance.Add in cigarette, it has the effect of enriching cigarette perfume (or spice), increasing sweet sense, reduce excitant and soft flue gas.
the specific embodiment
Below in conjunction with embodiment, the present invention is described further:
Embodiment 1
(1), the ratio refluxing extraction 1h by rose and water in 1: 5,0.8 μ m filtering with microporous membrane, collect filtrate, obtains the rose wate extract;
(2), to adding glucose and 0.05% sodium chloride that percentage by weight is 0.1% in the rose wate extract, make culture medium;
(3), by preserving number, be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomycescerevisiae HHL-1 and are inoculated in the LB culture medium, make its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution; Under aseptic condition, inoculate 0.1% aroma former saccharomyces cerevisiae HHL-1 solution;
(4), culture medium is inserted in nutrient solution and fermented;
(5), the first zymotic fluid that obtains after fermentation is filtered under 0.8 μ m miillpore filter, get clarified solution, then filter under 0.2 μ m miillpore filter, obtain zymotic fluid;
(6), to adding the propane diols that percentage by weight is 5% in zymotic fluid, obtain fermented rose extract for cigarettes.
Wherein zymotechnique is: the pH value is 4.0, and speed of agitator is 80rpm, and throughput is 4VVM, and fermentation temperature is 20 ℃-30C, and fermentation time is 4d.
Embodiment 2
(1), the ratio refluxing extraction 3h by rose and water in 1: 10,0.8 μ m filtering with microporous membrane, collect filtrate, obtains the rose wate extract;
(2), to adding glucose and 0.75% sodium chloride that percentage by weight is 2% in the rose wate extract, make culture medium;
(3), by preserving number, be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomycescerevisiae HHL-1 and are inoculated in the LB culture medium, make its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution; Under aseptic condition, inoculate 0.01% aroma former saccharomyces cerevisiae HHL-1 solution;
(4), culture medium is inserted in nutrient solution and fermented;
(5), the first zymotic fluid that obtains after fermentation is filtered under 0.8 μ m miillpore filter, get clarified solution, then filter under 0.2 μ m miillpore filter, obtain zymotic fluid;
(6), to adding the propane diols that percentage by weight is 20% in zymotic fluid, obtain fermented rose extract for cigarettes.
Wherein zymotechnique is: the pH value is 6.0, and speed of agitator is 200rpm, and throughput is 4VVM, and fermentation temperature is 20 ℃-30 ℃, and fermentation time is 3d.
Embodiment 3
(1), the ratio refluxing extraction 2h by rose and water in 1: 8,0.8 μ m filtering with microporous membrane, collect filtrate, obtains the rose wate extract;
(2), to adding glucose and 0.3% sodium chloride that percentage by weight is 5% in the rose wate extract, make culture medium;
(3), by preserving number, be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomycescerevisiae HHL-1 and are inoculated in the LB culture medium, make its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution; Under aseptic condition, inoculate 0.01% aroma former saccharomyces cerevisiae HHL-1 solution;
(4), culture medium is inserted in nutrient solution and fermented;
(5), the first zymotic fluid that obtains after fermentation is filtered under 0.8 μ m miillpore filter, get clarified solution, then filter under 0.2 μ m miillpore filter, obtain zymotic fluid;
(6), to adding the propane diols that percentage by weight is 10% in zymotic fluid, obtain fermented rose extract for cigarettes.
Wherein zymotechnique is: the pH value is 8.0, and speed of agitator is 100rpm, and throughput is 4VVM, and fermentation temperature is 20 ℃-30 ℃, and fermentation time is 3d.
Claims (4)
1. a fermented rose extract for cigarettes, it is to add glucose to make culture medium the rose wate extract, by preserving number, be CCTCC NO:M209272, the culture that the preservation name is called Saccharomyces Cerevisiae in S accharomyces cerevisiae HHL-1 makes nutrient solution, then culture medium is inserted in nutrient solution and fermented and be prepared from, wherein said rose wate extract be by rose and water by weight 1: the ratio refluxing extraction 1-3h of 5-10, 0.81 the filtrate that μ m filtering with microporous membrane is collected, described culture medium is to add glucose and the 0.05-0.75% sodium chloride that percentage by weight is 0.1%-5% to make culture medium in the rose wate extract.
2. a kind of fermented rose extract for cigarettes according to claim 1, wherein said nutrient solution is to be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomyces cerevisiae HHL-1 and are inoculated in the LB culture medium by preserving number, makes its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution.
3. the preparation method of a fermented rose extract for cigarettes, it comprises the following steps:
(1), by rose and water in 1: the ratio refluxing extraction 1-3h of 5-10,0.8 μ m filtering with microporous membrane, collect filtrate, obtains the rose wate extract;
(2), to adding glucose and the 0.05-0.75% sodium chloride that percentage by weight is 0.1%-5% in the rose wate extract, make culture medium;
(3), by preserving number, be that CCTCC NO:M209272, preservation name are called Saccharomyces Cerevisiae in S accharomyces cerevisiae HHL-1 and are inoculated in the LB culture medium, make its bacteria concentration reach 1.5 * 10
10individual/ml, make aroma former saccharomyces cerevisiae nutrient solution;
(4), culture medium is inserted in nutrient solution and fermented;
(5), the first zymotic fluid that obtains after fermentation is filtered under 0.8 μ m miillpore filter, get clarified solution, then filter under 0.2 μ m miillpore filter, obtain zymotic fluid;
(6), to adding the propane diols that percentage by weight is 2%-20% in zymotic fluid, obtain fermented rose extract for cigarettes.
4. the preparation method of a kind of fermented rose extract for cigarettes according to claim 3, wherein zymotechnique is: the pH value is 4.0-8.0, and speed of agitator is 80-200rpm, and throughput is 1-4VVM, fermentation temperature is 20 ℃-30 ℃, and fermentation time is 2d-4d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102888283A CN102406231B (en) | 2010-09-21 | 2010-09-21 | Fermented rose extract for cigarettes and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102888283A CN102406231B (en) | 2010-09-21 | 2010-09-21 | Fermented rose extract for cigarettes and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102406231A CN102406231A (en) | 2012-04-11 |
| CN102406231B true CN102406231B (en) | 2013-12-11 |
Family
ID=45908798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102888283A Expired - Fee Related CN102406231B (en) | 2010-09-21 | 2010-09-21 | Fermented rose extract for cigarettes and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102406231B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484249B (en) * | 2013-07-30 | 2015-01-21 | 湖北中烟工业有限责任公司 | Pear-fermented tobacco flavor and preparation method thereof |
| CN104223352B (en) * | 2014-08-19 | 2016-02-24 | 云南中烟工业有限责任公司 | A kind of Radix Glycyrrhizae fermentation gained tobacco aromatics using and application thereof |
| CN106136297A (en) * | 2016-08-24 | 2016-11-23 | 陈佐坚 | A kind of sophora flower wine odor type pipe tobacco |
| CN106957862A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of immobilized yeast prepares method of the cigarette with flores aurantii extract |
| CN106957839A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract |
| CN107674885A (en) * | 2016-10-13 | 2018-02-09 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares cigarette feltwort extract |
| CN106957861A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares cigarette violet extract |
| CN106434829B (en) * | 2016-12-05 | 2019-09-10 | 湖北中烟工业有限责任公司 | Fermented rose extract for cigarettes and preparation method thereof |
| EP3744401B1 (en) * | 2019-05-28 | 2023-06-14 | Chanel Parfums Beauté | Method for extracting plants comprising a step of fermentation with saccharomyces cerevisiae |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106959A (en) * | 1985-09-19 | 1987-05-20 | 贝特莱夫国际公司 | Herbal chewing tobacco and snuff composition |
| CN1745667A (en) * | 2005-08-16 | 2006-03-15 | 段鸿昌 | Production of cigarettes with rose as raw materials |
| WO2008121403A1 (en) * | 2007-03-30 | 2008-10-09 | Gp Technologies, Inc. | Porous plastic smokeless tobacco substitutes |
| CN101497844A (en) * | 2008-01-30 | 2009-08-05 | 湖北中烟工业有限责任公司 | Method for preparing cigarette flavor by microbial fermentation of coffee |
| CN101548797A (en) * | 2008-03-31 | 2009-10-07 | 湖北中烟工业有限责任公司 | Cigarette and preparation method thereof |
-
2010
- 2010-09-21 CN CN2010102888283A patent/CN102406231B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106959A (en) * | 1985-09-19 | 1987-05-20 | 贝特莱夫国际公司 | Herbal chewing tobacco and snuff composition |
| CN1745667A (en) * | 2005-08-16 | 2006-03-15 | 段鸿昌 | Production of cigarettes with rose as raw materials |
| WO2008121403A1 (en) * | 2007-03-30 | 2008-10-09 | Gp Technologies, Inc. | Porous plastic smokeless tobacco substitutes |
| CN101497844A (en) * | 2008-01-30 | 2009-08-05 | 湖北中烟工业有限责任公司 | Method for preparing cigarette flavor by microbial fermentation of coffee |
| CN101548797A (en) * | 2008-03-31 | 2009-10-07 | 湖北中烟工业有限责任公司 | Cigarette and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102406231A (en) | 2012-04-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102406231B (en) | Fermented rose extract for cigarettes and preparation method thereof | |
| CN102178345B (en) | Method for preparing fermentation malt extract for cigarettes | |
| CN102226129A (en) | Preparation method of composite extract for fermentation type tobacco | |
| CN102187920A (en) | Method for preparing gold-flower Puer tea by inoculating fungus microorganisms | |
| CN102217780A (en) | Concrete flavouring for tobacco and preparation method and application thereof | |
| CN102952833A (en) | Agrocybe cylindracea polysaccharide and application thereof | |
| CN105062677A (en) | Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum | |
| CN106551422A (en) | Preparation method and application of the cigarette with Semen Ziziphi Spinosae extractum | |
| CN106434828A (en) | Preparation method of fermented parochetus communis perfumes for cigarettes | |
| CN109090697B (en) | Method for fermenting tobacco by mixed bacteria | |
| CN103451108B (en) | Aureobasidium pullulans OF-01 strain and method for preparing tobacco endogenous derived spice by using strain | |
| CN102628026A (en) | Bacillus methylotrophicus and preparation method and application of tobacco coffee extract thereof | |
| CN105713847B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing sanchi flower tobacco aromaticss with it | |
| CN108142988B (en) | Preparation method of momordica grosvenori extract for increasing aroma and keeping moisture | |
| CN102154370B (en) | Method for preparing flavor for elsholtzia kachinensis prain tobacco through multi-strain composite fermentation | |
| CN102987545B (en) | Preparation method of raspberry fermenting substance for cigarette | |
| CN105733962B (en) | A kind of trichoderma aureoviride HLMM-04 bacterial strain and the method for preparing tobacco aromaticss | |
| CN104974849B (en) | A kind of preparation method of fermentation type tobacco garden beet medicinal extract | |
| CN106434829B (en) | Fermented rose extract for cigarettes and preparation method thereof | |
| CN106957866A (en) | A kind of immobilized yeast prepares method of the cigarette with red bayberry extract | |
| CN102392050B (en) | Endophyte bio-transformed raisin extract and preparation and application in flavoring thereof | |
| CN102258213B (en) | Complex enzyme bacterium preparation used for tobacco thread production and preparation method thereof | |
| CN109380762A (en) | A kind of combined extraction method and the application of passion fruit pericarp volatile oil and polysaccharide | |
| CN108251216A (en) | A kind of preparation method and application of the Pu'er tea absolute oil containing the fragrant component of Viscμm articulatum Burm f cause | |
| CN105733961B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing radix scrophulariae tobacco aromaticss with it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131211 Termination date: 20140921 |
|
| EXPY | Termination of patent right or utility model |