CN102398992A - Ammonia-oxidizing bacteria immobilization method employing composite carrier - Google Patents
Ammonia-oxidizing bacteria immobilization method employing composite carrier Download PDFInfo
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- CN102398992A CN102398992A CN2010102753341A CN201010275334A CN102398992A CN 102398992 A CN102398992 A CN 102398992A CN 2010102753341 A CN2010102753341 A CN 2010102753341A CN 201010275334 A CN201010275334 A CN 201010275334A CN 102398992 A CN102398992 A CN 102398992A
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- oxidizing bacteria
- ammonia
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- immobilization method
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Abstract
The invention provides an ammonia-oxidizing bacteria immobilization method. Immobilized ammonia-oxidizing bacteria are placed into a nitration section in a sewage processing process. With the immobilized ammonia-oxidizing bacteria, denitrification starting speed and ammonia nitrogen removing efficiency can be greatly improved. With the method provided by the invention, a good technical guarantee is provided for denitrification of sewage with high COD concentration. According to the invention, immobilizing particles with a composite material and a fibrous material are adopted. The particles provide higher mechanical strength and mass transfer properties than common immobilized microbes.
Description
Technical field:
The present invention relates to a kind of ammonia nitrogen oxidized form microbial immobilization technology, belong to microbial fermentation and water treatment field.
Background technology:
In the conventional WWT, denitrogenation is important link, at AO, and A
2In the water technologies such as O, ammonia nitrogen normal open peroxide turns to nitric acid, is reduced to nitrogen after nitrification liquid refluxes and removes.In this reaction process, the most key conditioning step is the oxidation of ammonia nitrogen.The oxidation of ammonia nitrogen needs three conditions, and one is sufficient dissolved oxygen, and the 2nd, lower COD concentration, the 3rd, a large amount of ammonia oxidizing bacteria (nitrobacteria).Nitrobacteria has two types, and one type is ammonia oxidizing bacteria, and another kind of is nitrite-oxidizing bacteria.As far as denitrifying microorganism, the product nitrite of ammonia oxidizing bacteria just can be used as the denitrogenation substrate, so ammonia nitrogen is oxidizing to this stage denitrification that can reflux.
For a reactor drum reasonable in design, create suitable dissolved oxygen and realize that under higher COD concentration the important link of nitration reaction possesses enough concentration and nitrobacteria efficiently exactly.And microbial immobilized technology is the direct method of possessing enough concentration nitrobacterias.Microbial immobilized technology is exactly to adopt suitable carriers that mikrobe purebred or that separate to confirm is fixed on certain space, makes it not diluted with current, takes away, and can guarantee in the reactor drum biocatalytic reaction efficiently like this.
The fixation support that is suitable for wastewater treatment should have following performance: 1) nontoxic to mikrobe; 2) mass-transfer performance is good; 3) stable in properties; 4) cost simple for production is low.
Summary of the invention:
The present invention has developed and has a kind ofly utilized complex carrier to be fixed as the product of microcapsule ammonia oxidizing bacteria.The bacterium of this product embedding is the ammonia oxidizing bacteria of purebred cultivation.Strain separating is from the nitrated segment process part of sewage work.Fixation support is that PVA and gac are main mixture.
Microbial cultivation process:, be in charge of shake-flask seed, seeding tank, fermentor tank through slant tube.This process is in this special introduction, and the professional of can generally being fermented understands this process.
The mikrobe fixing means: 1. the ammonia oxidizing bacteria that fermentation is spread cultivation out is centrifugal, centrifugal speed 4000～10000g.Bacterium mud is collected the back and is adopted the above activity charcoal powder mixing and absorption of 1000 orders, activity charcoal powder and bacterium mud volume ratio 100: 1～1: 1, and the mixture after stirring is for use 4 ℃ of preservations.2. PVA is prepared as 10% the aqueous solution, and with bacterium mud mixture, CMC and this aqueous solution thorough mixing.This mixture is splashed in the saturated BAS, place more than the 10h, it is fully reacted.3. leach immobilization particle, clean, be placed on 4 ℃ of preservations with saline water.
Need adopt the grid thing to isolate immobilization particle during use, in order to avoid taken out of by water, and place reactor drum air water stream to impact less space as far as possible this immobilization particle as far as possible.
Claims (2)
1. method that adopts matrix material fixed ammonia oxidizing bacteria is characterized in that:
The used matrix material of embedding has comprised two kinds of inorganic materials and a kind of high molecular polymer.Inorganic materials is gac and CMC, and organic materials is PVA.
The used ammonia oxidizing bacteria of embedding is the bacterium of purebred cultivation, and microbial culture concentration is 107～108cpu.
2. matrix material according to claim 1, the ratio of gac and CMC are 1: 1～1: 50, and the ratio of gac and bacterium mud is 1: 1～1: 100.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102753341A CN102398992A (en) | 2010-09-08 | 2010-09-08 | Ammonia-oxidizing bacteria immobilization method employing composite carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010102753341A CN102398992A (en) | 2010-09-08 | 2010-09-08 | Ammonia-oxidizing bacteria immobilization method employing composite carrier |
Publications (1)
| Publication Number | Publication Date |
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| CN102398992A true CN102398992A (en) | 2012-04-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010102753341A Pending CN102398992A (en) | 2010-09-08 | 2010-09-08 | Ammonia-oxidizing bacteria immobilization method employing composite carrier |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004228A (en) * | 2017-12-28 | 2018-05-08 | 光合强化（北京）生物科技有限公司 | Acrylic resin composite plate of fixed ammonia oxidizing bacteria and preparation method thereof |
| CN111573831A (en) * | 2020-04-17 | 2020-08-25 | 北京工业大学 | Preparation method of denitrifying embedded bacteria particles for sewage treatment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199712A (en) * | 1997-02-28 | 1998-11-25 | 可乐丽股份有限公司 | Waste water treatment apparatus |
| CA2468780A1 (en) * | 2001-12-03 | 2003-06-12 | C.R. Bard, Inc. | Microbe-resistant medical device, microbe-resistant polymeric coating and methods for producing same |
| CN1482080A (en) * | 2002-06-24 | 2004-03-17 | 可乐丽股份有限公司 | Apparatus and method for treating wastewater containing nitrogen-containing dyes |
| CN1928081A (en) * | 2006-09-28 | 2007-03-14 | 武汉益生泉生物科技开发有限责任公司 | Artificial biological film and preparation method |
| CA2750310A1 (en) * | 2009-01-26 | 2010-07-29 | Indicator Systems International, Inc. | Indicators for detecting the presence of metabolic byproducts from microorganisms |
-
2010
- 2010-09-08 CN CN2010102753341A patent/CN102398992A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199712A (en) * | 1997-02-28 | 1998-11-25 | 可乐丽股份有限公司 | Waste water treatment apparatus |
| CA2468780A1 (en) * | 2001-12-03 | 2003-06-12 | C.R. Bard, Inc. | Microbe-resistant medical device, microbe-resistant polymeric coating and methods for producing same |
| CN1482080A (en) * | 2002-06-24 | 2004-03-17 | 可乐丽股份有限公司 | Apparatus and method for treating wastewater containing nitrogen-containing dyes |
| CN1928081A (en) * | 2006-09-28 | 2007-03-14 | 武汉益生泉生物科技开发有限责任公司 | Artificial biological film and preparation method |
| CA2750310A1 (en) * | 2009-01-26 | 2010-07-29 | Indicator Systems International, Inc. | Indicators for detecting the presence of metabolic byproducts from microorganisms |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004228A (en) * | 2017-12-28 | 2018-05-08 | 光合强化（北京）生物科技有限公司 | Acrylic resin composite plate of fixed ammonia oxidizing bacteria and preparation method thereof |
| CN111573831A (en) * | 2020-04-17 | 2020-08-25 | 北京工业大学 | Preparation method of denitrifying embedded bacteria particles for sewage treatment |
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Application publication date: 20120404 |