CN102398992A - 一种复合载体固定化氨氧化细菌的方法 - Google Patents

一种复合载体固定化氨氧化细菌的方法 Download PDF

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CN102398992A
CN102398992A CN2010102753341A CN201010275334A CN102398992A CN 102398992 A CN102398992 A CN 102398992A CN 2010102753341 A CN2010102753341 A CN 2010102753341A CN 201010275334 A CN201010275334 A CN 201010275334A CN 102398992 A CN102398992 A CN 102398992A
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oxidizing bacteria
ammonia
immobilized
immobilization method
method employing
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杨昊
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JILIN ANNENG TECHNOLOGY INDUSTRY CO LTD
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JILIN ANNENG TECHNOLOGY INDUSTRY CO LTD
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Abstract

本发明提出了一种固定氨氧化细菌的方法，固定后的氨氧化细菌投放到污水处理的硝化段，能极大地提高脱氮启动速度和氨氮去除效率。为高COD浓度的废水的脱氮处理提供了良好的技术保障。采用复合材料和纤维状材料的固定化颗粒具有比照普通固定化微生物更高的机械强度和传质性能。

Description

一种复合载体固定化氨氧化细菌的方法
技术领域：
本发明涉及一种氨氮氧化型微生物的固定化技术，属于微生物发酵和水处理领域。
背景技术：
常规的污水处理中，脱氮是重要的环节，在AO，A2O等水处理工艺中，氨氮常通过氧化为硝酸，硝化液回流后还原为氮气去除。在这个反应过程中，最为关键的限制步骤是氨氮的氧化。氨氮的氧化需要三个条件，一个是充足的溶解氧，二是较低的COD浓度，三是大量的氨氧化细菌(硝化细菌)。硝化细菌有两类，一类是氨氧化细菌，另一类是亚硝酸氧化细菌。对反硝化微生物而言，氨氧化细菌的产物亚硝酸盐就可以作为脱氮底物，所以将氨氮氧化至这一阶段即可回流反硝化。
对于一个设计合理的反应器而言，创造合适的溶解氧和在较高的COD浓度下实现硝化反应重要的环节就是保有足够浓度和高效的硝化细菌。而微生物固定化技术是保有足够浓度硝化细菌的最直接方法。微生物固定化技术就是采用合适的载体将纯种或分离确定的微生物固定在一定的空间，使之不随水流被稀释，带走，这样可以保证反应器内高效的生物催化反应。
适合于废水处理的固定化载体应具有以下性能：1)对微生物无毒；2)传质性能好；3)性质稳定；4)制作简便成本低。
发明内容：
本发明开发了一种将氨氧化细菌利用复合载体固定为微胶囊的产品。该产品包埋的细菌为纯种培养的氨氧化细菌。菌种分离自污水处理厂硝化段工艺部分。固定化载体为PVA和活性炭为主的混合物。
微生物培养过程：经过斜面试管，分管，摇瓶种子，种子罐，发酵罐。此过程不在此专门介绍，这一过程可被一般发酵专业人员了解。
微生物固定方法：1.将发酵扩培出来的氨氧化细菌离心，离心速度4000～10000g。菌泥收集后采用1000目以上活性炭粉混合吸附，活性炭粉与菌泥体积比100∶1～1∶1，搅拌均匀后的混合物在4℃保存待用。2.将PVA制备为10％的水溶液，并将菌泥混合物、CMC与该水溶液充分混合。将该混合物滴入饱和硼酸溶液中，放置10h以上，使其充分反应。3.滤出固定化颗粒，用生理盐水洗净，放置在4℃保存。
使用时需要将固定化颗粒采用网格物隔离，以免被水带出，并尽量将该固定化颗粒尽可能置于反应器气水流冲击较小的空间。

Claims (2)

1.一种采用复合材料固定氨氧化细菌的方法，其特征在于：
包埋所用的复合材料包括了两种无机材料和一种高分子聚合物。无机材料为活性炭和CMC，有机材料为PVA。
包埋所用的氨氧化细菌为纯种培养的细菌，细菌培养浓度为107～108cpu。
2.根据权利要求1所述的复合材料，活性炭和CMC的比例为1∶1～1∶50，活性炭与菌泥的比例为1∶1～1∶100。
CN2010102753341A 2010-09-08 2010-09-08 一种复合载体固定化氨氧化细菌的方法 Pending CN102398992A (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108004228A (zh) * 2017-12-28 2018-05-08 光合强化（北京）生物科技有限公司 固定氨氧化细菌的聚丙烯树脂复合板及其制备方法
CN111573831A (zh) * 2020-04-17 2020-08-25 北京工业大学 一种用于污水处理的反硝化包埋菌颗粒制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1199712A (zh) * 1997-02-28 1998-11-25 可乐丽股份有限公司 废水处理装置
CA2468780A1 (en) * 2001-12-03 2003-06-12 C.R. Bard, Inc. Microbe-resistant medical device, microbe-resistant polymeric coating and methods for producing same
CN1482080A (zh) * 2002-06-24 2004-03-17 可乐丽股份有限公司 含有含氮染料的排水的处理装置和处理方法
CN1928081A (zh) * 2006-09-28 2007-03-14 武汉益生泉生物科技开发有限责任公司 一种人造生物膜及制备方法
CA2750310A1 (en) * 2009-01-26 2010-07-29 Indicator Systems International, Inc. Indicators for detecting the presence of metabolic byproducts from microorganisms

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1199712A (zh) * 1997-02-28 1998-11-25 可乐丽股份有限公司 废水处理装置
CA2468780A1 (en) * 2001-12-03 2003-06-12 C.R. Bard, Inc. Microbe-resistant medical device, microbe-resistant polymeric coating and methods for producing same
CN1482080A (zh) * 2002-06-24 2004-03-17 可乐丽股份有限公司 含有含氮染料的排水的处理装置和处理方法
CN1928081A (zh) * 2006-09-28 2007-03-14 武汉益生泉生物科技开发有限责任公司 一种人造生物膜及制备方法
CA2750310A1 (en) * 2009-01-26 2010-07-29 Indicator Systems International, Inc. Indicators for detecting the presence of metabolic byproducts from microorganisms

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108004228A (zh) * 2017-12-28 2018-05-08 光合强化（北京）生物科技有限公司 固定氨氧化细菌的聚丙烯树脂复合板及其制备方法
CN111573831A (zh) * 2020-04-17 2020-08-25 北京工业大学 一种用于污水处理的反硝化包埋菌颗粒制备方法

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Application publication date: 20120404