CN108004228A - Acrylic resin composite plate of fixed ammonia oxidizing bacteria and preparation method thereof - Google Patents
Acrylic resin composite plate of fixed ammonia oxidizing bacteria and preparation method thereof Download PDFInfo
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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Abstract
The invention discloses the acrylic resin composite plate of fixed ammonia oxidizing bacteria.The mixed liquor prepared after the cleaning of acrylic resin plate through potassium stannate, 2,5 dihydroxy benzenes sulfonic acid potassium, trifluoroacetic acid potassium, potassium hydrogen tartrate modified obtains substance B；The modified acrylic resin composite plate for obtaining substance C, fixing after ammonia oxidizing bacteria that obtained material is fixed ammonia oxidizing bacteria on substance C of mixed liquor that substance B is prepared through potassium tetroxalate dihydrate, agarose and potassium sorbate.The invention has the advantages that the acrylic resin composite plate of obtained fixed ammonia oxidizing bacteria has the function of ammonia nitrogen in strain activity height, energy efficient degradation waste water.
Description
Technical field
Functional material research and development and preparation field the invention belongs to environmental pollution treatment technology, more particularly to a kind of fixed ammonia
Acrylic resin composite plate of oxidizing bacteria and preparation method thereof.
Background technology
Immobilized microorganism technique is the biological skill to be grown up by the enzyme immobilization technology in biochemical industry in the sixties
Art, is fixed into particle by self-coagulation by free microorganism cell using method physically or chemically or is fixed on specific bed course
In material, it is kept activity, can recycle.In the seventies later stage, as the worsening of water pollution, the technology because
The advantages of it is unique and be applied to the biological treatment of industrial wastewater, compared with traditional suspended biological facture, immobilization is micro-
Biotechnology, which has, to be improved microbial cell concentration in reactor, keeps high-efficiency strain, microbe density high and be lost in less, reaction
The advantages that speed is fast, resistance to murder by poisoning ability is strong, processing equipment is simple, thus in recent years be always domestic and foreign scholars research hot spot it
One, while also achieve encouraging achievement in research.Immobilized microorganism technique can put into practicality it is critical that
The selection of fixation support material, should have Resistance to microbes, high mechanical strength, biography applied to microbial immobilized carrier
The characteristics such as matter performance is good, nontoxic to microorganism, property is stable, cheap, long lifespan.Currently used fixation support material
Material mainly has：Organic polymer carrier, inorganic carrier and complex carrier three major types.But still need and carrier is modified,
To improve the activity of carrier fixation of microbe and performance.
The Transport And Transformation of ammonia nitrogen is also required to the oxygen in consumption water body, and complete oxidation 1mg ammonia nitrogens probably need for nitrate nitrogen
4.6mg dissolved oxygens, the ammonia nitrogen of high-content, which enters the slower surface water body of renewal, can cause eutrophication, and algae is in matrix abundance
Under the conditions of raised growth breed, in the process can luxus consumption oxygen in water so that producing large amount of organic causes water body
It is black it is smelly seriously affect water quality, and the aquatiles such as fish can be caused dead because lacking oxygen.Ammonia and nitrogen pollution in water body environment
Requirement of the severe situation to the treatment effect of waste water treatment process, economic benefit etc. it is also higher and higher.Each sewage disposal
Processing of the factory to sanitary sewage and municipal wastewater generally selects traditional activated sludge process to carry out biological denitrificaion, due to environmental improvement
Cost increase, immobilized microorganism technique pass through continuous research and development, it will be in wastewater treatment or even water pollution control
In play an increasingly important role, still, also lack the material for fixing ammonia oxidizing bacteria at present, therefore, exploitation is used for
The material of fixed ammonia oxidizing bacteria has great importance for administering and repairing polluted by nitrogen water body.
The content of the invention
The object of the present invention is to provide a kind of acrylic resin composite plate of fixed ammonia oxidizing bacteria, the tool of its preparation method
Body step is as follows：
(1) the acrylic resin plate that length, width and thickness are respectively 10cm, 5cm and 0.5cm is added to 250mL matter
Measure in the sulfuric acid solution that percent concentration is 15%, stirred 5 minutes under the conditions of 1000r/min, be filtered to remove liquid, pass through
Placed 120 minutes in the drying box that temperature is 65 DEG C after the washing of 800mL deionized waters, obtain substance A；
(2) 6.3 grams of potassium stannates are added in 750mL deionized waters, stir 3 minutes, shake up under the conditions of 1000r/min
After be divided into 5 parts of equivalent, obtain mixed liquor M1, mixed liquor M2, mixed liquor M3, mixed liquor M4, mixed liquor M5；
(3) 4.4 grams of 2,5- dihydroxy benzenes sulfonic acids potassium and 5.7 grams of trifluoroacetic acid potassium are added in 250mL deionized waters,
Stirred 3 minutes under the conditions of 1000r/min, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor N1, mixed liquor N2, mixed liquor N3, mixed
Close liquid N4, mixed liquor N5；
(4) mixed liquor M1 and 1.9 grams of potassium hydrogen tartrates are added in mixed liquor N1,3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor O1；
(5) substance A is added in mixed liquor O1, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Body obtains substance A 1, and substance A 1 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 2；
(6) mixed liquor M2 and 1.7 grams of potassium hydrogen tartrates are added in mixed liquor N2,3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor O2；
(7) substance A 2 is added in mixed liquor O2, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Body obtains substance A 3, and substance A 3 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 4；
(8) mixed liquor M3 and 1.5 grams of potassium hydrogen tartrates are added in mixed liquor N3,3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor O3；
(9) substance A 4 is added in mixed liquor O3, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Body obtains substance A 5, and substance A 5 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 6；
(10) mixed liquor M4 and 1.3 grams of potassium hydrogen tartrates are added in mixed liquor N4, are stirred under the conditions of 1000r/min
3 minutes, obtain mixed liquor O4；
(11) substance A 6 is added in mixed liquor O4, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance A 7, and substance A 7 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 8；
(12) mixed liquor M5 and 1.1 grams of potassium hydrogen tartrates are added in mixed liquor N5, are stirred under the conditions of 1000r/min
3 minutes, obtain mixed liquor O5；
(13) substance A 8 is added in mixed liquor O5, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance A 9, and substance A 9 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B；
(14) 8.19 grams of potassium tetroxalate dihydrates are added in 1500mL deionized waters under 1000r/min stirring conditions,
Stir 3 minutes under the conditions of 1000r/min, then add 3.3 grams of potassium methoxides, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor P1,
Mixed liquor P2, mixed liquor P3, mixed liquor P4, mixed liquor P5；
(15) 8.8 grams of agaroses and 6.4 Keshan potassium sorbates are added in mixed liquor P1, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q1；
(16) substance B is added in mixed liquor Q1, is shaken 25 minutes in the shaking table that temperature is 38 DEG C, be filtered to remove liquid
Body obtains substance B 1, and substance B 1 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 2；
(17) 8.2 grams of agaroses and 6.1 Keshan potassium sorbates are added in mixed liquor P2, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q2；
(18) substance B 2 is added in mixed liquor Q2, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 3, and substance B 3 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 4；
(19) 7.6 grams of agaroses and 5.8 Keshan potassium sorbates are added in mixed liquor P3, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q3；
(20) substance B 4 is added in mixed liquor Q3, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 5, and substance B 5 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 6；
(21) 7.0 grams of agaroses and 5.5 Keshan potassium sorbates are added in mixed liquor P4, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q4；
(22) substance B 6 is added in mixed liquor Q4, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 7, and substance B 7 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 8；
(23) 6.4 grams of agaroses and 5.2 Keshan potassium sorbates are added in mixed liquor P5, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q5；
(24) substance B 8 is added in mixed liquor Q5, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 9, and substance B 9 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance C；
(25) by 1.8 grams of NH4NO3, 10.2 grams of NaCl, 2.3 grams of KH2PO4, 0.5 gram of FeCl3, 0.3 gram of CoCl2, 0.2 gram
ZnCl2, 3.3 grams of MgSO4, 1.6 grams of CaCl2With 0.1 gram of NiCl2It is added in 1000mL deionized waters, in 1000r/min conditions
Lower stirring 3 minutes, obtains mixed liquor R；
(26) it is 5 × 10 by 10mL bacteria containing amounts12The ammonia oxidizing bacteria nutrient solution of CFU/mL is added in mixed liquor R,
Stirred 3 minutes under the conditions of 1000r/min, obtain mixed liquor S；
(27) substance C is added in mixed liquor S, when shake 24 is small in the shaking table that temperature is 35 DEG C, is filtered to remove liquid
Body obtains material D；
(28) when by material D, placement 8 is small in the incubator that temperature is 25 DEG C, obtained material is that fixed ammoxidation is thin
The acrylic resin composite plate of bacterium.
The invention has the advantages that the acrylic resin composite plate of obtained fixed ammonia oxidizing bacteria has strain activity
The function of ammonia nitrogen in high, energy efficient degradation waste water.
Embodiment
The present invention provides a kind of acrylic resin composite plate of fixed ammonia oxidizing bacteria and preparation method thereof, below by
One example illustrates to apply process in fact.
Embodiment 1.
(1) the acrylic resin plate that length, width and thickness are respectively 10cm, 5cm and 0.5cm is added to 250mL matter
Measure in the sulfuric acid solution that percent concentration is 15%, stirred 5 minutes under the conditions of 1000r/min, be filtered to remove liquid, pass through
Placed 120 minutes in the drying box that temperature is 65 DEG C after the washing of 800mL deionized waters, obtain substance A；
(2) 6.3 grams of potassium stannates are added in 750mL deionized waters, stir 3 minutes, shake up under the conditions of 1000r/min
After be divided into 5 parts of equivalent, obtain mixed liquor M1, mixed liquor M2, mixed liquor M3, mixed liquor M4, mixed liquor M5；
(3) 4.4 grams of 2,5- dihydroxy benzenes sulfonic acids potassium and 5.7 grams of trifluoroacetic acid potassium are added in 250mL deionized waters,
Stirred 3 minutes under the conditions of 1000r/min, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor N1, mixed liquor N2, mixed liquor N3, mixed
Close liquid N4, mixed liquor N5；
(4) mixed liquor M1 and 1.9 grams of potassium hydrogen tartrates are added in mixed liquor N1,3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor O1；
(5) substance A is added in mixed liquor O1, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Body obtains substance A 1, and substance A 1 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 2；
(6) mixed liquor M2 and 1.7 grams of potassium hydrogen tartrates are added in mixed liquor N2,3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor O2；
(7) substance A 2 is added in mixed liquor O2, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Body obtains substance A 3, and substance A 3 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 4；
(8) mixed liquor M3 and 1.5 grams of potassium hydrogen tartrates are added in mixed liquor N3,3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor O3；
(9) substance A 4 is added in mixed liquor O3, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Body obtains substance A 5, and substance A 5 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 6；
(10) mixed liquor M4 and 1.3 grams of potassium hydrogen tartrates are added in mixed liquor N4, are stirred under the conditions of 1000r/min
3 minutes, obtain mixed liquor O4；
(11) substance A 6 is added in mixed liquor O4, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance A 7, and substance A 7 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 8；
(12) mixed liquor M5 and 1.1 grams of potassium hydrogen tartrates are added in mixed liquor N5, are stirred under the conditions of 1000r/min
3 minutes, obtain mixed liquor O5；
(13) substance A 8 is added in mixed liquor O5, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance A 9, and substance A 9 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B；
(14) 8.19 grams of potassium tetroxalate dihydrates are added in 1500mL deionized waters under 1000r/min stirring conditions,
Stir 3 minutes under the conditions of 1000r/min, then add 3.3 grams of potassium methoxides, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor P1,
Mixed liquor P2, mixed liquor P3, mixed liquor P4, mixed liquor P5；
(15) 8.8 grams of agaroses and 6.4 Keshan potassium sorbates are added in mixed liquor P1, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q1；
(16) substance B is added in mixed liquor Q1, is shaken 25 minutes in the shaking table that temperature is 38 DEG C, be filtered to remove liquid
Body obtains substance B 1, and substance B 1 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 2；
(17) 8.2 grams of agaroses and 6.1 Keshan potassium sorbates are added in mixed liquor P2, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q2；
(18) substance B 2 is added in mixed liquor Q2, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 3, and substance B 3 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 4；
(19) 7.6 grams of agaroses and 5.8 Keshan potassium sorbates are added in mixed liquor P3, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q3；
(20) substance B 4 is added in mixed liquor Q3, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 5, and substance B 5 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 6；
(21) 7.0 grams of agaroses and 5.5 Keshan potassium sorbates are added in mixed liquor P4, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q4；
(22) substance B 6 is added in mixed liquor Q4, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 7, and substance B 7 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 8；
(23) 6.4 grams of agaroses and 5.2 Keshan potassium sorbates are added in mixed liquor P5, are stirred under the conditions of 1000r/min
Mix 3 minutes, obtain mixed liquor Q5；
(24) substance B 8 is added in mixed liquor Q5, shakes 20 minutes, be filtered to remove in the shaking table that temperature is 45 DEG C
Liquid obtains substance B 9, and substance B 9 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance C；
(25) by 1.8 grams of NH4NO3, 10.2 grams of NaCl, 2.3 grams of KH2PO4, 0.5 gram of FeCl3, 0.3 gram of CoCl2, 0.2 gram
ZnCl2, 3.3 grams of MgSO4, 1.6 grams of CaCl2With 0.1 gram of NiCl2It is added in 1000mL deionized waters, in 1000r/min conditions
Lower stirring 3 minutes, obtains mixed liquor R；
(26) it is 5 × 10 by 10mL bacteria containing amounts12The ammonia oxidizing bacteria nutrient solution of CFU/mL is added in mixed liquor R,
Stirred 3 minutes under the conditions of 1000r/min, obtain mixed liquor S；
(27) substance C is added in mixed liquor S, when shake 24 is small in the shaking table that temperature is 35 DEG C, is filtered to remove liquid
Body obtains material D；
(28) when by material D, placement 8 is small in the incubator that temperature is 25 DEG C, obtained material is that fixed ammoxidation is thin
The acrylic resin composite plate of bacterium.
With fixing the acrylic resin composite plate of ammonia oxidizing bacteria made from the method for the present invention to the waste water containing ammonia nitrogen
Carry out degradation experiment, the results showed that：When the initial concentration of ammonia nitrogen is 99.6mg/L, poly- the third of fixed ammonia oxidizing bacteria is added
The concentration of ammonia nitrogen is reduced to 2.6mg/L after when olefine resin composite plate 4 is small.
Claims (1)
1. a kind of acrylic resin composite plate of fixed ammonia oxidizing bacteria, it is characterised in that prepare the fixation ammonia oxidizing bacteria
Acrylic resin composite plate method comprises the following steps that：
(1) the acrylic resin plate that length, width and thickness are respectively 10cm, 5cm and 0.5cm is added to 250mL mass hundred
Divide in the sulfuric acid solution that specific concentration is 15%, stirred 5 minutes under the conditions of 1000r/min, be filtered to remove liquid, gone through 800mL
Placed 120 minutes in the drying box that temperature is 65 DEG C after ion water washing, obtain substance A；
(2) 6.3 grams of potassium stannates are added in 750mL deionized waters, are stirred 3 minutes under the conditions of 1000r/min, divided after shaking up
Into 5 parts of equivalent, mixed liquor M1, mixed liquor M2, mixed liquor M3, mixed liquor M4, mixed liquor M5 are obtained；
(3) 4.4 grams of 2,5- dihydroxy benzenes sulfonic acids potassium and 5.7 grams of trifluoroacetic acid potassium are added in 250mL deionized waters,
Stirred 3 minutes under the conditions of 1000r/min, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor N1, mixed liquor N2, mixed liquor N3, mixed
Close liquid N4, mixed liquor N5；
(4) mixed liquor M1 and 1.9 grams of potassium hydrogen tartrates are added in mixed liquor N1,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor O1；
(5) substance A is added in mixed liquor O1, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid and obtain
To substance A 1, substance A 1 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 2；
(6) mixed liquor M2 and 1.7 grams of potassium hydrogen tartrates are added in mixed liquor N2,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor O2；
(7) substance A 2 is added in mixed liquor O2, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid and obtain
To substance A 3, substance A 3 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 4；
(8) mixed liquor M3 and 1.5 grams of potassium hydrogen tartrates are added in mixed liquor N3,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor O3；
(9) substance A 4 is added in mixed liquor O3, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid and obtain
To substance A 5, substance A 5 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 6；
(10) mixed liquor M4 and 1.3 grams of potassium hydrogen tartrates are added in mixed liquor N4,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor O4；
(11) substance A 6 is added in mixed liquor O4, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Substance A 7 is obtained, substance A 7 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance A 8；
(12) mixed liquor M5 and 1.1 grams of potassium hydrogen tartrates are added in mixed liquor N5,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor O5；
(13) substance A 8 is added in mixed liquor O5, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Substance A 9 is obtained, substance A 9 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B；
(14) 8.19 grams of potassium tetroxalate dihydrates are added in 1500mL deionized waters under 1000r/min stirring conditions,
Stir 3 minutes under the conditions of 1000r/min, then add 3.3 grams of potassium methoxides, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor P1,
Mixed liquor P2, mixed liquor P3, mixed liquor P4, mixed liquor P5；
(15) 8.8 grams of agaroses and 6.4 Keshan potassium sorbates are added in mixed liquor P1,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q1；
(16) substance B is added in mixed liquor Q1, is shaken 25 minutes in the shaking table that temperature is 38 DEG C, be filtered to remove liquid and obtain
To substance B 1, substance B 1 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 2；
(17) 8.2 grams of agaroses and 6.1 Keshan potassium sorbates are added in mixed liquor P2,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q2；
(18) substance B 2 is added in mixed liquor Q2, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Substance B 3 is obtained, substance B 3 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 4；
(19) 7.6 grams of agaroses and 5.8 Keshan potassium sorbates are added in mixed liquor P3,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q3；
(20) substance B 4 is added in mixed liquor Q3, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Substance B 5 is obtained, substance B 5 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 6；
(21) 7.0 grams of agaroses and 5.5 Keshan potassium sorbates are added in mixed liquor P4,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q4；
(22) substance B 6 is added in mixed liquor Q4, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Substance B 7 is obtained, substance B 7 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance B 8；
(23) 6.4 grams of agaroses and 5.2 Keshan potassium sorbates are added in mixed liquor P5,3 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q5；
(24) substance B 8 is added in mixed liquor Q5, is shaken 20 minutes in the shaking table that temperature is 45 DEG C, be filtered to remove liquid
Substance B 9 is obtained, substance B 9 is placed 120 minutes in the drying box that temperature is 65 DEG C, obtains substance C；
(25) by 1.8 grams of NH4NO3, 10.2 grams of NaCl, 2.3 grams of KH2PO4, 0.5 gram of FeCl3, 0.3 gram of CoCl2, 0.2 gram of ZnCl2、
3.3 grams of MgSO4, 1.6 grams of CaCl2With 0.1 gram of NiCl2It is added in 1000mL deionized waters, 3 is stirred under the conditions of 1000r/min
Minute, obtain mixed liquor R；
(26) it is 5 × 10 by 10mL bacteria containing amounts12The ammonia oxidizing bacteria nutrient solution of CFU/mL is added in mixed liquor R, in 1000r/
Stirred 3 minutes under the conditions of min, obtain mixed liquor S；
(27) substance C is added in mixed liquor S, when shake 24 is small in the shaking table that temperature is 35 DEG C, is filtered to remove liquid and obtains
To material D；
(28) when by material D, placement 8 is small in the incubator that temperature is 25 DEG C, obtained material is fixed ammonia oxidizing bacteria
Acrylic resin composite plate.
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| EP1780271A1 (en) * | 2005-11-01 | 2007-05-02 | Hitachi Plant Technologies, Ltd. | Entrapping immobilization pellets of anaerobic ammonium oxidizing bacteria and process for producing the same |
| CN102398992A (en) * | 2010-09-08 | 2012-04-04 | 吉林省安能科技产业有限公司 | Ammonia-oxidizing bacteria immobilization method employing composite carrier |
| CN104651342A (en) * | 2015-02-17 | 2015-05-27 | 中国海洋大学 | Salt-tolerant cold-resistant ammonia oxidizing bacterium immobilizing method and application thereof |
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| CN107338236A (en) * | 2017-07-30 | 2017-11-10 | 光合强化（北京）生物科技有限公司 | The functional material for being used to fix Azodrin degradation bacteria being modified based on acrylic resin |
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| CN104651342A (en) * | 2015-02-17 | 2015-05-27 | 中国海洋大学 | Salt-tolerant cold-resistant ammonia oxidizing bacterium immobilizing method and application thereof |
| CN105861479A (en) * | 2016-04-25 | 2016-08-17 | 北京工业大学 | Method for co-immobilizing anaerobic ammoxidation bacteria-short-cut nitrobacteria and application thereof |
| CN107338236A (en) * | 2017-07-30 | 2017-11-10 | 光合强化（北京）生物科技有限公司 | The functional material for being used to fix Azodrin degradation bacteria being modified based on acrylic resin |
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