CN1023679C - Production method for Qingzhou Jew's ear - Google Patents

Production method for Qingzhou Jew's ear Download PDF

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Publication number
CN1023679C
CN1023679C CN 90105272 CN90105272A CN1023679C CN 1023679 C CN1023679 C CN 1023679C CN 90105272 CN90105272 CN 90105272 CN 90105272 A CN90105272 A CN 90105272A CN 1023679 C CN1023679 C CN 1023679C
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qingzhou
ear
jew
medium
add
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CN 90105272
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CN1047007A (en
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张文阁
张冰华
张钧华
张颂华
徐洪昌
李国华
张冀华
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QINGZHOU CITY EDIBLE FUNGI INST SHANDONG PROV
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QINGZHOU CITY EDIBLE FUNGI INST SHANDONG PROV
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Abstract

The present invention relates to a production method of Qingzhou jelly fungi. The strain of the Qingzhou jelly fungi is introduced into the cultivation media with strong alkalinity for cultivation; in order to strengthen the medical care function of the jelly fungi, in the cultivation process, the Qingzhou jelly fungi are processed once more by the measures such as traditional Chinese medicine liquid insufflation, etc.; thus, the Qingzhou jelly fungi have the advantages of strong stress resistance and difficult pollution in production processes and have the characteristics of high protein content (more than 13%), exquisite quality, a large amount of contained gelatine and obvious therapeutic effect on hyperlipidemia per se.

Description

Production method for Qingzhou Jew's ear
The present invention is the production method of a kind of edible mushroom, the production method of the more definite Qingzhou Jew's ear of saying so.
Auricularia auriculajudae is a kind of of edible mushroom, and it is a traditional food, the use of can being used as medicine again.The tradition of auricularia auriculajudae is introduced edible fungus in slant acidity or the neutral medium for the material production method and is cultivated, and the shortcoming that said method exists is can not grow in alkaline medium procreation, the auricularia auriculajudae resistance difference of production and easy pollution of edible fungus.
Task of the present invention be to study successfully a kind of utilize the Qingzhou Jew's ear bacterial classification in than the strong basicity medium, cultivate and in cultivating process again by the method for spraying herb liquid produce rich in proteins (more than 13%), quality exquisiteness, strong stress resistance, to be difficult for polluting, contain colloid many and hyperlipidemia is had a Qingzhou Jew's ear of significant curative effect.
Particular content of the present invention:
One. the bacterial classification that the present invention uses: the Qingzhou Jew's ear bacterial strain of this institute acclimatization culture belongs to the Uricularia polytricha subspecies.
Two. the medium that the present invention uses:
1. medium is with material name and quality requirement thereof:
(1) wood chip: generally adopting hardwood sawdust, is optimum with the wood chip with poplar, birch, mulberry tree wherein: the wood chip quality requirement: do not have and go mouldy free from admixture.
(2) cotton seed hull: the requirement nothing is gone mouldy, free from admixture.
(3) maize cob meal, the requirement nothing is gone mouldy, free from admixture.
(4) wheat bran: the requirement nothing is gone mouldy, free from admixture.
(5) composite fertilizer: the composite fertilizer that requires nitrogenous, phosphorus, potassium.
(6) white lime: require free from admixture, water content is below 40%.
(7) corn flour: require free from admixture.
2. medium is with the formula rate of raw material:
(1) general formula rate:
Wood chip: 30-40%: cotton seed hull: 51-33%; Maize cob meal: 8-10%; Corn flour: 5-8%; Wheat bran: 5-8%; Composite fertilizer: 1%; White lime: an amount of.
(2) optimum formula ratio:
Wood chip: 35%, cotton seed hull: 39%, maize cob meal: 10%; Corn flour: 8%, wheat bran: 7%, composite fertilizer 1%, white lime: an amount of.
Three. concrete production process of the present invention:
After bacterial classification and raw material are got all the ready by above-mentioned requirements, produce by following step again:
1. allotment medium:
Press earlier the culture medium prescription proportion requirement, weigh up medium is raw materials used, and then in material: the ratio of water=1: 1.3 adds entry (running water) and stirs, when stirring, add an amount of water content at the white lime below 40%, stir and add in the process of white lime and require the material temperature preferably to be controlled at 15-30 ℃ of scope adding water, the white lime addition will just be decided on its material temperature: 10 ℃ of following additions are 10%; Addition is 12% in the time of 11-15 ℃, and addition is 15% in the time of 16-25 ℃, and addition is 18% in the time of 25-30 ℃, and the material that adds white lime must stir evenly and change down the step again over to.
2. pack, sterilization: medium is after adding white lime and stirring evenly, earlier (for example: polyethylene) or in the wide-mouth bottle with the plastic sack of its 14X33 centimetre of both ends open of packing into, the loading amount of each plastic sack is the 400-500 gram, then with plastic sack openings at two ends good seal, carry out normal pressure (100 ℃, 10-12 hour) or high pressure (vapour pressure is 1.5 gauge pressures, 3-3.5 hour) sterilization, material after the sterilization will drop to below 30 ℃, and storage is good standby.
3. inoculation, cultivation: also reducing to the two ends that room temperature (below 30 ℃) is equipped with the plastic sack of medium after the sterilization, the mouth of good seal is opened access Qingzhou Jew's ear bacterial classification, bacterial classification access amount: it is 0.5-1.5 centimetre bacterium piece 2-3 that every end of plastic sack will be put into diameter, connect and planted again with the sack good seal, then plastic sack is crouched and put, and single one by one yard, carry out the discharging of wall formula, under 15-30 ℃, carry out lucifuge and cultivate.
4. carry out the equation of light, temperature difference management: when mycelia is covered with.When ripe, on the bacterium wall that the plastic sack sign indicating number rises, cover one deck shade, only allow the plastic sack two ends be subjected to light, budding from the plastic sack two ends in order to it like this.When original hase appears in the sack two ends, open the two ends sack, and add intense light irradiation (illuminance is the 300-2000 lux) and ventilate (will open the window every day and ventilate more than 3 times), ventilation also increases with the increase of auricle.(relative moisture is: 90-95%) also will to increase air humidity when adding intense light irradiation and ventilation.
5. spray liquid: " carrying out the equation of light, temperature difference management " in the stage, when auricle is grown up when diameter reaches 5 centimetres, along with the increase of ventilation also will be carried out spray concentration respectively and is equal to or less than the gibberellin aqueous solution that 0.5% the Ligusticum wallichii Chinese medicine aqueous solution and concentration are 10-20ppm.The concrete grammar of spray liquid is: spray above-mentioned soup with sprayer on auricle, every day is more than 3 times.Will note during spray liquid: what young ear spilt will lack, and what become that ear spills wants many, when temperature is lower than 15 ℃ or air humidity more than 95%, or does not want spray liquid the ventilation condition difference time.
6. the harvesting of auricularia auriculajudae: grow up when the edge undulation being arranged and have spore to launch when the auricle of auricularia auriculajudae, can pluck auricularia auriculajudae.After drying, auricularia auriculajudae under plucking is the finished product that the present invention produces.
7. recuperation of mycelia and recovery:
After auricularia auriculajudae is gathered, cut off the water 5-8 days, during cutting off the water, strengthen ventilation and illumination to the ear canopy, and in canopy, spray one time with 5% limewash, to prevent insect pest and to dye, bacterium grows and spreads, pass through 5-8 days when the mycelia recuperation recovers, spray concentration is 0.5% nitrogenous on the sack of plastic sack two ends, phosphorus, twice of the composite fertilizer of potassium, the pH value that be to keep medium after the water on the sack is done, is sprayed 5% limewash supernatant on the sack of two ends twice again, then with the sack good seal, carry out the mycelia lucifuge and cultivate, condition of culture is the same, after buddingging, as previously mentioned, carry out the equation of light, temperature difference management, spray liquid, harvesting of auricularia auriculajudae and the recuperation of mycelia and recovery several steps are produced Qingzhou Jew's ear.Medium in the plastic sack can only use 3-4 time when the duplication of production auricularia auriculajudae.
Advantage of the present invention and characteristics:
One. medium of the present invention is more alkaline medium.
Traditional auricularia auriculajudae cultural method is to use slant acidity or neutral medium, and the medium that the present invention uses is owing to added the white lime 5-18% that contains 40% following moisture, so medium presents than strong basicity, the Qingzhou Jew's ear bacterial classification is introduced in this medium, made it grow the measured auricularia auriculajudae of matter by a series of control measures.
Two. the present invention sprays herb liquid in producing the Qingzhou Jew's ear process, strengthened the medical functions of Qingzhou Jew's ear:
According to the elaboration of " each wood of auricularia auriculajudae is all living, and its good poison also must be with wood ", the present invention except that in medium with the poplar bits.Also in process of production, when the auricle diameter grows to 5 centimetres, spray the herb liquid number time that contains the Ligusticum wallichii composition every day, so just strengthened the function of the auricularia auriculajudae treatment hyperlipidemia that the present invention produces.
Three. Qingzhou Jew's ear strong stress resistance that the present invention produces and difficult the pollution:
Even stream ear phenomenon also often appears in common woodear in the good fluffy cellar for storing things of condition, go through evil sometimes even very, and just easily go mouldy when temperature rises to 28 ℃; But the Qingzhou Jew's ear that the present invention produces is all rare stream ear phenomenon in fluffy kiln or in the woods no matter, again because of Qingzhou Jew's ear is difficult for pollution, so also do not go mouldy even temperature rises to 36-37 ℃.
Four. Qingzhou Jew's ear protein content height and the more common auricularia auriculajudae exquisiteness of auricularia auriculajudae quality that the present invention produces, colloid is many especially:
National Specification auricularia auriculajudae protein content is more than 7.7%.Common auricularia auriculajudae protein content is after testing all in the 7-9% scope, and the Qingzhou Jew's ear protein content that the present invention produces is through Shandong University, its protein content detects all at (during auricularia auriculajudae water content 12%) more than 13% in the many families in Qingzhou City food quality inspection station and food quality inspection station, Shandong Province, surpasses more than 68% of national regulation minimum standard; Exceed 50% than other auricularia auriculajudae protein contents, the Qingzhou Jew's ear that the present invention produces is also fine and smooth and to contain colloid many because of the general auricularia auriculajudae of its quality, so stick-slip is good to eat in cooking.
Five. the Qingzhou Jew's ear that the present invention produces has significant curative effect to hyperlipidemia:
The high-grade nutrient food of still not a kind of rich in proteins of Qingzhou Jew's ear (more than 13%) that the present invention produces, and be a kind of herbal cuisine that hyperlipidemia is had significant curative effect.Qingzhou Jew's ear suffers from various degree by 140 that the hyperlipidemia patient takes, and the effect of degree varies is all arranged, and only exemplifies the situation that seven patients take Qingzhou Jew's ear below its curative effect is described: (one). test site: 402 hospitals of the Chinese People's Liberation Army
(2). test method:
1. Qingzhou Jew's ear consumption: every day 3 times, cooking is eaten, consumption per day 45-50 gram.
2.40 it is one-period, has a blood test the contrast (respectively having a blood test once eventually in 20 days and 40 days) of having a blood test again that is in 40 days before the test.
3. take during the Qingzhou Jew's ear all blood lipid-lowering medicines of stopping using.
(3). result of the test
(table is seen the literary composition back)
Shown by the tabular result of the test: the effect for reducing blood fat of Qingzhou Jew's ear is apparent in view, and is especially more remarkable to high cholesterol patient effect.
Embodiment:
One. bacterial classification: Qingzhou Jew's ear bacterial strain
Two. medium: allocate medium: willow wood chip 35% by following formula rate, maize cob meal 10%, corn flour 5%, wheat bran 8%, composite fertilizer's (containing N.P.K) 1%, cotton seed hull 41%, totally 1200 kilograms of inventorys, in material: the ratio of water=1: 1.3 adds water and is mixed, and then to add water content in per hundred kilograms of siccatives be that the ratio of 15 kilograms of 35% white limes adds white lime and stirs evenly, behind the mixing material packed in the high pressure polyethylene plastic bag of 14 * 33 centimetres of both ends opens, under 100 ± 1 ℃, the sterilization through 10 hours, after the sterilization when temperature is reduced to 30 ℃, the plastic sack two-port is opened, the Qingzhou Jew's ear bacterial classification is inserted in the medium.The inoculation method of bacterial classification and access amount: insert bacterial classification at the two ends of plastic sack, it is 0.5-1.5 centimetre bacterium piece 2-3 piece that each termination of bag is gone into diameter.After bacterial classification connects, again the two ends sack is sealed, under the lucifuge condition, cultivate, increase foster temperature and will be controlled at 15 ° of-30 ℃ of scopes, after auricle stretches air humidity is transferred to 90-95%, illumination is controlled at the 300-2000 lux and windows every day ventilates more than 3 times: when the auricle diameter extended to 5 centimetres, temperature was more than 20 ± 1 ℃ the time, spray 2-4 time respectively with the Ligusticum wallichii herb liquid of 0.5% concentration and the gibberellin soup of 20ppm every day, plucks to auricularia auriculajudae and adopt the eve and stop to spray liquid medicine again; Can pluck when having spore to distribute, the auricularia auriculajudae under plucking is through drying the finished product that is present embodiment.
The auricularia auriculajudae of present embodiment production detects through relevant department, and its protein content is 13.3%, and prove by 7 routine hyperlipidemia patients' the clinical result of taking through 402 hospitals of the Chinese People's Liberation Army: Qingzhou Jew's ear has obvious curative effects to hyperlipidemia; Especially the high-cholesterol disease patient more there is significant curative effect (test data is seen before, and face is routine to be shown).
(3). result of the test:
Cholesterol (mg%) triglyceride (mg%) high-density lipoprotein (HDL)
The name age-sex
After preceding treatment is treated in the treatment back before treating after the treatment before the treatment
(1)155 (1)197 (1)32
High day good fortune 55 men 161 (2) 158 400 (2) 107 32 (2) 32
Wang Chenglin 57 men 226 192 240 117 46 42
Zhao ancestor literary composition 59 men 176 171 142 89 45 54
Annotate emerging 55 man 154 154 336 217 32 35
History chief space 55 men 247 196 89 109 55 42
Go through and build cloud 50 woman 267 196 127 147 80 54
Yan Min 54 woman 241 216 204 326 53 45

Claims (3)

1, the production method of Qingzhou Jew's ear comprises: (1) allotment medium, (2) pack, sterilization, (3) inoculation, cultivate, (4) carry out the equation of light, temperature difference management, (5) plucking of auricularia auriculajudae adopted, and recuperation of (6) mycelia and recovery several steps is characterized in that the used bacterial classification of this method is the Qingzhou Jew's ear bacterial classification, with culture medium prescription be: wood chip 30~40%, cotton seed hull 33~51%, maize cob meal 8~10%, corn flour 5~8%, wheat bran 5~8%, composite fertilizer 1%, and in this medium, will add water content and be not more than 40% white lime, addition is respectively: add 10% of quite above-mentioned formula ratio below 10 ℃, 11~15 ℃ add 12%, 16~25 ℃ and add 15%; 25~30 ℃ add 18%; There is step (4) to carry out also wanting spray liquid in the equation of light, the temperature difference management process again in this method.
2, according to the production method of the described Qingzhou Jew's ear of claim 1, the concocting method that it is characterized in that the described medium of this method is earlier in the culture medium prescription ratio, the raw material that medium is used weighs up, and then in material: the ratio of water=1: 1.3 adds entry and stirs, when stirring according under different temperatures, adding the water content of different amounts at the white lime below 40%.At Jia Shui, stir and add in the process of white lime, require the material temperature control in 15~30 ℃ of scopes.
3, according to the production method of the described Qingzhou Jew's ear of claim 1, it is characterized in that the step (4) in this method carries out having carried out again in the equation of light, the temperature difference management process spray liquid, be meant when auricle is grown up to 5 centimetres, along with the increase of ventilation, also to carry out spray concentration and be not more than the gibberellin aqueous solution that 0.5% the Ligusticum wallichii Chinese medicine aqueous solution and concentration are 10-20PPM.The concrete grammar of spray liquid is: with sprayer to spray liquid every day on the auricle more than 3 times.That will notice during sprinkling that young ear spills will lack, and what become that ear spills wants many; And have only the temperature degree in 15 ℃, or the air relative temperature is lower than at 95% o'clock and ventilation condition when better, just can carry out spray liquid.
CN 90105272 1990-04-23 1990-04-23 Production method for Qingzhou Jew's ear Expired - Fee Related CN1023679C (en)

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* Cited by examiner, † Cited by third party
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CN1047200C (en) * 1994-03-09 1999-12-08 刘永昶 Auricularia auricula-judae cultivated in plastic bag substitute material field and cultivation method
CN103214279A (en) * 2013-04-22 2013-07-24 邬金飞 Compatibility of black fungus cultivation material and manufacturing method of cultivation material
CN103460992B (en) * 2013-08-23 2015-07-22 福建省农业科学院农业工程技术研究所 Method for cultivating agaric by using pig feces bag
CN104311284A (en) * 2014-09-26 2015-01-28 侯中仁 Preparation method of black fungus culture medium
CN104909894A (en) * 2015-06-03 2015-09-16 广西大学 Black fungus culture medium and preparation method thereof
CN105165402A (en) * 2015-09-24 2015-12-23 龙里卓驰农业科技有限公司 Method for cultivating edible fungus in kitchen
CN105638239A (en) * 2015-12-31 2016-06-08 钦州市钦北区生产力促进中心 Efficient black fungus cultivation method
CN106083230A (en) * 2016-06-22 2016-11-09 潘雪玉 Reduce the ecological cultivation method of content of beary metal in Auricularia
CN106069191A (en) * 2016-06-22 2016-11-09 潘雪玉 The bacterium bag cultivating method of Auricularia
CN105961030A (en) * 2016-06-22 2016-09-28 潘雪玉 Black fungus cultivation method
CN106069190A (en) * 2016-06-22 2016-11-09 潘雪玉 The steam bacterium bag cultivation method of Auricularia
CN105961031A (en) * 2016-06-22 2016-09-28 潘雪玉 High-yield black fungus cultivation method
CN106069194B (en) * 2016-06-29 2020-05-19 金皓 Method for cultivating lucid ganoderma and collecting lucid ganoderma spore powder
CN105936598A (en) * 2016-07-04 2016-09-14 贵州根生科技农业有限公司 Culture material for edible fungus and preparation method thereof
CN110915548A (en) * 2019-12-06 2020-03-27 安徽农业大学 Auricularia auricula bagged culture material additive and using method thereof

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