CN105638239A - Efficient black fungus cultivation method - Google Patents

Efficient black fungus cultivation method Download PDF

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Publication number
CN105638239A
CN105638239A CN201511021879.9A CN201511021879A CN105638239A CN 105638239 A CN105638239 A CN 105638239A CN 201511021879 A CN201511021879 A CN 201511021879A CN 105638239 A CN105638239 A CN 105638239A
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black fungus
inoculation
temperature
parts
auricle
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CN201511021879.9A
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陈锋
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Qinzhou Qinbei Productivity Promotion Center
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Qinzhou Qinbei Productivity Promotion Center
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Priority to CN201511021879.9A priority Critical patent/CN105638239A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides an efficient black fungus cultivation method. The method comprises the following steps of 1, compost preparation; 2, bagging sterilization and inoculation; 3, thermal insulation and spawn running; 4, fungus emergence management; 5, harvesting. The scientific and reasonable cultivation method is adopted, and the method is simple and easy to implement; according to the black fungus cultivated through the cultivation method, the growth period can be shortened by 10-15 d, economic benefits are improved remarkably, the yield and quality of the black fungus are improved at the same time, the yield of black fungus can be improved by 40% or above compared with existing black fungus cultivated through a culture medium, the purpose of increasing both production and income is achieved, and the method is worthy of wide popularization.

Description

The efficient cultivation method of a kind of black fungus
Technical field
The present invention relates to Chinese herbal medicine cultivation technology field, it is specifically related to the efficient cultivation method of a kind of black fungus.
Background technology
Black fungus, also known as auricularia auriculajudae, brightness program, cloud ear, black dish. On taxonomy, black fungus belongs to Mycophyta, Basidiomycotina, Hymenomycetes, auricularia auriculajudae order, auricularia auriculajudae section, Auricularia, is the traditional large edible mushrooms kind of China. Extensive in China and even distribution on global, particularly in remote mountains area, Lushan County Henan Province, there is a large amount of wild populations, germ plasm resource is enriched. Black fungus has brain healthy, it is strong to nourish, enrich blood and control blood, nourshing Yin and drynsessmoistening prescription, nourishing the stomach defaecation, clearing lung-heat benefit effect such as gas, sedation-analgesia. Containing abundant Mierocrystalline cellulose and a kind of special plant colloid in black fungus, gastrointestinal peristalsis can be promoted; Impel the excretion of enteron aisle fat food, reduce the absorption of food fat, thus play antiobesity action. Colloid in black fungus, have moistening lung and wash clearly stomach and intestine effect can by the impurity remaining in digestive tube, refuse absorption excrete, therefore it is also one of important protective foods of textile worker and mine worker. Polysaccharide in black fungus has antitumous effect, it is possible to as the dietotherapy of tumour patient.
Black fungus major part in existing market adopts artificial cultivation technique, owing to black fungus is to temperature, the ambient conditionss such as humidity require strict, cause auricularia auriculajudae output low, in addition, it is roughly the same that method cultivated by current black fungus, by wood chip, cotton sub-skin, crop stalk crushed material, wheat bran, mineral substance etc. load in special high temperature resistant plastics bag, through High Temperature Sterilization, inoculation, cultivate, scarfing goes out ear, when going out ear, some handles go out ear bag and hang in canopy room, some handles go out the setting of ear bag and are placed on the furrow bed of ground, but it is low generally to there is output in extensive cultivation, poor quality, pollution rate height, the problems such as output is unstable, become the bottleneck of cultivating Auricularia auricula-judae in bags development, it is badly in need of being resolved and improving. in addition, at present more than the batching of the culture material of major part black fungus, science, trophic structure are not unreasonable, more do not possess antibacterial, antibacterial, cultivate the auricularia auriculajudae ratio of first-grade products out low.
Summary of the invention
For the deficiencies in the prior art, it is an object of the invention to provide a kind of grow, the efficient cultivating method of black fungus that output height, product are of fine quality.
In order to achieve the above object, the technical scheme that the present invention takes is:
A kind of efficient cultivating method of black fungus, comprises the following steps:
(1) preparation of culture material: take cotton seed hull 65��75 parts, 15��25 parts, rice bran, terra alba 1��5 part, calcium superphosphate 1��5 part, 2��6 parts, lime fully stir, sealing and fermenting 15��20 hours at temperature is 30��40 DEG C;
(2) sterilizing and inoculation is packed: being loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 150��180 DEG C of constant temperature sterilizing 12��15h, is cooled to less than 30 DEG C, accesses bacterial classification under aseptic technique;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, in after inoculation 10 days, temperature control 21��23 DEG C, humidity remain on 60%��70%, after inoculation 10, temperature control 26��29 DEG C, humidity remain on 50%��60%, ventilate every day 25��30min;
(4) ear management is gone out: cover with until mycelia and move into cultivation canopy after bacterium bag, control temperature 15��20 DEG C, atmospheric moisture is 75%��85%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, sprays a small amount of water toward bacterium bag simultaneously, keeps atmospheric moisture 86��95%, when auricle launches, atmospheric moisture control 60%��70%;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
As optimal technical scheme, suitable in order to ensure to connect bacterium amount, be conducive to mycelial growth, avoid the waste of bacterial classification simultaneously, in described step (2), the inoculum size of every bag is 5��10g.
As optimal technical scheme, in order to ensure sterilizing bag arrangement rationally, to be conducive to improving a bacterium rate of seed, thus improving the Quality and yield of product, the sack after the middle inoculation of described step (3) piles " well " font or trilateral is placed.
As optimal technical scheme, in order to ensure to provide the nutrient of abundance, be conducive to improving the seed output and quality of product, when in described step (4), auricle launches, potassium primary phosphate, the urea soln of 5% with 1��3% spray auricle, spray 1 time every other day, continuous 3��4 times.
The useful effect that the present invention has: the present invention adopts the preparation of culture material, pack sterilizing and inoculation, insulation to send out a bacterium, go out ear manages, this set of scientific and reasonable cultivating method of gathering, method is simple, be easy to operation, with the cultivation method black fungus of the present invention, growth cycle can be shortened 10��15 days, significantly improve economic benefit, improve the seed output and quality of black fungus simultaneously, the output increased more than 40% that the culture medium culturing comparing existing black fungus obtains, realize the object of increasing both production and income, should be widely promoted.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
A kind of efficient cultivating method of black fungus, comprises the following steps:
(1) preparation of culture material: take cotton seed hull 65 parts, 25 parts, rice bran, terra alba 2 parts, calcium superphosphate 2 parts, 6 parts, lime fully stir, sealing and fermenting 15 hours at temperature is 30 DEG C;
(2) sterilizing and inoculation is packed: being loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 150 DEG C of constant temperature sterilizing 12h, is cooled to less than 30 DEG C, accesses bacterial classification under aseptic technique;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, in after inoculation 10 days, temperature control 21 DEG C, humidity remain on 60%, inoculation 10 after, temperature control 26 DEG C, humidity remain on 50%, ventilate every day 25min;
(4) ear management is gone out: covering with until mycelia and move into cultivation canopy after bacterium bag, control temperature 15 DEG C, atmospheric moisture is 75%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, sprays a small amount of water toward bacterium bag simultaneously, keep atmospheric moisture 86%, when auricle launches, atmospheric moisture control 60%%;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
Embodiment 2
A kind of efficient cultivating method of black fungus, comprises the following steps:
(1) preparation of culture material: take cotton seed hull 68 parts, 23 parts, rice bran, terra alba 2 parts, calcium superphosphate 2 parts, 5 parts, lime fully stir, sealing and fermenting 17 hours at temperature is 32 DEG C;
(2) sterilizing and inoculation is packed: loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 155 DEG C of constant temperature sterilizing 13h, being cooled to less than 30 DEG C, access bacterial classification under aseptic technique, the inoculum size of every bag is 5��10g;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, in after inoculation 10 days, temperature control 22 DEG C, humidity remain on 62%, inoculation 10 after, temperature control 27 DEG C, humidity remain on 52%, ventilate every day 26min;
(4) ear management is gone out: covering with until mycelia and move into cultivation canopy after bacterium bag, control temperature 16 DEG C, atmospheric moisture is 78%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, sprays a small amount of water toward bacterium bag simultaneously, keep atmospheric moisture 89%, when auricle launches, atmospheric moisture control 62%;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
Embodiment 3
A kind of efficient cultivating method of black fungus, comprises the following steps:
(1) preparation of culture material: take cotton seed hull 70 parts, 20 parts, rice bran, terra alba 3 parts, calcium superphosphate 3 parts, 4 parts, lime fully stir, sealing and fermenting 18 hours at temperature is 35 DEG C;
(2) sterilizing and inoculation is packed: being loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 170 DEG C of constant temperature sterilizing 14h, is cooled to less than 30 DEG C, accesses bacterial classification under aseptic technique;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, sack piles " well " font or trilateral is placed, in after inoculation 10 days, temperature control 23 DEG C, humidity remain on 65%, after inoculation 10, temperature control 28 DEG C, humidity remain on 55%, ventilate every day 27min;
(4) ear management is gone out: covering with until mycelia and move into cultivation canopy after bacterium bag, control temperature 17 DEG C, atmospheric moisture is 80%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, sprays a small amount of water toward bacterium bag simultaneously, keep atmospheric moisture 90%, when auricle launches, atmospheric moisture control 65%;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
Embodiment 4
A kind of efficient cultivating method of black fungus, comprises the following steps:
(1) preparation of culture material: take cotton seed hull 72 parts, 17 parts, rice bran, terra alba 4 parts, calcium superphosphate 4 parts, 3 parts, lime fully stir, sealing and fermenting 19 hours at temperature is 37 DEG C;
(2) sterilizing and inoculation is packed: being loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 180 DEG C of constant temperature sterilizing 15h, is cooled to less than 30 DEG C, accesses bacterial classification under aseptic technique;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, in after inoculation 10 days, temperature control 21 DEG C, humidity remain on 68%, inoculation 10 after, temperature control 29 DEG C, humidity remain on 58%, ventilate every day 29min;
(4) ear management is gone out: covering with until mycelia and move into cultivation canopy after bacterium bag, control temperature 19 DEG C, atmospheric moisture is 82%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, sprays a small amount of water toward bacterium bag simultaneously, keep atmospheric moisture 92%, when auricle launches, atmospheric moisture control 68%;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
Embodiment 5
A kind of efficient cultivating method of black fungus, comprises the following steps:
(1) preparation of culture material: take cotton seed hull 75 parts, 15 parts, rice bran, terra alba 4 parts, calcium superphosphate 4 parts, 2 parts, lime fully stir, sealing and fermenting 20 hours at temperature is 40 DEG C;
(2) sterilizing and inoculation is packed: being loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 180 DEG C of constant temperature sterilizing 15h, is cooled to less than 30 DEG C, accesses bacterial classification under aseptic technique;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, in after inoculation 10 days, temperature control 23 DEG C, humidity remain on 70%, inoculation 10 after, temperature control 26 DEG C, humidity remain on 60%, ventilate every day 30min;
(4) ear management is gone out: cover with until mycelia and move into cultivation canopy after bacterium bag, control temperature 20 DEG C, atmospheric moisture 85%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, spray a small amount of water toward bacterium bag simultaneously, keep atmospheric moisture 95%, when auricle launches, atmospheric moisture control 70%, spray auricle with the potassium primary phosphate of 1��3%, the urea soln of 5% simultaneously, spray 1 time every other day, continuous 3��4 times;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
It is noted that above case is only the representational example of the present invention. Obviously, the technical scheme of the present invention is not limited to above-described embodiment, it is also possible to have many sex change.

Claims (4)

1. the efficient cultivating method of black fungus, it is characterised in that, comprise the following steps:
(1) preparation of culture material: take cotton seed hull 65��75 parts, 15��25 parts, rice bran, terra alba 1��5 part, calcium superphosphate 1��5 part, 2��6 parts, lime fully stir, sealing and fermenting 15��20 hours at temperature is 30��40 DEG C;
(2) sterilizing and inoculation is packed: being loaded by the culture material prepared in the polyethylene bag that specification is 17cm �� 33cm �� 45cm, being placed in temperature is after 150��180 DEG C of constant temperature sterilizing 12��15h, is cooled to less than 30 DEG C, accesses bacterial classification under aseptic technique;
(3) bacterium is sent out in insulation: be placed in by the sack after inoculation in insulation, ventilation, dry culturing room, in after inoculation 10 days, temperature control 21��23 DEG C, humidity remain on 60%��70%, after inoculation 10, temperature control 26��29 DEG C, humidity remain on 50%��60%, ventilate every day 25��30min;
(4) ear management is gone out: cover with until mycelia and move into cultivation canopy after bacterium bag, control temperature 15��20 DEG C, atmospheric moisture is 75%��85%, when bacterium bag covers with ear bud, even acanthopore on bacterium bag, sprays a small amount of water toward bacterium bag simultaneously, keeps atmospheric moisture 86��95%, when auricle launches, atmospheric moisture control 60%��70%;
(5) gathering: the auricle of black fungus all launches, edge is volume slightly, and color transfers puce to by purplish red, and white spore occurs, back side yellow, fine hair starts to come off, so that it may gather.
2. a kind of efficient cultivating method of black fungus according to claim 1, it is characterised in that: in described step (2), the inoculum size of every bag is 5��10g.
3. a kind of efficient cultivating method of black fungus according to claim 2, it is characterised in that: in described step (3), sack after inoculation piles " well " font or trilateral is placed.
4. a kind of efficient cultivating method of black fungus according to claims 1 to 3 any one, it is characterized in that: when in described step (4), auricle launches, potassium primary phosphate, the urea soln of 5% with 1��3% spray auricle, spray 1 time every other day, continuous 3��4 times.
CN201511021879.9A 2015-12-31 2015-12-31 Efficient black fungus cultivation method Pending CN105638239A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106165615A (en) * 2016-07-04 2016-11-30 贵州根生科技农业有限公司 A kind of cultural method of Auricularia
CN107371806A (en) * 2017-08-21 2017-11-24 佛山推启农业研究院(普通合伙) A kind of cultivation technique of Silicon-rich black fungus
CN107493964A (en) * 2017-08-28 2017-12-22 广西柳城县绿之缘生态农业科技有限公司 A kind of bacterium germination management method of black fungus
CN107821000A (en) * 2017-10-30 2018-03-23 青川山水涧旅游资源开发有限公司 A kind of segment wood cultivated method of gold agaric
CN108849243A (en) * 2018-07-24 2018-11-23 贵州晏山农业发展有限公司 A kind of cultivation of auricularia auricula method
CN109673378A (en) * 2017-09-20 2019-04-26 遵义晨光云农业产业发展有限公司 A kind of implantation methods for black fungus
CN117617059A (en) * 2024-01-10 2024-03-01 延边朝鲜族自治州农业科学院(延边特产研究所) Cultivation material and cultivation method for improving quality of black fungus

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106165615A (en) * 2016-07-04 2016-11-30 贵州根生科技农业有限公司 A kind of cultural method of Auricularia
CN107371806A (en) * 2017-08-21 2017-11-24 佛山推启农业研究院(普通合伙) A kind of cultivation technique of Silicon-rich black fungus
CN107493964A (en) * 2017-08-28 2017-12-22 广西柳城县绿之缘生态农业科技有限公司 A kind of bacterium germination management method of black fungus
CN109673378A (en) * 2017-09-20 2019-04-26 遵义晨光云农业产业发展有限公司 A kind of implantation methods for black fungus
CN109673378B (en) * 2017-09-20 2020-10-09 陕西秦峰农业股份有限公司 Planting method for black fungus
CN107821000A (en) * 2017-10-30 2018-03-23 青川山水涧旅游资源开发有限公司 A kind of segment wood cultivated method of gold agaric
CN108849243A (en) * 2018-07-24 2018-11-23 贵州晏山农业发展有限公司 A kind of cultivation of auricularia auricula method
CN117617059A (en) * 2024-01-10 2024-03-01 延边朝鲜族自治州农业科学院(延边特产研究所) Cultivation material and cultivation method for improving quality of black fungus

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Application publication date: 20160608