CN1663425A - Method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff - Google Patents
Method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff Download PDFInfo
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- CN1663425A CN1663425A CN2005100386135A CN200510038613A CN1663425A CN 1663425 A CN1663425 A CN 1663425A CN 2005100386135 A CN2005100386135 A CN 2005100386135A CN 200510038613 A CN200510038613 A CN 200510038613A CN 1663425 A CN1663425 A CN 1663425A
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 108090000623 proteins and genes Proteins 0.000 title claims description 19
- 102000004169 proteins and genes Human genes 0.000 title claims description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000001913 cellulose Substances 0.000 claims abstract description 22
- 229920002678 cellulose Polymers 0.000 claims abstract description 22
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000004202 carbamide Substances 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims description 33
- 241000235342 Saccharomycetes Species 0.000 claims description 26
- 239000002609 medium Substances 0.000 claims description 24
- 230000001580 bacterial effect Effects 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 13
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 230000004913 activation Effects 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000012266 salt solution Substances 0.000 claims description 10
- 238000011218 seed culture Methods 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 241000228245 Aspergillus niger Species 0.000 claims description 6
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 6
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 5
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 5
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- 238000009423 ventilation Methods 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 abstract description 5
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- 244000005700 microbiome Species 0.000 abstract description 4
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
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- 239000000047 product Substances 0.000 description 7
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- 241001113556 Elodea Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
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- 239000008103 glucose Substances 0.000 description 3
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- 235000019750 Crude protein Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000013370 mutualism Effects 0.000 description 2
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- 235000019698 starch Nutrition 0.000 description 2
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- 241000609240 Ambelania acida Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
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- 108010064851 Plant Proteins Proteins 0.000 description 1
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- 239000010905 bagasse Substances 0.000 description 1
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- 229940106157 cellulase Drugs 0.000 description 1
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- 239000003205 fragrance Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 108010090785 inulinase Proteins 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
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- 239000002023 wood Substances 0.000 description 1
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Abstract
The invention discloses a method of producing feed albumen by that microorganism state ferment hydrophytes, which is that crushed raw materials of hydrophytes or algae contain right water, encased in reactor with adding proper bran and urea after mixed uniformly; after sterilization adding cellulose decomposition mold with weight share 1%~5% to cultivate 12~48 hours, then inoculating microzyme with weight share1%~5% and cultivating 48~96 hours. For selected hydrophytes or algae as raw materials, this invention not only improves ecology environment but also bring economics benefit, and increases clearly albumen quality in product as mutual action of mold and microzyme during the fermentation process.
Description
One, technical field
The present invention relates to a kind of method of microbial solid fermentation, relate in particular to a kind of method of water plant being produced protein feed by microbial solid fermentation.
Two, background technology
Current, in the restoration of the ecosystem work of lake eutrophication, algae type eutrophic lake is adopted the method for the aquatic higher plant of a large amount of artificial growths, replace algae to transform the ecologic structure in lake with pasture and water; Careless type eutrophic lake is then adopted artificial a large amount of harvesting pasture and water, set up the method for nutriments such as the nitrogen phosphorus handover passage in the lake.The comprehensive utilization of resources of a large amount of water plants also only is confined at present as the elementary green forage of fishery with as aspects such as papermaking, building materials raw materials, thereby and exist the population that causes owing to the water plant selective use and degenerate and the utilization rate defective that causes secondary pollution on the low side, easily cause the deterioration of lake water quality, quicken problems such as lacustrine deposit.
Feed is a big and tool industry with broad prospects for development of comprehensive benefit with the production of microprotein.Traditional solid fermentation is produced the method for fermented feed, is widely used in China rural area.It is raw material that conventional method adopts roughages such as cereal bran, bagasse and stalk, utilizes various beneficial microorganism effects in inulinase and the raw material, roughage is processed into the feed of nutrient height, good palatability, to improve the feeding nutritive value of roughage.Studies show that, it is long-pending that fermentation is not that simple roughage adds water-water reactor, but relate to the effect of microorganism complexity, mainly be by aspergillus non-digestible composition to be changed into to give attitude, materials such as crude fibre, starch, pectin are changed into various carbohydrates, utilize the material of the higher and good palatability of the synthetic nutrition of some composition by yeast and lactic acid bacteria, as protein, amino acid, vitamin, organic acid, alcohol etc., and the measured fermented feed of matter has acid, sweet, fragrance road.China's fodder yeast production at present mostly is solid fermentation method, applied raw material mainly is agricultural byproducts and slag dregs of rice class and the industrial residue etc. that contain starch or sugar, does not cultivate the report that the solid state fermentation water plant produces protein feed but use the combined inoculation of many bacterial classifications at present.
Three, summary of the invention
1, goal of the invention: the purpose of this invention is to provide a kind of is raw material with water plant or algae, protein feed production method easy and simple to handle, that processing cost is low and the product protein content is high.
2, technical scheme: in order to realize the foregoing invention purpose, the method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff of the present invention comprises the following steps:
(1) be raw material with the water plant, utilize disintegrating apparatus to pulverize, after the assurance raw material pulverizing, the quality of moisture content is the 50%-70% of raw material quality; Can realize by following proposal: will pulverize behind a part of water plant raw material drying earlier, another part water plant is pulverized under dampness, two parts raw material is mixed again, and the quality that guarantees moisture content in the mixed raw material is the 50%-70% of raw material quality;
(2) press the quality of above-mentioned raw materials, stir after adding the urea of the wheat bran of 10%-20% and 1%-5%, make fermentation medium;
(3) fermentation medium is packed in the fermentation reactor, the cellulose of the 1%-5% of fermentation medium quality is decomposed mould insert and cultivated 12-48 hour, ventilate in the incubation and stir; Fermentation temperature is 25 ℃-32 ℃;
(4) inoculate the fermentation medium quality 1%-5% saccharomycete and cultivated 48 hours to 96 hours, under ventilation state, stir, fermentation temperature is 25 ℃-32 ℃;
(5) tunning of gained is drawn, drying, obtain protein feed.
Cellulose decomposes mould and can prepare through the following steps:
(1) bacterial classification with mould activates on activation medium;
(2) nutrient salt solution is added dry water plant powder, nutrient salt solution and dry water plant powder mass ratio are 2: 1-4: 1, and stir the back and sterilized 20-40 minute down at 110 ℃-130 ℃, obtain the mould seed culture medium;
(3) bacterial classification of step (1) gained is inserted the mould seed culture medium of gained, under 25 ℃ of-32 ℃ of conditions, cultivate and obtained cellulose in 48-72 hour and decompose mould.
Saccharomycete can prepare through the following steps:
(1) saccharomycetic bacterial classification is activated on activation medium;
(2) under 115 ℃-121 ℃, maltose was sterilized 20-30 minute, obtain the saccharomycete bacterium culture medium;
(3) the saccharomycete bacterial classification with step (1) gained inserts the saccharomycete bacterium culture medium, and vibration was cultivated 48-72 hour.
Employed cellulose decomposes mould can be mould by aspergillus niger, healthy and free from worry wood, Trichoderma viride etc. makes; Saccharomycete can be made by candida utili, candida tropicalis, and these bacterial classifications can be buied from DSMZ of Institute of Microorganism, Academia Sinica.
The present invention is according to mould and saccharomycetic mutualism relation principle, utilizes highly effective cellulose to decompose mould the cellulose and the hemicellulose of water plant body decomposed, and generates glucose products; Behind mould effect certain hour, excessive glucose products can produce feedback supression effect to the decomposition of cellulose and hemicellulose; Insert high-efficiency feed fermentation special yeast bacterium again, a large amount of consumption of glucose products generate mycoprotein, thereby improve the product protein content.The present invention utilizes mould and saccharomycetic this mutualism principle exactly, under optimum conditions with mould and saccharomycete Mixed culture, farthest reduce the cellulose and the hemicellulose level of water plant principle, improve its protein content, thereby improve palatability, the content that has additional nutrients promotes economic use value.
3, beneficial effect: the present invention has the following advantages:
(1) the present invention's solid fermentation route, water plant is fully utilized, help to prevent secondary pollution, make sustainable the changing into of lake ecological reparation be possible, and its product is a protein feed, has also realized economic benefit when having improved social benefit;
(2) simple operating steps, the equipment maturation, production cost is lower, is applicable to the production of water plant protein feed;
(3) by mould and saccharomycete mixed culture fermentation, utilize saccharomycete to reduce of the supression effect of middle sugared product to cellulase, significantly improved the protein yield of tunning, the recruitment of crude protein reaches as high as 102.66% in the tunning, and utilizing mould to produce protein feed merely, the recruitment of crude protein is up to 28.73% in the tunning.。
Four, description of drawings
Accompanying drawing is a process flow diagram of the present invention.
Five, the specific embodiment
Embodiment 1: as shown in drawings, the lay eggs method of white feed of the fermenting solid-state aquatic plant by microbe of present embodiment comprises the following steps:
(1) with the eel grass raw material, utilize the air dry mode that a part of eel grass is wherein carried out drying, utilize disintegrating apparatus to pulverize again, another part eel grass is pulverized under dampness;
(2) two kinds of raw materials with gained mix, and in the composite material of gained, the quality of moisture is for mixing 50% of back raw material quality;
(3) according to the quality of raw material after the above-mentioned mixing, stir behind the wheat bran of adding 10% and 1% the urea, make fermentation medium;
(4) fermentation medium is packed in the fermentation reactor, the cellulose of 1% percentage by weight is decomposed mould insert and cultivated 12 hours, ventilate in the incubation and stir;
(5) inoculate the saccharomycete of 1% percentage by weight and cultivating 48 hours, stir under ventilation state, fermentation temperature is 25 ℃;
(6) tunning of gained is drawn drying after, send into the extruder extrusion modling, promptly obtain the protein feed of granulated.
Cellulose decomposes mould and can prepare through the following steps:
(1) bacterial classification with aspergillus niger activates on activation medium;
(2) nutrient salt solution is added dry water plant powder, nutrient salt solution and dry water plant powder mass ratio are 2: 1, stir the back and sterilize 40 minutes down at 130 ℃, obtain black-koji mould seed culture medium;
(3) aspergillus niger strain of step (1) gained is inserted the black-koji mould seed culture medium of gained, under 32 ℃ of conditions, cultivate and obtained cellulose in 72 hours and decompose mould.
Saccharomycete can prepare through the following steps:
(1) bacterial classification with candida utili activates on activation medium;
(2) under 121 ℃, maltose was sterilized 30 minutes, obtain the saccharomycete bacterium culture medium;
(3) bacterial classification with the candida utili of step (1) gained inserts the saccharomycete bacterium culture medium, and vibration was cultivated 48 hours.
Embodiment 2: as shown in drawings, the lay eggs method of white feed of the fermenting solid-state aquatic plant by microbe of present embodiment comprises the following steps:
(1) be raw material with little tooth bog pondweed, utilize the air dry mode that the little tooth bog pondweed of a part is wherein carried out drying, utilize disintegrating apparatus to pulverize again, another part is pulverized under dampness by little tooth bog pondweed;
(2) two kinds of raw materials with gained mix, and in the composite material of gained, the quality of moisture is for mixing 70% of back raw material quality;
(3) according to the quality of raw material after the above-mentioned mixing, stir behind the wheat bran of adding 20% and 2% the urea, make fermentation medium;
(4) fermentation medium is packed in the fermentation reactor, the cellulose of 5% percentage by weight is decomposed mould insert and cultivated 48 hours, ventilate in the incubation and stir;
(5) inoculate the saccharomycete of 5% percentage by weight and cultivating 96 hours, stir under ventilation state, fermentation temperature is 32 ℃;
(6) tunning of gained is drawn drying after, send into the extruder extrusion modling, promptly obtain the albumen of granulated.
Cellulose decomposes mould and can prepare through the following steps:
(1) bacterial classification with aspergillus niger activates on activation medium;
(2) nutrient salt solution is added dry water plant powder, nutrient salt solution and dry water plant powder mass ratio are 4: 1, stir the back and sterilize 20 minutes down at 110 ℃, obtain black-koji mould seed culture medium;
(3) aspergillus niger strain of step (1) gained is inserted the black-koji mould seed culture medium of gained, under 25 ℃ of conditions, cultivate and obtained cellulose in 48 hours and decompose mould.
Saccharomycete can prepare through the following steps:
(1) bacterial classification with candida utili activates on activation medium;
(2) under 115 ℃, maltose was sterilized 20 minutes, obtain the saccharomycete bacterium culture medium;
(3) bacterial classification with the candida utili of step (1) gained inserts the saccharomycete bacterium culture medium, and vibration was cultivated 72 hours.
Embodiment 3: as shown in the figure, the lay eggs method of white feed of the fermenting solid-state aquatic plant by microbe of present embodiment comprises the following steps:
(1) be raw material with the waterweed, utilize the air dry mode that a part of waterweed is wherein carried out drying, utilize disintegrating apparatus to pulverize again, another part waterweed is pulverized under dampness;
(2) two kinds of raw materials with gained mix, and in the composite material of gained, the quality of moisture is for mixing 60% of back raw material quality;
(3) according to the quality of raw material after the above-mentioned mixing, stir behind the wheat bran of adding 15% and 1.5% the urea, make fermentation medium;
(4) fermentation medium is packed in the fermentation reactor, the cellulose of 13% percentage by weight is decomposed mould insert and cultivated 30 hours, ventilate in the incubation and stir;
(5) inoculate the saccharomycete of 3% percentage by weight and cultivating 60 hours, stir under ventilation state, fermentation temperature is 30 ℃;
(6) tunning of gained is drawn drying after, send into the extruder extrusion modling, promptly obtain the protein feed of granulated.
Cellulose decomposes mould and can prepare through the following steps:
(1) bacterial classification with Trichoderma viride activates on activation medium;
(2) nutrient salt solution is added dry water plant powder, nutrient salt solution and dry water plant powder mass ratio are 3: 1, stir the back and sterilize 30 minutes down at 121 ℃, obtain the trichoderma viride seed culture medium;
(3) the Trichoderma viride bacterial classification of step (1) gained is inserted the trichoderma viride seed culture medium of gained, under 28 conditions, cultivate and obtained cellulose in 60 hours and decompose mould.
Saccharomycete can prepare through the following steps:
(1) bacterial classification with the candida tropicalis bacterium activates on activation medium;
(2) under 118 ℃, maltose was sterilized 25 minutes, obtain the candida tropicalis bacterium culture medium;
(3) with step 1. the saccharomycete bacterial classification of gained insert candida tropicalis bacterium bacterium culture medium, vibration was cultivated 48-72 hour.
Claims (5)
1, a kind of method of fermenting solid-state aquatic plant by microbe to produce protein feedstuff is characterized in that: this method may further comprise the steps:
(1) be raw material with the water plant, utilize disintegrating apparatus to pulverize, after the raw material pulverizing, the quality of moisture content is the 50%-70% of raw material quality;
(2) press the quality of above-mentioned raw materials, stir after adding the urea of the wheat bran of 10%-20% and 1%-5%, make fermentation medium;
(3) fermentation medium is packed in the fermentation reactor, the cellulose of the 1%-5% of fermentation medium quality is decomposed mould insert and cultivated 12-48 hour, ventilate in the incubation and stir; Fermentation temperature is 25 ℃-32 ℃;
(4) inoculate the fermentation medium quality 1%-5% saccharomycete and cultivated 48 hours to 96 hours, under ventilation state, stir, fermentation temperature is 25 ℃-32 ℃;
(5) tunning of gained is drawn, drying, obtain protein feed.
2, the method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff according to claim 1 is characterized in that: in step (3), cellulose decomposes mould and prepares through the following steps:
(1) bacterial classification with mould activates on activation medium;
(2) nutrient salt solution is added dry water plant powder, nutrient salt solution and dry water plant powder mass ratio are 2: 1-4: 1, and stir the back and sterilized 20-40 minute down at 110 ℃-130 ℃, obtain the mould seed culture medium;
(3) bacterial classification of step (1) gained is inserted the mould seed culture medium of gained, under 25 ℃ of-32 ℃ of conditions, cultivated 48-72 hour, obtain cellulose and decompose mould.
3, the method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff according to claim 1 is characterized in that: in step (4), saccharomycete prepares through the following steps:
(1) saccharomycetic bacterial classification is activated on activation medium;
(2) under 115 ℃-121 ℃, maltose was sterilized 20-30 minute, obtain the saccharomycete bacterium culture medium;
(3) the saccharomycete bacterial classification with step (1) gained inserts the saccharomycete bacterium culture medium, and vibration was cultivated 48-72 hour.
4, the method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff according to claim 1 and 2 is characterized in that: it is aspergillus niger that described cellulose decomposes mould.
5, according to the method for claim 1 or 3 described fermenting solid-state aquatic plant by microbe to produce protein feedstuff, it is characterized in that: described saccharomycete is a candida utili.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100386135A CN100379355C (en) | 2005-03-30 | 2005-03-30 | Method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff |
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| CNB2005100386135A CN100379355C (en) | 2005-03-30 | 2005-03-30 | Method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff |
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| CN100379355C CN100379355C (en) | 2008-04-09 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028096A (en) * | 2009-10-06 | 2011-04-27 | 张宗舟 | Production method of tray liquid high-density bacterial proteins |
| CN104131039A (en) * | 2014-08-06 | 2014-11-05 | 南京大学 | Method for producing organic acid by anaerobic fermentation of aquatic plant |
| CN105211618A (en) * | 2014-05-28 | 2016-01-06 | 吴光平 | A kind of preparation method of dry water plant powder |
| CN110973352A (en) * | 2019-12-30 | 2020-04-10 | 上海太和水环境科技发展股份有限公司 | Method and equipment for recycling aquatic weeds |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3806600A (en) * | 1970-09-28 | 1974-04-23 | Grace W R & Co | Ammonium and potassium isobutyrates as pathogen growth inhibitors and growth stimulators |
| JPS51130583A (en) * | 1975-05-07 | 1976-11-12 | Mitsubishi Heavy Ind Ltd | Process for producing scp or alcohol from organic wastes, espectially kitchen wastes |
| US4243686A (en) * | 1979-05-30 | 1981-01-06 | The United States Of America As Represented By The Secretary Of Agriculture | Process for improving the palatability of straw for animal feed |
| CN1179898A (en) * | 1997-09-26 | 1998-04-29 | 潘祥泉 | Water hyacinth fermentation powder and its producing method and its application in fodder |
-
2005
- 2005-03-30 CN CNB2005100386135A patent/CN100379355C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028096A (en) * | 2009-10-06 | 2011-04-27 | 张宗舟 | Production method of tray liquid high-density bacterial proteins |
| CN105211618A (en) * | 2014-05-28 | 2016-01-06 | 吴光平 | A kind of preparation method of dry water plant powder |
| CN104131039A (en) * | 2014-08-06 | 2014-11-05 | 南京大学 | Method for producing organic acid by anaerobic fermentation of aquatic plant |
| CN104131039B (en) * | 2014-08-06 | 2017-06-23 | 南京大学 | A kind of method that water plant anaerobic fermentation produces organic acid |
| CN110973352A (en) * | 2019-12-30 | 2020-04-10 | 上海太和水环境科技发展股份有限公司 | Method and equipment for recycling aquatic weeds |
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| Publication number | Publication date |
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| CN100379355C (en) | 2008-04-09 |
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Assignee: Jiangsu Jiangda Ecological Technology Co., Ltd. Assignor: Nanjing University Contract record no.: 2011320010028 Denomination of invention: Method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff Granted publication date: 20080409 License type: Exclusive License Open date: 20050907 Record date: 20110329 |
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