CN102160637A - Composition with auxiliary protection effect on chemical hepatic injury and alcoholic hepatic injury and health care food thereof - Google Patents
Composition with auxiliary protection effect on chemical hepatic injury and alcoholic hepatic injury and health care food thereof Download PDFInfo
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- CN102160637A CN102160637A CN2011100031345A CN201110003134A CN102160637A CN 102160637 A CN102160637 A CN 102160637A CN 2011100031345 A CN2011100031345 A CN 2011100031345A CN 201110003134 A CN201110003134 A CN 201110003134A CN 102160637 A CN102160637 A CN 102160637A
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- silymarin
- turmeric
- auxiliary protection
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- root extract
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a composition with auxiliary protection effect on chemical hepatic injury and alcoholic hepatic injury and health care food thereof. The composition or the health care food comprises the following components in percentage by weight: 8-15% of silymarin, 10-20% of wolfberry fruit extract, 15-30% of licorice root extract, 15-20% of kudzuvine root extract, 15-20% of wolfberry fruit extract, and 12-25% of turmeric extract. The health care food provided by the invention has the comprehensive functions of silymarin, wolfberry fruit extract, licorice root extract, kudzuvine root extract, wolfberry fruit extract and turmeric extract, and the components take synergistic action, so as to greatly improve the administration effect of a single component. Functional tests prove that the health care food has auxiliary protection effect on chemical hepatic injury and alcoholic hepatic injury. The preparation method of the health care food is simple, which meets the need of large-scale industrial production.
Description
Technical field
The present invention relates to a kind of health food, specifically, relate to a kind of composition and health food thereof that chemistry and alcoholic liver injury is had auxiliary protection function.
Background technology
Current society has entered fast-developing cyberage, and Human's production environment and living environment are maked rapid progress, and bring the technological means of change to human society, when conquering nature, are also directly threatening human existence.
Liver is again important detoxifcation organ as a metabolism center of body, and known its function is kind more than thousand nearly.The chemical substance that enters in the human body from various approach finally all will transform in liver or store, and causes hepatic injury especially easily.Drink, residual agricultural chemicals, industry and environmental pollution, drug toxicity is the major reason that causes chemical damage in the food.
The lab index of chemical damage is mainly the situations such as rising of serum transaminase and bilirubinic rising, seralbumin reduction, globulin rising, prolonged prothrombin, serum alkaline phosphatase and glutamyl transferase, finally causes necrosis of liver cells, steatosis, liver fibrosis, cirrhosis and canceration in various degree.
Alcohol absorbs rapidly through gastrointestinal tract mucosa and enters blood, becomes the very strong acetaldehyde of toxicity through liver metabolism.Acetaldehyde enters blood, is transformed into superoxides by xanthine oxidase, causes lipid peroxidation, destroys cell membrane, causes hepatic injury.Alcohol can also induce liver P450 enzymatic activity to increase, and (reactive oxygen species ROS), causes lipid peroxidation, destroys hepatocellular 26S Proteasome Structure and Function to produce a large amount of active oxygens in the liver metabolism process.
The Western medicine that a lot of liver protectings, treatment hepatitis are also arranged at present, but these medicines often when protecting liver its in vivo metabolic process generate the material of infringement liver again, be not suitable for taking for a long time, be difficult to satisfy the health care needs of body health of people; And utilize traditional traditional Chinese medicine to protect liver in the existing long history of China, but drawbacks such as inconvenience, dosage and difficult quality control are taken in the existence of traditional traditional Chinese herbal decoction.Therefore utilize modern biotechnology; discover that tradition protects the mechanism of action of liver Chinese medicine and medicine-food two-purpose medicinal material; in conjunction with modern preparation technique, what research and development were fit to take for a long time has the health food of auxiliary protection function to have realistic meaning to chemical damage and alcoholic liver injury.
Silymarin can also be applied to cirrhosis as a kind of medicine for the treatment of acute and chronic hepatitis, fatty liver, toxic liver injury, the hepatic injury that alcohol, medicine and other factors cause, protection liver.What up to now, also do not have a State Food and Drug Administration approval makes the health food of main raw material by silymarin.
Summary of the invention
Technical problem to be solved by this invention is to provide composition and the health food thereof that chemistry and alcoholic liver injury is had auxiliary protection function.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of composition that chemical damage and alcoholic liver injury is had auxiliary protection function; described composition is made up of silymarin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, and its percentage by weight is silymarin 8-15%, Ganodenna Lucidum P.E 10-20%, licorice 15-30%, kudzu root extract 15-20%, Fructus lycii P.E 15-20%, Turmeric P.E 12-25%.
A kind of health food, described health food comprise the described composition that chemical damage and alcoholic liver injury is had auxiliary protection function.
Preferably; chemical damage and alcoholic liver injury are had the health food of auxiliary protection function, and its percentage by weight is silymarin 12%, Ganodenna Lucidum P.E 15%, licorice 20%, kudzu root extract 18%, Fructus lycii P.E 15%, Turmeric P.E 20%.
Preferably; chemical damage and alcoholic liver injury are had the health food of auxiliary protection function, and its percentage by weight is silymarin 10%, Ganodenna Lucidum P.E 15%, licorice 20%, kudzu root extract 15%, Fructus lycii P.E 15%, Turmeric P.E 25%.
Preferably; chemical damage and alcoholic liver injury are had the health food of auxiliary protection function, and its percentage by weight is silymarin 15%, Ganodenna Lucidum P.E 10%, licorice 15%, kudzu root extract 20%, Fructus lycii P.E 20%, Turmeric P.E 20%.
Preferably; above-mentioned have the health food of auxiliary protection function to chemical damage and alcoholic liver injury, and its percentage by weight is silymarin 8%, Ganodenna Lucidum P.E 20%, licorice 30%, kudzu root extract 15%, Fructus lycii P.E 15%, Turmeric P.E 12%.
Above-mentioned have the preparation method of the health food of auxiliary protection function to chemical damage and alcoholic liver injury, and concrete preparation process is as follows:
Get the ganoderma lucidum fruitbody decontamination of band spore, pulverize, add 20 times of water gagings and soak, 95 ℃ of lixiviates, each 3h, continuous 3 times.Merge three times leaching liquor, 60 ℃ of vacuum concentrate, and are concentrated into proportion and are about 1.1.Concentrate adds 95% precipitation with alcohol, the 10～12h of 3 times of volumes, the centrifugal supernatant of removing.Freezing, the vacuum drying of sediment promptly gets Ganodenna Lucidum P.E.
Extracting liquorice cleans, dry, pulverizing back refluxing extraction 3 times (adding 5,4,4 times of water gagings respectively), and each 2h merges extract, is concentrated into 1/5 of original volume, filters, and the filtrate continuation concentrates, and 140～160 ℃ of spray-dryings of concentrate promptly get licorice.
Add 5 times of water gaging refluxing extraction 2 times respectively after getting root of kudzu vine cleaning, drying, pulverizing, each 2h filters, and merges secondary filtrate, centrifugation, filtrate after centrifugal is concentrated, and 95% ethanol that adds 3 times of volumes again stirs evenly, and leaves standstill, centrifugal, decompression filtrate recycling ethanol after centrifugal, 140～160 ℃ of spray-dryings of concentrate promptly get kudzu root extract.
Get the fruit of Chinese wolfberry clean, dry, add 5 times of water gagings after pulverizing respectively refluxing extraction 3 times, each 2h, filter, merge three times filtrate, 60 ℃ of vacuum concentrate, and 95% ethanol that concentrate adds 3 times of volumes stirs evenly, leave standstill, decompression filtrate recycling ethanol after centrifugal, 140～160 ℃ of spray-dryings of concentrate promptly get Fructus lycii P.E.
Cross 40 mesh sieves after getting turmeric cleaning, drying, pulverizing, heating and refluxing extraction is 3 times in 80% ethanol, 3 times ethanol consumption is 6 times of crude drug weight, each 2h of extraction time, merge extract, filtration, 60 ℃ of decompression recycling ethanols of filtrate, 140～160 ℃ of spray-dryings of concentrate promptly get Turmeric P.E.
Water intaking flies silibin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E and sieves respectively, and it is promptly encapsulated to mix the back after with each feed proportioning by formula ratio.
That uses that above-mentioned preparation method makes has the health food of auxiliary protection function to chemical damage and alcoholic liver injury, takes every day 3 times, takes 3 (0.25g/ grains) at every turn.
Silymarin is a kind of active extract that extracts from the milk thistle fruit, is made up of four isomers of chromanone group, and what wherein play pharmacological action is legalon.At first, legalon can be integrated in the liver plasma membrane, competitive in conjunction with the specific receptor in the liver plasma membrane, play stable liver plasma membrane, the blocking-up cell membrane can be kept the activity of physiological polyphenoils such as glutathione and superoxide dismutase by such receptor-mediated picked-up to the virose material of liver; Secondly, legalon can combine and make its activation with ER after entering liver cell, but the activity of the ER enhance hepatocyte nuclear RNA polymerase I of activation, rRNA is transcribed to be increased, cause endochylema internal ribosome number to increase, thereby promote the biosynthesis of functional protein (enzyme) and structural albumen, the mode with biofeedback promotes the synthetic of cell DNA again, thereby helps hepatocellular reparation and regeneration; In addition, also can remove free radical, alleviate the hepatocellular injury and the tight inflammation of hepatic tissue of its mediation, stop its triggering to liver fibrosis, by downward modulation type i collagen protein mRNA content, suppress fibroplasia and downward modulation metalloprotease tissue inhibiting factor one 1mRNA, thereby suppress the synthetic of metalloprotease tissue inhibiting factor 1, promote that collagen goes down, play indirect and direct anti-fibrosis effect.
Glossy ganoderma is as traditional invigorant, can obviously reduce the rising of the serum glutamic pyruvic transminase that carbon tetrachloride, thioacetamide cause, can also significantly alleviate accumulating of mouse liver fat that carbon tetrachloride, thioacetamide cause, suppress the lipid peroxidation injury of ethanol to liver, promote the liver cell repair, strengthen the power of regeneration of liver, strengthen liver detoxification function, also can to hepatitis the supplemental treatment effect be arranged by the function of immune system that strengthens body.
Radix Glycyrrhizae mainly contains glycyrrhizic acid and flavone compound.Flavonoids of Glycyrrhiza can significantly be protected alcohol induced liver MDA (MDA) increase and the exhaustion of reduced glutathione (GSH).Also can protect the damage of the ultrastructure of hepatic cell due to the ethanol; Glycyrrhizic acid can prevent carbon tetrachloride and the liver fibrosis of N-nitrosodimethylamine induced mice; Glycyrrhizic acid also can suppress the Cytochrome P450 activity of isoenzyme of " increasing poison ", reduces poisonous substance and carcinogenic metabolism activation, can significantly induce II phase enzymatic activity again, accelerates poisonous substance and carcinogenic drainage.
The root of kudzu vine just is used for relieving alcoholism since ancient times, and the modern pharmacological research proof root of kudzu vine has the drunk effect of tangible prevention; The effectively liver lipid peroxidation infringement that causes of antagonism alcohol, drink kudzu root extract effectively the antagonism drink caused liver MDA of ethanol (MDA) level improve; Pueraria Flavonid can significantly be induced the effect of P 450; The root of kudzu vine also can resist the apolipoprotein effect of reduction serum and the rising cholesterol and the triglycerides effect of ethanol.
The fruit of Chinese wolfberry has good hepatoprotective effect.LBP-X can obviously suppress the rising of hepatic injury mouse ALT due to the CCl4, increases liver glycogen content, and can obviously reduce MDA content in the liver, and the lobuli hepatis damage field is dwindled, and fat drips minimizing in the liver cell, and nucleus increases, and RNA and kernel increase; Matrimony vine also has certain effect to the inhibition that diethylnitrosamine brings out rat liver cancer.
Main active curcumin in the turmeric is the potent inhibitor of lipid peroxidation, can suppress the generation of ROS in the hepatic tissue, can remove the oxygen radical that produces in the oxidizing process again, strengthen the liver oxidation resistance, alleviate oxidative stress, thereby play the hepatocellular effect of protection; Curcumin can also improve fatty acid metabolism, reduces the deposition of fat at liver; The release of the activation that curcumin also can be by suppressing nuclear factor KB (NF.KB) and the cell factor of mediation thereof suppresses effects such as the synthetic and HSCs activation of collagen, thereby alleviates liver inflammation and Fibrotic development.
The described health food that chemical damage and alcoholic liver injury is had auxiliary protection function; comprehensive function with silymarin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E; and act synergistically mutually; promoted the effect of taking of one-component greatly; prove through function test; chemical damage and alcoholic liver injury are had auxiliary protection function, and its preparation method is simple, is fit to requirements of large-scale industrial production.
The specific embodiment
Below in conjunction with specific embodiment technical scheme of the present invention is described further, wherein, related embodiment should not be regarded as the restriction to protection scope of the present invention.
Embodiment 1
Get the ganoderma lucidum fruitbody decontamination of band spore, pulverize, add 20 times of water gagings and soak, 95 ℃ of lixiviates, each 3h, continuous 3 times.Merge three times leaching liquor, 60 ℃ of vacuum concentrate, and are concentrated into proportion and are about 1.1.Concentrate adds 95% precipitation with alcohol, the 10～12h of 3 times of volumes, the centrifugal supernatant of removing.Freezing, the vacuum drying of sediment promptly gets Ganodenna Lucidum P.E.
Extracting liquorice cleans, dry, pulverizing back refluxing extraction 3 times (adding 5,4,4 times of water gagings respectively), and each 2h merges extract, is concentrated into 1/5 of original volume, filters, and the filtrate continuation concentrates, and 140～160 ℃ of spray-dryings of concentrate promptly get licorice.
Add 5 times of water gaging refluxing extraction 2 times respectively after getting root of kudzu vine cleaning, drying, pulverizing, each 2h filters, and merges secondary filtrate, centrifugation, filtrate after centrifugal is concentrated, and 95% ethanol that adds 3 times of volumes again stirs evenly, and leaves standstill, centrifugal, decompression filtrate recycling ethanol after centrifugal, 140～160 ℃ of spray-dryings of concentrate promptly get kudzu root extract.
Get the fruit of Chinese wolfberry clean, dry, add 5 times of water gagings after pulverizing respectively refluxing extraction 3 times, each 2h, filter, merge three times filtrate, 60 ℃ of vacuum concentrate, and 95% ethanol that concentrate adds 3 times of volumes stirs evenly, leave standstill, decompression filtrate recycling ethanol after centrifugal, 140～160 ℃ of spray-dryings of concentrate promptly get Fructus lycii P.E.
Cross 40 mesh sieves after getting turmeric cleaning, drying, pulverizing, heating and refluxing extraction is 3 times in 80% ethanol, 3 times ethanol consumption is 6 times of crude drug weight, each 2h of extraction time, merge extract, filtration, 60 ℃ of decompression recycling ethanols of filtrate, 140～160 ℃ of spray-dryings of concentrate promptly get Turmeric P.E.
Water intaking flies silibin 6kg, Ganodenna Lucidum P.E 7.5kg, licorice 10kg, kudzu root extract 9kg, Fructus lycii P.E 7.5kg, Turmeric P.E 10kg sieve respectively, mixes the back and divides encapsulated 200000.
Embodiment 2
The health food that chemical damage and alcoholic liver injury is had auxiliary protection function; be made up of silymarin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, its percentage by weight is silymarin 10%, Ganodenna Lucidum P.E 15%, licorice 20%, kudzu root extract 15%, Fructus lycii P.E 15%, Turmeric P.E 25%.The preparation method is with embodiment 1.
Embodiment 3
The health food that chemical damage and alcoholic liver injury is had auxiliary protection function; be made up of silymarin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, its percentage by weight is silymarin 15%, Ganodenna Lucidum P.E 10%, licorice 15%, kudzu root extract 20%, Fructus lycii P.E 20%, Turmeric P.E 20%.The preparation method is with embodiment 1.
Embodiment 4
The health food that chemical damage and alcoholic liver injury is had auxiliary protection function; be made up of silymarin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, its percentage by weight is silymarin 8%, Ganodenna Lucidum P.E 20%, licorice 30%, kudzu root extract 15%, Fructus lycii P.E 15%, Turmeric P.E 12%.The preparation method is with embodiment 1.
Embodiment 5
Safety evaluatio
Embodiment 1 carries out the test of security toxicological evaluation by the GB15193-1994 pertinent regulations, and method and result are as follows:
Acute toxicity test: take by weighing sample 1000mg, adding distil water fully is configured to even suspension behind the mixing, as given the test agent to 20ml.Kunming mouse fasting (can't help water) is after 16 hours, select each 10 of male and female mouse, body weight is between the 18-22 gram, divide and be put in two mouse cages, difference with body weight between the sex mouse is no more than 3g, adopting a per os to irritate the stomach mode given the test agent contaminates to animal used as test, irritates gastric capacity and press the 0.4ml/20g batheroom scale.Observe general state, changes of weight, poisoning symptom and death condition etc. to animal in the week behind the sample.The result: each treated animal of duration of test is movable normal, and the hair color glossiness is good, does not see any poisoning symptom and death; Female mice LD
50＞10000mg/kg, male mice LD
50＞10000mg/kg.Conclusion: according to the classification of acute toxicity half lethal dose, true border nontoxic level material.
Micronucleus test: take by weighing sample 5000,2500,1250mg, adding distil water is a given the test agent behind the mixing fully to 20ml respectively.Get Kunming mouse, body weight 25-30g, animal is divided into 5 groups at random, every group 10, male and female half and half, respectively as three dosage groups of sample and distilled water negative control group, endoxan positive controls, adopt 30 hours twice administration by gavage, will prepare the variable concentrations sample and press the 0.4ml/20g body weight, respectively each treated animal is irritated stomach, 6 hours execution animals after irritating stomach for the second time, get femur bone marrow and add the calf serum smear, carry out Giemsa dyeing, microscopy is observed, the micronucleus number of 1000 polychromatic erythrocytes of every mouse counting calculates microkernel incidence.Result: feminine gender.
Sperm malformation test: take by weighing sample 5000,2500,1250mg, adding distil water is a given the test agent behind the mixing fully to 20ml respectively.Select 25 Kunming mouses, male and female half and half, body weight is between the 25-35 gram, be divided into 5 groups, as three dosage groups of sample and distilled water negative control group, endoxan positive controls, will prepare the variable concentrations sample and press the 0.4ml/20g body weight respectively, respectively each treated animal is irritated stomach, once a day, continuous 5 days, after the 35th day, put to death animal, get epididymis filtrate smear, the dyeing of 2% Yihong, microscopy is observed the lopsided number of 1000 sperms of every mouse, calculates the teratospermia incidence.Result: feminine gender.
Salmonella reversion test: take by weighing sample 1000mg, add sterile distilled water to 20ml, mixing promptly gets high dose group and is subjected to test solution, draws the above-mentioned test solution 5.0,2.0,1.0 that is subjected to, and 0.2ml adds sterile distilled water respectively to the be subjected to test solution of 10ml as following each dosage group.Select TA97, TA98, TA100, TA102 test strain and carry out inducing and preparing of rats'liver S9, test by GB15193-94 dressing plate infiltration method.Result: feminine gender.
30 days feeding trials: select 80 of SD rats, body weight 60-80g, male and female half and half are divided 4 groups, and wherein 3 groups is test group, and 1 group is the blank group.As experimental group product being made feed granules list cage by 10 times, 50 times, 100 times of human dose feeds, free diet, feed after 30 days continuously, by only weighing, get mouse blood and carry out hematology, biochemical analysis, put to death, gross anatomy is observed and is had or not obvious pathology, get internal organs such as liver,kidney,spleen, sexual organ and weigh, calculate dirty body ratio, and these internal organs are carried out histopathological examination.The result shows: the animal used as test growing state is good, hematological examination, and biochemical analysis, main dirty body is histological examination result when compare with control group, all no significant difference.
Embodiment 6
Auxiliary protection function to chemical damage
Carried out having the animal function assessment of auxiliary protection function to test with preparation of the present invention and by " health food check and assessment technique standard " (version in 2003) to chemical damage.
Given the test agent: the embodiment of the invention 1 preparation, silymarin preparation (silymarin content is with the contained silymarin content of embodiment 1 preparation), middle medicine composite preparation (be made up of Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, each constituent content is with embodiment 1 preparation).
Dosage design: be 10 times of preparation human body RD of the present invention, i.e. embodiment 1 preparation group 0.375g/kg, silymarin preparation group 0.045g/kg, middle medicine composite preparation group 0.33g/kg, other establishes liver injury model group and normal control group (giving distilled water).
Sample treatment: get embodiment 1 preparation 0.375g, silymarin preparation 0.045g, middle medicine composite preparation 0.33g adding distil water respectively make into even suspension to 20ml, are test liquid.
Animal used as test: Kunming mouse, 18-22g, male, the cleaning level small white mouse that provides by animal used as test portion of Medical Center of Fudan University.20-24 ℃ of receptacle temperature, relative humidity 40-70%.
Give sample loading mode: irritate stomach, irritating the stomach volume is 0.4ml/20g.
Experimental technique and result: select 50 of healthy mices, be divided into 5 groups at random, 10 every group.Each sample sets is given sample, and control group and model group are drunk distilled water, continuous 30 days.The 30th day each sample sets and model group are contaminated dosage 24mg/kg, lumbar injection (i.p) with CCL4.The 31st day, 5 treated animals are plucked eyeball and got blood, and were centrifugal, get serum.Measure following biochemical indicator: serum glutamic pyruvic transminase (GPT), glutamic-oxalacetic transaminease (GOT), white/globulin ratio (A/G); And get liver and carry out histopathologic examination.Experimental result Spss software statistics.
Sample is as shown in table 1 to the influence of the weight of animals.
Table 1
| Group | Number of animals | Initial body weight | Mid-term body weight | Mid-term body weight |
| The normal control group | 10 | 19±1.0 | 28±1.4 | 35±1.6 |
| The CCL4 model group | 10 | 19±1.0 | 28±1.6 | 35±1.5 |
| Middle medicine composite preparation group | 10 | 19±1.0 | 28±1.1 | 35±1.9 |
| Silymarin preparation group | 10 | 19±1.0 | 28±1.0 | 35±1.9 |
| Embodiment 1 preparation group | 10 | 19±1.0 | 28±1.2 | 35±1.8 |
As seen from the above table, each sample sets body weight is compared with the CCL4 model group, difference that there are no significant.
The biochemistry detection result of sample is as shown in table 2.
Table 2
*: P＜0.05 and CCL
4Model group is (through variance analysis) relatively; (through variance analysis) compared with embodiment 1 preparation group in #:P＜0.05
As seen from the above table, the glutamic-pyruvic transaminase of normal control group, glutamic-oxalacetic transaminease content are starkly lower than CCL
4There is significant difference in model group, and the model establishment is described.The glutamic-pyruvic transaminase of silymarin preparation group and embodiment 1 preparation group, glutamic-oxalacetic transaminease content all obviously reduce, with CCL
4Relatively all there is significant difference in model group; Middle every index of medicine composite preparation group and CCL
4Model group is compared, there was no significant difference; There were significant difference in medicine composite preparation group and silymarin group during the glutamic-pyruvic transaminase of embodiment 1 preparation group, glutamic-oxalacetic transaminease content were starkly lower than.
The pathological examination results of sample is as shown in table 3.
Table 3
*: P＜0.05 and CCL
4Model group is (through variance analysis) relatively; (through variance analysis) compared with embodiment 1 preparation group in #:P＜0.05
By check substantially and the microscopy result as seen, the pathology integration of normal control group is starkly lower than CCL
4There is significant difference in model group, and the model establishment is described.Embodiment 1 preparation group and CCL
4Model group is compared, and the pathology integration obviously descends, and has significant difference; The pathology integration and the CCL of middle medicine composite preparation group and silymarin preparation group
4Model group is compared, there was no significant difference; There were significant difference in medicine composite preparation group and silymarin preparation group during the pathology integration of embodiment 1 preparation group was starkly lower than.
Conclusion: embodiment 1 preparation per os is fed after 30 days continuously, has the protective effect to chemical damage, and its effect is better than middle medicine composite preparation and silymarin preparation.
Embodiment 7
Auxiliary protection function to alcoholic liver injury
Carried out having the animal function assessment of auxiliary protection function to test with preparation of the present invention and by " health food check and assessment technique standard " (version in 2003) to alcoholic liver injury.
Given the test agent: the embodiment of the invention 1 preparation, silymarin preparation (silymarin content is with the contained silymarin content of embodiment 1 preparation), middle medicine composite preparation (be made up of Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, each constituent content is with embodiment 1 preparation).
Dosage design: be 10 times of preparation human body RD of the present invention, i.e. embodiment 1 preparation group 0.375g/kg, silymarin preparation group 0.045g/kg, middle medicine composite preparation group 0.33g/kg, other establishes liver injury model group and normal control group (giving distilled water).
Sample treatment: get embodiment 1 preparation 0.375g, silymarin preparation 0.045g, middle medicine composite preparation 0.33g adding distil water respectively make into even suspension to 20ml, are test liquid.
Animal used as test: Kunming mouse, 18-22g, male, the cleaning level small white mouse that provides by animal used as test portion of Medical Center of Fudan University.20-24 ℃ of receptacle temperature, relative humidity 40-70%.
Give sample loading mode: irritate stomach, irritating the stomach volume is 0.4ml/20g.
Experimental technique and result: select 50 of healthy mices, be divided into 5 groups at random, 10 every group.Each sample sets is given sample, and control group and 50% ethanol liver injury model group give equivalent distilled water, gives sample 30 days continuously.The 30th day each sample sets and model group give 50% ethanol 0.12ml/10g.bw contamination.After the fasting 12 hours, each animal is got liver, makes 10% LH, measures following biochemical indicator respectively: triglycerides (TG), MDA (MDA), reductive glutathione (GSH); And get liver and carry out histopathologic examination.Experimental result Spss software statistics.
Sample is as shown in table 4 to the influence of the weight of animals.
Table 4
| Group | Number of animals | Initial body weight | Mid-term body weight | Mid-term body weight |
| Normal control | 10 | 19±1.0 | 28±1.0 | 37±1.4 |
| Ethanol model | 10 | 19±1.0 | 28±1.2 | 36±1.2 |
| Middle medicine composite preparation | 10 | 19±1.0 | 28±1.5 | 36±1.6 |
| The silymarin preparation | 10 | 19±1.0 | 28±1.3 | 36±1.1 |
| Embodiment 1 preparation | 10 | 19±1.0 | 28±1.0 | 36±1.6 |
As seen from the above table, each sample sets body weight is compared with the ethanol model group, difference that there are no significant.
The biochemistry detection result of sample is as shown in table 5.
Table 5
*: (through variance analysis) compared with the ethanol model group in P＜0.05; (through variance analysis) compared with embodiment 1 preparation group in #:P＜0.05
As seen from the above table, the triglycerides of normal control group, MDA, reductive glutathione are compared with the ethanol model group, all have significant difference, and the model establishment is described.The triglycerides of embodiment 1 preparation group, MDA, reductive glutathione are compared with the ethanol model group, all have significant difference, illustrate that to improve effect remarkable; And the triglycerides of middle medicine composite preparation group, MDA, reductive glutathione are compared difference that there are no significant with the ethanol model group; The triglycerides of silymarin preparation group, reductive glutathione are compared difference that there are no significant with the ethanol model group.
The pathological examination results of sample is as shown in table 6.
Table 6
*: (through variance analysis) compared with the ethanol model group in P＜0.05
By check substantially and the microscopy result as seen, the pathology integration of normal control group is starkly lower than the ethanol model group, has significant difference, and the model establishment is described.Embodiment 1 preparation group is compared with the ethanol model group, and the pathology integration obviously descends, and has significant difference, has illustrated that conspicuousness improves effect; And the pathology integration of middle medicine composite preparation group, silymarin preparation group is compared with the ethanol model group, difference that there are no significant.
Conclusion: embodiment 1 preparation per os is fed after 30 days continuously, has the protective effect to alcoholic liver injury, and its effect is better than middle medicine composite preparation and silymarin preparation.
Claims (7)
1. composition that chemical damage and alcoholic liver injury is had auxiliary protection function; it is characterized in that; described composition is made up of silymarin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E, and its percentage by weight is silymarin 8-15%, Ganodenna Lucidum P.E 10-20%, licorice 15-30%, kudzu root extract 15-20%, Fructus lycii P.E 15-20%, Turmeric P.E 12-25%.
2. the composition that chemical damage and alcoholic liver injury is had auxiliary protection function as claimed in claim 1; it is characterized in that its percentage by weight is silymarin 12%, Ganodenna Lucidum P.E 15%, licorice 20%, kudzu root extract 18%, Fructus lycii P.E 15%, Turmeric P.E 20%.
3. the composition that chemical damage and alcoholic liver injury is had auxiliary protection function as claimed in claim 1; it is characterized in that its percentage by weight is silymarin 10%, Ganodenna Lucidum P.E 15%, licorice 20%, kudzu root extract 15%, Fructus lycii P.E 15%, Turmeric P.E 25%.
4. the composition that chemical damage and alcoholic liver injury is had auxiliary protection function as claimed in claim 1; it is characterized in that its percentage by weight is silymarin 15%, Ganodenna Lucidum P.E 10%, licorice 15%, kudzu root extract 20%, Fructus lycii P.E 20%, Turmeric P.E 20%.
5. the composition that chemical damage and alcoholic liver injury is had auxiliary protection function as claimed in claim 1; it is characterized in that its percentage by weight is silymarin 8%, Ganodenna Lucidum P.E 20%, licorice 30%, kudzu root extract 15%, Fructus lycii P.E 15%, Turmeric P.E 12%.
6. health food, described health food comprise as each described composition that chemical damage and alcoholic liver injury is had auxiliary protection function among the claim 1-5.
7. the preparation method of a health food as claimed in claim 6, it is characterized in that, water intaking flies silibin, Ganodenna Lucidum P.E, licorice, kudzu root extract, Fructus lycii P.E, Turmeric P.E and sieves respectively, mixes after with each feed proportioning by formula ratio.
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| CN102640898A (en) * | 2012-03-30 | 2012-08-22 | 池州市昭明渔港酒店 | Liver-protecting Qiupu crab spawn big steamed meat bun and making method thereof |
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| CN102640947A (en) * | 2012-03-30 | 2012-08-22 | 池州市昭明渔港酒店 | Big mandarin fish crab spawn meat bun for alleviating alcohol and preparation method thereof |
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| CN104383447A (en) * | 2014-12-01 | 2015-03-04 | 天津中新药业集团股份有限公司达仁堂制药厂 | Traditional Chinese medicine composition as well as preparation method and application thereof |
| CN104667175A (en) * | 2015-02-04 | 2015-06-03 | 辽宁中医药大学 | Health care product with function of protecting alcoholic liver injury |
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| CN101856384A (en) * | 2009-04-09 | 2010-10-13 | 李佳柔 | Liver-protecting combination |
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| CN101053642A (en) * | 2006-04-10 | 2007-10-17 | 广州康臣药业有限公司 | Traditional Chinese medicinal composition for treating fatty liver and its preparation method |
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| CN102630976B (en) * | 2012-03-30 | 2013-11-20 | 池州市昭明渔港酒店 | De-alcoholic fish stuffed bun and making method thereof |
| CN102630873A (en) * | 2012-03-30 | 2012-08-15 | 池州市昭明渔港酒店 | Qiupu crab cream meat bun and preparation method thereof |
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| CN102640948A (en) * | 2012-03-30 | 2012-08-22 | 池州市昭明渔港酒店 | Steamed meat dumpling prepared by crab spawn in Qiupu and used for alleviating hangover and protecting liver |
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| CN102630976A (en) * | 2012-03-30 | 2012-08-15 | 池州市昭明渔港酒店 | De-alcoholic fish stuffed bun and making method thereof |
| CN102640898B (en) * | 2012-03-30 | 2013-11-20 | 池州市昭明渔港酒店 | Liver-protecting Qiupu crab spawn big steamed meat bun and making method thereof |
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| CN103230432A (en) * | 2013-05-03 | 2013-08-07 | 江苏江大源生态生物科技有限公司 | Composition with function of auxiliarily protecting chemical hepatic injury and preparation method of capsule preparation thereof |
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| CN105267291A (en) * | 2014-06-04 | 2016-01-27 | 湘北威尔曼制药股份有限公司 | Compound composition for treating or protecting liver injury |
| CN105267291B (en) * | 2014-06-04 | 2019-12-27 | 湘北威尔曼制药股份有限公司 | Compound composition for treating or protecting liver injury |
| CN104383447B (en) * | 2014-12-01 | 2018-02-16 | 天津中新药业集团股份有限公司达仁堂制药厂 | A kind of Traditional Chinese medicine composition and preparation method and application |
| CN104383447A (en) * | 2014-12-01 | 2015-03-04 | 天津中新药业集团股份有限公司达仁堂制药厂 | Traditional Chinese medicine composition as well as preparation method and application thereof |
| CN105724875A (en) * | 2014-12-08 | 2016-07-06 | 恩施盛硒生物工程有限公司 | Selenium-rich fruit-and-vegetable essence slice with liver protecting function |
| CN104667175A (en) * | 2015-02-04 | 2015-06-03 | 辽宁中医药大学 | Health care product with function of protecting alcoholic liver injury |
| CN104689251A (en) * | 2015-03-26 | 2015-06-10 | 江苏康缘药业股份有限公司 | Traditional Chinese medicine composition alleviating chemical liver injury and preparation method and Chinese materia medica preparation thereof |
| CN106879895A (en) * | 2015-12-15 | 2017-06-23 | 东莞市永益食品有限公司 | Application of the honey matrimony vine tomato juice in the health food for the treatment of or preventing alcoholic liver injury is prepared |
| CN106511757A (en) * | 2016-11-10 | 2017-03-22 | 苏州慧宁堂生物科技有限公司 | Silymarin worm grass lozenge and preparing method |
| CN106721699A (en) * | 2016-11-30 | 2017-05-31 | 江苏食品药品职业技术学院 | A kind of sobering-up beverage and preparation method thereof |
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| CN108904752A (en) * | 2018-07-30 | 2018-11-30 | 湖南科技学院 | A kind of chemical damage therapeutic agent and preparation method thereof |
| CN108835638A (en) * | 2018-08-01 | 2018-11-20 | 黑龙江仁合堂药业有限责任公司 | A kind of Soboring-up liver-protecting improves the food and preparation method thereof of immunity |
| CN112535716A (en) * | 2019-09-20 | 2021-03-23 | 江阴持一堂医药科技有限公司 | Granules for treating alcoholic liver injury and preparation method and application thereof |
| CN112535716B (en) * | 2019-09-20 | 2022-04-15 | 江阴持一堂医药科技有限公司 | Granules for treating alcoholic liver injury and preparation method and application thereof |
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| CN115737777B (en) * | 2022-12-19 | 2023-12-05 | 河南中医药大学 | Compound anti-alcohol traditional Chinese medicine composition and preparation method thereof |
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Effective date of registration: 20231130 Address after: 200233, Room 504, 5th Floor, Building 86, No. 700 Yishan Road, Xuhui District, Shanghai Patentee after: Shanghai Kangpeijian Industrial Co.,Ltd. Address before: Room 2608, No.1 Dapu Road, Luwan District, Shanghai, 200023 Patentee before: Shanghai Huangjindadang Bio-tech Co.,Ltd. Patentee before: SHANGHAI GIANT LIFETECH Co.,Ltd. |