CN102146000A - Method for preparing stevia rebaudiana biological growth promoting agent - Google Patents
Method for preparing stevia rebaudiana biological growth promoting agent Download PDFInfo
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- CN102146000A CN102146000A CN201010108114XA CN201010108114A CN102146000A CN 102146000 A CN102146000 A CN 102146000A CN 201010108114X A CN201010108114X A CN 201010108114XA CN 201010108114 A CN201010108114 A CN 201010108114A CN 102146000 A CN102146000 A CN 102146000A
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- biological growth
- stevia rebaudiana
- promoting agent
- growth promoting
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Abstract
The invention discloses a method for preparing a stevia rebaudiana biological growth promoting agent and belongs to the field of agriculture and comprehensive utilization. In the preparation method, the stevia rebaudiana biological growth promoting agent is prepared by taking stevia rebaudiana waste residues and stevia rebaudiana stems obtained after stevia is extracted as raw materials and adding inorganic salt with trace elements and microbial composite strains for carrying out fermentation. The stevia rebaudiana biological growth promoting agent prepared by the method can be used as a fertilizer and a feed additive. When the stevia rebaudiana biological growth promoting agent is used as the fertilizer, the yield of crops and sweetness can be promoted and soil can be improved. When the stevia rebaudiana biological growth promoting agent is used as the feed additive, the quality and the yield of animal meat can be promoted and the antiviral ability of animals is improved. The stevia rebaudiana biological growth promoting agent has the characteristics of high efficiency, low cost, no toxicity, no residues, environmental protection and the like. The method for preparing the stevia rebaudiana biological growth promoting agent has high application value.
Description
Technical field
The present invention relates to that a kind of the leaf waste residue of sweet Stevia was the technology of preparing that raw material is produced the biological growth agent behind the stevioside to extract, belong to agricultural and comprehensive utilization field.
Background technology
Sweet Stevia is mainly used in industry and extracts stevioside at present, and the leaf waste residue after the extraction can not effectively utilize, thereby causes the pollution of the wasting of resources and environment.Show after deliberation, extracted in the leaf waste residue of stevioside and still contain more vegetable-protein, fiber and trace element, be converted into soil ulmin in placing into the soil, and make the part inorganic salt be converted into organic salt, promote the absorption of plant; In addition, this waste residue is given and animal as fodder additives because of having sweet taste, can strengthen the feed palatability, the animal fast gaining.Based on foregoing, the present invention utilizes the microbial composite bacteria kind that sweet Stevia leaf waste residue and the leaf stalk that extracts pre-treatment are fermented, and adds and mix addition material and make the agent of sweet Stevia biological growth.Studies confirm that through effect it can significantly promote garlic, corn yield, promote sugarcane, apple sugar degree and improve its mouthfeel.Soil with organic matter, N-P-K content all have lifting.Milk cow there is appetite stimulator, improves digestive function, promotes growth, increases milk yield, reduces the effect of somatic number, can increase per day weight, lean ratio and part indispensable amino acid to pork pig, improve feedstuff-meat ratio, can also significantly promote resistance against diseases and the survival rate of piglet.
Summary of the invention
Technical assignment of the present invention is the problem that exists in the present sweet Stevia comprehensive utilization, and the preparation method of a kind of sweet Stevia biological growth agent is provided.Preparation technology is simple for this method, and the sweet Stevia biological growth agent that is prepared by this method has all advantages of bio-feritlizer, and can solve the handling problem of present sweet Stevia leaf waste residue and leaf stalk.
The objective of the invention is to realize in the following manner, utilize the microbial composite bacteria kind to ferment in sweet Stevia leaf waste residue and leaf stalk, and add the mixing addition material.
Sweet Stevia biological growth agent preparation method of the present invention may further comprise the steps:
1) gets leaf waste residue and stalk separated pulverizing after sweet Stevia is extracted, granular size 10～20 orders.Mix the powder of leaf and stalk, the powder of stalk gets 10～90%, and uniform mixing is kept moisture content.Raw material is put into digester, adds water boiling 1～5h, 50～100 ℃ of temperature, and liquid enters the storage tank cooling after the boiling.
2) enter concentration tank by storage tank, carry out evaporation concentration, concentrated solution transfers to pH value about 7 with unslaked lime, adds dregs of beans, urea and contains the inorganic salt etc. of elements such as selenium, germanium, zinc, boron.Drop into 0.1～1.0% microbial composite bacteria kind, guarantee the aeration condition of fermentor tank.Guarantee maturation jar air-proof condition 2～10 months.
3) quantitative package: supernatant liquor mixes in the feed and uses the biological growth agent as animal, and precipitation and suspension are used the biological growth agent as plant.
The preparation method of sweet Stevia biological growth of the present invention agent compared with prior art has following outstanding characteristics:
(1) adopts microbial composite bacteria kind and mixing addition material, guaranteed that the fertilizer after the maturation has efficient quick-acting effect; Soil ulmin and above addition material that the sweet Stevia waste residue forms are had an effect, and make to be difficult to absorbed inorganic salt and to become easy absorbed organic salt, reach and improve soil, keep long lasting effect.
(2) processing, the environment protection for sweet Stevia leaf waste residue and leaf stalk provides effective way.
(3) by measuring the quality that outward appearance, pH, total solid mensuration, total sugar content, total nitrogen content, content of elements guarantee the agent of sweet Stevia biological growth.
Embodiment
Below in conjunction with embodiment this explanation is described in more details, but does not limit the present invention in any form.
Embodiment 1:
Raw material: sweet Stevia leaf waste residue 10kg, stalk 15kg, unslaked lime, the microbial composite bacteria kind (is made up of beneficial microorganisms such as the microorganism of vinelandii, phosphate-solubilizing bacteria, potassium bacterium, photosynthetic bacterium, actinomycetes, product agricultural antibiotic and yeast, genus bacillus, bifidus bacillus, milk-acid bacteria, clostridium butyricums, useful viable count is greater than 2,000,000,000/gram), inorganic salt (Sodium Selenite 26.3g, germanium dioxide 4.3g, Zinc vitriol 13.2g, borax 52.0g), urea 1kg makes 30kg;
Step:
1) get leaf waste residue and stalk separated pulverizing after sweet Stevia is extracted, granular size 30～50 orders mix the powder of leaf and stalk, keep moisture content.Raw material is put into digester, adds water boiling 2h, 70 ℃ of temperature, and liquid enters the storage tank cooling after the boiling.
2) enter concentration tank by storage tank, carry out evaporation concentration, concentrated solution transfers to pH value about 7 with unslaked lime, adds dregs of beans, urea and contains the inorganic salt etc. of elements such as selenium, germanium, zinc, boron.。Drop into 0.2% microbial composite bacteria kind, guarantee the aeration condition of fermentor tank, isolated insect.Guarantee maturation jar air-proof condition 6 months.
3) quantitative package: supernatant liquor mixes in the feed and uses the biological growth agent as animal, and precipitation and suspension are used the biological growth agent as plant.
Embodiment 2:
Sweet Stevia biological growth agent total solid is measured
1) suspension and supernatant liquor adopt ISO 2173-78 " the mensuration compound microcapsule of soluble solid in fruits and vegetables and the goods thereof ".With the abundant mixing of sample, extrude filtrate with four layers of gauze, discard initial several, collect filtrate.
2) dip in glass stick and get 2～3 of test solutions, drip in refractometer prism facets central authorities, the rapid closing prism leaves standstill 1min, makes test solution evenly not have bubble, and is full of the visual field.Alignment light source is observed objective lens by eyepiece.Regulate alidade, make the visual field be divided into two ones of light and shades, the rotary fine adjustment spiral makes terminator clear again, and makes its line of delimitation just on the right-angled intersection point of objective lens.Read percentage ratio or refractive index in the eyepiece visual field, and record prism temperature.Animal is＜7.5% with biological growth agent (liquid) total solid, and plant is＞65.0% with biological growth agent (suspension or semi-fluid) total solid.
Claims (4)
1. the preparation method of sweet Stevia biological growth agent is characterized in that may further comprise the steps:
1) gets leaf waste residue and stalk separated pulverizing after sweet Stevia is extracted stevioside, granular size 10～20 orders.Mix the powder of leaf and stalk, the powder of stalk gets 10～90%, uniform mixing.Put into digester, add water boiling 1～5h, 50～100 ℃ of temperature, liquid enters the storage tank cooling after the boiling.
2) enter concentration tank by storage tank, concentrate, concentrated solution transfers to pH value about 7 with unslaked lime, and adding contains the inorganic salt of elements such as selenium, zinc, germanium, boron and dregs of beans, urea.Drop into 0.1～1.0% microbial composite bacteria kind, guarantee the aeration condition of fermentor tank, isolated insect.Guarantee maturation jar air-proof condition 2～10 months.
3) packing: supernatant liquor mixes in the feed and uses the biological growth agent as animal, and precipitation and suspension are used the biological growth agent as plant.
2. according to the described method for preparing the agent of sweet Stevia biological growth of claim 1, the sweet Stevia biological growth agent of preparation is used for the base manure, biological foliage fertilizer of crop etc., as the daily fodder additives of animal.
3. the preparation method of sweet Stevia biological growth according to claim 1 agent, it is characterized in that utilizing the maturation of fermenting of microbial composite bacteria kind, the bacterial classification of employing comprises beneficial microorganisms such as the microorganism of vinelandii, phosphate-solubilizing bacteria, potassium bacterium, photosynthetic bacterium, actinomycetes, product agricultural antibiotic and yeast, genus bacillus, bifidus bacillus, milk-acid bacteria, clostridium butyricum.
4. the preparation method of sweet Stevia biological growth according to claim 1 agent is characterized in that adding the mixing addition material.The inorganic salt that the addition material that adds comprises nitrogen, phosphorus, potassium and contains trace elements such as selenium, germanium, zinc, boron, molybdenum, copper, manganese.
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| CN201010108114XA CN102146000A (en) | 2010-02-10 | 2010-02-10 | Method for preparing stevia rebaudiana biological growth promoting agent |
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| CN201010108114XA CN102146000A (en) | 2010-02-10 | 2010-02-10 | Method for preparing stevia rebaudiana biological growth promoting agent |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515926A (en) * | 2012-01-05 | 2012-06-27 | 中国农业大学 | Composition for promoting growth of stevia rebaudiana, and preparation method for composition |
| CN103156057A (en) * | 2011-12-16 | 2013-06-19 | 邝素明 | Microbial fermented feed and production process thereof |
| CN104446942A (en) * | 2014-11-21 | 2015-03-25 | 苏州市吴中区光福香雪苗圃 | Novel bacterial fertilizer |
| CN105060973A (en) * | 2015-07-25 | 2015-11-18 | 安徽博发文化生态园有限公司 | Special foliage fertilizer for hickories |
| CN105669262A (en) * | 2016-01-26 | 2016-06-15 | 曲阜师范大学 | Microbial organic fertilizer produced by fermenting waste residues of stevia rebaudiana and cotton seeds as well as preparation method of fertilizer |
| CN106220418A (en) * | 2016-07-20 | 2016-12-14 | 杜云芳 | A kind of organic nutrition of plant agent and preparation method thereof |
| CN106387341A (en) * | 2016-08-31 | 2017-02-15 | 汪金小 | Broussonetia papyrifera fermented feed and preparation method thereof |
| CN107312548A (en) * | 2017-07-17 | 2017-11-03 | 河海大学 | A kind of organic improvement base for promoting solonchak sodium ion exchange |
| CN110981562A (en) * | 2019-09-16 | 2020-04-10 | 上海维丘康敬生物科技有限公司 | Use of stevia preparations in agriculture |
| CN112772770A (en) * | 2020-12-29 | 2021-05-11 | 广东冠龙生物科技有限公司 | Preparation method of stevia rebaudiana fermentation product and milch goat feed |
| WO2021132641A1 (en) * | 2019-12-28 | 2021-07-01 | Ｏａｔアグリオ株式会社 | Plant stomata opening promotion agent |
| WO2021132642A1 (en) * | 2019-12-28 | 2021-07-01 | Ｏａｔアグリオ株式会社 | Plant photosynthesis promotion agent |
| CN114886017A (en) * | 2022-04-20 | 2022-08-12 | 河南盛博原农牧有限公司 | Fermented feed additive for enhancing immunity of livestock and poultry and preparation method thereof |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156057A (en) * | 2011-12-16 | 2013-06-19 | 邝素明 | Microbial fermented feed and production process thereof |
| CN102515926A (en) * | 2012-01-05 | 2012-06-27 | 中国农业大学 | Composition for promoting growth of stevia rebaudiana, and preparation method for composition |
| CN104446942A (en) * | 2014-11-21 | 2015-03-25 | 苏州市吴中区光福香雪苗圃 | Novel bacterial fertilizer |
| CN105060973A (en) * | 2015-07-25 | 2015-11-18 | 安徽博发文化生态园有限公司 | Special foliage fertilizer for hickories |
| CN105669262B (en) * | 2016-01-26 | 2019-12-03 | 曲阜师范大学 | A kind of microbial organic fertilizer and preparation method thereof of STEVIA REBAUDIANA waste residue composite cotton fabric benevolence fermenting and producing |
| CN105669262A (en) * | 2016-01-26 | 2016-06-15 | 曲阜师范大学 | Microbial organic fertilizer produced by fermenting waste residues of stevia rebaudiana and cotton seeds as well as preparation method of fertilizer |
| CN106220418A (en) * | 2016-07-20 | 2016-12-14 | 杜云芳 | A kind of organic nutrition of plant agent and preparation method thereof |
| CN106387341A (en) * | 2016-08-31 | 2017-02-15 | 汪金小 | Broussonetia papyrifera fermented feed and preparation method thereof |
| CN107312548A (en) * | 2017-07-17 | 2017-11-03 | 河海大学 | A kind of organic improvement base for promoting solonchak sodium ion exchange |
| CN107312548B (en) * | 2017-07-17 | 2020-06-09 | 河海大学 | Organic state improved base for promoting sodium ion exchange of saline soil |
| CN110981562A (en) * | 2019-09-16 | 2020-04-10 | 上海维丘康敬生物科技有限公司 | Use of stevia preparations in agriculture |
| KR102604169B1 (en) | 2019-12-28 | 2023-11-17 | 오에이티 아그리오 가부시키가이샤 | plant stomata opening promoter |
| WO2021132641A1 (en) * | 2019-12-28 | 2021-07-01 | Ｏａｔアグリオ株式会社 | Plant stomata opening promotion agent |
| WO2021132642A1 (en) * | 2019-12-28 | 2021-07-01 | Ｏａｔアグリオ株式会社 | Plant photosynthesis promotion agent |
| KR102634846B1 (en) | 2019-12-28 | 2024-02-06 | 오에이티 아그리오 가부시키가이샤 | Plant photosynthesis promoter |
| KR20220122665A (en) * | 2019-12-28 | 2022-09-02 | 오에이티 아그리오 가부시키가이샤 | plant photosynthesis promoter |
| KR20220122664A (en) * | 2019-12-28 | 2022-09-02 | 오에이티 아그리오 가부시키가이샤 | plant pore opening promoter |
| CN112772770A (en) * | 2020-12-29 | 2021-05-11 | 广东冠龙生物科技有限公司 | Preparation method of stevia rebaudiana fermentation product and milch goat feed |
| CN114886017A (en) * | 2022-04-20 | 2022-08-12 | 河南盛博原农牧有限公司 | Fermented feed additive for enhancing immunity of livestock and poultry and preparation method thereof |
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Application publication date: 20110810 |