KR101260035B1 - nutrient agent for plant and manufacturing method the same - Google Patents
nutrient agent for plant and manufacturing method the same Download PDFInfo
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- KR101260035B1 KR101260035B1 KR1020110012243A KR20110012243A KR101260035B1 KR 101260035 B1 KR101260035 B1 KR 101260035B1 KR 1020110012243 A KR1020110012243 A KR 1020110012243A KR 20110012243 A KR20110012243 A KR 20110012243A KR 101260035 B1 KR101260035 B1 KR 101260035B1
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- 235000013379 molasses Nutrition 0.000 claims abstract description 49
- 241000196324 Embryophyta Species 0.000 claims abstract description 33
- 239000003337 fertilizer Substances 0.000 claims abstract description 29
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 238000010790 dilution Methods 0.000 claims abstract description 21
- 239000012895 dilution Substances 0.000 claims abstract description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 20
- 240000000111 Saccharum officinarum Species 0.000 claims abstract description 19
- 235000007201 Saccharum officinarum Nutrition 0.000 claims abstract description 19
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 14
- 235000000346 sugar Nutrition 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 8
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000011574 phosphorus Substances 0.000 claims abstract description 7
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000012141 concentrate Substances 0.000 claims abstract description 6
- 239000011591 potassium Substances 0.000 claims abstract description 6
- 239000002244 precipitate Substances 0.000 claims abstract description 6
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims abstract description 5
- 241000235070 Saccharomyces Species 0.000 claims abstract description 4
- 239000003085 diluting agent Substances 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 2
- 239000002994 raw material Substances 0.000 abstract description 15
- 235000013311 vegetables Nutrition 0.000 abstract description 9
- 230000008635 plant growth Effects 0.000 abstract description 7
- 150000001413 amino acids Chemical class 0.000 abstract description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 16
- 239000000126 substance Substances 0.000 description 10
- 229910052500 inorganic mineral Inorganic materials 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000011707 mineral Substances 0.000 description 7
- 235000010755 mineral Nutrition 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- 235000020971 citrus fruits Nutrition 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000005985 organic acids Nutrition 0.000 description 3
- 230000001863 plant nutrition Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 244000175448 Citrus madurensis Species 0.000 description 1
- 235000017317 Fortunella Nutrition 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 235000003953 Solanum lycopersicum var cerasiforme Nutrition 0.000 description 1
- 240000003040 Solanum lycopersicum var. cerasiforme Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
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Abstract
본 발명은 식물성 원료인 사탕수수 당밀(cane molasses)의 효모발효를 통해 기존의 동물성 원료 대비 상대적으로 풍부한 아미노산 함량을 얻을 수 있어 식물의 고품질 성장과 작물의 수확량 증가를 가능케 하는 친환경의 식물 영양제 및 이의 제조방법에 관한 것으로서,(a) Saccharomyces속, Candida속 중 어느 하나에 속하는 효모의 배양액을 얻는 단계; (b) 사탕수수 당밀에 물을 가해 총 당량 11~21 중량%의 당밀 희석액을 얻은 후 상기 당밀 희석액에 상기 배양액을 상기 당밀 희석액 중량 기준 20~30 중량% 혼합하여 25~35℃에서 60~80 시간 발효시켜 발효액을 얻는 단계; (c) 상기 발효액 하단의 침전물을 제거하고 전체중량기준 10~30 중량%의 고형분을 포함한 액체성분을 취한 후 실온에서 10~30% 농축된 농축액을 얻는 단계; (d) 상기 농축액에 질소(N), 인(P), 칼륨(K) 중 하나 이상을 함유한 단일 또는 복합비료를 혼합하여 N:P:K의 농도비율을 4(±1)%:4(±1)%:4(±1)%, 6(±1)%:3(±1)%:3(±1)%, 2(±1)%:9(±1)%:3(±1)%, 2(±1)%:6(±1)%:6(±1)%, 0(+1)%:0(+1)%:4(±1)% 중 하나로 결정하는 단계를 포함하는 식물 영양제의 제조방법 및 이를 통해 제조된 식물 영양제를 제공한다..The present invention can obtain a relatively rich amino acid content compared to the existing animal raw materials through the yeast fermentation of sugar cane molasses (cane molasses) as a vegetable raw material and environmentally friendly plant nutrients that enable high quality growth of plants and increased yield of crops and its In the manufacturing method, (a) obtaining a culture of yeast belonging to any one of the genus Saccharomyces , Candida ; (b) adding sugar to the sugar cane molasses to obtain a molasses dilution of 11 to 21 wt% of the total equivalent weight, and then mixed the culture solution with 20 to 30 wt% of the molasses diluent based on the weight of the molasses dilution at 60 to 80 ° C. Fermentation time to obtain a fermentation broth; (c) removing the precipitate at the bottom of the fermentation broth and taking a liquid component containing 10-30 wt% solids based on the total weight, to obtain a concentrate concentrated at 10-30% at room temperature; (d) The concentration ratio of N: P: K is 4 (± 1)%: 4 by mixing single or complex fertilizer containing one or more of nitrogen (N), phosphorus (P) and potassium (K) to the concentrate. (± 1)%: 4 (± 1)%, 6 (± 1)%: 3 (± 1)%: 3 (± 1)%, 2 (± 1)%: 9 (± 1)%: 3 ( Determined as ± 1)%, 2 (± 1)%: 6 (± 1)%: 6 (± 1)%, 0 (+1)%: 0 (+1)%: 4 (± 1)% It provides a method for producing a plant nutrient comprising the step and the plant nutrient produced by the same.
Description
본 발명은 식물 영양제(nutrient agent for plant) 및 이의 제조방법에 관한 것이다. 보다 구체적으로 본 발명은 식물성 원료인 사탕수수 당밀(cane molasses)의 효모발효를 통해 기존의 동물성 원료 대비 상대적으로 풍부한 아미노산 함량을 얻을 수 있어 식물의 고품질 성장과 작물의 수확량 증가를 가능케 하는 친환경의 식물 영양제 및 이의 제조방법에 관한 것이다.
The present invention relates to a nutrient agent for plant and a method for preparing the same. More specifically, the present invention can obtain a relatively rich amino acid content compared to the existing animal raw materials through the yeast fermentation of sugar cane molasses (cane molasses) as a vegetable raw material is an environmentally friendly plant that enables high-quality growth of plants and increased yield of crops It relates to a nutrient and a method for producing the same.
일반적으로 '비료(fertilizer)'란 식물의 생산성 향상을 위해 토양에 사용하는 천연 또는 인공물질을 총칭하며, 법률 5019호의 비료관리법은 '식물에 영양을 주거나 식물의 재배를 돕기 위하여 흙에서 화학적 변화를 가져오게 하는 물질과 식물에 영양을 주는 물질'로 정의하고 있다.In general, 'fertilizer' refers to the natural or artificial substances used in the soil to improve the productivity of the plant, and the fertilizer management law of Law 5019 states that 'chemical change in soil is used to nourish the plant or to help the plant's cultivation. 'Materials bringing substances and substances that give nutrition to plants'.
위 정의에 따르면 비료는 동식물성 원료로부터 얻어지는 '유기질비료' 내지는 '보통비료'와 무기질 원료의 화학적 처리를 통해 얻어지는 '무기질비료' 내지는 '화학비료'로 나누어볼 수 있는데, 통상적으로 '비료'라 지칭되는 무기질 비료는 그 구성성분에 따라 질소(N), 인(P), 칼륨(K)의 3요소 중 단일성분을 함유한 단일비료와 3요소 중 2가지 이상을 혼합한 복합비료로 구분 가능하고, 복합비료는 또다시 화학적 처리를 거친 1종 복합비료, 화학적 처리를 배제한 2종 복합비료, 유기질 주축의 3종 복합비료, 액제(液劑)·수화제(水和劑)·수용제(水溶劑) 등의 4종 복합비료로 세분화될 수 있다.According to the above definition, fertilizers can be divided into 'organic fertilizers' or 'regular fertilizers' obtained from animal and vegetable raw materials and 'inorganic fertilizers' or 'chemical fertilizers' obtained through chemical treatment of inorganic raw materials. The mineral fertilizers referred to can be classified into a single fertilizer containing a single component of three elements of nitrogen (N), phosphorus (P) and potassium (K) and a complex fertilizer in which two or more of the three elements are mixed according to its components. The compound fertilizer is once again a compound fertilizer that has undergone chemical treatment, two compound fertilizers without chemical treatment, three compound fertilizers with organic spindles, liquids, hydrating agents, and aqueous solutions. I) It can be subdivided into four complex fertilizers.
한편, 좋은 비료는 식물생육에 필요한 양분을 일정량 포함하고 식물생육과 환경보전에 유해물질이 없으며 수송·저장·사용이 편리하고 가격이 저렴하며 비효(肥效)가 높을 필요가 있다. 무기질 비료는 화학공업에 의해 대량 생산되므로 품질이 균일하고 공급이 안정적이며 가격이 저렴하다는 점, 유기질 비료에 비해 위생적이고 사용이 편리하다는 점, 질소(N), 인(P) 등의 유효성분의 함량이 높고 일정하다는 점, 수송비용이 저렴하고 저장이 용이하다는 점, 목적에 적합한 시비(fertilization)가 가능하다는 점 등을 이유로 대부분의 농가에서 널리 사용되고 있다.On the other hand, good fertilizers contain a certain amount of nutrients necessary for plant growth, no harmful substances for plant growth and environmental conservation, convenient transportation, storage and use, low cost, and high efficiency. Inorganic fertilizers are produced in large quantities by the chemical industry, so they are uniform in quality, stable in supply, and inexpensive. They are more hygienic and easier to use than organic fertilizers. Nitrogen (N) and phosphorus (P) It is widely used in most farms because of its high content and constant content, low transportation cost and easy storage, and fertilization suitable for the purpose.
하지만, 무기질 비료의 과잉사용은 토양을 산성화시키고 유기물의 함량감소를 초래하여 지력(soil productivity)을 떨어뜨리는 등 장기적으로는 토양의 단립(aggregation)을 파괴하여 식물에 해를 입힐 수 있다. 이에 따라 실제로 국내 주요농경지에 대한 인산(phosphoric acid) 집적비율은 적정수준을 초과하고 있고, 비료사용이 많은 지역을 중심으로 토양의 유기물부족과 통기성 및 보수성 악화로 인한 식물의 생육부진 등 각종 피해사례가 나타나고 있다.However, over-use of mineral fertilizers can damage plants in the long term, destroying soil aggregation, such as acidifying the soil and reducing organic matter content. As a result, the accumulation ratio of phosphoric acid in major agricultural lands in Korea exceeds the appropriate level, and various damage cases such as poor growth of plants due to lack of organic matter in soil and deterioration of breathability and water conservancy in areas where fertilizer use is high Is appearing.
그 결과 현재로서는 식물의 생육촉진과 작물의 수확량 증가 등 비료 고유의 역할과 더불어 장기간 사용에 따른 피해감소 등 농업환경보전에 근본적으로 이바지할 수 있는 친환경적인 방도가 시급히 요구되고 있는 실정이다.
As a result, there is an urgent need for environmentally friendly measures that can contribute fundamentally to the preservation of the agricultural environment, such as reducing the damage caused by long-term use, as well as the inherent role of fertilizers such as promoting plant growth and increasing crop yields.
본 발명은 상기와 같은 실정을 감안하여 안출된 것으로서 식물성 원료로부터 얻어진 친환경 식물 영양제를 제공하는데 목적을 둔다. The present invention has been made in view of the above circumstances, and an object thereof is to provide an eco-friendly phytonutrient obtained from vegetable raw materials.
구체적으로 본 발명은 식물성 원료를 사용하면서도 기존의 동물성 원료 대비 상대적으로 풍부한 아미노산, 유기산, 당분, 미네랄 함량을 얻을 수 있어 식물의 고품질 성장과 작물의 수확량 증가는 물론 장기간 사용에 따른 피해감소를 통해 농업환경보전에 일조할 수 있는 친환경의 식물 영양제 및 이의 제조방법을 제공하는데 그 목적을 둔다.
Specifically, the present invention can obtain abundant amino acids, organic acids, sugars and minerals content compared to the existing animal raw materials while using vegetable raw materials, so that high-quality growth of plants and increased yields of crops, as well as reduced damage caused by long-term use The purpose of the present invention is to provide environmentally friendly plant nutrition and its manufacturing method that can contribute to environmental conservation.
본 발명은 상기와 같은 목적을 달성하기 위하여 (a) Saccharomyces속, Candida속 중 어느 하나에 속하는 효모의 배양액을 얻는 단계; (b) 사탕수수 당밀에 물을 가해 총 당량 11~21 중량%의 당밀 희석액을 얻은 후 상기 당밀 희석액에 상기 배양액을 상기 당밀 희석액 중량 기준 20~30 중량% 혼합하여 25~35℃에서 60~80 시간 발효시켜 발효액을 얻는 단계; (c) 상기 발효액 하단의 침전물을 제거하고 전체중량기준 10~30 중량%의 고형분을 포함한 액체성분을 취한 후 실온에서 10~30% 농축된 농축액을 얻는 단계; (d) 상기 농축액에 질소(N), 인(P), 칼륨(K) 중 하나 이상을 함유한 단일 또는 복합비료를 혼합하여 N:P:K의 농도비율을 4(±1)%:4(±1)%:4(±1)%, 6(±1)%:3(±1)%:3(±1)%, 2(±1)%:9(±1)%:3(±1)%, 2(±1)%:6(±1)%:6(±1)%, 0(+1)%:0(+1)%:4(±1)% 중 하나로 결정하는 단계를 포함하는 식물 영양제의 제조방법을 제공한다.The present invention to achieve the above object (a) obtaining a culture solution of yeast belonging to any one of the genus Saccharomyces , Candida ; (b) adding sugar to the sugar cane molasses to obtain a molasses dilution of 11 to 21 wt% of the total equivalent weight, and then mixed the culture solution with 20 to 30 wt% of the molasses diluent based on the weight of the molasses dilution at 60 to 80 ° C. Fermentation time to obtain a fermentation broth; (c) removing the precipitate at the bottom of the fermentation broth and taking a liquid component containing 10-30 wt% solids based on the total weight, to obtain a concentrate concentrated at 10-30% at room temperature; (d) The concentration ratio of N: P: K is 4 (± 1)%: 4 by mixing single or complex fertilizer containing one or more of nitrogen (N), phosphorus (P) and potassium (K) to the concentrate. (± 1)%: 4 (± 1)%, 6 (± 1)%: 3 (± 1)%: 3 (± 1)%, 2 (± 1)%: 9 (± 1)%: 3 ( Determined as ± 1)%, 2 (± 1)%: 6 (± 1)%: 6 (± 1)%, 0 (+1)%: 0 (+1)%: 4 (± 1)% It provides a method for producing a plant nutrient comprising the step.
이때, 상기 (a) 단계는 상기 효모를 배지에 접종하고 25~35℃에서 40~60rpm으로 20~30 시간 동안 1차 배양하는 단계; 사탕수수 당밀에 물을 더해 총 당량 5~15 중량%의 당밀 희석액을 얻은 후 상기 당밀 희석액에 상기 배양액을 상기 당밀 희석액 중량기준 5~15 중량% 접종하여 25~35℃에서 40~60 rpm으로 20~30 시간 2차 배양하는 단계를 포함하는 것을 특징으로 한다.At this time, the step (a) is inoculated in the yeast medium and the step of primary culture for 20-30 hours at 40 ~ 60rpm at 25 ~ 35 ℃; Water was added to sugarcane molasses to obtain a molasses dilution of 5-15 wt%, and the culture solution was inoculated with 5 to 15 wt% of the molasses dilution based on the weight of the molasses dilution 20 at 40 to 60 rpm. It is characterized in that it comprises a step of secondary culture for ˜30 hours.
또한 상기 사탕수수 당밀은 하기의 성분분석을 갖는 밀도 75~85 브릭스(brix)인 것을 특징으로 한다.In addition, the sugar cane molasses is characterized in that the density 75 ~ 85 Brix (brix) having the following component analysis.
그리고 본 발명은 상기의 방법으로 제조되어 N:P:K의 농도비율이 4(±1)%:4(±1)%:4(±1)%, 6(±1)%:3(±1)%:3(±1)%, 2(±1)%:9(±1)%:3(±1)%, 2(±1)%:6(±1)%:6(±1)%, 0(+1)%:0(+1)%:4(±1)% 중 하나이고, 하기의 유기산, 당분, 아미노산, 미네랄 성분을 공통으로 포함하는 식물 영양제를 제공한다.And the present invention is prepared by the above method N: P: K concentration ratio of 4 (± 1)%: 4 (± 1)%: 4 (± 1)%, 6 (± 1)%: 3 (± 1)%: 3 (± 1)%, 2 (± 1)%: 9 (± 1)%: 3 (± 1)%, 2 (± 1)%: 6 (± 1)%: 6 (± 1 Provided is a phytonutrient comprising one of%), 0 (+1)%: 0 (+1)%: 4 (± 1)%, and commonly containing the following organic acids, sugars, amino acids and minerals.
본 발명에 따른 식물 영양제는 사탕수수 당밀의 효모발효를 통해 식물성 원료를 사용하면서도 기존의 동물성 원료 대비 상대적으로 풍부한 아미노산, 유기산, 당분, 미네랄 함량을 얻을 수 있는 장점이 있다.The plant nutritional agent according to the present invention has an advantage of using a vegetable raw material through yeast fermentation of sugarcane molasses to obtain a relatively rich amino acid, organic acid, sugar, mineral content compared to the existing animal raw material.
특히 본 발명에 따른 식물 영양제는 질소(N), 인(P), 칼륨(K) 중 하나 이상을 함유한 단일 또는 복합비료와 적절히 혼합 사용할 경우 과수류, 과실류, 엽채류, 화목류, 화초류, 감귤류, 과채류, 관엽식물, 잔디류 등에 대해 생육촉진, 수확량 증가, 당도증가, 감산효과, 토양개량의 효과와 더불어 입의 색이 좋아지고 굵기가 커지는 효과를 얻을 수 있었다.In particular, the plant nutrients according to the present invention, when properly mixed with a single or complex fertilizer containing one or more of nitrogen (N), phosphorus (P), potassium (K), fruit trees, fruits, leafy vegetables, tree plants, plants, citrus fruits, fruits and vegetables In addition to the effects of growing growth, yield, sugar content, reduction effect, and soil improvement on plant, grass, etc., the color of mouth was improved and the thickness was increased.
더 나아가 본 발명에 따른 식물 영양제는 식물성 원료를 사용함에 따라 장기간 사용에 따른 피해감소와 농업환경보전에 일조할 수 있는 장점이 있다.
Furthermore, the plant nutrients according to the present invention have the advantage of helping to reduce the damage caused by long-term use and to preserve the agricultural environment by using vegetable raw materials.
도 1은 본 발명에 따른 식물 영양제의 제조공정을 나타낸 모식도.
도 2는 본 발명에 따른 식물 영양제의 제조공정을 나타낸 순서도.1 is a schematic diagram showing a manufacturing process of the plant nutrient according to the present invention.
Figure 2 is a flow chart showing the manufacturing process of the plant nutrient according to the present invention.
이하, 도면을 참조하여 본 발명의 일 양태(樣態)를 상세히 살펴본다. EMBODIMENT OF THE INVENTION Hereinafter, one aspect of this invention is looked at in detail with reference to drawings.
도 1과 도 2는 각각 본 발명에 따른 식물 영양제의 제조공정을 나타낸 모식도와 순서도이다.1 and 2 are a schematic diagram and a flow chart showing the manufacturing process of the plant nutrition according to the present invention, respectively.
보이는 것처럼, 본 발명에 따른 식물 영양제는 사탕수수 당밀의 효모발효를 통해 얻어지며, 이를 위한 원료준비단계(st1), 배양단계(st2), 발효단계(st3), 농축단계(st4)를 포함한다.As shown, the phytonutrient according to the present invention is obtained through the yeast fermentation of sugar cane molasses, and includes a raw material preparation step (st1), culture step (st2), fermentation step (st3), concentration step (st4) for this. .
각각을 상세히 살펴보면 다음과 같다.Looking at each of them in detail as follows.
원료준비단계(st1)에서는 주원료인 사탕수수 당밀(2)과 효모(4)를 준비한다.(st1) In the raw material preparation step (st1), sugar cane molasses (2) and yeast (4), which are main raw materials, are prepared. (St1)
본 발명에서 사용하는 사탕수수 당밀(2)은 설탕의 제조 또는 정제 후 부산물로서 바람직하게는 박화당으로 표시되는 총 당분을 45% 이상 함유한다. 이때, 일반적인 사탕수수 당밀(2)은 조성이 일정치 않고 색, 향기, 점도, 당분함량, 비중 등에 상당한 차이가 있으므로 본 발명에서는 밀도 75~85 브릭스(brix)로 물을 혼합한 이른바 블랙스트립 당밀(blackstrap molasses)를 사용한다.The
표 1은 본 발명에서 사용하는 사탕수수 당밀(2)의 주성분을 나타낸다.Table 1 shows the main components of the
참고로 본 발명에서 사용하는 사탕수수 당밀(2) 내 대량무기물은 Na 0.1~0.4 중량%, K 1.5~5.0 중량%, Ca 0.4~0.8 중량%, Cl 0.7~3.0 중량%, P 0.03~0.1 중량%, S 0.3~0.8 중량%, Mg 0.05~0.98 중량% 등이고, 미량무기물은 Cu 2.2~3.8 mg/kg, Fe 30~500 mg/kg, Zn 4.0~4.8 mg/kg, Mn 4~300 mg/kg, Co 0.3~2.0 mg/kg, Pb 0.5~5.0 mg/kg, Cd 0.4 mg/kg 이하, As 0.15 mg/kg 이하, Al 0.01~0.02 mg/kg를 포함한다.For reference, the bulk minerals in the sugarcane molasses (2) used in the present invention are Na 0.1 ~ 0.4% by weight, K 1.5 ~ 5.0% by weight, Ca 0.4 ~ 0.8% by weight, Cl 0.7 ~ 3.0% by weight, P 0.03 ~ 0.1 weight %, S 0.3 ~ 0.8% by weight, Mg 0.05 ~ 0.98% by weight, trace minerals are Cu 2.2 ~ 3.8 mg / kg, Fe 30 ~ 500 mg / kg, Zn 4.0 ~ 4.8 mg / kg,
또한, 본 발명에서 사용하는 효모(4)는 자낭균류의 Saccharomyces속, Candida속 등이며 바람직하게는 Saccharomyces cerevisiae , Candida utilis 중 하나를 사용한다.In addition, the yeast (4) used in the present invention is Saccharomyces genus, Candida genus and the like of the aseptic fungus, preferably Saccharomyces cerevisiae , Candida Use one of utilis
다음으로, 배양단계(st2)에서는 효모(4)를 배양하여 효모 배양액을 얻는다.Next, in the culture step (st2), the
본 발명에서는 바람직하게는 YMEA 배지에 효모(4)를 접종하여 25~35℃의 온도, 40~60rpm으로 20~30 시간 활성화한 후 사탕수수 당밀(2)에 물을 혼합하여 총 당량 5~15 중량%로 희석한 제 1 당밀 희석액에 그 중량기준 5~15 중량 % 접종해서 25~35℃에서 40~60rpm으로 20~30시간 배양한다. 이때, 배양단계의 전 과정은 무균환경에서 진행되고, 일례로 무균살균되고 밀폐가능한 철재의 제 1 탱크(12)에서 제 1 당밀 희석액에 배양액을 접종 및 배양한다.In the present invention, preferably inoculated with yeast (4) in YMEA medium and activated for 20 to 30 hours at a temperature of 25 ~ 35 ℃, 40 ~ 60rpm and then mixed with sugar cane molasses (2) water equivalent 5-15 Inoculate 5-15% by weight in the first molasses dilution diluted to weight% and incubated at 25-35 ° C. at 40-60 rpm for 20-30 hours. At this time, the whole process of the culturing step is carried out in a sterile environment, for example, inoculated and cultured in the first molasses diluent in the
다음으로, 발효단계(st3)에서는 사탕수수 당밀(2)에 효모 배양액을 혼합 및 발효하여 당밀 발효액을 얻는다.Next, in the fermentation stage (st3), the yeast culture solution is mixed and fermented to the
본 발명에서는 바람직하게는 사탕수수 당밀(2)에 물을 혼합하여 총 당량 11~21 중량%로 희석한 제 2 당밀 희석액에 그 중량기준 20~30 중량%의 배양액을 혼합하여 25~35℃에서 60~80 시간 발효한다. 이때, 발효단계의 전 과정 역시 무균환경에서 진행되고, 일례로 무균살균되고 밀폐가능한 철재의 제 2 탱크(22)에서 진행될 수 있다. 그리고 발효액을 수시간 동안 침전시켜 발효액 하단의 침전물(42)인 잔류 효모류 등을 제거하여 전체 중량기준 10~30 중량%의 고형분을 포함한 액체성분(32), 즉 당밀 발효액을 얻는다. In the present invention, preferably the sugar mixture molasses (2) is mixed with water in a second molasses dilution diluted to 11 to 21% by weight of the total equivalent weight of the culture solution of 20 to 30% by weight of the mixture at 25 ~ 35 ℃ Ferment 60-80 hours. At this time, the whole process of the fermentation step may also proceed in a sterile environment, for example, may be performed in a
참고로, 본 단계에서 제거된 침전물(42)은 별도의 과정을 통해 건조되어 고형사료 또는 고형비료(46) 등에 사용될 수 있다.For reference, the
다음으로, 농축단계(st4)에서는 당밀 발효액을 실온에서 10~30% 농축한다. Next, in the concentration step (st4) the molasses fermentation broth is concentrated 10 ~ 30% at room temperature.
그 결과 본 발명에 따른 식물 영양제(36)가 완성된다.As a result, the
이때, 본 발명에 따른 식물 영양제는 13 여종의 아미노산, 7 여종의 유기산, 10 여종의 미네랄류, 전당분을 포함하는데, 아래의 표 2는 본 발명에 따른 식물 영양제의 공통성분결과이다.At this time, the plant nutritional agent according to the present invention includes 13 kinds of amino acids, 7 kinds of organic acids, 10 kinds of minerals, and starch, Table 2 below is a common ingredient result of the plant nutritional agent according to the present invention.
이하, 본 발명의 실시예를 살펴본다.
Hereinafter, an embodiment of the present invention will be described.
자낭균류인 Saccharomyces cerevisiae(KCCM11304)를 YMEA 배지(yeast extract 0.3 중량%, malt extract 0.3 중량%, peptone 0.5 중량%, glucose 1 중량%)에 접종하고 30℃에서 50rpm으로 1.5일 배양하여 효모를 활성화시켰다. 그리고 하기의 표 3의 성분분석을 갖는 사탕수수 당밀에 증류수를 더해 총 당량 10 중량%의 제 1 당밀 희석액을 얻은 후 밀폐 가능한 원통 형상의 제 1 탱크에 제 1 당밀 희석액을 담고 그 중량기준 10 중량%의 배양액을 접종하여 30℃에서 50rpm으로 1.5일 배양하여 배양액을 얻었다.Saccharomyces cerevisiae (KCCM11304), which is the aseptic fungus, was inoculated in YMEA medium (0.3% by weight of yeast extract, 0.3% by weight of malt extract, 0.5% by weight of peptone, 1% by weight of glucose) and incubated for 1.5 days at 50 ° C at 30 ° C to activate yeast. . And distilled water was added to sugarcane molasses having the component analysis of Table 3 below to obtain a first molasses dilution of 10% by weight of the total equivalent, the first molasses dilution is contained in a sealed cylindrical first tank containing 10 wt% % Of the culture was inoculated and incubated for 1.5 days at 30 rpm at 50 rpm to obtain a culture.
이어서 동일성분의 사탕수수 당밀에 증류수를 더해 총 당량 16 중량%의 제 2 당밀 희석액을 얻은 후 밀폐 가능한 원통 형상의 제 2 탱크에 제 2 당밀 희석액을 담고 그 중량기준 25 중량%의 배양액을 혼합하여 30℃에서 3일간 발효하여 발효액을 얻었으며, 마지막으로 5시간 침전 후 발효액 하단의 침전물인 잔류 효모류를 제거하고 전체중량기준 20 중량%의 고형분을 포함한 액체성분을 취해 실온에서 20% 농축하여 본 발명의 일 실시예에 따른 식물 영양제를 완성하였다.Subsequently, distilled water was added to sugarcane molasses of the same component to obtain a second molasses dilution of 16% by weight in total, and then the second molasses dilution was contained in a sealed cylindrical tank, and 25% by weight of the culture solution was mixed. The fermentation broth was obtained by fermentation at 30 ° C. for 3 days, and finally, after 5 hours of precipitation, residual yeasts, which are precipitates at the bottom of the fermentation broth, were removed, and a liquid component containing 20% by weight of solids based on the total weight was taken and concentrated at room temperature for 20%. To complete the plant nutrition according to one embodiment of the.
아래의 표 4는 위 과정을 거쳐 완성된 본 발명의 일 실시예에 따른 식물 영양제의 성분분석결과이다.Table 4 below is a component analysis result of the plant nutrient according to an embodiment of the present invention completed through the above process.
한편, 본 발명에 따른 식물 영양제는 바람직하게는 질소(N), 인(P), 칼륨(K) 중 하나 이상을 함유한 단일 또는 복합비료와 혼합 사용하며, 일례로 N:P:K의 농도비율은 N, P, K 각각의 오차범위 ±1% 범위 내에서 4(±1)%:4(±1)%:4(±1)%, 6(±1)%:3(±1)%:3(±1)%, 2(±1)%:9(±1)%:3(±1)%, 2(±1)%:6(±1)%:6(±1)%, 0%(+1):0(+1)%:4(±1)% 중 하나로 결정된다. (이하 괄호 내 오차범위의 표시는 생략한다.)On the other hand, plant nutrients according to the present invention is preferably used in combination with a single or complex fertilizer containing one or more of nitrogen (N), phosphorus (P), potassium (K), for example, the concentration of N: P: K Rate is 4 (± 1)%: 4 (± 1)%: 4 (± 1)%, 6 (± 1)%: 3 (± 1) within ± 1% of error range of N, P, K %: 3 (± 1)%, 2 (± 1)%: 9 (± 1)%: 3 (± 1)%, 2 (± 1)%: 6 (± 1)%: 6 (± 1)% , 0% (+ 1): 0 (+1)%: 4 (± 1)%. (Hereinafter, the indication of the error range in parentheses is omitted.)
그리고 이들 각각은 과수류, 과실류, 엽채류, 화목류, 화초류, 감귤류, 과채류, 관엽식물, 잔디류 등의 작물의 종류 내지는 성장시기 등에 따라 500~5000 배로 희석되어 1~2주 간격으로 토양관주 또는 엽면살포 되는데, 아래의 표 5 내지 표 9는 N:P:K 농도비율 4%:4%:4%(표 5), 6%:3%:3%(표 6), 2%:9%:3%(표 7), 2%:6%:6%(표 8), 0%:0%:4%(표 9)에 대한 적절한 적용작물, 사용시기, 희석배율, 효과, 시비방법을 나타낸다.Each of them is diluted 500-5000 times depending on the type of crops such as fruit trees, fruits, leafy vegetables, tree plants, flowers, citrus fruits, fruits, plants, grasses, etc. Tables 5 to 9 below show N: P:
또한, 표 10은 본 발명에 따른 식물 영양제의 실제사용효과를 나타낸 것으로서 2009년 10월부터 2010년 5월에 걸쳐 일본 나고야현에 위치한 비닐하우스에서 5개의 딸기(품종:사가호노카) 모종에 대해 본 발명에 따른 식물 영양제와 일반적인 액체비료(N:P:K 농도비율 16:16%:10%)를 각각 시비하여 월별 수확량과 당도를 비교한 결과이다. 여기에 사용된 본 발명에 따른 식물 영양제의 구체적인 종류 및 희석배율은 표 11에 나타내었다.In addition, Table 10 shows the actual use effect of the plant nutrients according to the present invention as seen for five strawberry (variety: Saga Honoka) seedlings in a plastic house located in Nagoya, Japan from October 2009 to May 2010 Plant yields and general liquid fertilizers (N: P: K concentration ratio 16: 16%: 10%) according to the invention was fertilized, respectively, and the results are compared with monthly yield. Specific types and dilution ratios of plant nutrients according to the present invention used herein are shown in Table 11.
마찬가지로, 표 12는 2009년 10월부터 2010년 5월에 걸쳐 일본 나고야현에 위치한 비닐하우스에서 5개의 방울토마토(품종:산체리퓨어) 모종에 대해 본 발명에 따른 식물 영양제와 일반적인 액체비료(N:P:K 농도비율 10%:4%:6%, 6%:8%:8%)를 각각 시비하여 월별 수확량과 당도를 비교한 결과이다. 여기에 사용된 본 발명에 따른 식물 영양제의 구체적인 종류 및 희석배율은 표 13에 나타내었다.Similarly, Table 12 shows plant nutrients and general liquid fertilizers (N) according to the present invention for five cherry tomato seedlings in a greenhouse in Nagoya, Japan, from October 2009 to May 2010. : P: K concentration ratio 10%: 4%: 6%, 6%: 8%: 8%) was fertilized respectively to compare the monthly yield and sugar content. Specific types and dilution ratios of plant nutrients according to the present invention used herein are shown in Table 13.
그외에도 텃밭감귤, 금귤(품종:네이하), 관목, 잔디 등에 본 발명에 따른 식물 영양제를 시비한 결과 일반적인 액체비료 대비 과실류의 경우 과실비대, 색감개선, 당도향상, 신맛감소 등의 효과를, 관목의 경우 생장촉진 등의 효과를, 잔디의 경우 밀도증가, 잎수증가, 뿌리생장촉진 등의 효과를 얻을 수 있었다.In addition, as a result of fertilizing the plant nutrients according to the present invention in garden citrus fruits, kumquat (variety: naha), shrubs, grass, etc., the effects of fruit hypertrophy, color improvement, sweetness improvement, sourness reduction, etc. In the case of shrubs, growth was enhanced, and in the case of grass, density was increased, leaf number was increased, and root growth was achieved.
Claims (4)
(b) 사탕수수 당밀에 물을 가해 총 당량 11~21 중량%의 당밀 희석액을 얻은 후 상기 당밀 희석액에 상기 배양액을 상기 당밀 희석액 중량 기준 20~30 중량% 혼합하여 25~35℃에서 60~80 시간 발효시켜 발효액을 얻는 단계;
(c) 상기 발효액 하단의 침전물을 제거하고 전체중량기준 10~30 중량%의 고형분을 포함한 액체성분을 취한 후 실온에서 최초 농도 대비 10~30% 비율로 농축된 농축액을 얻는 단계; 및
(d) 상기 농축액에 질소(N), 인(P), 칼륨(K) 중 하나 이상을 함유한 단일 또는 복합비료를 혼합하여 N:P:K의 농도비율을 4(±1)%:4(±1)%:4(±1)%, 6(±1)%:3(±1)%:3(±1)%, 2(±1)%:9(±1)%:3(±1)%, 2(±1)%:6(±1)%:6(±1)%, 0(+1)%:0(+1)%:4(±1)% 중 하나로 결정하는 단계를 포함하되,
상기 (a) 단계는,
상기 효모를 배지에 접종하고 25~35℃에서 40~60rpm으로 20~30 시간 동안 1차 배양하는 단계; 및
사탕수수 당밀에 물을 더해 총 당량 5~15 중량%의 당밀 희석액을 얻은 후 상기 당밀 희석액에 상기 배양액을 상기 당밀 희석액 중량기준 5~15 중량% 접종하여 25~35℃에서 40~60 rpm으로 20~30 시간 2차 배양하는 단계를 포함하는 식물 영양제의 제조방법.
(a) obtaining a culture of yeast belonging to any one of the genus Saccharomyces , Candida ;
(b) adding sugar to the sugar cane molasses to obtain a molasses dilution of 11 to 21 wt% of the total equivalent weight, and then mixed the culture solution with 20 to 30 wt% of the molasses diluent based on the weight of the molasses dilution at 60 to 80 ° C. Fermentation time to obtain a fermentation broth;
(c) removing the precipitate at the bottom of the fermentation broth and taking a liquid component containing 10-30 wt% of solids based on the total weight, to obtain a concentrate concentrated at 10-30% of the initial concentration at room temperature; And
(d) The concentration ratio of N: P: K is 4 (± 1)%: 4 by mixing single or complex fertilizer containing one or more of nitrogen (N), phosphorus (P) and potassium (K) to the concentrate. (± 1)%: 4 (± 1)%, 6 (± 1)%: 3 (± 1)%: 3 (± 1)%, 2 (± 1)%: 9 (± 1)%: 3 ( Determined as ± 1)%, 2 (± 1)%: 6 (± 1)%: 6 (± 1)%, 0 (+1)%: 0 (+1)%: 4 (± 1)% Including steps,
The step (a)
Inoculating the yeast in a medium and first culturing for 20-30 hours at 25-35 ° C. at 40-60 rpm; And
Water was added to sugarcane molasses to obtain a molasses dilution of 5-15 wt%, and the culture solution was inoculated with 5 to 15 wt% of the molasses dilution based on the weight of the molasses dilution 20 at 40 to 60 rpm. Method for producing a plant nutrient comprising a step of secondary culture ~ 30 hours.
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| CN103274800A (en) * | 2013-06-21 | 2013-09-04 | 西南大学 | Method for producing special flue-cured tobacco organic fertilizer by processing apple juicing waste residues |
| CN104230603A (en) * | 2014-09-19 | 2014-12-24 | 广西地源之本肥业有限公司 | Environment-friendly organic and inorganic fertilizer prepared with molasses alcohol waste mash used as raw material |
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