CN102125193A - Safe compound feed for crablets - Google Patents
Safe compound feed for crablets Download PDFInfo
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- CN102125193A CN102125193A CN2011100873589A CN201110087358A CN102125193A CN 102125193 A CN102125193 A CN 102125193A CN 2011100873589 A CN2011100873589 A CN 2011100873589A CN 201110087358 A CN201110087358 A CN 201110087358A CN 102125193 A CN102125193 A CN 102125193A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a safe compound feed for crablets, comprising soya bean meal, cotton seed meal, rapeseed meal, peanut meal, fish meal, fermenting protein peptides for aquaculture, squid extracts, small shrimp meal, phospholipid oil, fish oil, calcium biphosphate, flour, unshelling growth-promoting powder, trace elements for crablets, compound vitamins for crablets, 50% choline chloride, gene recombinant proteins and nutrient immune type aquatic phagostimulant. The invention can effectively inhibit various enteropathogenic bacteria by utilizing a gene recombinant protein growth immune promoter, the nutrient immune type aquatic phagostimulant and the fermenting protein peptides with high antibacterial peptide content, improve the digestibility of the nutrient components of the safe compound feed, promotes the growth of the crablets, also enhance the organism immunity of the crablets and remarkably increase the survival rate of the crablets.
Description
Technical field
The present invention relates to the feed of a kind of aquatic livestock.
Background technology
The feed of existing aquatic livestock, kind is numerous, and is nutritious mostly, but can not effectively improve immunity and the resistance against diseases of aquatic livestock.
Summary of the invention
The object of the present invention is to provide a kind of young crab safe compound feed that can significantly improve young crab survival rate.
Technical solution of the present invention is:
A kind of young crab safe compound feed is characterized in that: be made up of weight components:
Dregs of beans 15 ~ 20%
The cottonseed dregs of rice 6 ~ 7%
The dish dregs of rice 20 ~ 30%
Peanut meal 8 ~ 12%
Fish meal 3 ~ 5%
Aquatic products fermentation protein peptide 5 ~ 9%
Cuttlefish cream 2 ~ 3%
Shrimp head powder 2 ~ 3%
Phosphatide oil 1 ~ 3%
Fish oil 1 ~ 2%
Calcium dihydrogen phosphate 1 ~ 2%
Flour 15 ~ 20%
Shelling plain 0.05 ~ 0.2%
Crab is with micro-0.5 ~ 1.5%
Crab is used B B-complex 0.1 ~ 0.3%
50% Choline Chloride 0.1 ~ 0.3%
Gene recombinant protein 0.05 ~ 0.2%
Nutrition immune type aquatic attractant 0.4 ~ 0.6%;
Above-mentioned each amounts of components sum is 100%;
Crab is the mixture of B B-complex and carrier with B B-complex, and carrier is a time powder, and per 1000 gram crabs are with containing vitamin A 10000000IU, vitamin D in the B B-complex
32000000IU, vitamin E 75000IU, vitamin K
37500mg, Cobastab
120000mg, Cobastab
225000mg, Cobastab
620000mg, Cobastab
1220mg, nicotinic acid 60000mg, calcium pantothenate 25000mg, folic acid 2500mg, biotin 200mg, vitamin C 150000mg, inositol 100000mg;
Crab uses trace mineral supplement by the mixture of trace element with carrier, carrier is a zeolite, and per 1000 gram crabs are with containing iron 15000mg, manganese 3000mg, copper 2000mg, zinc 6000mg, cobalt 500mg, iodine 1000mg, selenium 100mg, magnesium 50000mg, potassium 25000mg in the trace mineral supplement.
Described aquatic products is made by following method with the fermentation protein peptide:
(1) configuration bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus fermenti are used for ferment tank and cultivate with four kinds of culture mediums; Wherein the bacillus subtilis culture medium prescription is: peptone 1wt%, and yeast extract 0.5 wt %, sodium chloride 0.5 wt %, dipotassium hydrogen phosphate 0.5 wt %, all the other are water; The Yeast Cultivation based formulas is: brewer's wort 20 wt %, and all the other are water; The culture medium prescription of streptococcus lactis and lactobacillus fermenti is: peptone 10 weight portions, powdered beef 5 weight portions, dusty yeast 4 weight portions, glucose 20 weight portions, Tween 80 1 weight portion dipotassium hydrogen phosphate 2 weight portions, sodium acetate 5 weight portions, Triammonium citrate 2 weight portion magnesium sulfate 0.2 weight portion, manganese sulfate 0.05 weight portion, agar powder 15 weight portions, distilled water 1000 weight portions; Above-mentioned each culture medium is all through 121 ℃ of autoclaving 15 ~ 20min;
(2) fermentation tank sterilization, inoculate after the cooling: respectively from jar mouth flame method, insert streptococcus lactis, lactobacillus fermenti in 1, No. 2 jar, carry out anaerobism and cultivate, each hour stirs once culture medium in the tank body; Respectively at inserting saccharomycete, bacillus subtilis in 3, No. 4 jars, carry out aerobic cultivation, rotating speed 175r/min, throughput are 0.8m
3/ min, tank pressure 0.03～0.05 Mpa;
(3) cultivating after fermentation in 12 ~ 24 hours finishes.Emitting bacterium liquid by measuring tank from four fermentation tanks mixes in blending tank, mixed bacteria liquid is stored in the receiver, feeds bacterium liquid from receiver in solid blender, and bacterium liquid is mixed with dregs of beans, mixed proportion is 1:2, and incorporation time is 5 minutes;
(4) the even accumulation of mixed material is distributed in ground between solid fermentation, highly is 0.6 meter, loam cake film, sealing all around.Carried out anaerobic fermentation 100 ~ 150 hours; Material after will fermenting again carries out 30 ~ 80 ℃ of low temperature dryings, pulverizing obtains aquatic products fermentation protein peptide.
Described gene recombinant protein is made by following method:
(1) in fermentation tank, disposes IGF respectively
Genetic engineering bacterium GD-001 and adenylate cyclase gene engineering bacteria GD-002 culture medium: IGF
Genetic engineering bacterium GD-001 and adenylate cyclase gene engineering bacteria GD-002 culture medium prescription are: peptone 1wt%, and yeast extract 0.5wt%, sodium chloride 0.5%, dipotassium hydrogen phosphate 0.5wt%, all the other are water; Culture medium is handled through 121 ℃ of autoclaving 20min;
(2) each adds the edible oil that is equivalent to culture medium weight 0.02 ~ 0.05% in fermentation, to eliminate barm;
(3) to fermentation tank culture medium sterilization and cooling, inoculate then: in No. 1 jar, insert IGF
The aerobic cultivation of genetic engineering bacterium GD-001 20 ~ 30 hours, rotating speed 175r/min, throughput are 1m
3/ min, tank pressure 0.03～0.05 Mpa; Insert the aerobic cultivation of adenylate cyclase gene engineering bacteria GD-002 20 ~ 30 hours in No. 2 jars, rotating speed 175r/min, throughput are 1m
3/ min, tank pressure 0.03～0.05 Mpa; In No. 1 fermentation tank, add 0.2% glucose and 0.1mmol/L IPTG induced 8 ~ 12 hours; In No. 2 fermentation tanks, add 0.2% glucose and 0.1mmol/L IPTG induced 8 ~ 12 hours;
(4) No. 1 jar zymotic fluid is carried out centrifugal treating, centrifuge speed is 16000r/min, charging rate is 300L/min, obtain bacterium mud, wash out bacterium mud, broken 30min in the ultrasonic cell-break machine with physiological saline, and then high speed centrifugation, centrifuge speed is 16000r/min, and charging rate is 200L/min, obtains supernatant 1; To carrying out centrifugal treating in No. 2 jar zymotic fluids, centrifuge speed is 8000r/min, charging rate is 200L/h, obtain bacterium mud, wash out bacterium mud, broken 30min in the ultrasonic cell-break machine with the TE buffer solution, and then high speed centrifugation, centrifuge speed is 8000r/min, and charging rate is 150L/h, obtains supernatant 2; Supernatant 1,2 is 40 purpose wheat bran mixing 10min with the fineness of 500kg, and mixture changes the oven dry charging tray over to, places in the air-dry cyclic drying case, and baking temperature is 45 ± 2 ℃, and dry 24h gets finished product.
Nutrition immune type aquatic attractant is made by following method:
(1) get the flesh of fish 200 weight portions, Mactra veneriformis meat 50 weight portions, running water 500 weight portions adopt rubber mill to carry out the glue mill 20 ~ 40 minutes, get glue mill liquid;
(2) adding NaOH solution in glue mill liquid, and stir, carry out the adjusting of acid-base value with NaOH solution, is 7.0 up to the pH value;
(3) 120 orders that sieve are removed large granular materials, and the liquid material that will remove big material adds in the enzymatic vessel; Be warmed to 60 ℃ then, constantly stir simultaneously;
(4) take by weighing trypsase 0.004 weight portion of 6000IU/G, papain 0.004 weight portion, the water dissolving is added in the enzymatic vessel, constantly stirs enzymolysis 3 hours in the enzymolysis process; Be warmed up to 88 ℃, kept 15 minutes, cool to 60 ℃ again; Use the Aminopeptidase P enzymolysis again 5 hours, and removed the bitter taste in the material;
(5) add following composition again: 0.6 weight portion adenosine diphosphate, 0.25 weight portion DMPT, 0.2 weight portion glutathione, 0.4 weight portion betaine, stir, on air-dry drying machine, carry out drying, make final moisture content less than 12%, get finished product.
The fermentation protein peptide of the growth of this product utilization gene recombinant protein immunopotentiating agent, nutrition immune type aquatic attractant and high antibacterial peptide content is the substitute products of novel feeding antibiotic.Use the young crab safe feed of three kinds of Additive Production, has performance safe, efficient, environmental protection, can effectively suppress multiple pathogenic entero becteria, improve the digestibility of feed nutrient, promote the growth of crab, and can strengthen the crab immunity of organisms, significantly improve young crab survival rate (reaching more than 80%); Do not use antibiotic in the feed, the feed drug residue is zero.
The invention will be further described below in conjunction with embodiment:
Embodiment 1:
A kind of young crab safe compound feed, form by weight components:
Dregs of beans 15.9%
The cottonseed dregs of rice 6%
The dish dregs of rice 26.5%
Peanut meal 10%
Fish meal 3.5%
Aquatic products fermentation protein peptide 8%
Cuttlefish cream 2.5%
Shrimp head powder 2.5%
Phosphatide oil 2%
Fish oil 1.5%
Calcium dihydrogen phosphate 1.5%
Flour 18%
Shelling plain 0.1%
Crab is with micro-1%
Crab is used B B-complex 0.2%
50% Choline Chloride 0.2%
Gene recombinant protein 0.1%
Nutrition immune type aquatic attractant 0.5%;
Crab is the mixture of B B-complex and carrier with B B-complex, and carrier is a time powder, and per 1000 gram crabs are with containing vitamin A 10000000IU, vitamin D in the B B-complex
32000000IU, vitamin E 75000IU, vitamin K
37500mg, Cobastab
120000mg, Cobastab
225000mg, Cobastab
620000mg, Cobastab
1220mg, nicotinic acid 60000mg, calcium pantothenate 25000mg, folic acid 2500mg, biotin 200mg, vitamin C 150000mg, inositol 100000mg;
Crab uses trace mineral supplement by the mixture of trace element with carrier, carrier is a zeolite, and per 1000 gram crabs are with containing iron 15000mg, manganese 3000mg, copper 2000mg, zinc 6000mg, cobalt 500mg, iodine 1000mg, selenium 100mg, magnesium 50000mg, potassium 25000mg in the trace mineral supplement.
Described aquatic products is made by following method with the fermentation protein peptide:
(1) configuration bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus fermenti are used for ferment tank and cultivate with four kinds of culture mediums; Wherein the bacillus subtilis culture medium prescription is: peptone 1wt%, and yeast extract 0.5 wt %, sodium chloride 0.5 wt %, dipotassium hydrogen phosphate 0.5 wt %, all the other are water; The Yeast Cultivation based formulas is: brewer's wort 20 wt %, and all the other are water; The culture medium prescription of streptococcus lactis and lactobacillus fermenti is: peptone 10 weight portions, powdered beef 5 weight portions, dusty yeast 4 weight portions, glucose 20 weight portions, Tween 80 1 weight portion dipotassium hydrogen phosphate 2 weight portions, sodium acetate 5 weight portions, Triammonium citrate 2 weight portion magnesium sulfate 0.2 weight portion, manganese sulfate 0.05 weight portion, agar powder 15 weight portions, distilled water 1000 weight portions; Above-mentioned each culture medium is all through 121 ℃ of autoclaving 15 ~ 20min;
(2) fermentation tank sterilization, inoculate after the cooling: respectively from jar mouth flame method, insert streptococcus lactis, lactobacillus fermenti in 1, No. 2 jar, carry out anaerobism and cultivate, each hour stirs once culture medium in the tank body; Respectively at inserting saccharomycete, bacillus subtilis in 3, No. 4 jars, carry out aerobic cultivation, rotating speed 175r/min, throughput are 0.8m
3/ min, tank pressure 0.03～0.05 Mpa;
(3) cultivating after fermentation in 12 ~ 24 hours finishes.Emitting bacterium liquid by measuring tank from four fermentation tanks mixes in blending tank, mixed bacteria liquid is stored in the receiver, feeds bacterium liquid from receiver in solid blender, and bacterium liquid is mixed with dregs of beans, mixed proportion is 1:2, and incorporation time is 5 minutes;
(4) the even accumulation of mixed material is distributed in ground between solid fermentation, highly is 0.6 meter, loam cake film, sealing all around.Carried out anaerobic fermentation 100 ~ 150 hours; Material after will fermenting again carries out 30 ~ 80 ℃ of low temperature dryings, pulverizing obtains aquatic products fermentation protein peptide.
Described gene recombinant protein is made by following method:
(1) in fermentation tank, disposes IGF respectively
Genetic engineering bacterium GD-001 and adenylate cyclase gene engineering bacteria GD-002 culture medium: IGF
Genetic engineering bacterium GD-001 and adenylate cyclase gene engineering bacteria GD-002 culture medium prescription are: peptone 1wt%, and yeast extract 0.5wt%, sodium chloride 0.5%, dipotassium hydrogen phosphate 0.5wt%, all the other are water; Culture medium is handled through 121 ℃ of autoclaving 20min;
(2) each adds the edible oil that is equivalent to culture medium weight 0.02 ~ 0.05% in fermentation, to eliminate barm;
(3) to fermentation tank culture medium sterilization and cooling, inoculate then: in No. 1 jar, insert IGF
The aerobic cultivation of genetic engineering bacterium GD-001 20 ~ 30 hours, rotating speed 175r/min, throughput are 1m
3/ min, tank pressure 0.03～0.05 Mpa; Insert the aerobic cultivation of adenylate cyclase gene engineering bacteria GD-002 20 ~ 30 hours in No. 2 jars, rotating speed 175r/min, throughput are 1m
3/ min, tank pressure 0.03～0.05 Mpa; In No. 1 fermentation tank, add 0.2% glucose and 0.1mmol/L IPTG induced 8 ~ 12 hours; In No. 2 fermentation tanks, add 0.2% glucose and 0.1mmol/L IPTG induced 8 ~ 12 hours;
(4) No. 1 jar zymotic fluid is carried out centrifugal treating, centrifuge speed is 16000r/min, charging rate is 300L/min, obtain bacterium mud, wash out bacterium mud, broken 30min in the ultrasonic cell-break machine with physiological saline, and then high speed centrifugation, centrifuge speed is 16000r/min, and charging rate is 200L/min, obtains supernatant 1; To carrying out centrifugal treating in No. 2 jar zymotic fluids, centrifuge speed is 8000r/min, charging rate is 200L/h, obtain bacterium mud, wash out bacterium mud, broken 30min in the ultrasonic cell-break machine with the TE buffer solution, and then high speed centrifugation, centrifuge speed is 8000r/min, and charging rate is 150L/h, obtains supernatant 2; Supernatant 1,2 is 40 purpose wheat bran mixing 10min with the fineness of 500kg, and mixture changes the oven dry charging tray over to, places in the air-dry cyclic drying case, and baking temperature is 45 ± 2 ℃, and dry 24h gets finished product.
Nutrition immune type aquatic attractant is made by following method:
(1) get the flesh of fish 200 weight portions, Mactra veneriformis meat 50 weight portions, running water 500 weight portions adopt rubber mill to carry out the glue mill 20 ~ 40 minutes, get glue mill liquid;
(2) adding NaOH solution in glue mill liquid, and stir, carry out the adjusting of acid-base value with NaOH solution, is 7.0 up to the pH value;
(3) 120 orders that sieve are removed large granular materials, and the liquid material that will remove big material adds in the enzymatic vessel; Be warmed to 60 ℃ then, constantly stir simultaneously;
(4) take by weighing trypsase 0.004 weight portion of 6000IU/G, papain 0.004 weight portion, the water dissolving is added in the enzymatic vessel, constantly stirs enzymolysis 3 hours in the enzymolysis process; Be warmed up to 88 ℃, kept 15 minutes, cool to 60 ℃ again; Use the Aminopeptidase P enzymolysis again 5 hours, and removed the bitter taste in the material;
(5) add following composition again: 0.6 weight portion adenosine diphosphate, 0.25 weight portion DMPT, 0.2 weight portion glutathione, 0.4 weight portion betaine, stir, on air-dry drying machine, carry out drying, make final moisture content less than 12%, get finished product.
Embodiment 2:
A kind of young crab safe compound feed, form by weight components:
Dregs of beans 18%
The cottonseed dregs of rice 7%
The dish dregs of rice 23.3%
Peanut meal 9%
Fish meal 3%
Aquatic products fermentation protein peptide 7%
Cuttlefish cream 3%
Shrimp head powder 2%
Phosphatide oil 1%
Fish oil 2%
Calcium dihydrogen phosphate 2%
Flour 20%
Shelling plain 0.2%
Crab is with micro-1.5%
Crab is used B B-complex 0.3%
50% Choline Chloride 0.1%
Gene recombinant protein 0.2%
Nutrition immune type aquatic attractant 0.4%;
All the other are with embodiment 1.
Claims (4)
1. young crab safe compound feed is characterized in that: be made up of weight components:
Dregs of beans 15 ~ 20%
The cottonseed dregs of rice 6 ~ 7%
The dish dregs of rice 20 ~ 30%
Peanut meal 8 ~ 12%
Fish meal 3 ~ 5%
Aquatic products fermentation protein peptide 5 ~ 9%
Cuttlefish cream 2 ~ 3%
Shrimp head powder 2 ~ 3%
Phosphatide oil 1 ~ 3%
Fish oil 1 ~ 2%
Calcium dihydrogen phosphate 1 ~ 2%
Flour 15 ~ 20%
Shelling plain 0.05 ~ 0.2%
Crab is with micro-0.5 ~ 1.5%
Crab is used B B-complex 0.1 ~ 0.3%
50% Choline Chloride 0.1 ~ 0.3%
Gene recombinant protein 0.05 ~ 0.2%
Nutrition immune type aquatic attractant 0.4 ~ 0.6%
Above-mentioned each amounts of components sum is 100%;
Crab is the mixture of B B-complex and carrier with B B-complex, and carrier is a time powder, and per 1000 gram crabs are with containing vitamin A 10000000IU, vitamin D in the B B-complex
32000000IU, vitamin E 75000IU, vitamin K
37500mg, Cobastab
120000mg, Cobastab
225000mg, Cobastab
620000mg, Cobastab
1220mg, nicotinic acid 60000mg, calcium pantothenate 25000mg, folic acid 2500mg, biotin 200mg, vitamin C 150000mg, inositol 100000mg;
Crab uses trace mineral supplement by the mixture of trace element with carrier, carrier is a zeolite, and per 1000 gram crabs are with containing iron 15000mg, manganese 3000mg, copper 2000mg, zinc 6000mg, cobalt 500mg, iodine 1000mg, selenium 100mg, magnesium 50000mg, potassium 25000mg in the trace mineral supplement.
2. young crab safe compound feed according to claim 1, it is characterized in that: described aquatic products is made by following method with the fermentation protein peptide:
(1) configuration bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus fermenti are used for ferment tank and cultivate with four kinds of culture mediums; Wherein the bacillus subtilis culture medium prescription is: peptone 1wt%, and yeast extract 0.5 wt %, sodium chloride 0.5 wt %, dipotassium hydrogen phosphate 0.5 wt %, all the other are water; The Yeast Cultivation based formulas is: brewer's wort 20 wt %, and all the other are water; The culture medium prescription of streptococcus lactis and lactobacillus fermenti is: peptone 10 weight portions, powdered beef 5 weight portions, dusty yeast 4 weight portions, glucose 20 weight portions, Tween 80 1 weight portion dipotassium hydrogen phosphate 2 weight portions, sodium acetate 5 weight portions, Triammonium citrate 2 weight portion magnesium sulfate 0.2 weight portion, manganese sulfate 0.05 weight portion, agar powder 15 weight portions, distilled water 1000 weight portions; Above-mentioned each culture medium is all through 121 ℃ of autoclaving 15 ~ 20min;
(2) fermentation tank sterilization, inoculate after the cooling: respectively from jar mouth flame method, insert streptococcus lactis, lactobacillus fermenti in 1, No. 2 jar, carry out anaerobism and cultivate, each hour stirs once culture medium in the tank body; Respectively at inserting saccharomycete, bacillus subtilis in 3, No. 4 jars, carry out aerobic cultivation, rotating speed 175r/min, throughput are 0.8m
3/ min, tank pressure 0.03～0.05 Mpa;
(3) cultivating after fermentation in 12 ~ 24 hours finishes, emitting bacterium liquid by measuring tank from four fermentation tanks mixes in blending tank, mixed bacteria liquid is stored in the receiver, from receiver, feed bacterium liquid in solid blender, bacterium liquid is mixed with dregs of beans, mixed proportion is 1:2, and incorporation time is 5 minutes;
(4) the even accumulation of mixed material is distributed in ground between solid fermentation, highly is 0.6 meter, the loam cake film, sealing was carried out anaerobic fermentation 100 ~ 150 hours all around; Material after will fermenting again carries out 30 ~ 80 ℃ of low temperature dryings, pulverizing obtains aquatic products fermentation protein peptide.
3. young crab safe compound feed according to claim 1, it is characterized in that: described gene recombinant protein is made by following method:
(1) in fermentation tank, disposes IGF respectively
Genetic engineering bacterium GD-001 and adenylate cyclase gene engineering bacteria GD-002 culture medium: IGF
Genetic engineering bacterium GD-001 and adenylate cyclase gene engineering bacteria GD-002 culture medium prescription are: peptone 1wt%, and yeast extract 0.5wt%, sodium chloride 0.5%, dipotassium hydrogen phosphate 0.5wt%, all the other are water; Culture medium is handled through 121 ℃ of autoclaving 20min;
(2) each adds the edible oil that is equivalent to culture medium weight 0.02 ~ 0.05% in fermentation, to eliminate barm;
(3) to fermentation tank culture medium sterilization and cooling, inoculate then: in No. 1 jar, insert IGF
The aerobic cultivation of genetic engineering bacterium GD-001 20 ~ 30 hours, rotating speed 175r/min, throughput are 1m
3/ min, tank pressure 0.03～0.05 Mpa; Insert the aerobic cultivation of adenylate cyclase gene engineering bacteria GD-002 20 ~ 30 hours in No. 2 jars, rotating speed 175r/min, throughput are 1m
3/ min, tank pressure 0.03～0.05 Mpa; In No. 1 fermentation tank, add 0.2% glucose and 0.1mmol/L IPTG induced 8 ~ 12 hours; In No. 2 fermentation tanks, add 0.2% glucose and 0.1mmol/L IPTG induced 8 ~ 12 hours;
(4) No. 1 jar zymotic fluid is carried out centrifugal treating, centrifuge speed is 16000r/min, charging rate is 300L/min, obtain bacterium mud, wash out bacterium mud, broken 30min in the ultrasonic cell-break machine with physiological saline, and then high speed centrifugation, centrifuge speed is 16000r/min, and charging rate is 200L/min, obtains supernatant 1; To carrying out centrifugal treating in No. 2 jar zymotic fluids, centrifuge speed is 8000r/min, charging rate is 200L/h, obtain bacterium mud, wash out bacterium mud, broken 30min in the ultrasonic cell-break machine with the TE buffer solution, and then high speed centrifugation, centrifuge speed is 8000r/min, and charging rate is 150L/h, obtains supernatant 2; Supernatant 1,2 is 40 purpose wheat bran mixing 10min with the fineness of 500kg, and mixture changes the oven dry charging tray over to, places in the air-dry cyclic drying case, and baking temperature is 45 ± 2 ℃, and dry 24h gets finished product.
4. young crab safe compound feed according to claim 1 is characterized in that: nutrition immune type aquatic attractant is made by following method:
(1) get the flesh of fish 200 weight portions, Mactra veneriformis meat 50 weight portions, running water 500 weight portions adopt rubber mill to carry out the glue mill 20 ~ 40 minutes, get glue mill liquid;
(2) adding NaOH solution in glue mill liquid, and stir, carry out the adjusting of acid-base value with NaOH solution, is 7.0 up to the pH value;
(3) 120 orders that sieve are removed large granular materials, and the liquid material that will remove big material adds in the enzymatic vessel; Be warmed to 60 ℃ then, constantly stir simultaneously;
(4) take by weighing trypsase 0.004 weight portion of 6000IU/G, papain 0.004 weight portion, the water dissolving is added in the enzymatic vessel, constantly stirs enzymolysis 3 hours in the enzymolysis process; Be warmed up to 88 ℃, kept 15 minutes, cool to 60 ℃ again; Use the Aminopeptidase P enzymolysis again 5 hours, and removed the bitter taste in the material;
(5) add following composition again: 0.6 weight portion adenosine diphosphate, 0.25 weight portion DMPT, 0.2 weight portion glutathione, 0.4 weight portion betaine, stir, on air-dry drying machine, carry out drying, make final moisture content less than 12%, get finished product.
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| CN2011100873589A CN102125193A (en) | 2011-04-08 | 2011-04-08 | Safe compound feed for crablets |
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| CN2011100873589A CN102125193A (en) | 2011-04-08 | 2011-04-08 | Safe compound feed for crablets |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613423A (en) * | 2012-04-19 | 2012-08-01 | 西南大学 | Microelement premix for river crabs |
| CN102640866A (en) * | 2012-04-20 | 2012-08-22 | 南通巴大饲料有限公司 | Safe compound feed for allogynogenetic crucian carp |
| CN103330086A (en) * | 2013-07-09 | 2013-10-02 | 句容市三岔蟹业专业合作社 | Juvenile river crab feed and preparation method thereof |
| CN103478439A (en) * | 2012-06-07 | 2014-01-01 | J&B生物株式会社 | Additive composition for shrimp feed and a method for producing the same |
| CN103504144A (en) * | 2013-09-03 | 2014-01-15 | 陈秀苓 | Larva crab feed |
| CN103504171A (en) * | 2013-10-17 | 2014-01-15 | 丹阳市正大油脂有限公司 | Juvenile crab feed |
| CN103636943A (en) * | 2013-11-26 | 2014-03-19 | 大连创达技术交易市场有限公司 | Feed additive |
| CN103734490A (en) * | 2013-11-20 | 2014-04-23 | 青岛佰众化工技术有限公司 | Portunus trituberculatus larva cultivation forage |
| CN104920903A (en) * | 2015-07-07 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Crab fodder with added enteromorpha prolifera and Chinese herbal medicine immunopotentiator |
| CN106562106A (en) * | 2016-11-01 | 2017-04-19 | 广西大学 | River crab fattening feed |
| CN107183352A (en) * | 2017-07-13 | 2017-09-22 | 陕西科技大学 | A kind of preparation technology of antibacterial peptide biological feedstuff |
| CN107616298A (en) * | 2017-09-29 | 2018-01-23 | 明光市永言水产食品有限公司 | One kind improves young crab survival rate nutrient fodder |
| CN107996882A (en) * | 2017-12-13 | 2018-05-08 | 无锡华诺威动物保健品有限公司 | One seed shrimp crab special feed premix and its preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569376A (en) * | 2009-06-01 | 2009-11-04 | 浙江金大地饲料有限公司 | Expanded sinking formula feed suitable for sea crabs to ingest and method for preparing same |
-
2011
- 2011-04-08 CN CN2011100873589A patent/CN102125193A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569376A (en) * | 2009-06-01 | 2009-11-04 | 浙江金大地饲料有限公司 | Expanded sinking formula feed suitable for sea crabs to ingest and method for preparing same |
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| CN102613423A (en) * | 2012-04-19 | 2012-08-01 | 西南大学 | Microelement premix for river crabs |
| CN102613423B (en) * | 2012-04-19 | 2013-07-03 | 西南大学 | Microelement premix for river crabs |
| CN102640866A (en) * | 2012-04-20 | 2012-08-22 | 南通巴大饲料有限公司 | Safe compound feed for allogynogenetic crucian carp |
| CN103478439A (en) * | 2012-06-07 | 2014-01-01 | J&B生物株式会社 | Additive composition for shrimp feed and a method for producing the same |
| CN103478439B (en) * | 2012-06-07 | 2015-09-02 | Jb生物株式会社 | Shrimp feed addictive composition and manufacture method thereof |
| CN103330086A (en) * | 2013-07-09 | 2013-10-02 | 句容市三岔蟹业专业合作社 | Juvenile river crab feed and preparation method thereof |
| CN103504144A (en) * | 2013-09-03 | 2014-01-15 | 陈秀苓 | Larva crab feed |
| CN103504171A (en) * | 2013-10-17 | 2014-01-15 | 丹阳市正大油脂有限公司 | Juvenile crab feed |
| CN103734490A (en) * | 2013-11-20 | 2014-04-23 | 青岛佰众化工技术有限公司 | Portunus trituberculatus larva cultivation forage |
| CN103734490B (en) * | 2013-11-20 | 2015-10-21 | 青岛佰众化工技术有限公司 | A kind of Crab Portunus trituberculatus Larvae cultivates feed |
| CN103636943A (en) * | 2013-11-26 | 2014-03-19 | 大连创达技术交易市场有限公司 | Feed additive |
| CN104920903A (en) * | 2015-07-07 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Crab fodder with added enteromorpha prolifera and Chinese herbal medicine immunopotentiator |
| CN106562106A (en) * | 2016-11-01 | 2017-04-19 | 广西大学 | River crab fattening feed |
| CN107183352A (en) * | 2017-07-13 | 2017-09-22 | 陕西科技大学 | A kind of preparation technology of antibacterial peptide biological feedstuff |
| CN107616298A (en) * | 2017-09-29 | 2018-01-23 | 明光市永言水产食品有限公司 | One kind improves young crab survival rate nutrient fodder |
| CN107996882A (en) * | 2017-12-13 | 2018-05-08 | 无锡华诺威动物保健品有限公司 | One seed shrimp crab special feed premix and its preparation method and application |
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