CN102102051A - Alkaline cleaning solution applied to automatic biochemical analyzer - Google Patents
Alkaline cleaning solution applied to automatic biochemical analyzer Download PDFInfo
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- CN102102051A CN102102051A CN 201010584650 CN201010584650A CN102102051A CN 102102051 A CN102102051 A CN 102102051A CN 201010584650 CN201010584650 CN 201010584650 CN 201010584650 A CN201010584650 A CN 201010584650A CN 102102051 A CN102102051 A CN 102102051A
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- alkaline cleaning
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Abstract
The invention discloses an alkaline cleaning solution applied to an automatic biochemical analyzer. The alkaline cleaning solution comprises a quaternary ammonium salt cationic surfactant, a non-ionic surfactant, alkali metal hydroxide, a metal chelate and an antifoaming agent. The alkaline cleaning solution has an excellent cleaning effect, low cost, high stability, low residual rate of substances after cleaning and particularly low residual rate of metal hydroxide.
Description
Technical field
The present invention relates to a kind of scavenging solution, specifically relate to the alkaline cleaning fluid that a kind of automatic biochemistry analyzer uses when analytical specimen and routine maintenance.
Background technology
Automatic biochemistry analyzer is characteristics with high accuracy, high degree of accuracy, high flexibility and high efficiency, is bearing a large amount of work in clinical labororatory, is one of instrument of being most widely used of present clinical labororatory.In clinical labororatory, need the project of detection many on the one hand, the external diagnosis reagent composition complexity of Shi Yonging correspondingly, specimen types is various on the other hand, serum, blood plasma, urine, ascites pleural fluid, cerebrospinal fluid etc. are arranged, and therefore, reaction cup, pipeline etc. just might be by pollutions such as scleroproein, crystallization, grease, dyestuffs, this shows that cleaning performance can directly influence the accuracy of detected result.In order to obtain reliable result, it is particularly important that the cleaning system of automatic biochemistry analyzer just seems.Scavenging solution is one of topmost component in the cleaning system, and it is related to the problem of cleaning performance, and the special-purpose scavenging solution of some Biochemical Analyzers mostly is an alkaline cleaning fluid on the market.
The alkaline cleaning fluid source of clinical labororatory's use at present has two: a source is to use the alkaline cleaning fluid of import, but the import reagent cost is higher, makes that detecting cost increases, so its widespread use is limited; The another one source is to use the home-made alkaline cleaning fluid, but the actual cleaning performance of homemade alkaline cleaning fluid is not good enough, poor stability.The cleaning performance of homemade alkaline cleaning fluid is not good be meant the cleaning of inorganic componentss such as organism such as protein, lipid material and inorganic salt unclean, especially not thoroughly or do not add processing to the processing of metal ion.We know that some contains metal ion the external diagnosis reagent that uses on the automatic biochemistry analyzer, and divalent-metal ion particularly is as Mg
2+, Cu
2+, Fe
2+, Ca
2+Deng, these metal ions can with the OH in the alkaline cleaning fluid
-Be formed on that major part is indissoluble or insoluble metal hydroxides in the water, these indissolubles or insolubles very easily stick on the reaction wall of cup and on the waste liquid tube wall.If the cleaning to protein matter, lipid material, reactant precipitation and metal hydroxides is unclean, then very easily reduces the precision and the accuracy of detected result, and cause crossed contamination.
Summary of the invention
Technical problem to be solved by this invention is the deficiency that overcomes above prior art problem, and a kind of alkaline cleaning fluid that is used for automatic biochemistry analyzer is provided, this alkaline cleaning fluid cleaning performance excellence, material is residual low, especially metal hydroxides is residual low, and cost is low, good stability.
For achieving the above object, the present invention has adopted following technical scheme:
The invention provides a kind of alkaline cleaning fluid that is used for automatic biochemistry analyzer, described scavenging solution comprises quaternary cationics, nonionogenic tenside, alkali metal hydroxide, metallo-chelate and defoamer.
Described each composition content in alkaline cleaning fluid is: the quaternary cationics volume is 0.005～0.02% of an alkaline cleaning fluid volume, the nonionogenic tenside volume is 0.1～3.2% of an alkaline cleaning fluid volume, alkali metal hydroxide weight is 20～40g/L, metallo-chelate weight is 19～37g/L, the defoamer volume is 0.8～2.0% of an alkaline cleaning fluid volume, more preferably the quaternary cationics volume is 0.005% of an alkaline cleaning fluid volume, the nonionogenic tenside volume is 0.1% of an alkaline cleaning fluid volume, alkali metal hydroxide weight is 20g/L, metallo-chelate weight is 19g/L, and the defoamer volume is 0.8% of an alkaline cleaning fluid volume.Concentration is crossed low or too high, all may cause cleaning not thorough, does not reach requirement.
Described quaternary cationics is that bromine alkyl dimethyl is for benzene methanamine (be commonly called as and be bromogeramine) or dodecyl-dimethyl-2-phenoxy group-ethyl brometo de amonio (be commonly called as and be domiphen bromide) or tetradecyl-lutidine brometo de amonio (be commonly called as and be Myristylpicolinii Bromide).Quaternary cationics has acid-fast alkali-proof and abstersive character, plays wetting, emulsification, dispersion and cleaning function; Simultaneously it also has germicidal action, by the very strong adsorption of bacterium is adsorbed on the surface with bacterium, thereby stops breathing and the sugar of bacterium to separate effect, impels protein denaturation, plays sterilization effect.
Described nonionogenic tenside is that polyoxyethylene glycol is to iso-octyl phenyl ether (English name is Triton-X 100) or polyoxyethylene sorbitan monooleate (English name is Tween-80).Nonionogenic tenside has very strong surfactivity effect, plays the effect of an emulsification, cleaning in the present invention, assists organism such as protein remaining on cleaning reaction cup and the tube wall, lipid, organic ion.
Described alkali metal hydroxide is sodium hydroxide or potassium hydroxide.The main effect of alkali metal hydroxide is organism such as solubilising protein, lipid, reduces the viscosity of residuals, improves cleaning performance.
Described metallo-chelate is disodium ethylene diamine tetraacetate (English name is EDTA-2Na).Disodium ethylene diamine tetraacetate is a kind of amino carboxy chelating agent with amino and carboxyl, it contains 2 amino nitrogens and 4 carboxyl oxygens, can cooperate with most metal ions, generate the very high inner complex of stability, because formed inner complex is electrically charged mostly, very easily water-soluble, therefore can effectively prevent the insoluble precipitate metal hydroxides and pollute reaction cup.
Described defoamer is a Virahol.Virahol can be eliminated the bubble that produces when reaction cup is rotated effectively as defoamer.
Compared with prior art, the present invention has following remarkable advantage and beneficial effect:
Alkaline cleaning fluid is by quaternary cationics, nonionogenic tenside, alkali metal hydroxide, metallo-chelate and defoamer are formulated by a certain percentage, used the combination of nonionic and cationic tensio-active agent in the composition, while auxiliary alkali metal hydroxides and defoamer, the alkaline cleaning fluid of making is to protein, inorganic componentss such as organism such as lipid material and inorganic salt all have better cleaning effect, contain the metallo-chelate disodium ethylene diamine tetraacetate in addition in the alkaline cleaning fluid, it effectively chelated metal ions form water miscible inner complex, thereby the generation that has effectively prevented the insoluble precipitate metal hydroxides has realized that fully low metal hydroxides is residual.Alkaline cleaning fluid placed after the enough time do the contrast experiment with the alkaline cleaning fluid of new preparation, the result shows that two groups of results do not have significant difference, and dependency is good, and this illustrates that this alkaline cleaning fluid has good stability.Because alkaline cleaning fluid of the present invention is formulated, the preparation agents useful for same is commercially available general industry product, so effectively reduce cost.
Embodiment
Below in conjunction with embodiment the present invention is further described in detail, but is not limited to this.
Embodiment 1:
Weigh in the balance and get sodium hydroxide 20g, EDTA-2Na 19g, place the 1000ml beaker, add entry 500-700mL and make dissolving fully, slowly add Triton-X 1001mL again, Virahol 8mL, bromogeramine (1%) 5mL, stirring is fully dissolved it, adds water with graduated cylinder at last and is settled to 1L, and promptly obtaining volume is the automatic biochemistry analyzer alkaline cleaning fluid of 1L.
Embodiment 2:
Weigh in the balance and get sodium hydroxide 40g, EDTA-2Na 37g, place the 1000ml beaker, add entry 500-700mL and make dissolving fully, slowly add Triton-X 10032mL again, Virahol 20mL, bromogeramine (1%) 20mL, stirring is fully dissolved it, adds water with graduated cylinder at last and is settled to 1L, and promptly obtaining volume is the automatic biochemistry analyzer alkaline cleaning fluid of 1L.
Embodiment 3:
Weigh in the balance and get sodium hydroxide 30g, EDTA-2Na 26g, place the 1000ml beaker, add entry 500-700mL and make dissolving fully, slowly add Triton-X 10016.5mL again, Virahol 14mL, bromogeramine (1%) 12.5mL, stirring is fully dissolved it, adds water with graduated cylinder at last and is settled to 1L, and promptly obtaining volume is the automatic biochemistry analyzer alkaline cleaning fluid of 1L.
With the on-line cleaning performance that Hitachi's 7060 automatic biochemistry analyzers are tested above-mentioned 3 kinds of alkaline cleaning fluids respectively, behind the execution wash procedure, get low value Quality Control B available from Roche Holding Ag
1With high value Quality Control B
2Replication triglyceride level (TG), lipase (LPS), total protein (TP), creatinine (CREA), gpt (ALT), β-Qiang Dingsuan projects such as (β OHB) are respectively 20 times on Hitachi's 7060 automatic biochemistry analyzers, again in 20 days to repeatability between doing with a collection of Quality Control batch, test item is identical, the result shows that its repeatability is good, all is respectively CV≤1.75% and CV≤4.02% with betweenrun precision in batch.
After using alkaline cleaning fluid of the present invention, automatic biochemistry analyzer detected result precision is good, no crossed contamination; Before and after the test, the no significant difference of following glass of 340nm wavelength blank, it is residual to have a low material, and it is residual especially to hang down the insoluble metal hydroxides.
The alkaline cleaning fluid of preparation is divided into two equal portions, preserve 6 months respectively at 4 ℃ with 25 ℃ after, its outward appearance, pH value have no significant change.The alkaline cleaning fluid of preserving under 25 ℃ of temperature 6 months is done the contrast experiment with the alkaline cleaning fluid of preparation newly, test its on-line cleaning performance, behind the execution wash procedure, get low value Quality Control B available from Roche Holding Ag with Hitachi's 7060 automatic biochemistry analyzers
1With high value Quality Control B
2Projects such as replication TG, LPS, TP, CREA, ALT, β OHB are each 20 times on Hitachi's 7060 automatic biochemistry analyzers, and the result shows that two groups of results do not have significant difference (p＞0.05), dependency good (r＞0.995).Therefore, the alkaline cleaning fluid good stability that is used for automatic biochemistry analyzer provided by the invention did not have obvious influence to its result of use in 6 months to being less than under 25 ℃ of temperature to place.
The raw material that embodiment is used unless otherwise indicated, is the commercially available industrial goods that are fit to the reagent preparation.
Above-mentioned embodiment of the present invention is can not be used to limit the present invention to explanation of the present invention, and implication suitable with claims of the present invention and any change in the scope all should be thought to be included in the scope of claims.
Claims (8)
1. alkaline cleaning fluid that is used for automatic biochemistry analyzer, it is characterized in that: described scavenging solution comprises quaternary cationics, nonionogenic tenside, alkali metal hydroxide, metallo-chelate and defoamer.
2. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 1 is characterized in that: described each composition content in alkaline cleaning fluid is:
The quaternary cationics volume is 0.005～0.02% of an alkaline cleaning fluid volume;
The nonionogenic tenside volume is 0.1～3.2% of an alkaline cleaning fluid volume;
Alkali metal hydroxide weight is 20～40g/L;
Metallo-chelate weight is 19～37g/L;
The defoamer volume is 0.8～2.0% of an alkaline cleaning fluid volume.
3. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 2 is characterized in that: described each composition content in alkaline cleaning fluid is:
The quaternary cationics volume is 0.005% of an alkaline cleaning fluid volume;
The nonionogenic tenside volume is 0.1% of an alkaline cleaning fluid volume;
Alkali metal hydroxide weight is 20g/L;
Metallo-chelate weight is 19g/L;
The defoamer volume is 0.8% of an alkaline cleaning fluid volume.
4. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 1 is characterized in that: described quaternary cationics is that bromine alkyl dimethyl is for benzene methanamine or dodecyl-dimethyl-2-phenoxy group-ethyl brometo de amonio or tetradecyl-lutidine brometo de amonio.
5. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 1 is characterized in that: described nonionogenic tenside is that polyoxyethylene glycol is to iso-octyl phenyl ether or polyoxyethylene sorbitan monooleate.
6. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 1 is characterized in that: described alkali metal hydroxide is sodium hydroxide or potassium hydroxide.
7. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 1 is characterized in that: described metallo-chelate is a disodium ethylene diamine tetraacetate.
8. the alkaline cleaning fluid that is used for automatic biochemistry analyzer according to claim 1 is characterized in that: described defoamer is a Virahol.
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| CN 201010584650 CN102102051A (en) | 2010-12-01 | 2010-12-01 | Alkaline cleaning solution applied to automatic biochemical analyzer |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304439A (en) * | 2011-08-08 | 2012-01-04 | 宁波美康盛德生物科技有限公司 | Antibacterial cleaning solution for full-automatic biochemistry analyzer |
| CN103412088A (en) * | 2013-07-26 | 2013-11-27 | 北京控制工程研究所 | Chemical test method for bipropellant thruster after thermal standard cleaning |
| CN104804889A (en) * | 2015-03-06 | 2015-07-29 | 广州锐为生物科技有限公司 | Water-based foam cleaning agent |
| CN106753845A (en) * | 2016-11-30 | 2017-05-31 | 刘相东 | A kind of cleaning agent for blood analyser and preparation method thereof |
| CN106753876A (en) * | 2015-11-23 | 2017-05-31 | 迈瑞南京生物技术有限公司 | Bottle-washing agent |
| CN107365639A (en) * | 2017-03-31 | 2017-11-21 | 上海艾瑞德生物科技有限公司 | A kind of collaurum platform cleaning fluid |
| CN107557180A (en) * | 2017-09-26 | 2018-01-09 | 重庆赞龙生物科技有限公司 | A kind of cleaning fluid |
| CN108531310A (en) * | 2018-05-21 | 2018-09-14 | 苏州佑君环境科技有限公司 | A kind of chemo-immunity light-emitting appearance probe cleaning solution and preparation method thereof |
| CN108913376A (en) * | 2018-05-30 | 2018-11-30 | 武汉华大医学检验所有限公司 | A kind of reagent and its preparation method and application for sequence testing chip cleaning |
| CN109679769A (en) * | 2018-12-14 | 2019-04-26 | 湖南品信生物工程有限公司 | A kind of excrement cleaning solution for excrement detector |
| CN111896731A (en) * | 2020-06-24 | 2020-11-06 | 宁波瑞源生物科技有限公司 | Cleaning solution for chemiluminescence immunoassay |
| CN115368975A (en) * | 2022-08-03 | 2022-11-22 | 吉林基蛋生物科技有限公司 | Alkaline cleaning fluid and preparation method thereof |
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| CN1566304A (en) * | 2003-06-13 | 2005-01-19 | 李小平 | Full automatic biochemical analyzer cleaning agent |
| CN101470123A (en) * | 2007-12-26 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Cleaning fluid used for automatic biochemical analyzer |
| CN101492632A (en) * | 2009-03-02 | 2009-07-29 | 上海九誉生物科技有限公司 | Composite disinfection detergent |
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| CN1566304A (en) * | 2003-06-13 | 2005-01-19 | 李小平 | Full automatic biochemical analyzer cleaning agent |
| CN101470123A (en) * | 2007-12-26 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Cleaning fluid used for automatic biochemical analyzer |
| CN101492632A (en) * | 2009-03-02 | 2009-07-29 | 上海九誉生物科技有限公司 | Composite disinfection detergent |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304439A (en) * | 2011-08-08 | 2012-01-04 | 宁波美康盛德生物科技有限公司 | Antibacterial cleaning solution for full-automatic biochemistry analyzer |
| CN102304439B (en) * | 2011-08-08 | 2013-04-17 | 宁波美康盛德医学检验所有限公司 | Antibacterial cleaning solution for full-automatic biochemistry analyzer |
| CN103412088A (en) * | 2013-07-26 | 2013-11-27 | 北京控制工程研究所 | Chemical test method for bipropellant thruster after thermal standard cleaning |
| CN103412088B (en) * | 2013-07-26 | 2015-05-27 | 北京控制工程研究所 | Chemical test method for bipropellant thruster after thermal standard cleaning |
| CN104804889A (en) * | 2015-03-06 | 2015-07-29 | 广州锐为生物科技有限公司 | Water-based foam cleaning agent |
| CN106753876A (en) * | 2015-11-23 | 2017-05-31 | 迈瑞南京生物技术有限公司 | Bottle-washing agent |
| CN106753845A (en) * | 2016-11-30 | 2017-05-31 | 刘相东 | A kind of cleaning agent for blood analyser and preparation method thereof |
| CN107365639A (en) * | 2017-03-31 | 2017-11-21 | 上海艾瑞德生物科技有限公司 | A kind of collaurum platform cleaning fluid |
| CN107365639B (en) * | 2017-03-31 | 2020-04-24 | 上海艾瑞德生物科技有限公司 | Colloidal gold platform cleaning solution |
| CN107557180A (en) * | 2017-09-26 | 2018-01-09 | 重庆赞龙生物科技有限公司 | A kind of cleaning fluid |
| CN108531310A (en) * | 2018-05-21 | 2018-09-14 | 苏州佑君环境科技有限公司 | A kind of chemo-immunity light-emitting appearance probe cleaning solution and preparation method thereof |
| CN108913376A (en) * | 2018-05-30 | 2018-11-30 | 武汉华大医学检验所有限公司 | A kind of reagent and its preparation method and application for sequence testing chip cleaning |
| CN109679769A (en) * | 2018-12-14 | 2019-04-26 | 湖南品信生物工程有限公司 | A kind of excrement cleaning solution for excrement detector |
| CN111896731A (en) * | 2020-06-24 | 2020-11-06 | 宁波瑞源生物科技有限公司 | Cleaning solution for chemiluminescence immunoassay |
| CN115368975A (en) * | 2022-08-03 | 2022-11-22 | 吉林基蛋生物科技有限公司 | Alkaline cleaning fluid and preparation method thereof |
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