CN108913376A - A kind of reagent and its preparation method and application for sequence testing chip cleaning - Google Patents
A kind of reagent and its preparation method and application for sequence testing chip cleaning Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
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- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/044—Hydroxides or bases
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
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- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2006—Monohydric alcohols
- C11D3/2027—Monohydric alcohols unsaturated
- C11D3/2031—Monohydric alcohols unsaturated fatty or with at least 8 carbon atoms in the alkenyl chain
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/33—Amino carboxylic acids
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/37—Polymers
- C11D3/3703—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C11D3/373—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds containing silicones
- C11D3/3738—Alkoxylated silicones
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Abstract
This application discloses a kind of reagents and its preparation method and application for sequence testing chip cleaning.The application is used for the reagent of sequence testing chip cleaning, includes lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide, EDTA, epoxidized soybean oil, polyether modified silicon oil, ethyl alcohol and pure water.The sequence testing chip cleaning reagent of the application, the pollution sources such as the greasy dirt for sequence testing chip design its component, and using various plants extract as active detergents, each component, which matches, in reagent effectively to clean sequence testing chip, remove the impurity such as its surface and oil contaminant, spot is not stayed after cleaning, is not hazed, and cleaning effect is good.Also, the reagent of the application, chloride and low aromatic hydrocarbon, main active ingredient are not plant extracts, environmentally friendly, safe and stable, are free of any halogen composition, therefore will not destroy ozone layer；A kind of dedicated cleaning reagent is provided for sequence testing chip.
Description
Technical field
This application involves sequence testing chips to clean field, more particularly to a kind of reagent and its system for sequence testing chip cleaning
Preparation Method and application.
Background technique
Sequence testing chip cleaning purpose be in order to ensure sequence testing chip is clean, pollution-free, and then ensure sequencing quality.At present
Still without special sequence testing chip cleaning reagent, also lack corresponding research and report.In practice, generally according to chip manufacturer
It is proposed with ethyl alcohol and isopropanol carries out chip cleaning.The effect of ethyl alcohol and isopropanol is dissolution removal organic matter, accelerates
Volatilization achievees the effect that sequence testing chip cleans.But isopropanol is more toxic, and has obvious anesthetic effect, to eye, respiratory tract
The irritating effect of mucous membrane, retina and optic nerve can be damaged；It is included in by international cancer research institution of the World Health Organization 3
In class carcinogenic substance inventory.Also, the cleaning effect of ethyl alcohol and isopropanol is bad, and is easy to decompose, volatilize, and especially temperature is inclined
Under high condition of storage, it may occur that decomposition causes cleaning agent to fail.
Therefore, it is necessary to a kind of new reagent for being exclusively used in sequence testing chip cleaning be researched and developed, to ensure cleaning effect and sequencing
Quality.
Summary of the invention
The purpose of the application is to provide a kind of new reagent cleaned for sequence testing chip and the preparation method of the reagent
And application.
To achieve the goals above, the application uses following technical scheme：
On the one hand the application discloses the reagent for sequence testing chip cleaning, include lemon alcohol, gleditsia sinensis extracting solution, hydroxide
Potassium, EDTA, epoxidized soybean oil, polyether modified silicon oil, ethyl alcohol and pure water.
It should be noted that the sequence testing chip cleaning reagent of the application, wherein lemon alcohol is alcohols material, nonpolarity point
Son can dissolve the greasy dirt on sequence testing chip surface, and it is nontoxic, smell is pure and fresh；Gleditsia sinensis extracting solution is extracted by raw material of gleditsia sinensis
Plant source surfactant can reduce the degree of absorption between dirt and object as surfactant, remove spot；Hydrogen
Sodium oxide molybdena can make grease hydrolysis at soluble glycerol and higher fatty acids sodium；EDTA can be with alkali metal, rare earth element and mistake
The stable water soluble complex of the formation such as metal is crossed, for removing metal ion；Epoxidized soybean oil and polyether modified silicon oil conduct
Stabilizer can improve the stability of solution, improve the thermal stability and photostability of the application sequence testing chip cleaning reagent；And second
Pure and mild pure water reconciles the above components dissolution together as basic solvent.
The application is used for the reagent of sequence testing chip cleaning, and chemical safety is stablized, not chloride and low aromatic hydrocarbon, master
Wanting active ingredient is the plant extracts such as lemon alcohol, gleditsia sinensis extracting solution, epoxidized soybean oil, environmentally friendly, safe and stable, without any
Halogen composition, therefore ozone layer will not be destroyed, practice confirms, is used for the reagent of sequence testing chip cleaning to sequencing core using the application
Piece is cleaned, and the impurity such as surface and oil contaminant can be effectively removed, and spot is not stayed after cleaning, is not hazed, and cleaning effect is good.In identical item
Under part, compared to general ethyl alcohol and isopropanol cleaning reagent, using the sequence testing chip cleaning reagent of the application, to sequencing core
The cleaning effect of piece is more preferable, therefore, can more ensure and improve sequencing quality；It is identical in a kind of implementation of the application
Sequencing library and sequencing approach, the cleaning of sequence testing chip is carried out using the application reagent, and the sequencing Q30 value and FP% of acquisition are all bright
Sequence testing chip that is aobvious to be higher than, being cleaned using ethyl alcohol and isopropanol.Wherein, it hazes and is primarily referred to as, formed after cleaning in chip surface
Droplet, these droplets will affect the focusing of the laser in sequencing procedure and take pictures.Therefore, the reagent as sequence testing chip cleaning,
It cannot haze after cleaning.
Preferably, the application is used for the reagent of sequence testing chip cleaning, by lemon alcohol, the 5%- of mass fraction 10%-25%
14% gleditsia sinensis extracting solution, the potassium hydroxide of 8%-20%, 0.3%-2% EDTA, 1-7% epoxidized soybean oil, 0.1-3%
Polyether modified silicon oil, 10%-20% ethyl alcohol and 23%-60% pure water composition.
Preferably, in a kind of implementation of the application, it is preferred that for the reagent of sequence testing chip cleaning, by quality point
The lemon alcohol of number 20%, 8% gleditsia sinensis extracting solution, 10% potassium hydroxide, 2% EDTA, 5% epoxidized soybean oil, 3%
Polyether modified silicon oil, 20% ethyl alcohol and 32% pure water composition.
It should be noted that the key of the application be it is big by lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide, EDTA, epoxy
Soya-bean oil, polyether modified silicon oil, ethyl alcohol and pure water composition sequence testing chip cleaning special agent can be with as the dosage of each component
It is adjusted according to the sequence testing chip situation specifically cleaned；This application provides one to be generally applicable to various sequence testing chip cleanings
Formula, i.e., by the lemon alcohol of mass fraction 10%-25%, the gleditsia sinensis extracting solution of 5%-14%, 8%-20% potassium hydroxide,
The epoxidized soybean oil of EDTA, 1%-7% of 0.3%-2%, the polyether modified silicon oil of 0.1%-3%, 10%-20% ethyl alcohol and
The reagent of the pure water composition of 23%-60%.Also, an optimal reference formula is provided, i.e., by the lemon of mass fraction 20%
Alcohol, 8% gleditsia sinensis extracting solution, 10% potassium hydroxide, 2% EDTA, 5% epoxidized soybean oil, 3% polyether-modified silicon
The reagent of oil, 20% ethyl alcohol and 32% pure water composition.
It is appreciated that first, the reagent of the application formula can satisfy conventional sequence testing chip cleaning, still, for it
Its purpose or in order to make the application reagent have other functions, in the sequence testing chip cleaning function for not influencing the application reagent
In the case where, other active ingredients or auxiliary ingredients can also be added, be not specifically limited herein.Second, the above specific dosage
Range or specific consumption proportion, the proportion with preferable cleaning effect only provided in a kind of implementation of the application, out
In other purposes, for example, save cost or cleaning effect require it is poor in the case where, each component can be in scope of the present application
It is adjusted, is not specifically limited herein in addition.
The another side of the application discloses preparation method of reagent thereof of the application for sequence testing chip cleaning, including into pure water
Be added lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide, EDTA, ethyl alcohol, it is closed to be stirred to react, then add epoxidized soybean oil and
Polyether modified silicon oil stirs evenly, that is, obtains the reagent for sequence testing chip cleaning.
It should be noted that in the preparation method of the application, first by lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide,
EDTA, ethyl alcohol are added in pure water, closed to be stirred to react；Wherein, the purpose of stirring is to dissolve each component sufficiently, and closed environment
It can prevent reagent in whipping process from volatilizing, to ensure the quality of production；Finally add epoxidized soybean oil and polyether-modified silicon
Oil improves the stability of solution, and the sequence testing chip cleaning reagent of preparation is made to have better thermal stability and photostability.
Preferably, in the preparation method of the application, the closed time being stirred to react is 0.5h-1h.
Preferably, in the preparation method of the application, the mixing time stirred evenly is 10min-20min.
The reagent for disclosing the application on one side again for sequence testing chip cleaning of the application is in preparation for glass or hardware
Application in the environment-friendly cleaning agent of cleaning.
It should be noted that the application is used for the reagent of sequence testing chip cleaning, it can effectively clean and degrease, because
This can be completely used for the cleaning of other materials, such as glass, hardware etc. other than for sequence testing chip cleaning, same to have
There are the characteristics such as environmentally friendly, safe and stable.
The application's discloses the environment-friendly cleaning agent cleaned for glass or hardware on one side again, includes in the environment-friendly cleaning agent
The application is used for the reagent of sequence testing chip cleaning.
It should be noted that the reagent due to the application for sequence testing chip cleaning can be equally used for glass or hardware is clear
It washes, it is therefore possible to use the reagent of the application prepares corresponding environment-friendly cleaning agent；It is appreciated that cleaned for glass or hardware,
Other active ingredients or auxiliary ingredients can also be added in the reagent of the application, can be easy to use or increased or increased
Powerful is not specifically limited herein.For example, foaming agent, Ke Yixiang can be made in the reagent of the application in order to facilitate using
The auxiliary ingredients for the increase foam formulations answered, are not specifically limited herein.
Due to using the technology described above, the beneficial effects of the present application are as follows：
The reagent of the application is designed its component for pollution sources such as the greasy dirts of sequence testing chip, and is extracted using various plants
Object is as active detergents, and each component is matched and can effectively be cleaned to sequence testing chip in reagent, removes its surface and oil contaminant
Equal impurity, do not stay spot, do not haze, cleaning effect is good after cleaning.Also, the reagent of the application, not chloride and low aromatic hydrocarbon,
Main active ingredient is plant extracts, environmentally friendly, safe and stable, is free of any halogen composition, therefore will not destroy ozone layer；
A kind of dedicated cleaning reagent is provided for sequence testing chip.
Specific embodiment
The ethyl alcohol and isopropanol for mainly using sequence testing chip recommended by the manufacturer the cleaning of sequence testing chip at present, it is not dedicated
Sequence testing chip cleaning reagent；But isopropanol exists using security risk, is international cancer research institution of World Health Organization name
The carcinogen of column, and the cleaning effect of ethyl alcohol and isopropanol is bad, under the condition of storage of temperature drift, it may occur that decompose
Cleaning agent is caused to fail.
Based on the above understanding, present inventor especially analyzes the succession of sequence testing chip, determines and surveys
The main pollution of sequence chip or residual include：The dust in chip production workshop, lubrication oil vapour, fugitive dust, Tris, NaCl salt from
The biological substances such as son and bacterial clump and virus.In this regard, the application especial manufacture can effectively dissolve the above residual of removal
The reagent of ingredient, for being cleaned to sequence testing chip, to improve cleaning effect；I.e. the application includes lemon alcohol, gleditsia sinensis extraction
Liquid, potassium hydroxide, EDTA, epoxidized soybean oil, polyether modified silicon oil, ethyl alcohol and pure water the reagent for sequence testing chip cleaning.
The application provides a kind of dedicated cleaning reagent for sequence testing chip, and has that cleaning effect is good, function under high-temperature storage conditions
The advantages that stabilizability is good, Environmental Safety.
The application is described in further detail below by specific embodiment.Following embodiment only to the application carry out into
One step explanation, should not be construed as the limitation to the application.
Embodiment one
The reagent for sequence testing chip cleaning of this example, lemon alcohol, 5%-14% including mass fraction 10%-25%
Gleditsia sinensis extracting solution, the potassium hydroxide of 8%-20%, the epoxidized soybean oil of EDTA, 1%-7% of 0.3%-2%, 0.1%-3%
The pure water of polyether modified silicon oil, the ethyl alcohol of 10%-20% and 23%-60%；This example is specifically prepared by the dosage of each component of table 1
The sequence testing chip cleaning reagent of multiple formulas, and test respectively these sequence testing chip cleaning reagents surface volatilization the time, thoroughly
The performances such as light rate and intensity A, as a comparison, while contrast test recommends cleaning reagent using pure water and Illumina
The surface volatilization time, light transmittance and intensity A performance, wherein Illumina recommends the i.e. isometric mixing of cleaning reagent
Ethyl alcohol and isopropanol.In addition, being provided with some same big using lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide, EDTA, epoxy
Soya-bean oil, polyether modified silicon oil, ethyl alcohol and pure water are as sequence testing chip cleaning reagent, but dosage of each component is not exclusively in the above dosage
For the formula of range as control, each test and comparative test are as shown in table 1.The lemon alcohol of this example has purchased from Ji'an City Rong Sheng plant
Limit company, gleditsia sinensis extracting solution are purchased from Guangzhou Zi Hui cosmetics Co., Ltd, and potassium hydroxide, EDTA and epoxidized soybean oil are purchased from upper
Hai Huiluo trade Co., Ltd, polyether modified silicon oil are purchased from Anhui Ai Yueta silicone oil Co., Ltd.
This example is used for the reagent of sequence testing chip cleaning, and preparation method is as follows：
The first step, clean mixing plant and by the ratio of table 1 preparation pure water, lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide,
EDTA, epoxidized soybean oil, polyether modified silicon oil, ethyl alcohol.
Second step injects pure water in clean mixing plant, at the same be put into lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide,
Start closed stirring after EDTA, ethyl alcohol.
Third step after closed stirring 1h, is stirred for after putting into epoxidized soybean oil and polyether modified silicon oil into mixing plant
Obtain within 10 minutes the reagent for sequence testing chip cleaning of this example.
The test of sequence testing chip cleaning reagent properties is as follows：
1, volatilize time test on surface
The sequence testing chip cleaning reagent of 2 μ L is placed in sequence testing chip surface, its time volatilized completely is measured, is characterized with this
It volatilizees the time on the surface of sequence testing chip cleaning reagent.Test result is as shown in table 1.
As a comparison, the Illumina of the pure water of 2 μ L and 2 μ L recommendation cleaning reagent are equally placed in sequence testing chip table respectively
Face measures its time volatilized completely, characterizes the respective surface volatilization time with this.Test result is as shown in table 1.
2, light transmittance is tested
Using OU4220 it is visible/infrared/UV transmittance instrument carry out the test of sequence testing chip light transmittance, specifically, booting etc.
To 10min, by sequence testing chip be put into OU4220 it is visible/infrared/UV transmittance instrument measuring mouth tests.Detailed test method
With reference to OU4220 operation instruction.Particular technique parameter is as follows：
The scope of application：Glass, Coating Materials, organic material, coating；Measurement range：0-100%；Light source：Infrared light, can
Light-exposed, black light；Resolution ratio：0.1%；Repeatability：0.2%；The error of indication：2%；Operating voltage：One dry cell of section five
(AA) or rechargeable battery；Measurement area：13mm is minimum；Measure thickness：20mm is maximum；Environment temperature：0-40℃.
This example have detected first the light transmittance before the cleaning of 10 sequence testing chips be respectively 44.5%, 46.1%, 45.1%,
44.1%, 43.6%, 43.9%, 43.8%, 44.1%, 44.2%, 44%, average out to 44.34%.
Then each sequence testing chip cleaning reagent and pure water is respectively adopted and Illumina recommends cleaning reagent to sequencing core
Piece is cleaned, and the light transmittance of each sequence testing chip after detection cleaning, the results are shown in Table 1.
The cleaning way of sequence testing chip is：Cleaning reagent is dipped with kimtech dust-free paper, the dustless of cleaning reagent will be moistened with
Paper gently wipes sequence testing chip, and wiping is completed, and measures light transmittance after reagent to be cleaned is dry.
3, intensity A is tested
The light intensity of the constant Engineering Zone A base of chip, i.e. intensity A are the weights for reflecting the light transmission of sequence testing chip and reading
Want parameter.This example using BGISEQ-500 sequenator take pictures process acquisition the same area A base light intensity as sequence testing chip
Intensity A value.
This example have detected respectively using each sequence testing chip cleaning reagent and pure water and Illumina recommend cleaning reagent into
The intensity A value of sequence testing chip after row cleaning, the results are shown in Table 1.The cleaning way of sequence testing chip is the same as " 2, light transmittance
Test ".
1 sequence testing chip cleaning reagent of table and its performance test results
Table 1 the results show that lemon alcohol mass fraction 10%-25%, gleditsia sinensis extracting solution mass fraction 5%-14%, hydrogen-oxygen
Change potassium mass fraction 8%-20%, EDTA mass fraction 0.3%-2%, Quaity of Epoxidized Soybean Oil score 1%-7%, polyether-modified
Silicone oil mass fraction 0.1%-3%, ethyl alcohol mass fraction 10%-20%, the sequence testing chip of pure water mass fraction 23%-60% are clear
Reagent is washed, various aspects comprehensive performance is preferable；It is all optimal to be especially formulated 14 properties；Formula 14 in addition to surface volatilization the time compared with
Illumina recommends other than the long 1s of cleaning reagent, and 14 surfaces of the formula volatilization time is 3s, and light transmittance and intensity A value are all bright
It is aobvious to recommend cleaning reagent better than Illumina, also, surface volatilization time 3s can also receive.
For formula 29 with formula 30 since polyether modified silicon oil content is higher, the volatilization time is longer；Also, and it is formulated 30
Light transmittance it is relatively low.The light transmittance and intensity A value of formula 34 are preferable, but since epoxy soybean oil content is higher, table
The face volatilization time is longer.The lemon alcohol content of formula 35 is too low, be formulated 36 lemon alcohol content it is too high, therefore the light transmittance of the two
It is all obvious poor with intensity A value；The gleditsia sinensis extraction liquid hold-up of formula 37 is relatively low, so that intensity A value is relatively low；
The gleditsia sinensis extraction liquid hold-up of formula 38 is higher, so that light transmittance is slightly lower；As it can be seen that formula 29,30,34-38 is due to its part group
Divide not in the preferred scope of the application, so that sequence testing chip cleaning effect is all poor.In addition, pure water as a comparison,
The surface volatilization time is longer；Although Illumina recommends cleaning reagent, every detection effect is all preferable, there are toxicity greatly,
It is volatile, decompose the problems such as.
Embodiment two
This example is carried out using the sequence testing chip cleaning reagent (being hereinafter referred to as formulated 14) of the formula of test 14 in embodiment one
Test, meanwhile, cleaning reagent is recommended as a comparison using the identical Illumina of embodiment one, after more different reagent cleanings
Sequence testing chip, the influence to subsequent sequencing quality.Specifically, this example is tested using three libraries, three libraries are
The library phix control, by illumina kit article No.：FC-110-3001 is provided.Each library is equally divided into
Two parts, portion is sequenced using the sequence testing chip of the sequence testing chip cleaning reagent cleaning of formula 14, another use
Illumina recommends the sequence testing chip of cleaning reagent cleaning to be sequenced.Sequence testing chip cleaning method is the same as embodiment one.Except sequencing
Chip cleans other than the reagent difference used, other, including sequence testing chip, sequencing reagent, microarray dataset and method are all identical.
After the sequencing is completed, the Q30 value and FP% numerical value of machine data under each sequencing are counted.Wherein, Q30 is a germplasm of sequencing
Magnitude；Q30 mathematically for refer to, the probability of wrong identification is 0.1%, i.e., error rate 0.1% or accuracy are
99.9%；In sequencing evaluation, in order to assess the accuracy of lower machine reads sequencing, Q30 can be assessed and all base mass values are big
Ratio shared by Q30, this numerical value is higher, and reflection sequencing quality is better.FP% numerical value, that is, passfilter, refers to and meets mark
The percentage of quasi- cluster, it is associated with sequencing final data amount flux；This numerical value is higher, and reflection sequencing quality is better, obtains
Data volume is more.
Statistical result is as shown in table 2.
The Q30 value and FP% numerical value of each library of table 2 sequencing
| Test group | Q30 | FP% |
| Library one：Illumina recommends cleaning reagent | 74.47 | 83.9% |
| Library one：Formula 12 | 83.1 | 90.3% |
| Library two：Illumina recommends cleaning reagent | 70.6 | 84.5% |
| Library two：Formula 12 | 83.5 | 90.8% |
| Library three：Illumina recommends cleaning reagent | 86.6 | 86.9% |
| Library three：Formula 12 | 90.8 | 96.2% |
In table 2, " library one：Illumina recommends cleaning reagent " refer to that cleaning reagent is recommended using Illumina in library one
The sequence testing chip of cleaning is sequenced, " library one：Formula 12 " refers to library one using the sequence testing chip cleaning reagent of formula 12
The sequence testing chip of cleaning is sequenced, library two, library three and so on.
Table 2 the results show that in three libraries, using formula 12 the cleaning of sequence testing chip cleaning reagent sequence testing chip into
Row sequencing, Q30 value and FP% numerical value are all obviously due to the sequence testing chip using Illumina recommendation cleaning reagent cleaning；It says
It is bright, sequence testing chip can preferably be cleaned using the sequence testing chip cleaning reagent of formula 12, cleaning is more clean, thorough, thus
Better guarantee is provided for subsequent sequencing.
The foregoing is a further detailed description of the present application in conjunction with specific implementation manners, and it cannot be said that this Shen
Specific implementation please is only limited to these instructions.For those of ordinary skill in the art to which this application belongs, it is not taking off
Under the premise of from the application design, a number of simple deductions or replacements can also be made.
Claims (8)
1. a kind of reagent for sequence testing chip cleaning, it is characterised in that：Comprising lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide,
EDTA, epoxidized soybean oil, polyether modified silicon oil, ethyl alcohol and pure water.
2. reagent according to claim 1, it is characterised in that：By lemon alcohol, the 5%-14% of mass fraction 10%-25%
Gleditsia sinensis extracting solution, the potassium hydroxide of 8%-20%, 0.3%-2% EDTA, 1%-7% epoxidized soybean oil, 0.1%-3%
Polyether modified silicon oil, 10%-20% ethyl alcohol and 23%-60% pure water composition.
3. reagent according to claim 1, it is characterised in that：The mass fraction of the lemon alcohol is 20%, and gleditsia sinensis extracts
The mass fraction of liquid is 8%, and the mass fraction that the mass fraction of potassium hydroxide is 10%, EDTA is 2%, the matter of epoxidized soybean oil
Measuring score is 5%, and the mass fraction of polyether modified silicon oil is 3%, and the mass fraction of ethyl alcohol is 20%, and the mass fraction of pure water is
32%.
4. the preparation method of reagent according to claim 1-3, it is characterised in that：Including lemon is added into pure water
Lemon alcohol, gleditsia sinensis extracting solution, potassium hydroxide, EDTA, ethyl alcohol, it is closed to be stirred to react, it then adds epoxidized soybean oil and polyethers changes
Property silicone oil, stir evenly, that is, obtain for sequence testing chip cleaning reagent.
5. the preparation method according to claim 4, it is characterised in that：The closed time being stirred to react is 0.5h-1h.
6. preparation method according to claim 4 or 5, it is characterised in that：The mixing time stirred evenly is
10min-20min。
7. reagent according to claim 1-3 is in the environment-friendly cleaning agent that preparation is cleaned for glass or hardware
Using.
8. a kind of environment-friendly cleaning agent cleaned for glass or hardware, it is characterised in that：It include right in the environment-friendly cleaning agent
It is required that the described in any item reagents of 1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810542121.7A CN108913376B (en) | 2018-05-30 | 2018-05-30 | Reagent for cleaning sequencing chip and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810542121.7A CN108913376B (en) | 2018-05-30 | 2018-05-30 | Reagent for cleaning sequencing chip and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108913376A true CN108913376A (en) | 2018-11-30 |
| CN108913376B CN108913376B (en) | 2020-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810542121.7A Active CN108913376B (en) | 2018-05-30 | 2018-05-30 | Reagent for cleaning sequencing chip and preparation method and application thereof |
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| CN (1) | CN108913376B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112048401A (en) * | 2020-08-28 | 2020-12-08 | 上海符贝基因科技有限公司 | Micro-fluidic chip cleaning agent and method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566304A (en) * | 2003-06-13 | 2005-01-19 | 李小平 | Full automatic biochemical analyzer cleaning agent |
| CN101880608A (en) * | 2010-06-28 | 2010-11-10 | 深圳市成为生物科技有限公司 | Natural solid cleaning agent |
| CN102102051A (en) * | 2010-12-01 | 2011-06-22 | 宁波美康盛德生物科技有限公司 | Alkaline cleaning solution applied to automatic biochemical analyzer |
| CN103773631A (en) * | 2014-02-12 | 2014-05-07 | 深圳市职业病防治院 | Cleaning liquid for coagulometer |
| CN106381238A (en) * | 2016-08-30 | 2017-02-08 | 四川达文西科技有限公司 | Glass cleaning agent |
-
2018
- 2018-05-30 CN CN201810542121.7A patent/CN108913376B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566304A (en) * | 2003-06-13 | 2005-01-19 | 李小平 | Full automatic biochemical analyzer cleaning agent |
| CN101880608A (en) * | 2010-06-28 | 2010-11-10 | 深圳市成为生物科技有限公司 | Natural solid cleaning agent |
| CN102102051A (en) * | 2010-12-01 | 2011-06-22 | 宁波美康盛德生物科技有限公司 | Alkaline cleaning solution applied to automatic biochemical analyzer |
| CN103773631A (en) * | 2014-02-12 | 2014-05-07 | 深圳市职业病防治院 | Cleaning liquid for coagulometer |
| CN106381238A (en) * | 2016-08-30 | 2017-02-08 | 四川达文西科技有限公司 | Glass cleaning agent |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112048401A (en) * | 2020-08-28 | 2020-12-08 | 上海符贝基因科技有限公司 | Micro-fluidic chip cleaning agent and method thereof |
| CN112048401B (en) * | 2020-08-28 | 2022-05-17 | 上海符贝基因科技有限公司 | Micro-fluidic chip cleaning agent and method thereof |
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| CN108913376B (en) | 2020-11-24 |
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