CN102090335A - Culture medium used in rooting phase of plant tissue culture - Google Patents
Culture medium used in rooting phase of plant tissue culture Download PDFInfo
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- CN102090335A CN102090335A CN2010105804542A CN201010580454A CN102090335A CN 102090335 A CN102090335 A CN 102090335A CN 2010105804542 A CN2010105804542 A CN 2010105804542A CN 201010580454 A CN201010580454 A CN 201010580454A CN 102090335 A CN102090335 A CN 102090335A
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- plant tissue
- river sand
- rooting
- thin river
- medium used
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Abstract
The invention discloses a culture medium used in rooting phase of plant tissue culture, comprising cleaned thin river sand, mineral element, nutrient element and hormone, wherein the using amount thereof is 1/4 MS. The grain diameter of the thin river sand is 0.1 to 1 mm. The invention has the following advantages: the thin river sand with zero cost is adopted to replace the agar so that the rooting speed can be increased by 30%; the quality of the root system is very good, and the root system is similar to the rooting system under soil environment. The thin sand can be used repeatedly; the fungus pollution can be reduced by 60%; and the growth of the seedling is not influenced even if being polluted.
Description
Technical field
The present invention relates to agricultural technology field, specifically is a kind of Plant Tissue Breeding stage medium of taking root.
Background technology
The existing Plant Tissue Breeding stage medium of taking root mainly is agar+nutritive element+hormone.The nutrition flowability is poor in the agar, and gas permeability is poor, and bacterium breeds easily.Though liquid culture allows the flowability problem that has solved nutrition, but need to adopt special-purpose concussion shaking table satisfy the demand of root system to oxygen by concussion.And it is tender and crisp that agar medium is taken root, and the root hair is few.Entering the transplanting stage will shift to an earlier date hardening, and transplanting also needs to have shaded early stage, and survival rate is all below 80%.
Summary of the invention
In order to overcome above-mentioned defective, the invention provides a kind of Plant Tissue Breeding stage medium of taking root, replace agar with the thin river sand that cleans up, make the overall broth material cost reduce by 40%.
Technical scheme of the present invention is achieved in that the Plant Tissue Breeding stage medium of taking root, and contains the thin river sand, mineral element, nutritive element and the hormone that clean up, and its consumption is 1/4MS.
The particle diameter of described thin river sand is 0.1 ~ 1mm.
The invention has the beneficial effects as follows: adopt thin river sand zero cost to replace agar, rooting rate improves 30%, and the root system quality is fabulous, being similar to takes root following of soil environment is, fine sand can also be reused, and mushroom pollute to reduce by 60%, even and polluted and also do not influence a bottle seedling growth.
Embodiment
The Plant Tissue Breeding stage medium of taking root, containing the thin river sand particle diameter that cleans up is 0.1 ~ 1mm, mineral element, nutritive element and hormone, its consumption is 1/4MS.
Adopt thin river sand zero cost to replace agar, rooting rate improves 30%, and the root system quality is fabulous, and being similar to takes root following of soil environment is, fine sand can also be reused, and mushroom pollutes and reduces by 60%, even and polluted and also do not influence bottle seedling and grow.
The above; only be the preferable embodiment of the present invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, all should be encompassed within protection scope of the present invention.
Claims (2)
1. the Plant Tissue Breeding stage medium of taking root is characterized in that, the described Plant Tissue Breeding stage medium of taking root contains thin river sand, mineral element, nutritive element and the hormone that cleans up, and its consumption is 1/4MS.
2. the Plant Tissue Breeding according to claim 1 stage medium of taking root is characterized in that the particle diameter of described thin river sand is 0.1 ~ 1mm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2010105804542A CN102090335A (en) | 2010-12-09 | 2010-12-09 | Culture medium used in rooting phase of plant tissue culture |
Applications Claiming Priority (1)
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CN2010105804542A CN102090335A (en) | 2010-12-09 | 2010-12-09 | Culture medium used in rooting phase of plant tissue culture |
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CN102090335A true CN102090335A (en) | 2011-06-15 |
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CN2010105804542A Pending CN102090335A (en) | 2010-12-09 | 2010-12-09 | Culture medium used in rooting phase of plant tissue culture |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102550420A (en) * | 2012-01-16 | 2012-07-11 | 北京农学院 | Method for preparing Damascus rose tissue culture rooting medium |
CN102742508A (en) * | 2012-08-08 | 2012-10-24 | 昆明琪鼎权生物科技有限公司 | Method for utilizing tissue culture bag to simulate ecology to perform plant tissue culture |
CN108419678A (en) * | 2018-05-30 | 2018-08-21 | 楚雄师范学院 | A kind of chiltern plant tissue culture media and the preparation method and application thereof |
-
2010
- 2010-12-09 CN CN2010105804542A patent/CN102090335A/en active Pending
Non-Patent Citations (3)
Title |
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王淑勤等: "用河沙和蛭石制作植物组织培养的培养基", 《河北林业科技》 * |
赵建萍等: "培养因子对艾西丝南瓜芽增殖及不定根形成的影响", 《植物学通报》 * |
马纯艳等: "激素及支持物对丰花月季快速繁殖的影响", 《吉林林业科技》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102550420A (en) * | 2012-01-16 | 2012-07-11 | 北京农学院 | Method for preparing Damascus rose tissue culture rooting medium |
CN102550420B (en) * | 2012-01-16 | 2016-07-13 | 北京农学院 | The preparation method of Damascus rose tissue culture rooting medium |
CN102742508A (en) * | 2012-08-08 | 2012-10-24 | 昆明琪鼎权生物科技有限公司 | Method for utilizing tissue culture bag to simulate ecology to perform plant tissue culture |
CN108419678A (en) * | 2018-05-30 | 2018-08-21 | 楚雄师范学院 | A kind of chiltern plant tissue culture media and the preparation method and application thereof |
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Application publication date: 20110615 |