CN102008754B - Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration - Google Patents
Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration Download PDFInfo
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- CN102008754B CN102008754B CN201010586793.1A CN201010586793A CN102008754B CN 102008754 B CN102008754 B CN 102008754B CN 201010586793 A CN201010586793 A CN 201010586793A CN 102008754 B CN102008754 B CN 102008754B
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Abstract
The invention relates to a cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration. The cosmetic filler is prepared from the following components by weight percent: 1-20% of cell growth factor, 5-80% of cell growth nutrient and 0.1-20% of hyaluronic acid gel; and the preparation method of the cosmetic filler comprises: fully dissolving the cell growth factor and the cell growth nutrient in water; adding the hyaluronic acid gel powder, and fully stirring to enable the cell growth factor and the cell growth nutrient to be absorbed by crosslinked hyaluronic acid gel. In the invention, the hyaluronic acid gel and the growth factor are compounded for filling defected soft tissues, thus the purposes of slowly releasing the growth factor to promote cell growth and repairing the defected soft tissues are achieved, and the effectiveness is durable; and extensive trials show that the cosmetic filler can become effective as soon as the cosmetic filler is injected, the handfeel is soft and real, rejection is avoided, red and swollen phenomena are prevented in the injection process, the growth effect is favorable, and the defect of short retention time caused by only injecting hyaluronic acid gel is solved.
Description
Technical field
The present invention relates to a kind of cosmetic filler that promotes filler, the especially effect of human body soft tissue regeneration lasting, there is the promotion soft tissue regeneration of long-term efficacy.
background information
Tissue often affects face appearance etc. because the various pains of injury, disease, contingency, wound etc. cause the default or facial wound of soft tissue, need to fill and repair or beauty treatment etc., to repair default soft tissue or to make people's face well-done, generous or increase beautiful sense etc. by simple cosmetic surgery; In prior art, as the filler in the implant into body of reparation or cosmetic surgery, normally used is paraffin, silica gel, animal collagen, polyacrylamide gel etc., because of the material that is not human body self, easily there is rejection in these materials, some material even produces the stronger non-constant of rejection, histocompatibility with human body, cause a lot of side effect, its scope of application is subject to larger restriction.
In prior art, hyaluronic acid derivatives is a kind of good soft tissue filler, is the important component part of tissue, and histocompatibility is very good, as animal collagen, does not likely have rejection.But directly use single hyaluronic acid derivatives as soft tissue filling material, will decompose absorption because of metabolism after a while, do not reach the object of long-term filling.
In prior art, the cell growth factor of Promote cell's growth, there is the function that promotes histiocyte regeneration, but the reproduction speed of cell and cell are subject to the time length of somatomedin effect to have obvious relation, that is to say that cell only just can ceaselessly grow under the stimulation that is subject to for a long time cytokine, however direct injection cell growth factor, suitable of short duration of time of stopping at body local, what have only has 1-2 hour, does not reach the object of stimulating cellular growth, and its effect is difficult to lastingly.
goal of the invention
The present invention is directed in prior art as repair or the implant into body body of cosmetic surgery in the existing above-mentioned deficiency of filler, a kind of cosmetic filler with the promotion soft tissue regeneration of long-term efficacy is provided.
Technical scheme of the present invention: there is the cosmetic filler of the promotion soft tissue regeneration of long-term efficacy, it is characterized in that comprising the component of following percentage by weight,
The cell growth factor of Promote cell's growth, content is 0.1-20%;
Nutrient for cell growth, content is 5-80%;
Hyaluronic acid derivatives, content is 0.1-20%;
Above-mentioned cell growth factor, nutrient for cell growth are pressed to aforesaid part by weight, fully be dissolved in the water, then add hyaluronic acid derivatives powder, fully stir, make cell growth factor and nutrient for cell growth in solution be crosslinked the absorption of hyaluronic acid derivatives powder.
Further feature is: also comprise human body autologous tissue stem cell, addition accounts for the 1-20% of gross weight.
After adding hyaluronic acid derivatives fully to stir, add catalyst, the factor of Growth of Cells and hyaluronic acid are reacted, somatomedin joint is propped up to hyaluronic acid.
The present invention has the cosmetic filler of the promotion soft tissue regeneration of long-term efficacy, is equivalent to prior art, and tool has the following advantages:
1, histocompatibility is very good, substantially rejection can not occur; The present invention is grouped together hyaluronic acid derivatives and cell growth factor and the needed nutrient of Growth of Cells, and the histocompatibility of hyaluronic acid derivatives and human body is very good, rejection can not occur.
2, effect persistency is strong, has long-term effect; Hyaluronic acid derivatives plays interim filling and supports
Effect, somatomedin and nutrient for cell growth are had to slow releasing function simultaneously, make hyaluronic acid derivatives border cell be subject to for a long time the effect of cell growth factor and grow, when hyaluronic acid is when slowly metabolism absorbs in vivo, somatomedin and nutrient for cell growth be slow release also, has just promoted border cell's growth, so how many cells of cross-linked-hyaluronic acid metabolism just growth are how many, just in time reach balance, so just reach the default object of long-term repair soft tissue, just there is long-term efficacy.
3, in addition, the present invention can also by prior cultured human body autologous tissue stem cell as fibroblast, fat before cell etc. put as accelerate tissue growth in this filler, these cells have merisis ability, they can utilize in gel and surrounding tissue nutritional labeling, and under the effect of somatomedin, growth is rapider.
4, filler of the present invention, a kind of promotion soft tissue regeneration, the comparatively desirable filler of effect that can be transplanted in human body, be widely used in filling skin depressions, tissue defect, also can hold as wrinkle removing, rich, augmentation rhinoplasty, chin lengthening, breast enlargement etc. for physical beauty.
5, through extensively on probation, injection takes effect immediately, soft true, not only there is no rejection, also there is no red and swollen phenomenon, and growth result is good during injection, has overcome simple short defect of Injection of Hyaluronic Acid gel retention time.
Summary of the invention
The cosmetic filler with the promotion soft tissue regeneration of long-term efficacy, is characterized in that comprising the component of following percentage by weight,
The cell growth factor of Promote cell's growth, content is 0.1-20%;
Nutrient for cell growth, content is 5-80%;
Hyaluronic acid derivatives, content is 0.1-20%;
The cell growth factor of described Promote cell's growth, mainly refer to fibroblast growth factor, epidermal growth factor, platelet class somatomedin, insulin like growth factor, transforming growth factor, nerve growth factor, calf blood protein-removed extraction etc., include but not limited to above-mentioned concrete cell growth factor; Can be alone wherein a kind of, also can be by the cell growth factor of two or more multiple proportioning being obtained by mixing with arbitrary proportion wherein; According to the different position of human body and different treatment, beauty treatment needs, conventionally select suitable a kind of cell growth factor for best, the mixed type cell growth factor that also can adopt various kinds of cell somatomedin to be obtained by mixing with arbitrary proportion.
Nutrient for cell growth, mainly refers to vitamin, trace element, electrolyte, aminoacid, glucose, organic acid etc., includes but not limited to above-mentioned concrete nutrient or nutrition goods and materials; Nutrient for cell growth, as a kind of material, has had the product of plurality of specifications to sell on market, can be classified as prior art, adopts the proportioning of prior art to be obtained by mixing.
Hyaluronic acid derivatives, as slow releasing agent, plays slow release, filling, support.
Explained hereafter method of the present invention is: the cell growth factor of above-mentioned Promote cell's growth, nutrient for cell growth are pressed to aforesaid part by weight, fully be dissolved in the water, need if desired stirring or rising temperature etc., make it to reach the requirement of Promote cell's growth, then add cross-linked hyaluronic acid gel powder, fully stir, make cell growth factor and nutrient for cell growth in solution be crosslinked the absorption of hyaluronic acid derivatives powder completely, and can not separate out moisture.
Also comprise human body autologous tissue stem cell, by prior cultured human body autologous tissue stem cell, as cell or other stem cell before fibroblast, fat, add in this filler, as supplementary element, play the effect of accelerate tissue growth.The addition of human body autologous tissue stem cell accounts for the 1-20% of gross weight.
Can add hyaluronic acid derivatives solution fully to stir, and after mix homogeneously, adding weight is that 0.005-1% aldehyde material is as catalyst, what this aldehyde material was conventional is formaldehyde, Biformyl, glutaraldehyde etc., but be not limited to these three kinds, the factor and the hyaluronic acid derivatives of Growth of Cells are reacted, somatomedin joint is propped up to hyaluronic acid, with the cross-linking agent that weight is 0.1-20%, make cross-linking hyaluronic acid form gel again, what cross-linking agent was conventional is divinylsulfone, also can use hydrazides, 1, 4 butanediol glycidyl ethers, carbodiimide etc., but it is several to be not limited to this.
Cell growth factor of the present invention, its content can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc.; Nutrient for cell growth of the present invention, its content can be 5%, 6%, 8%, 10%, 15%, 20%, 28%, 30%, 35%, 40%, 45%, 48%, 50%, 54%, 58%, 60%, 65%, 68%, 70%, 75%, 78%, 80% etc.; Hyaluronic acid derivatives of the present invention, its content can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc.; Human body of the present invention autologous tissue stem cell, its content can be 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc.; Cross-linking agent of the present invention, its content can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc., can meet needs of the present invention.
Embodiment 1: by cell growth factor and nutrient for cell growth by a certain percentage of the weight, cell growth factor is 40 grams, nutrient for cell growth 1 standard bag (DMEM dehydrated medium is used for preparing 1000 ml vols), fully be dissolved in 1000 ml waters and (meet medical distilled water or pure water etc.), need if desired stirring or rising temperature etc., then add 20 grams of hyaluronic acid derivatives powder, fully stir and soak, make cell growth factor and nutrient for cell growth in solution be crosslinked hyaluronic acid derivatives absorption completely, no longer separate out after moisture, make filler of the present invention, under 100 degree, sterilize 30 minutes.
Embodiment 2: by cell growth factor and nutrient for cell growth by a certain percentage of the weight, cell growth factor is 45 grams, nutrient for cell growth 1 standard bag (DMEM dehydrated medium is used for preparing 1000 ml vols), fully be dissolved in 1000 ml waters and (meet medical distilled water or pure water etc.), need if desired stirring or rising temperature etc., make it to reach the requirement of Promote cell's growth, then add 25 grams of hyaluronic acids, after fully mixing, add 0.005% formaldehyde, the factor and the hyaluronic acid of Growth of Cells are reacted, cell growth factor joint is propped up to hyaluronic acid, then add 50 divinylsulfones to form gel, under 100 degree, sterilize 30 minutes.
Embodiment 3:, the filler making will be produced according to the inventive method, under aseptic condition, implant the successful human body of cultivation autologous tissue stem cell in advance, as (including but not limited to these cells) such as cells before fibroblast, fat, then the filler of having implanted human body autologous tissue stem cell is expelled to the place that human body needs.
Using method: the filler of the present invention of making is put into syringe, and the syringe needle with different length, injects the default position of soft tissue as required, makes local form reach desirable degree; Be expelled to the default position of human body soft tissue, play conduct or the beautifying face effect of repairing.
Claims (2)
1. the cosmetic filler with the promotion soft tissue regeneration of long-term efficacy, is characterized in that comprising the component of following percentage by weight,
The cell growth factor of Promote cell's growth, content is 0.1-20%;
Nutrient for cell growth, content is 60-80%;
Hyaluronic acid derivatives, content is 0.1-20%;
Described cell growth factor, comprises fibroblast growth factor, epidermal growth factor, and insulin like growth factor, alone wherein a kind of, or be obtained by mixing with arbitrary proportion with wherein two or more;
Above-mentioned cell growth factor, nutrient for cell growth are pressed to aforesaid part by weight, fully be dissolved in the water, then add hyaluronic acid derivatives powder, fully stir, make cell growth factor and nutrient for cell growth in solution be crosslinked the absorption of hyaluronic acid derivatives powder;
After adding hyaluronic acid derivatives fully to stir, add weight be 0.005-1% aldehyde material as catalyst, the factor of Growth of Cells and hyaluronic acid derivatives are reacted, somatomedin is grafted on hyaluronic acid; With the cross-linking agent that weight is 0.1-20%, make cross-linking hyaluronic acid form gel again, cross-linking agent is divinylsulfone, or hydrazides, or 1,4 butanediol glycidyl ethers, or carbodiimide.
2. the cosmetic filler according to claim 1 with the promotion soft tissue regeneration of long-term efficacy, is characterized in that: also comprise human body autologous tissue stem cell, addition accounts for the 1-20% of gross weight.
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| CN201010586793.1A CN102008754B (en) | 2010-12-14 | 2010-12-14 | Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration |
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| CN102008754B true CN102008754B (en) | 2014-01-15 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805880A (en) * | 2011-05-31 | 2012-12-05 | 上海国睿生命科技有限公司 | Tissue engineering gel scaffold material, preparation method and use thereof |
| CN102813961A (en) * | 2011-06-10 | 2012-12-12 | 冯淑芹 | Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof |
| CN104014000A (en) * | 2014-06-10 | 2014-09-03 | 白晋 | Injection reagent for cosmetic filling |
| CN105030647B (en) * | 2015-09-14 | 2018-04-24 | 广州赛莱拉干细胞科技股份有限公司 | A kind of preparation for reducing wrinkle and preparation method thereof |
| CN105106093B (en) * | 2015-09-14 | 2018-06-15 | 广州赛莱拉干细胞科技股份有限公司 | A kind of face rejuvenation cosmetic formulation and preparation method thereof |
| CN105233270A (en) * | 2015-11-12 | 2016-01-13 | 上海昊海生物科技股份有限公司 | Medicine composition for water-optical treatment and application of medicine composition |
| CN106421920B (en) * | 2016-11-16 | 2019-05-14 | 沈阳细胞治疗工程技术研发中心有限公司 | A kind of fat filler and preparation method thereof |
| CN112386519A (en) * | 2019-08-16 | 2021-02-23 | 上海可米日化股份有限公司 | Beauty gel and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785852A (en) * | 2010-01-21 | 2010-07-28 | 孙杰 | Dermis trace intervention anti-wrinkle cosmetic preparation and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101785852A (en) * | 2010-01-21 | 2010-07-28 | 孙杰 | Dermis trace intervention anti-wrinkle cosmetic preparation and method |
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