CN108465124A - A kind of self-crosslinking injection beauty packing material and preparation method - Google Patents
A kind of self-crosslinking injection beauty packing material and preparation method Download PDFInfo
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- CN108465124A CN108465124A CN201810348070.4A CN201810348070A CN108465124A CN 108465124 A CN108465124 A CN 108465124A CN 201810348070 A CN201810348070 A CN 201810348070A CN 108465124 A CN108465124 A CN 108465124A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
- A61L2300/622—Microcapsules
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
Abstract
The present invention relates to a kind of self-crosslinking injection beauty packing material and preparation methods, belong to injection beauty treatment material technical field.The technical problem to be solved by the present invention is to provide a kind of self-crosslinking injection beauty packing material and preparation methods.The self-crosslinking injects beauty packing material, is made of A agent and B agent, and A agent and B agent are independently stored, wherein A agent is calcium citrate microcapsules；B agent is by sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline composition, its component is simple, cost is relatively low, it mixes when in use, sodium alginate is mixed with the calcium citrate sustained-release micro-spheres with slow-release function, the calcium ion sustained-release in body, calcium ion makes sodium alginate self-crosslinking, to network readezvous point matter acid, alginic acid glue and hyaluronic acid is set to lose rheological characteristic, not only it is effectively prevented the degradation of hyaluronic acid, and stablize in body filling plasticity, moulding effect is good, with good biocompatibility, overcome the defect that bio-toxicity is easily caused using cross-linked-hyaluronic acid.
Description
Technical field
The present invention relates to a kind of self-crosslinking injection beauty packing material and preparation methods, belong to injection beauty treatment material technology neck
Domain.
Background technology
Today's society, shaping have been not only the patent in injury repair field, more become people and pursue beautiful means.
With the improvement of living standards, the development of shaping technique obtains this beautiful approach by shaping and has gradually been connect by people
By and trust.The crowd for receiving shaping has also been spread via the amusement circles star for being engaged in performing career to ruck, from shaping
The great demand market that beauty treatment material is possessed, we can predict its good development prospect.
Spring bamboo after various shaping and beauty means such as rain continues to bring out, including traditional plastic surgery operations, microsurgery
Technology, Skin expansion and injection transplantation technology etc., wherein injection beauty is particularly subject to pursuing for shaping and beauty hospitalier,
The extensive approval that medical and beauty treatment industry professional and people seeking beauty are obtained in worldwide, becomes the following medical and beauty treatment
Developing direction.
It is that filler is injected body local to inject beauty, to reach the beauty method for correcting skin defect.Injection is beautiful
Appearance is one kind of non-operating shaping beauty, is injected biomaterial or artificial synthesized biocompatible material using the method for injection
Enter skin corium or subcutaneously reaches the method for the major class plastic operation for reducing skinfold or plastotype by the different mechanism of action.
Have the characteristics that safe, painless, efficient.
The key for injecting beauty is injection material, has collagen, hyaluronic acid etc. at present and is used for injection material.But by
In being difficult to form support, within some months, after being absorbed by organisms, build repair disappears.Most commonly used hyaluronic acid
It is the important component of humans and animals skin, vitreum, joint lubrication liquid and cartilaginous tissue, it is by (1- β -4) D- glucuronic acids
(1- β -3) N- acetyl group-D- aminoglucose dissacharide units repeat to be formed by connecting, and are widely used in prosthesis, eye hand
Art fills wrinkle as beauty product.Due to being degraded rapidly by the enzymolysis of hyaluronidase in vivo, it should
Class material needs are injected for a long time.In order to promote retention time, mainly dropped both at home and abroad by cross-linked-hyaluronic acid at present
Low degradation problem.However since crosslinking agent has very high bio-toxicity, potential danger is caused to injection beauty.
In consideration of it, constantly exploring the pass that persistence is good, good biocompatibility injection material is beauty and shaping sound development
Key.
Application No. is 200810009192.7 Chinese invention patents to disclose a kind of injection polymethyl methacrylate
With crosslinking hyaluronic acid mixed gel and preparation method thereof, the polymethyl methacrylate and crosslinking hyaluronic acid mixed gel are
It is made of poly (methyl methacrylate) micro-sphere, cross-linked hyaluronic acid gel particle and hyaluronic acid or its salting liquid, wherein poly- first
Ranging from account for 0.5%~30% shared by base methyl acrylate microsphere volume, cross-linked hyaluronic acid gel particle and hyaluronic acid or
The volume ratio range of its salting liquid is 1: 19~19: 1.The gel is the injection for beauty and shaping, is had efficiently, lasting
Moulding feature.But the injection material is poor to the hyaluronic acid property held, and biocompatibility is poor, easily causes local red and swollen, pain
Bitterly, bleeding, and polymethyl methacrylate is disperseed and is injected with microspheres form, is easy to form agglomerate, is easily flowed in skin,
Fixed filling effect is poor.
Application No. is 201710575786.3 Chinese invention patents to disclose a kind of injection crosslinking polyglutamic acid gel
Polyglutamic acid aqueous solution is added in the oil phase containing emulsifier by microsphere suspension and preparation method thereof, passes through emulsification shape
At polyglutamic acid gel micro-ball, then crosslinking agent is added, heating stirring crosslinking, liquid layer under centrifuging and taking, is carried out with detergent after crosslinking
Washing, the polyglutamic acid microballoon after washing is suspended in physiological saline or physiological buffer and is swollen, and obtains crosslinking polyglutamic acid
Gel micro-ball suspension.This method is easy to operate, is easy to industrialized production.Injection prepared by this method is crosslinked polyglutamic acid cure
Application of the glue microballoon in injecting beauty, it is possible to reduce inject resistance when injection, reduce the risk of injection operation error.So
And the material still uses crosslinking Treatment, can overcome the defect of degradation in vivo.Since crosslinking agent has very high life
Object toxicity will give injection beauty to cause potential danger.
To sum up, the study found that it is the hyaluronic acid of injection that injection material for beauty and shaping the reason of needing continuous injection
Be easy degradation, and inject non-degradable material then biocompatibility is poor, in order to delay the degradation of hyaluronic acid, the prior art be into
Row crosslinking Treatment, and crosslinking agent can then become biotoxin, cause side effect.
Therefore, it is necessary to a kind of other materials for injecting beauty, not only have good biocompatibility, and can
More lasting presence, this has particularly important meaning for injection beauty.
Invention content
To overcome drawbacks described above, the present invention to propose that a kind of self-crosslinking injects beauty packing material and preparation method.
First technical problem that the present invention solves is to provide a kind of self-crosslinking injection beauty packing material.
Self-crosslinking of the present invention injects beauty packing material, is made of A agent and B agent, and A agent and B agent are independently stored, wherein A
Agent is calcium citrate microcapsules；B agent is grouped as by the group of following parts by weight：10~20 parts of sodium alginate, hyaluronic acid 5~30
Part, 10~25 parts of collagen, 1~15 part of vitamin C, 80~120 parts of physiological saline.
Self-crosslinking of the present invention injects beauty packing material, and A agent and B agent are independently stored, and is mixed when injection uses, A agent,
The mass ratio of B agent mixing is 1:15.Water-soluble sodium alginate is micro- with the calcium citrate sustained release with slow-release function in injection
Ball mixes, and the calcium ion sustained-release in body, calcium ion makes sodium alginate self-crosslinking, to network readezvous point matter acid, makes alginic acid
Glue and hyaluronic acid lose rheological characteristic, are not only effectively prevented the degradation of hyaluronic acid, and stablize in body filling plasticity, modeling
Shape effect is good, has good biocompatibility, overcomes the defect for easily causing bio-toxicity using cross-linked-hyaluronic acid.This is filled out
Filling material and especially being repaired to Facial Depression filling has obvious action.
A agent is mainly calcium citrate microcapsules.Microcapsules refer to a kind of miniature vessel with polymeric shell wall or packet object.Tool
Refer to by a certain purpose thing for body（Core or interior phase）With various natural or synthesis high-molecular compound continuous films（Wall or
Foreign minister）Cladding is got up completely, and at all lossless to original chemical property of purpose thing, then increasingly by certain outside stimulus
Or slow releasing function makes the function of purpose thing be showed again in outside, or play protection core material by the shielding action of cyst wall
Effect.In the present invention, purpose thing is calcium citrate, and cyst wall is chitosan.Calcium citrate microcapsules i.e. of the invention be with
Calcium citrate is core, using chitosan as the microcapsules of wall.In use, cyst wall is degraded, and slowly releases internal lemon
Sour calcium is catalyzed sodium alginate self-crosslinking.
Preferably, the grain size of the calcium citrate microcapsules is 5~20 microns.
B agent is the mixture of various raw materials.By sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline
Mixing, specific component and proportioning can constitute the self-crosslinking injection beauty packing material of the present invention.
Wherein, sodium alginate is the by-product after extracting iodine and mannitol in the kelp or sargassum of brown algae,
Molecule is by beta-D-mannuronic acid（β-D-mannuronic, M）With α-L- guluronic acids（α-L-guluronic, G）It presses（1→
4）It is keyed, is a kind of natural polysaccharide, with stability, dissolubility, viscosity and the safety needed for pharmaceutical preparation auxiliary material.
The present invention uses the sodium alginate of specific part, can be crosslinked under the action of calcium ion, better network readezvous point matter acid, together
When so that alginic acid glue and hyaluronic acid is lost rheological characteristic, be not only effectively prevented the degradation of hyaluronic acid, and fill in body
Plasticity is stablized, and moulding effect is good.
The dissacharide units sodium hyaluronate of hyaluronic acid, i.e. D-Glucose aldehydic acid and N-acetyl-glucosamine composition
（Hyaluronan）, also known as uronic acid, basic structure are by two dissacharide units D-Glucose aldehydic acid and N-acetyl-glucosamine group
At large-scale polysaccharide.It is different from other mucopolysaccharides, its not sulfur-bearing.Hyaluronic acid is with its unique molecular structure and physicochemical property
Show that a variety of important physiological functions, such as lubricating joint adjust the permeability of vascular wall, regulatory protein matter, water in body
Electrolyte diffusion and operating promote wound healing etc..Particular, it is important that hyaluronic acid has special water retention, it
Hyalomitome molecule can carry 500 times or more of moisture, be the substance that moisture retention is best in presently found nature, be referred to as managing
The natural moisturizing factor thought.The present invention uses main component of the hyaluronic acid as injection material.
Collagen is boiomacromolecule, the main component in animal connective tissue, and content is most in the mammalian body
Functional protein more, distribution is most wide, accounts for the 25%~30% of total protein.Collagen is extracted by Animal Skin, containing a large amount of
Polar group is moisturizing factor, and plays the role of that the tyrosine in skin is prevented to be converted into melanin, therefore collagen has pure day
The effects that right moisturizing, whitening, wrinkle resistant, nti-freckle, and the chemical composition of collagen, structure impart it be beauty basis.Glue
Former albumen is similar to the structure of human skin collagen, contains glycoprotein for non-soluble fiber shape, a large amount of amino are rich in molecule
Acid and hydrophilic group have certain surface-active and good compatibility, simultaneously because contain a large amount of hydroxyl in its molecule, because
This it have fairly good moisture-keeping function.And the structure of vitamin C is similar to glucose, is a kind of polyol, molecule
In the 2nd and the 3rd upper two adjacent enolic hydroxyls easily dissociate and disengage H+, it is also known as anti-bad therefore with the property of acid
Hematic acid.Therefore, there is the collagen and vitamin c centainly matched in self-crosslinking of the invention injection beauty packing material.
Physiological saline is 0.9% sodium-chloride water solution, and the present invention uses physiological saline to be used as solvent.
Each component complements each other in B agent, synergistic, and the proportioning of each component is crucial.Preferably, the B agent is by following
The group of parts by weight is grouped as；12~18 parts of sodium alginate, 10~20 parts of hyaluronic acid, 15~20 parts of collagen, vitamin C 5
~12 parts, 90~110 parts of physiological saline.It is furthermore preferred that the B agent is grouped as by the group of following parts by weight；16 parts of sodium alginate,
15 parts of hyaluronic acid, 18 parts of collagen, 8 parts of vitamin C, 100 parts of physiological saline.
Second technology that the present invention solves is that topic is to provide a kind of preparation method of self-crosslinking injection beauty packing material.
The preparation method of self-crosslinking injection beauty packing material of the present invention, includes the following steps：
A, A agent is prepared：By calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, emulsifier with mass ratio 3:0.1:
10：0.1 under 5000~8000rpm stirrings 10~15min obtain chitosan solution, it is then mixed with the calcium citrate of miniaturization
Even spraying drying is closed, calcium citrate microcapsules are obtained；That is A agent；
B, B agent is prepared：Sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline are mixed, stirs and evenly mixs, obtains
To B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature sterilizing, obtain self-crosslinking injection beauty packing material.
Present invention preferably employs spray drying processes microcapsules are prepared.Calcium citrate is scattered in chitosan solution
In, and emulsifier is added, it is prepared into emulsion, is then spray-dried, small liquid is atomized by fog-spray nozzle, these
When droplet is with hot air, the heated rapid evaporation of moisture of wall material is dissolved, so that wall material chitosan is solidified, is formed a kind of netted
Structure, calcium citrate are then trapped in " net ", form calcium citrate microcapsules.
Calcium citrate is particle not soluble in water, and Acidic Liquid, the dosage of acetic acid are dissolved in order to avoid calcium citrate is premature
It needs control in low concentration, and when being spray-dried, residence time mistake is not easy after the calcium citrate dispersion of miniaturization
It is long, preferably complete to be spray-dried in 3-5min.
Common emulsifier is suitable for the present invention, such as fatty acid mono glycidol, lauric monoglyceride, D-sorbite
Fat etc..
Preferably, the weight of the calcium citrate and the volume ratio of chitosan solution are 1~3g:10mL.
B step is the step of preparing B agent, mainly by sodium alginate, hyaluronic acid, collagen, vitamin C and physiology
Brine mixes, and stirs evenly.In order to ensure the stabilization of each component performance, it is preferred that the temperature of the stirring is not higher than 20
℃。
The a steps and b step of the present invention does not have time sequencing.
Step c carries out after a steps and b step are completed, and is packaging sterilizing step.Common room temperature sterilizing methods are suitable
For the present invention, it is preferred that the sterilizing is radiation sterilization.
Compared with prior art, the present invention has the advantages that：
The self-crosslinking of the present invention injects beauty packing material, is made of A agent and B agent, component is simple, and cost is relatively low, is using
When mix, water-soluble sodium alginate mixes with the calcium citrate sustained-release micro-spheres with slow-release function, calcium is sustained in body
Ion, calcium ion make sodium alginate self-crosslinking, to network readezvous point matter acid, alginic acid glue and hyaluronic acid are made to lose rheological characteristic,
It is not only effectively prevented the degradation of hyaluronic acid, and is stablized in body filling plasticity, moulding effect is good, has good biology
Compatibility overcomes the defect that bio-toxicity is easily caused using cross-linked-hyaluronic acid.The packing material especially fills out Facial Depression
Filling reparation has obvious action.
Specific implementation mode
In the following, the present invention will be further described in detail by way of specific embodiments, but this should not be interpreted as to the present invention
Range be only limitted to example below.Without departing from the idea of the above method of the present invention, according to ordinary skill
The various replacements or change that knowledge and customary means are made, should be included in the scope of the present invention.
Embodiment 1
A kind of self-crosslinking injection beauty packing material, is made of, and A agent and B agent are independently stored A agent and B agent, mixed when injection uses
Conjunction, the mass ratio that A agent, B agent mix is 1:15, wherein A agent is calcium citrate microcapsules；B agent by following parts by weight group
It is grouped as：10 parts of sodium alginate, 5 parts of hyaluronic acid, 10 parts of collagen, 15 parts of vitamin C, 80 parts of physiological saline, the lemon
Lemon acid calcium Microcapsules Size is 5~20 microns.
The preparation method of the packing material is as follows：
A, by calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, fatty acid mono glycidol with mass ratio 3:0.1:
10：0.1 5000rpm stirring under 10min obtain chitosan solution, be then uniformly mixed spraying with the calcium citrate of miniaturization
It is dry, obtain calcium citrate microcapsules, i.e. A agent；The weight of calcium citrate and the volume ratio of chitosan solution are 1g:10mL.
B, B agent is prepared：Sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline are mixed, are not higher than in temperature
It is stirred and evenly mixed under conditions of 20 DEG C, obtains B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature radiation sterilization, obtain self-crosslinking injection beauty packing material.
Embodiment 2
A kind of self-crosslinking injection beauty packing material, is made of, and A agent and B agent are independently stored A agent and B agent, mixed when injection uses
Conjunction, the mass ratio that A agent, B agent mix is 1:15, wherein A agent is calcium citrate microcapsules；B agent by following parts by weight group
It is grouped as：20 parts of sodium alginate, 30 parts of hyaluronic acid, 25 parts of collagen, 1 part of vitamin C, 120 parts of physiological saline, it is described
Calcium citrate microcapsules are using calcium citrate as core, and using chitosan as wall, grain size is 5~20 microns.The preparation of the packing material
Method is as follows：
A, by calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, lauric monoglyceride with mass ratio 3:0.1:
10：0.1 6000rpm stirring under 15min obtain chitosan solution, be then uniformly mixed spraying with the calcium citrate of miniaturization
It is dry, obtain calcium citrate microcapsules, i.e. A agent；The weight of calcium citrate and the volume ratio of chitosan solution are 3g:10mL.
B, B agent is prepared：By sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline mix, temperature not
It is stirred and evenly mixed under conditions of higher than 20 DEG C, obtains B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature radiation sterilization, obtain self-crosslinking injection beauty packing material.
Embodiment 3
A kind of self-crosslinking injection beauty packing material, is made of, and A agent and B agent are independently stored A agent and B agent, mixed when injection uses
Conjunction, the mass ratio that A agent, B agent mix is 1:15, wherein A agent is calcium citrate microcapsules；B agent by following parts by weight group
It is grouped as：12 parts of sodium alginate, 10 parts of hyaluronic acid, 20 parts of collagen, 5 parts of vitamin C, 90 parts of physiological saline, the lemon
Lemon acid calcium microcapsules are using calcium citrate as core, and using chitosan as wall, grain size is 5~20 microns.The preparation side of the packing material
Method is as follows：
A, by calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, sorbose alcohol ester with mass ratio 3:0.1:10：
0.1 8000rpm stirring under 10min obtain chitosan solution, be then uniformly mixed spray drying with the calcium citrate of miniaturization,
Obtain calcium citrate microcapsules, i.e. A agent；The weight of calcium citrate and the volume ratio of chitosan solution are 2g:10mL.
B, B agent is prepared：By sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline mix, temperature not
It is stirred and evenly mixed under conditions of higher than 20 DEG C, obtains B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature radiation sterilization, obtain self-crosslinking injection beauty packing material.
Embodiment 4
A kind of self-crosslinking injection beauty packing material, is made of, and A agent and B agent are independently stored A agent and B agent, mixed when injection uses
Conjunction, the mass ratio that A agent, B agent mix is 1:15, wherein A agent is calcium citrate microcapsules；B agent by following parts by weight group
It is grouped as：18 parts of sodium alginate, 20 parts of hyaluronic acid, 15 parts of collagen, 12 parts of vitamin C, 110 parts of physiological saline, it is described
Calcium citrate microcapsules are using calcium citrate as core, and using chitosan as wall, grain size is 5~20 microns.The preparation of the packing material
Method is as follows：
A, by calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, emulsifier with mass ratio 3:0.1:10：0.1
The lower 10min of 5000rpm stirrings obtains chitosan solution, and spray drying is then uniformly mixed with the calcium citrate of miniaturization, is obtained
Calcium citrate microcapsules, i.e. A agent；The weight of calcium citrate and the volume ratio of chitosan solution are 2g:10mL,.
B, B agent is prepared：By sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline mix, temperature not
It is stirred and evenly mixed under conditions of higher than 20 DEG C, obtains B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature radiation sterilization, obtain self-crosslinking injection beauty packing material.
Embodiment 5
A kind of self-crosslinking injection beauty packing material, is made of, and A agent and B agent are independently stored A agent and B agent, mixed when injection uses
Conjunction, the mass ratio that A agent, B agent mix is 1:15, wherein A agent is calcium citrate microcapsules；B agent by following parts by weight group
It is grouped as：15 parts of sodium alginate, 14 parts of hyaluronic acid, 17 parts of collagen, 10 parts of vitamin C, 100 parts of physiological saline, it is described
Calcium citrate microcapsules are using calcium citrate as core, and using chitosan as wall, grain size is 5~20 microns.The preparation of the packing material
Method is as follows：
A, by calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, sorbose alcohol ester with mass ratio 3:0.1:10：
0.1 8000rpm stirring under 10min obtain chitosan solution, be then uniformly mixed with the calcium citrate of miniaturization spraying do
It is dry, obtain calcium citrate microcapsules, i.e. A agent；The weight of calcium citrate and the volume ratio of chitosan solution are 2g:10mL.
B, B agent is prepared：By sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline mix, temperature not
It is stirred and evenly mixed under conditions of higher than 20 DEG C, obtains B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature radiation sterilization, obtain self-crosslinking injection beauty packing material.
Comparative example 1
A kind of self-crosslinking injects beauty packing material, is made of A agent and B agent, will A agent and B agent be blended after store, A agent, B agent are mixed
The mass ratio of conjunction is 1:15, wherein A agent is calcium citrate microcapsules；B agent is grouped as by the group of following parts by weight：Sodium alginate
15 parts, 14 parts of hyaluronic acid, 17 parts of collagen, 10 parts of vitamin C, 100 parts of physiological saline, the calcium citrate microcapsules
It is using calcium citrate as core, using chitosan as wall, grain size is 5~20 microns.The preparation method of the packing material is as follows：
A, by calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, sorbose alcohol ester with mass ratio 3:0.1:10：
0.1 8000rpm stirring under 10min obtain chitosan solution, be then uniformly mixed with the calcium citrate of miniaturization spraying do
It is dry, obtain calcium citrate microcapsules, i.e. A agent；The weight of calcium citrate and the volume ratio of chitosan solution are 2g:10mL.
B, B agent is prepared：By sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline mix, temperature not
It is stirred and evenly mixed under conditions of higher than 20 DEG C, obtains B agent；
C, packaging sterilizing：By A agent and B agent mixed storages, after room temperature radiation sterilization, self-crosslinking injection beauty packing material is obtained.
Comparative example 1 does not store A, B agent independently compared to embodiment 5, analyzes it and is crosslinked situation.
Comparative example 2
A kind of self-crosslinking injection beauty packing material, is made of, and A agent and B agent are independently stored A agent and B agent, mixed when injection uses
Conjunction, the mass ratio that A agent, B agent mix is 1:75, wherein A agent is calcium citrate microparticles；B agent by following parts by weight component
Composition：15 parts of sodium alginate, 14 parts of hyaluronic acid, 17 parts of collagen, 10 parts of vitamin C, 100 parts of physiological saline.The filling
The preparation method of material is as follows：
A, by calcium citrate miniaturization to micron order, i.e. A agent；
B, B agent is prepared：Sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline are mixed, are not higher than in temperature
It is stirred and evenly mixed under conditions of 20 DEG C, obtains B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature radiation sterilization, obtain self-crosslinking injection beauty packing material.
Comparative example 2 is not handled calcium citrate microencapsulation compared to embodiment 5, analysis crosslinking situation.
It is injected in nascent small white mouse back body after embodiment 1-5, comparative example 1-2 are prepared according to the method
Filling, injection volume 5ml, 10 days after tracking injection, back body variation in 30 days.As shown in table 1.
Table 1
| Number | 10th small white mouse back changes | 30th small white mouse back changes |
| Embodiment 1 | It is smooth, smooth, bright | It is smooth, smooth, bright |
| Embodiment 2 | It is smooth, smooth, bright | It is smooth, smooth, bright |
| Embodiment 3 | It is smooth, smooth, bright | It is smooth, smooth, bright |
| Embodiment 4 | It is smooth, smooth, bright | It is smooth, smooth, bright |
| Embodiment 5 | It is smooth, smooth, bright | It is smooth, smooth, bright |
| Comparative example 1 | It is smooth, out-of-flatness, bright | Smooth, out-of-flatness, dimness |
| Comparative example 2 | It is smooth, out-of-flatness, bright | Smooth, out-of-flatness, dimness |
By experimental analysis, comparative example 1 does not store A, B agent independently compared to embodiment 5, when not injecting sodium alginate from
Crosslinking, to network readezvous point matter acid, hyaluronic acid rheological characteristic is poor, makes to inject poor flatness；Comparative example 2 is compared to embodiment
5, calcium citrate microencapsulation is not handled, after injection, calcium ion release is too fast, causes poor plasticity, out-of-flatness, body dark
It is light.
Claims (7)
1. a kind of self-crosslinking injects beauty packing material, it is made of A agent and B agent, and A agent and B agent are independently stored, when injection uses
Mixing, the mass ratio that A agent, B agent mix is 1:15；Wherein, A agent is calcium citrate microcapsules；B agent is by following parts by weight
Group is grouped as：10~20 parts of sodium alginate, 5~30 parts of hyaluronic acid, 10~25 parts of collagen, 1~15 part of vitamin C, life
Manage 80~120 parts of brine.
2. a kind of self-crosslinking injects beauty packing material according to claim 1, it is characterised in that：The micro- glue of calcium citrate
The grain size of capsule is 5~20 microns.
3. a kind of self-crosslinking injects beauty packing material according to claim 1, it is characterised in that：The physiological saline is
0.9% sodium-chloride water solution.
4. a kind of any one of claim 1-3 preparation methods of self-crosslinking injection beauty packing material, it is characterised in that：Packet
Include following steps：
A, A agent is prepared：By calcium citrate miniaturization to micron order；By chitosan, acetic acid, water, emulsifier with mass ratio 3:0.1:
10：0.1 under 5000~8000rpm stirrings 10~15min obtain chitosan solution, it is then mixed with the calcium citrate of miniaturization
Even spraying drying is closed, calcium citrate microcapsules are obtained；That is A agent；
B, B agent is prepared：Sodium alginate, hyaluronic acid, collagen, vitamin C and physiological saline are mixed, stirs and evenly mixs, obtains
To B agent；
C, packaging sterilizing：A agent and B agent are packed respectively, after room temperature sterilizing, obtain self-crosslinking injection beauty packing material.
5. a kind of preparation method of self-crosslinking injection beauty packing material according to claim 4, it is characterised in that：The breast
Agent is one kind in fatty acid mono glycidol, lauric monoglyceride, sorbose alcohol ester.
6. a kind of preparation method of self-crosslinking injection beauty packing material according to claim 4, it is characterised in that：The lemon
The weight of lemon acid calcium and the volume ratio of chitosan solution are 1~3g:10mL.
7. a kind of preparation method of self-crosslinking injection beauty packing material according to claim 4, it is characterised in that：Step c
The sterilizing is radiation sterilization.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109224127A (en) * | 2018-12-04 | 2019-01-18 | 上海其胜生物制剂有限公司 | A kind of self assembly collagen stimulation microballoon of the shell-core structure naturally formed and preparation method thereof |
| CN111978600A (en) * | 2020-09-02 | 2020-11-24 | 山东华熙海御生物医药有限公司 | Hyaluronic acid composite gel for pacifying nipple |
| CN113769175A (en) * | 2021-10-09 | 2021-12-10 | 上海朵凌医疗科技有限公司 | Composition containing sodium alginate and polylactic acid as well as preparation method and application thereof |
| CN113877000A (en) * | 2021-10-13 | 2022-01-04 | 科笛生物医药(无锡)有限公司 | Microsphere composition for injection and application thereof |
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2018
- 2018-04-18 CN CN201810348070.4A patent/CN108465124A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109224127A (en) * | 2018-12-04 | 2019-01-18 | 上海其胜生物制剂有限公司 | A kind of self assembly collagen stimulation microballoon of the shell-core structure naturally formed and preparation method thereof |
| CN109224127B (en) * | 2018-12-04 | 2021-02-02 | 上海其胜生物制剂有限公司 | Self-assembled collagen stimulation microsphere with naturally-composed shell-core structure and preparation method thereof |
| CN111978600A (en) * | 2020-09-02 | 2020-11-24 | 山东华熙海御生物医药有限公司 | Hyaluronic acid composite gel for pacifying nipple |
| CN113769175A (en) * | 2021-10-09 | 2021-12-10 | 上海朵凌医疗科技有限公司 | Composition containing sodium alginate and polylactic acid as well as preparation method and application thereof |
| CN113877000A (en) * | 2021-10-13 | 2022-01-04 | 科笛生物医药(无锡)有限公司 | Microsphere composition for injection and application thereof |
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