CN101933620A

CN101933620A - Probiotic powder and preparation method

Info

Publication number: CN101933620A
Application number: CN2009100884571A
Authority: CN
Inventors: 何黎明; 李朗
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-07-01
Filing date: 2009-07-01
Publication date: 2011-01-05

Abstract

The invention provides dry probiotic powder. The probiotic powder is a probiotic mixture of microzyme, lactic acid bacteria and photosynthetic bacteria, wherein the mixing ratio of the microzyme, the lactic acid bacteria to the photosynthetic bacteria is 1-2:1-3:1; the total plate count is 1.5*109 to 1.2*1,010cfu/g; and the average grain size of the probiotic powder is 1 to 9um m. The invention relates to a process for culturing and preparing the probiotic mixture. A method for preparing the probiotic powder of which the average grain size is 1 to 9um m comprises the following steps of: preparing mixing probiotic culture by using three different kinds of probiotic culture solution, adding purified water, defatted milk powder, sodium bicarbonate, porous starch, defatted soybean powder, brown sugar and the like into the mixing probiotic culture, mixing and stirring the mixture, and performing propagation culture; and then performing freeze drying by using an ultralow temperature vacuum freeze drying machine to obtain the finished product. The preparation method has the advantages of simple production process and low production cost. The dry probiotic powder can be added into products such as foods and nutritional supplements and has a wide application range.

Description

A kind of probiotic powder and preparation method

Technical field

The invention belongs to the cultivation of microbial product and the technical field of production technology aspect, relate to a kind of probiotic powder and preparation method specifically.

Background technology

Present medicine and food manufacturing drying means mainly contain freeze-drying and high temperature spray-drying method, freeze-drying is to carry out drying under-20 ℃～-160 ℃ low-temperature condition, and the high temperature spray-drying method is to utilize spray dryer to carry out under hot conditions more than 100 ℃.In two kinds of methods, the former since a lot of bacteriums under the cryogenic conditions of normality, also can survive, pollution microbes easily, the latter then because higher temperature not only can be killed assorted bacterium, also can kill the purpose flora.So, how to make the purpose flora be issued to bigger value-added effect in situation about not being bacterial contamination, improve its activity, amplify its performance, be the problem demanding prompt solution that the present microorganism production field faces jointly.In addition, aspect the seed selection of bacterial classification, also there is certain degree of difficulty in the research and development of Mixed Microbes, and the single meeting of bacterial classification causes function not strong, and value is little, but the Mixed Microbes kind is too much, can't guarantee that then flora itself can not impact goods because of antagonism.Thereby performance is good, function is complete, stay-in-grade probiotic composition if can produce, and so, a difficult problem that runs in many medicine and the food processing process all can be readily solved, and also has immeasurable effect for the quality of life that improves people.

Summary of the invention

The object of the present invention is to provide a kind of mixing probiotic powder and preparation method.

For achieving the above object; mixing probio provided by the invention includes saccharomycete, lactic acid bacteria and photosynthetic bacteria; its strain number ratio is 1-2: 1-3: 1; total viable count is 1.5*109～2.0*1010 in this mixing probio; by average grain diameter is that the coccoid probiotics bacterial powder of 1 μ m～9 μ m constitutes, and each powder all has embedding and the carbohydrate protectant double protection of porous-starch to the bacterium powder.

The preparation method of mixing probiotic composition is with mixed-culture medium, skimmed milk power, porous-starch, defatted soy flour, the brown sugar of probio, adds purified water and mix, under 42 ℃ incubator condition, after breeding in 48 hours is cultivated, carry out freeze drying with the vacuum freeze drier below-20 ℃, the production average grain diameter is the coccoid probiotics bacterial powder of 1 μ m～9 μ m.

The specific embodiment

The preparation method of this mixing probiotic composition is with the mixed-culture medium of probio (weight ratio) 2.5%～3.0%, skimmed milk power 4%～5%, mineral salt 0.8%, sodium acid carbonate 1%, porous-starch 3%～5%, defatted soy flour 5%～7%, brown sugar 1%～2%, adds purified water to 100% and mix, under 42 ℃ incubator condition, after breeding in 48 hours is cultivated, carry out freeze drying with the vacuum freeze drier below-20 ℃, the production average grain diameter is the coccoid probiotics bacterial powder of 1 μ m～9 μ m.

Advantage of the present invention is that the living bacterium rate of this mixing probiotic composition is very high, and multiple functional, nutritional sufficiency, and be the powder of superfine microcell, it can be added in the food replenishers, and can be used as the Core Feature composition in the feed addictive as healthy food and nutriment.In addition, owing to the double protection of this powder by porous-starch and carbohydrate, its acid resistance, low temperature resistant and good salt tolerance, but long preservation is used.Low, the various problems of utilizing manufacturing process of the present invention to can be good at solving to run in the traditional mode of production process such as afunction is serious, quality instability of living bacterium rate, use better simply production equipment, efficiently, stable continuous produces purity height, probio powder that living bacterium rate is high, promptly mixes probiotic composition.

Experimental data

Discussion of results

The present invention by changing the trophic function and the component content of bacterium culture medium, can obviously improve the living environment of bacterial classification, and can be good at protecting bacterial classification not to be subjected to Effect of Environmental such as temperature, pH value under certain conditions.

The adding of porous-starch can make the bacterial classification embedding wherein, has reduced thalline and has contacted with the degree of depth of air and chamber wall; The application of carbohydrate can make the thalline in the starch hole be in the airtight relatively environment; not only reduced the influence that causes because of catching a cold; can also well protect thalline because of installing operating noise and other extraneous factors to its influence that causes; thereby the quantity that makes thalline in the bacterium powder can guarantee a higher level; reach the maximization of effect; and the freezing preservation of ultra low temperature vacuum not only can be eliminated the pollution of assorted bacterium; the one-tenth live time that also can keep bacterial classification; increase the thalline survival rate greatly, thereby make this product can accomplish long, steady quality of holding time.In addition, the corresponding improvement owing to equipment makes product quality more stable.Based on above all advantages, the present invention will bring revolutionary change to the market of traditional pharmacy, feed, health products and related industry, and market prospects are wide.

Claims

1. the dry bacterium powder of probio is the mixing probio of saccharomycete, lactic acid bacteria and photosynthetic bacteria; Wherein, the bacterial classification quantitative proportion of saccharomycete, lactic acid bacteria and photosynthetic bacteria is 1-2: 1-3: 1, and its total aerobic plate count is 1.5 * 10 ⁹～1.2 * 10 ¹⁰Cfu/g, the average grain diameter of bacterium powder is 1 μ m～9 μ m.

2. a method for preparing the dry bacterium powder of the described probio of claim 1 is utilized embedding and the carbohydrate protectant duplicate protection of porous-starch to the bacterium powder, makes it reduce loss in the process of ultralow temperature drying; Its key step is:

The probio pure culture liquid of saccharomycete, lactic acid bacteria and photosynthetic bacteria is made the probio mixed-culture medium, in mixed-culture medium, add by weight: skimmed milk power 4%～5%, mineral salt 0.8%, sodium acid carbonate 1%, porous-starch 3%～5%, defatted soy flour 5%～7%, brown sugar 1%～2%, adding purified water to 100% mixes, under 35-42 ℃ incubator condition, after breeding in 36-48 hour is cultivated, carry out freeze drying with the vacuum refrigeration below-20 ℃, obtain the dry bacterium powder of probio.

3. preparation method according to claim 2, wherein, the concentration of probio mixed-culture medium is 2.5%～3.0% by weight.

4. preparation method according to claim 2, wherein, vacuum refrigeration is to adopt vacuum freeze drier to carry out.