CN101857879A - Method for promoting and inducing Pleurotus eryngii mycelium to produce and accumulate triterpenoid - Google Patents
Method for promoting and inducing Pleurotus eryngii mycelium to produce and accumulate triterpenoid Download PDFInfo
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- CN101857879A CN101857879A CN201010158346A CN201010158346A CN101857879A CN 101857879 A CN101857879 A CN 101857879A CN 201010158346 A CN201010158346 A CN 201010158346A CN 201010158346 A CN201010158346 A CN 201010158346A CN 101857879 A CN101857879 A CN 101857879A
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Abstract
The invention relates to method for stimulating and controlling the environmental factors for the growth of Pleurotus eryngii and for promoting and inducing the Pleurotus eryngii mycelium to produce and accumulate triterpenoid. By controlling different light medium, lighting time, time difference variation, humidity variation and the content of carbon dioxide to stimulate and induce the Pleurotus eryngii mycelium to produce and accumulate the triterpenoid. The content of the triterpenoid in the obtained Pleurotus eryngii mycelium reaches 3.1 to 4.6 percent.
Description
Technical field
The present invention relates to a kind of Pleurotus eryngii and grow and give that environmental factors stimulates and regulation and control, promote and induce the Pleurotus eryngii mycelium to produce and the method for accumulation triterpene compound.
Background technology
Pleurotus eryngii (Pleu rotus eryngii Quei) is under the jurisdiction of Holobasidiomycetes, Agaricales, Pleurotaceae, pleurotus in the mycology classification.The nutritious equilibrium of Pleurotus eryngii is a kind of high protein, low-fat nutritive health-care food.Contain 18 seed amino acids.Wherein eight kinds is that human body must amino acid, in the dried according to surveying and determination mushroom crude protein content up to 25%, fat 1.4%, robust fibre 6.9%, ash content 6.9%, vitamin c 7.6mg, and mineral elements such as calcium, phosphorus, iron.And contain the polysaccharide that can improve body immunity.Modern medicine makes substantial progress to analysis of effective component, pharmacology and the clinical application research of Pleurotus eryngii, pharmacological action and mechanism of action to the Pleurotus eryngii extract carry out big quantity research, particularly the Pleurotus eryngii triterpene compound is applied to the clinical efficacy of tumor chemoradiotherapy assisting therapy, enjoys Chinese scholars to attract attention.But it is extremely low that the Pleurotus eryngii that physical environment is grown down contains triterpene compound content.
Summary of the invention
The purpose of this invention is to provide and a kind ofly give at the Pleurotus eryngii growth and development stage that environmental factors stimulates and regulation and control, promote and induce the generation of Pleurotus eryngii secondary metabolite triterpene compound and the method for accumulation.
The objective of the invention is to give environmental factors stimulation and regulation and control, comprise by regulation and control different light medium and light application time, difference variation, humidity variation and CO at the growth and development stage of Pleurotus eryngii
2These environmental factorss of content stimulate the generation well accumulation of inducing Pleurotus eryngii mycelium meta-bolites triterpene compound; The contained triterpene compound content of the Pleurotus eryngii mycelium that is obtained is up to 3.1-4.6%.
The concrete steps of method of the present invention are as follows:
1, a kind of method that promotes and induce Pleurotus eryngii generation and accumulation triterpene compound is characterized in that the concrete steps of this method are as follows:
(1) medical stone of 0.001-0.3 part that adding medical stone ore deposit, Xinfeng County produces in culture medium preparation, medical stone grinding particle size 100-300 order:
(2) send out the bacterium stage, adopt the illumination of blue scattering half-light, intensity of illumination is the 100-900 Lux;
(3) urge the flower bud stage, adopt the illumination of champac scattering half-light, intensity of illumination is the 100-900 Lux, and control mushroom room room temps is that control space, mushroom room humidity is about 90-95%, and fills the air CO in the regulation and control Pleurotus eryngii raising chamber
2Concentration reaches 1000-4000ppm;
(4) the management of producing mushroom stage, control mushroom room room temps is that control space, mushroom room humidity is about 80-90%, and fills the air CO in the regulation and control Pleurotus eryngii raising chamber
2Concentration reaches 1000-4000ppm.
The inventive method is on the basis of the method for conventional factory culture Pleurotus eryngii, reaches purpose of the present invention by above-mentioned technique improvement.Test of the present invention shows that different light medium and light application time, difference variation and humidity change, CO
2The stimulation Pleurotus eryngii is grown environmental factorss such as content and the triterpene compound content presents otherness. differentiate the cap stage at Pleurotus eryngii, blue scattered light illumination is provided and prolongs light application time, produce the stage of sporidium at Pleurotus eryngii, change light quality and be black-and-blue scattered light and prolong light application time, can promote Pleurotus eryngii to produce a large amount of sporidium; Room temperature is 23-26 ℃ in the regulation and control Pleurotus eryngii cultivation booth during the Pleurotus eryngii spore of gathering, and it is vigorous to promote that the Pleurotus eryngii spore sprays; In the Pleurotus eryngii sporophore stage of maturity, in the regulation and control Pleurotus eryngii nurturing an environment round the clock between temperature difference amplitude, humidity and CO
2Content also forms extreme environment, can stimulate the generation well accumulation of inducing Pleurotus eryngii spore secondary metabolite triterpene compound, Pleurotus eryngii spore quality significantly improves, triterpene compound content is up to 3.1-4.6% in the Pleurotus eryngii spore, through further processing, can be used as tumor chemoradiotherapy medicine and adjuvant therapy medicaments and be applied to clinical.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one
1, the medical stone of 0.001 weight percent that adding medical stone ore deposit, Xinfeng County produces in culture medium preparation, medical stone grinding particle size 100 orders;
2, send out the bacterium stage, adopt the illumination of blue scattering half-light, intensity of illumination is 800 Luxs;
3, urge the flower bud stage, adopt the illumination of champac scattering half-light, intensity of illumination is 800 Luxs, and control mushroom room room temps is that control space, mushroom room humidity is about 90-95%, and fills the air CO in the regulation and control Pleurotus eryngii raising chamber
2Concentration reaches 1000-4000ppm;
4, the management of producing mushroom stage, control mushroom room room temps is that control space, mushroom room humidity is about 80-90%, and fills the air CO in the regulation and control Pleurotus eryngii raising chamber
2Concentration reaches 1000-4000ppm.
5, during the Pleurotus eryngii of gathering, in the regulation and control Pleurotus eryngii raising chamber round the clock room temperature be 23-26 ℃, the fresh Pleurotus eryngii on the same day was in time gathered at dusk and dry on the same day.By analysis, the contained triterpene compound content of the Pleurotus eryngii spore of results is 4.3-4.5%.
Claims (1)
1. one kind promotes and induces Pleurotus eryngii to produce and accumulate the method for triterpene compound, it is characterized in that the concrete steps of this method are as follows:
(1) medical stone of 0.001-0.3 part that adding medical stone ore deposit, Xinfeng County produces in culture medium preparation, medical stone grinding particle size 100-300 order:
(2) send out the bacterium stage, adopt the illumination of blue scattering half-light, intensity of illumination is the 100-900 Lux;
(3) urge the flower bud stage, adopt the illumination of champac scattering half-light, intensity of illumination is the 100-900 Lux, and control mushroom room room temps is that control space, mushroom room humidity is about 90-95%, and fills the air CO in the regulation and control Pleurotus eryngii raising chamber
2Concentration reaches 1000-4000ppm;
(4) the management of producing mushroom stage, control mushroom room room temps is that control space, mushroom room humidity is about 80-90%, and fills the air CO in the regulation and control Pleurotus eryngii raising chamber
2Concentration reaches 1000-4000ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201010158346A CN101857879A (en) | 2010-03-16 | 2010-03-16 | Method for promoting and inducing Pleurotus eryngii mycelium to produce and accumulate triterpenoid |
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| CN201010158346A CN101857879A (en) | 2010-03-16 | 2010-03-16 | Method for promoting and inducing Pleurotus eryngii mycelium to produce and accumulate triterpenoid |
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| CN101857879A true CN101857879A (en) | 2010-10-13 |
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| CN201010158346A Pending CN101857879A (en) | 2010-03-16 | 2010-03-16 | Method for promoting and inducing Pleurotus eryngii mycelium to produce and accumulate triterpenoid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013035473A1 (en) * | 2011-09-07 | 2013-03-14 | 国立大学法人信州大学 | Method for producing useful metabolite from filamentous fungus |
| RU2488989C1 (en) * | 2012-06-20 | 2013-08-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Уральская государственная академия ветеринарной медицины" | Method of stimulating the growth of oyster mushrooms |
| CN104126412A (en) * | 2014-05-09 | 2014-11-05 | 江南大学 | Method for promoting growth of antrodia cinnamomea liquid-state fermentation mycelium and high yields of terpenoid compounds by adding terpineol |
| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| CN109182141A (en) * | 2018-09-25 | 2019-01-11 | 中国农业科学院麻类研究所 | A method of improving white-rot fungi producing enzyme enzyme activity |
| CN110226450A (en) * | 2019-06-12 | 2019-09-13 | 肇庆绿健农业科技有限公司 | A kind of breeding method of the brave matsutake of bottle of cultivation |
-
2010
- 2010-03-16 CN CN201010158346A patent/CN101857879A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013035473A1 (en) * | 2011-09-07 | 2013-03-14 | 国立大学法人信州大学 | Method for producing useful metabolite from filamentous fungus |
| CN103930556A (en) * | 2011-09-07 | 2014-07-16 | 国立大学法人信州大学 | Method for producing useful metabolite from filamentous fungus |
| JPWO2013035473A1 (en) * | 2011-09-07 | 2015-03-23 | 国立大学法人信州大学 | Method for producing useful metabolites from filamentous fungi |
| CN103930556B (en) * | 2011-09-07 | 2016-03-30 | 国立大学法人信州大学 | From the method for thread fungus production desirable metabolites |
| US9834796B2 (en) | 2011-09-07 | 2017-12-05 | Shinshu University | Method for producing useful metabolite from filamentous fungus |
| RU2488989C1 (en) * | 2012-06-20 | 2013-08-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Уральская государственная академия ветеринарной медицины" | Method of stimulating the growth of oyster mushrooms |
| CN104126412A (en) * | 2014-05-09 | 2014-11-05 | 江南大学 | Method for promoting growth of antrodia cinnamomea liquid-state fermentation mycelium and high yields of terpenoid compounds by adding terpineol |
| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| CN109182141A (en) * | 2018-09-25 | 2019-01-11 | 中国农业科学院麻类研究所 | A method of improving white-rot fungi producing enzyme enzyme activity |
| CN110226450A (en) * | 2019-06-12 | 2019-09-13 | 肇庆绿健农业科技有限公司 | A kind of breeding method of the brave matsutake of bottle of cultivation |
| CN110226450B (en) * | 2019-06-12 | 2021-12-28 | 向国忠 | Cultivation method of bottle-cultivated tiger tricholoma matsutake |
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Application publication date: 20101013 |