CN101828514A - Quick germination method of grass wrack seeds - Google Patents
Quick germination method of grass wrack seeds Download PDFInfo
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- CN101828514A CN101828514A CN200910223107A CN200910223107A CN101828514A CN 101828514 A CN101828514 A CN 101828514A CN 200910223107 A CN200910223107 A CN 200910223107A CN 200910223107 A CN200910223107 A CN 200910223107A CN 101828514 A CN101828514 A CN 101828514A
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Abstract
The invention discloses a quick germination method of grass wrack seeds. The method comprises the following steps of: after the grass wrack seeds are washed clean, directly putting the grass wrack seeds into 0.5 percent low-salinity artificial seawater; putting the 0.5 percent low-salinity artificial seawater with the soaked grass wrack seeds into an incubator in the presence of constant temperature and illumination for culturing for 3 weeks to ensure that the grass wrack seeds germinate, wherein the holding temperature in the presence of the constant temperature and illumination is 12-16 DEG C, the illumination intensity is 40 mu molm<-2>s<-1> and the ratio of L:D is 12:12; leading the germinated seeds into a salinity raising and domestication stage: ensuring that the salinity of the artificial seawater for soaking the germinated grass wrack seeds is improved by 0.5 percent per day until reaching a normal seawater salinity of 3 percent; and then sowing the germinated grass wrack seedlings adapting to the natural seawater salinity in an appointed sea field. The method can ensure that the grass wrack seeds germinate quickly and completely and normally grow so that the probability of suffering from being swallowed by seed-eating organisms of the grass wrack seeds is greatly reduced and is beneficial to the recovery and reconstruction of sea grass meadows so as to quickly recover good marine ecological environment.
Description
Technical field
The present invention relates to the breeding method of grass wrack seeds, relate in particular to the quick germination method of grass wrack seeds.
Background technology
Zostera marina (Zostera marina L.) is commonly called as the sea-tangle grass, belong to Angiospermae (Angiospermae), Monocotyledonae (Monocotyledoneae, or title Liliopsida Liliopsida), Alismatales (Alismales), Potamogetonaceae (Potamogetonaceae), Zostera marina belongs to (Zostera L.), perennial herb, root-like stock is crawled, and gives birth to fibrous root on the joint.Stem is dredged branch estranged, and is flat, light green.The leaf alternate, green, strip reaches more than 50 centimetres, and is wide about 3～5 millimeters, the top circle, full edge has 5～7 arteries and veins, and stipule is membranous.Spadix is wrapped in the spathe at the beginning, and long 4～6 centimetres, rhachis is flat, and the edge does not have the bracteal adjunct; Spend little, unisexuality, female, male flower is lined up 2 row, no perianth mutually along rhachis one top-cross; Ovary ovum shape oblong; Column cap 2, setiform.Seed square circle is about 4 millimeters, and longitudinal grin is arranged.Zostera marina is distributed in provinces such as the Shandong, Hebei, Liaoning of China; And be distributed in countries and regions such as Korea, Japan, Europe, North America.Zostera marina is born in the seabeach, it has high marine ecology and is worth, the large tracts of land Zostera marina forms " sea grass bed ", can be part economic fish and many marine organisms good habitat and spawning ground is provided, and can purifying sea water, to reducing the eutrophication of marine environment, reduce red tide and take place, safeguard the coastal ocean fisheries environment, keep the marine ecology balance and have important value.Because the minimizing and the decline of Zostera marina natural resources are carried out the Zostera marina manual reversion and are become one of research focus of marine environmental protection.Utilizing the grass wrack seeds of collecting to carry out the sea grass bed reconstruction is to carry out one of important technical of sea grass bed recovery.But grass wrack seeds has the characteristic of dormancy under adverse environment, and dormancy time was 1 year.Sea grass bed reconstruction at present is directly to sow or to send out the sea area after the grass wrack seeds collection, because grass wrack seeds has hibernation feature and can not enter germinating immediately, make grass wrack seeds between resting stage, though can spend unfavorable sprouting environment smoothly, but also increase the probability of eating of being eaten the seed biology simultaneously, in addition, the heterogeneity of grass wrack seeds dormancy, seed germination can not be carried out synchronously, thereby cause a large amount of losses of seed, the quantity that the grass wrack seeds under nature finally can be sprouted and survive is very low.At present; the domestic mechanism that sprouting is studied to grass wrack seeds seldom; research institution of only a few family attempts grass wrack seeds and carry out sprouting test in nature seawater (natural seawater salinity is 30 ‰); this technological deficiency is: sprouting speed is very slow; need time several months to sprout; Chang hatching and sprout time have increased the probability of grass wrack seeds loss like this; having reduced seed to a certain extent effectively sprouts; increase cost and labour intensity that sea grass bed is rebuild, do not reached the purpose of grass wrack seeds fast-germination.
Summary of the invention
The objective of the invention is to overcome existing grass wrack seeds and sprout asynchronously, sprout slowly under the nature, influence the difficult problem that sea grass bed is rebuild efficient, provide a kind of grass wrack seeds that makes to sprout rapidly and the breeding method of complete normal development.
The technical scheme that the present invention solves the problems of the technologies described above is:
A kind of quick germination method of grass wrack seeds the steps include:
(1) with artificial seawater plain and pure water to be mixed with salinity be 5 ‰ low-salinity artificial seawater;
(2) grass wrack seeds is rinsed well after, 5 ‰ the low-salinity artificial seawater of putting into directly that above-mentioned steps (1) is mixed with;
(3) above-mentioned steps (2) immersion is had 5 ‰ low-salinity artificial seawater of grass wrack seeds insert interior cultivation of constant temperature illumination box and 3 weeks made its sprouting, this constant temperature illumination keeps 12 ℃～16 ℃ of temperature, intensity of illumination 40 μ mol m
-2s
-1, L: D=12: 12;
(4) seed of Meng Faing enters the salinity rising domestication stage: make the artificial seawater salinity of the grass wrack seeds that immersion sprouted improve 5 ‰ every day, until the normal seawater salinity that reaches 30 ‰;
(5) sprouted and the Zostera marina seedling that has adapted to natural seawater salinity in the Hai Chang of appointment sowing.
It is 14 ℃ that best constant temperature illumination in the above-mentioned steps (3) keeps temperature.
Technique effect of the present invention is: it is long that the present invention has overcome existing grass wrack seeds resting stage, sprout slow and influence the technical barrier that sea grass bed is rebuild efficient, use the inventive method, grass wrack seeds is sprouted rapidly and normal development fully, reach the purpose of grass wrack seeds fast-germination, make grass wrack seeds be eaten the probability that the seed biology eats and significantly reduce, help the restoration and reconstruction of Zostera marina sea grass bed, the fast good marine eco-environment of quick-recovery.
Description of drawings
1, Fig. 1 is a flow chart of steps of the present invention.
2, Fig. 2 is grass wrack seeds different time seed germination state photo in the low salinity seawater of salinity 5 ‰.
3, Fig. 3 is grass wrack seeds different time seed germination state photo in the nature seawater of salinity 30 ‰.
Embodiment
Embodiment 1
As shown in Figure 1, a kind of ocean quick germination method of grass wrack seeds the steps include:
(1) with artificial seawater plain and pure water to be mixed with salinity be 5 ‰ low-salinity artificial seawater.
(2) it is complete to select shape, and quality is hard, full grains, and grass wrack seeds ripe and that have a vigor is directly put into the 5 ‰ low-salinity artificial seawater that above-mentioned (1) is mixed with after rinsing well.When grass wrack seeds was used pure water rinsing, up to not having tangible attachment and impurity to get final product, generally the temperature remains within the normal range gets final product for the water temperature of flushing pure water.
(3) above-mentioned steps (2) immersion is had 5 ‰ low-salinity artificial seawater of grass wrack seeds insert interior cultivation of constant temperature illumination box and 3 weeks made its sprouting, this constant temperature illumination keeps 14 ℃ of temperature, intensity of illumination 40 μ mol m
-2s
-1, L: D=12: 12.L: D=12 wherein: 12 be light application time than interlunation, i.e. illumination 12 hours, dark 12 hours, simulation was switched round the clock.Grass wrack seeds is sprouted fully after 3 weeks.
(4) grass wrack seeds of Meng Faing enters the salinity rising domestication stage: make the artificial seawater salinity of the grass wrack seeds that immersion sprouted improve 5 ‰ every day, until the normal seawater salinity that reaches 30 ‰, that is:
The grass wrack seeds that is immersed in the artificial seawater of salinity 5 ‰ and sprouted being put into the artificial seawater of salinity 10 ‰ cultivated 1 day;
The grass wrack seeds that is immersed in the artificial seawater of salinity 10 ‰ and sprouted being put into the artificial seawater of salinity 15 ‰ cultivated 1 day;
The grass wrack seeds that is immersed in the artificial seawater of salinity 15 ‰ and sprouted being put into the artificial seawater of salinity 20 ‰ cultivated 1 day;
The grass wrack seeds that is immersed in the artificial seawater of salinity 20 ‰ and sprouted being put into the artificial seawater of salinity 25 ‰ cultivated 1 day;
The grass wrack seeds that is immersed in the artificial seawater of salinity 25 ‰ and sprouted is put into the artificial seawater of salinity 30 ‰ (salinity of nature seawater) and cultivated 1 day, at this moment grass wrack seeds adapts to 30 ‰ natural seawater salinity fully;
(5) sprout fully and the Zostera marina seedling that has adapted to natural seawater salinity 30 ‰ in the Hai Chang of appointment sowing.
The purpose of " salinity raise domestication " program is can cause the dehydration of Zostera marina seedling for the Zostera marina seedling that prevents to be in low salinity directly enters high salinity from low salinity, thereby causes the seedling damage." salinity raises and the tames " program that the present invention relates to is that the grass wrack seeds that germinates and sprout that is in low salinity is transferred to than in the high salinity artificial seawater, soak cultivation according to grass wrack seeds from the order of salinity 5 ‰ → salinity 10 ‰ → salinity 15 ‰ → salinity 20 ‰ → salinity 25 ‰ → salinity 30 ‰, salinity raise once in per 24 hours, and each salinity difference is 5 ‰.Must be consistent with the artificial seawater temperature that the Zostera marina seedling originally be in low salinity than the artificial seawater temperature of high salinity, prevent the temperature acute variation and the Zostera marina seedling is caused damage.
Contrast experiment: Fig. 2 and Fig. 3 are that two grass wrack seeds experimental group are that 5 ‰ artificial seawaters and salinity are the germinating contrast photo of 30 ‰ nature seawater in salinity respectively.
Fig. 2 is the germinating figure of 5 ‰ artificial seawaters for the experimental group grass wrack seeds in salinity, the state photo when wherein A figure (Fig. 2 the picture left above) has just put into culture dish for grass wrack seeds; B figure (Fig. 2 top right plot) is the germinating photo of grass wrack seeds after through 1 week; C figure (Fig. 2 lower-left figure) is the germinating photo of grass wrack seeds after through 2 weeks, and at this moment the Zostera marina cotyledon has grown to more than 1 centimetre; D figure (Fig. 2 bottom-right graph) is a grass wrack seeds through the germinating photo after 1 month, and at this moment the Zostera marina cotyledon has grown to more than 3.5 centimetres.
Fig. 3 is the germinating figure of 30 ‰ nature seawater in salinity for the experimental group grass wrack seeds, and wherein a figure (the left figure of Fig. 2) is the state photo of grass wrack seeds when just having put into culture dish; B figure (the right figure of Fig. 2) is a grass wrack seeds through the state photo after 1 month, does not also see the rudiment sign in 1 month at the grass wrack seeds of 30 ‰ natural seawater salinity as can be seen.
Contrast as can be known by Fig. 2 and Fig. 3, the invention has the advantages that and to make the grass wrack seeds that is in hibernation shorten dormancy time greatly, even stopped resting stage, and directly enter the sprouting stage, and realize the seed germination synchronization, make grass wrack seeds enter the budding stage as early as possible, thereby continue to grow, grow, having utilized fast, the seedling of rudiment carries out effective survival rate that the artificial constructed meeting of Zostera marina sea grass bed improves structure efficient and Zostera marina greatly.
The above-mentioned salinity of preparing respectively is 5 ‰, 10 ‰, 15 ‰, 20 ‰, 25 ‰, 30 ‰ artificial seawater, and the compound method of concrete salinity artificial seawater can be with reference to following formula:
In the formula: S is the salinity value, and m is the weight that needs to add the artificial seawater element in the middle of 1000 ml pure waters, unit: gram, and promptly adding weight in 1000 ml pure waters is the artificial seawater element of m gram, the gained seawater salinity is S ‰.Because 1 ml pure water equals 1 gram, so:
Promptly in the middle of 1000 parts by weight of purified water, add 5.025 weight portion artificial seawater elements, be mixed with salinity and be 5 ‰ artificial seawater;
In the middle of 1000 parts by weight of purified water, add 10.101 weight portion artificial seawater elements, be mixed with salinity and be 10 ‰ artificial seawater;
In the middle of 1000 parts by weight of purified water, add 15.228 weight portion artificial seawater elements, be mixed with salinity and be 15 ‰ artificial seawater;
In the middle of 1000 parts by weight of purified water, add 20.408 weight portion artificial seawater elements, be mixed with salinity and be 20 ‰ artificial seawater;
In the middle of 1000 parts by weight of purified water, add 25.641 weight portion artificial seawater elements, be mixed with salinity and be 25 ‰ artificial seawater;
In the middle of 1000 parts by weight of purified water, add 30.928 weight portion artificial seawater elements, be mixed with salinity and be 30 ‰ artificial seawater.
The artificial seawater element is commonly called as artificial seawater salt, and the composition of artificial seawater element is near the composition of nature seawater, during use, closes as long as the artificial seawater element converted according to certain ratio and pure water, just can be made into the artificial seawater more approaching with natural sea-water.Its composition is made up of sodium chloride, magnesium sulfate, potassium chloride and tens kinds of trace elements.The artificial seawater element can be buied on market, as instant ocean (Instant Ocean) the artificial seawater element of U.S. Gentle Castle Corp. (Aquarium Systems Company) production; Tropical ocean (Tropic Marin) the artificial seawater element that the tropical ocean company of Germany (Tropic Marin Sea Salt Company) produces; " coral board " instant sea salt (Instant Sea Salt) artificial seawater element that strand, Weifang big waves sea crystal factory produces; And the big seawater in general sea, Qingdao have blue precious deposits (Blue Treasure) the artificial seawater element that limit company produces.The artificial seawater element of above-mentioned brand all can satisfy instructions for use of the present invention.
The used pure water of present embodiment is the pure water of water purification machine process for producing, and the used water purifior of present embodiment is that the model that Qingdao Zi Quan Instr Ltd. produces is the water purifior of FMN1001-RO, and the source water of present embodiment water purifior is the municipal tap water.
The used constant temperature illumination box of present embodiment is the illumination box that Shanghai Yiheng Scientific Instruments Co., Ltd produces, its model is: MGC-250, this constant temperature illumination box can the simulating nature illumination condition, under certain illumination and temperature conditions, can automatically switch round the clock.
Embodiment 2
Keep temperature to be set at 12 ℃ the constant temperature illumination in embodiment 1 step (3), other step and condition of culture are with embodiment 1.
Embodiment 3
Keep temperature to be set at 16 ℃ the constant temperature illumination in embodiment 1 step (3), other step and condition of culture are with embodiment 1.
Claims (2)
1. a quick germination method of grass wrack seeds is characterized in that, the steps include:
(1) with artificial seawater plain and pure water to be mixed with salinity be 5 ‰ low-salinity artificial seawater;
(2) grass wrack seeds is rinsed well after, 5 ‰ the low-salinity artificial seawater of putting into directly that above-mentioned steps (1) is mixed with;
(3) above-mentioned steps (2) immersion is had 5 ‰ low-salinity artificial seawater of grass wrack seeds insert interior cultivation of constant temperature illumination box and 3 weeks made its sprouting, this constant temperature illumination keeps 12 ℃～16 ℃ of temperature, intensity of illumination 40 μ mol m
-2s
-1, L: D=12: 12;
(4) seed of Meng Faing enters the salinity rising domestication stage: make the artificial seawater salinity of the grass wrack seeds that immersion sprouted improve 5 ‰ every day, until the normal seawater salinity that reaches 30 ‰;
(5) sprouted and the Zostera marina seedling that has adapted to natural seawater salinity in the Hai Chang of appointment sowing.
2. according to the described a kind of quick germination method of grass wrack seeds of claim 1, it is characterized in that: the constant temperature illumination in the described step (3) keeps 14 ℃ of temperature.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102577699A (en) * | 2012-01-01 | 2012-07-18 | 山东东方海洋科技股份有限公司 | Method for cultivating zostera marina aseptic seedlings |
| CN103098695A (en) * | 2013-02-22 | 2013-05-15 | 中国水产科学研究院黄海水产研究所 | Cultivation method of zostera marina high-quality seedling plants |
| CN103098694A (en) * | 2013-02-22 | 2013-05-15 | 中国水产科学研究院黄海水产研究所 | Cultivation method of zostera marina artificial sea turfs |
| CN103548443A (en) * | 2013-11-22 | 2014-02-05 | 山东省海水养殖研究所 | Sowing method of zostera marina seeds |
| CN103609422A (en) * | 2013-11-14 | 2014-03-05 | 青岛农业大学 | Zostera marina seed multiplication method |
| CN104126494A (en) * | 2013-09-22 | 2014-11-05 | 山东大学（威海） | Zostera marina indoor long-term cultivation method and device |
| CN104429218A (en) * | 2013-09-16 | 2015-03-25 | 中国海洋大学 | Natural sea-area sowing method of zostera marina seed |
| CN111226533A (en) * | 2020-03-12 | 2020-06-05 | 岭南师范学院 | Method for germinating mallotus japonicus seeds and seedling culture method |
| CN112931178A (en) * | 2021-03-29 | 2021-06-11 | 中国水产科学研究院黄海水产研究所 | Seaweed culture system and method based on land-based greenhouse |
| CN113545288A (en) * | 2020-04-23 | 2021-10-26 | 莆田市正洋水产发展有限公司 | Method for shortening cultivation period of asparagus |
| CN113615540A (en) * | 2021-08-10 | 2021-11-09 | 中国海洋大学 | Artificial composite matrix, artificially cultured eel grass seedlings, and culture method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102577699B (en) * | 2012-01-01 | 2013-12-04 | 山东东方海洋科技股份有限公司 | Method for cultivating zostera marina aseptic seedlings |
| CN102577699A (en) * | 2012-01-01 | 2012-07-18 | 山东东方海洋科技股份有限公司 | Method for cultivating zostera marina aseptic seedlings |
| CN103098695A (en) * | 2013-02-22 | 2013-05-15 | 中国水产科学研究院黄海水产研究所 | Cultivation method of zostera marina high-quality seedling plants |
| CN103098694A (en) * | 2013-02-22 | 2013-05-15 | 中国水产科学研究院黄海水产研究所 | Cultivation method of zostera marina artificial sea turfs |
| CN103098695B (en) * | 2013-02-22 | 2014-08-06 | 中国水产科学研究院黄海水产研究所 | Cultivation method of zostera marina high-quality seedling plants |
| CN103098694B (en) * | 2013-02-22 | 2014-08-27 | 中国水产科学研究院黄海水产研究所 | Cultivation method of zostera marina artificial sea turfs |
| CN104429218A (en) * | 2013-09-16 | 2015-03-25 | 中国海洋大学 | Natural sea-area sowing method of zostera marina seed |
| CN104126494A (en) * | 2013-09-22 | 2014-11-05 | 山东大学（威海） | Zostera marina indoor long-term cultivation method and device |
| CN103609422B (en) * | 2013-11-14 | 2015-05-27 | 青岛农业大学 | Zostera marina seed multiplication method |
| CN103609422A (en) * | 2013-11-14 | 2014-03-05 | 青岛农业大学 | Zostera marina seed multiplication method |
| CN103548443A (en) * | 2013-11-22 | 2014-02-05 | 山东省海水养殖研究所 | Sowing method of zostera marina seeds |
| CN103548443B (en) * | 2013-11-22 | 2015-06-17 | 山东省海水养殖研究所 | Sowing method of zostera marina seeds |
| CN111226533A (en) * | 2020-03-12 | 2020-06-05 | 岭南师范学院 | Method for germinating mallotus japonicus seeds and seedling culture method |
| CN111226533B (en) * | 2020-03-12 | 2021-09-24 | 岭南师范学院 | Method for germinating mallotus japonicus seeds and seedling culture method |
| CN113545288A (en) * | 2020-04-23 | 2021-10-26 | 莆田市正洋水产发展有限公司 | Method for shortening cultivation period of asparagus |
| CN112931178A (en) * | 2021-03-29 | 2021-06-11 | 中国水产科学研究院黄海水产研究所 | Seaweed culture system and method based on land-based greenhouse |
| CN112931178B (en) * | 2021-03-29 | 2022-03-18 | 中国水产科学研究院黄海水产研究所 | Seaweed culture system and method based on land-based greenhouse |
| CN113615540A (en) * | 2021-08-10 | 2021-11-09 | 中国海洋大学 | Artificial composite matrix, artificially cultured eel grass seedlings, and culture method and application thereof |
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| CN101828514B (en) | 2012-06-27 |
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