CN101803681B - 低聚壳聚糖-黄芪多糖生态饲料添加剂 - Google Patents
低聚壳聚糖-黄芪多糖生态饲料添加剂 Download PDFInfo
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Abstract
本发明涉及饲料添加剂，具体的说是一种低聚壳聚糖-黄芪多糖生态饲料添加剂。由产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌组成，而后于培养基中培养，28-37℃培养60-72小时，即为饲料添加剂，按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝1-3∶1-3∶1-3∶1-3；所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖20-50g与黄芪多糖20-50g蒸馏水加至1000ml，pH6.0。121℃高压灭菌20分钟，备用。本发明的添加剂可发挥多重协同功能，保障添加剂效果，并具有效果显著、使用便捷等优点。
Description
技术领域
本发明涉及饲料添加剂，具体的说是一种低聚壳聚糖-黄芪多糖生态饲料添加剂。
背景技术
随着对微生态制剂和生物活性物质研究的不断深入，微生态制剂和活性多糖在家畜的预防保健、提高生产性能等方面的作用不断明确。由于抗生素的逐步禁用以及人们对食品安全的关注，微生态制剂、活性多糖等安全型饲料添加剂将日益发挥更大的作用。但以往单一的应用方式往往在使用上难以达到理想的效果，开发复合型添加剂将是微生态制剂和活性多糖高效应用的发展方向。
发明内容
本发明的目的在于提供一种低聚壳聚糖-黄芪多糖生态饲料添加剂。
为实现上述目的，本发明采用的技术方案为：
一种低聚壳聚糖-黄芪多糖生态饲料添加剂：由产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌组成，而后于培养基中培养28-37℃培养60-72小时，即为饲料添加剂，按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝1-3∶1-3∶1-3∶1-3；所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖20-50g与黄芪多糖20-50g蒸馏水加至1000ml，pH6.0，121℃高压灭菌20分钟，备用。
将上述所得饲料添加剂与动物日粮混合使用。将上述所得饲料添加剂与动物日粮混合后投放，以动物日粮总量的0.2-1％加入上述饲料添加剂。
本发明所具有的优点：本发明添加剂可改善家畜的健康水平，提高家畜的生产性能。同时可改善肠道菌群平衡，建立健康的肠道微生物区系；对肠道有害菌群具有抑制和杀灭作用，维持肠道健康；并且可以提高动物的免疫能力；提高饲料利用率，降低料肉比，提高生产性能；可全面替代预防性抗生素的使用。
具体实施方式
饲料添加剂：将增殖培养后的产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝1∶1∶1∶1，而后于培养基中培养28-30℃培养60小时即为饲料添加剂。
所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖20g与黄芪多糖20g蒸馏水加至1000ml。所述低聚壳聚糖(来源于江门高科量子股份有限公司)；所述黄芪多糖(购自山东曲阜圣旺植物有限公司)。
所述产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌按照常规方法在下述各自的培养基中进行增殖培养。
所述产朊假丝酵母增殖培养于马铃薯浸粉(市购)3g，葡萄糖20g，琼脂粉15g，蒸馏水加至1000ml培养基中，121℃高压灭菌20分钟，备用。
所述粪肠球菌增殖培养于葡萄糖2％，蛋白胨1％，牛肉膏1％，酵母膏0.5％，磷酸氢二钾0.2％，乙酸钠0.5％，柠檬酸铵0.2％，硫酸镁0.058％，硫酸锰0.025％，琼脂1.5-2％，pH6.5培养基中，121℃高压灭菌20分钟，备用。
所述嗜酸乳杆菌增殖培养于葡萄糖2％，蛋白胨1％，牛肉膏1％，酵母膏0.5％，磷酸氢二钾0.2％，乙酸钠0.5％，柠檬酸铵0.2％，硫酸镁0.058％，硫酸锰0.025％，琼脂1.5-2％，pH6.5培养基中，121℃高压灭菌20分钟，备用。所述枯草芽孢杆菌增殖培养于蛋白胨10g，琼脂20g，氯化钠5g，肉浸粉10g，水加至1000ml，pH7.2培养基中，121℃高压灭菌20分钟，备用。
将上述所得饲料添加剂将上述所得饲料添加剂与动物日粮混合使用对肉鸡生产性能及免疫力方面进行测定(参见表1、表2)。所得饲料添加剂投放量为动物日粮总量的0.2％。
表1添加剂对肉鸡增重的影响
可见试验组与对照组相比，肉鸡体重显著增加。
表2添加剂对免疫器官指数的影响
添加剂可显著提高免疫器官指数。
实施例2
饲料添加剂：将增殖培养后的产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝1∶2∶2∶1，而后于培养基中培养30-35℃培养72小时，即为饲料添加剂。
所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖30g与黄芪多糖40g蒸馏水加至1000ml。
将上述所得饲料添加剂与动物日粮混合使用，以动物日粮总量的1％加入上述饲料添加剂。
实施例3
饲料添加剂：将增殖培养后的产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝3∶1∶1∶1，而后于培养基中培养35-37℃培养65小时，即为饲料添加剂。
所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖40g与黄芪多糖50g蒸馏水加至1000ml。
将上述所得饲料添加剂与动物日粮混合使用，以动物日粮总量的0.5％加入上述饲料添加剂。
实施例4
饲料添加剂：将增殖培养后的产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝2∶1∶1∶1，而后于培养基中培养32-34℃培养70小时，即为饲料添加剂。
所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖50g与黄芪多糖30g蒸馏水加至1000ml。
将上述所得饲料添加剂与动物日粮混合使用，以动物日粮总量的0.8％加入上述饲料添加剂。
Claims (3)
1.一种低聚壳聚糖-黄芪多糖生态饲料添加剂，其特征在于：由产朊假丝酵母、粪肠球菌、嗜酸乳杆菌和枯草芽孢杆菌组成，而后于培养基中培养28-37℃培养60-72小时，即为饲料添加剂，按重量份数计产朊假丝酵母∶粪肠球菌∶嗜酸乳杆菌∶枯草芽孢杆菌＝1-3∶1-3∶1-3∶1-3；所述培养基为琼脂1g、牛肉膏1g、酵母浸粉2.5g、蔗糖35g、消泡剂1.5g、蛋白胨1g，低聚壳聚糖20-50g与黄芪多糖20-50g蒸馏水加至1000ml。
2.按权利要求1所述的低聚壳聚糖-黄芪多糖生态饲料添加剂，其特征在于：将上述所得饲料添加剂与动物日粮混合使用。
3.按权利要求1所述的低聚壳聚糖-黄芪多糖生态饲料添加剂，其特征在于：将上述所得饲料添加剂与动物日粮混合后投放，以动物日粮总量的0.2-1％加入上述饲料添加剂。
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| CN102578384A (zh) * | 2011-04-01 | 2012-07-18 | 北京都润科技有限公司 | 枯草芽孢杆菌结合益生协同剂进行合生发酵的应用 |
| CN103621792A (zh) * | 2013-11-29 | 2014-03-12 | 吉林省科技评估有限责任公司 | 一种含l-赖氨酸复合益生菌的饲料添加剂及其制备方法 |
| CN104171500A (zh) * | 2014-07-02 | 2014-12-03 | 中泰和（北京）科技发展有限公司 | 一种提高仔猪免疫力的母猪饲料添加剂及使用方法 |
| CN105231020A (zh) * | 2015-10-03 | 2016-01-13 | 山东百龙创园生物科技有限公司 | 一种饲料添加剂组合物及其制备方法 |
| CN108782963A (zh) * | 2018-06-14 | 2018-11-13 | 河南省帝方生物制药有限公司 | 一种可抗菌提高免疫力的微生态制剂及其制备方法 |
| CN113577112B (zh) * | 2021-09-14 | 2022-03-01 | 中国农业科学院兰州畜牧与兽药研究所 | 一种防治禽沙门氏菌病的药物组合物及制备方法 |
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| US3271266A (en) * | 1962-12-31 | 1966-09-06 | British Petroleum Co | Process for cultivating microorganisms on a hydrocarbon feedstock employing a carbohydrate pretreatment feedstock |
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