CN103743877A - Dynamic exposure device for daphnia detection - Google Patents
Dynamic exposure device for daphnia detection Download PDFInfo
- Publication number
- CN103743877A CN103743877A CN201310719252.5A CN201310719252A CN103743877A CN 103743877 A CN103743877 A CN 103743877A CN 201310719252 A CN201310719252 A CN 201310719252A CN 103743877 A CN103743877 A CN 103743877A
- Authority
- CN
- China
- Prior art keywords
- passage
- substrate
- magna
- chamber
- ring seal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
Abstract
The invention relates to a dynamic exposure device for daphnia detection. The dynamic exposure device comprises a support, a sealing ring fixed on the upper end of the support, an observation vessel arranged below the sealing ring and a lifting platform for placing and fixing the observation vessel, wherein the sealing ring can seal an opening formed in the upper end of the observation vessel and is provided with a water sample in-out and balancing device, so that the dynamic exposure of the daphnia by a water sample is realized. The dynamic exposure device provided by the invention has the beneficial effects that the structure is simple, the long-term dynamic exposure of the daphnia in the water sample is realized, the foundation equipment is provided for a laboratory dynamic toxicity test and environment in situ real time on-line monitoring; the dynamic exposure device is used in match with a lighting device and a photographic device so as to be convenient to obtain the collection and analysis of the daphnia movement behavior video, and the environment can be accurately detected in real time.
Description
Technical field
The present invention relates to a kind of Magna detection dynamic exposing device.
Background technology
Daphnia magna (Daphnia magna) belongs to Arthropoda, Crustachia, and Branchiopoda, moves in natural water area, belongs to flotation crustaceans animal.It has that life cycle is short, breeding is fast, economical, be conveniently easy to get, responsive and be easy in advantages such as laboratory cultures to poisonous substance, add their importance in aquatic ecosystem, thereby be all applied in many countries, become a kind of test with model organism, be widely used in Ecology toxicological test.Daphnia magna toxicological test not only can be evaluated the pollution of sediment to water environment in industrial waste water, agricultural chemicals, chemicals and water, for formulating various water quality standard, provides scientific basis, and can monitor as monitoring means the pollution of water environment.Utilize the experimental technique of Daphnia magna to be extensively subject to domestic and international many personages' attention, there is technical prospect widely.
Daphnia magna toxicity test adopts static exposure chamber to carry out conventionally, lack the online facility that dynamically exposes of laboratory and environment original position, and the use that dynamically exposes facility can be carried out experimental test more accurately, and be the prerequisite of real time on-line monitoring, therefore, the development and application of Aquatic Ecotoxicology dynamically exposes facility in the urgent need to corresponding Daphnia magna, and so dynamic exposure technology is combined with computer video and image processing techniques also can realize the robotization that Daphnia magna toxicity is tested.
Summary of the invention
The technical problem to be solved in the present invention is: based on the problems referred to above, the invention provides a kind of Magna detection dynamic exposing device.
The present invention solves the technical scheme that its technical matters adopts: a kind of Magna detection dynamic exposing device, comprise support, be fixed on the O-ring seal of support upper end, the observation ware that is installed on O-ring seal below and the lifting platform of placement and fixed observer ware, O-ring seal is by the upper end open sealing of observation ware, and O-ring seal is provided with water sample turnover and balance device.
Further, inside observation ware, the back side is provided with the first substrate, in observation ware, be provided with right baffle-plate and right baffle plate, right baffle-plate and right baffle plate are separated into left passage, middle passage and right passage by ware inner chamber, the second substrate that middle passage upper end is provided with and is divided into cushion chamber, exposes chamber, the upper surface of the second substrate and O-ring seal laminating, the first substrate and the second substrate are structure as a whole; Expose bottom, chamber and be provided with for blocking the barrier of Magna, right baffle-plate lower end offers the discharge orifice of passage in connection, left passage, and middle in-channel water sample enters left passage from discharge orifice through barrier; The second substrate middle part offers the water inlet through hole that is communicated with cushion chamber and exposes chamber, and the second substrate right side opening is provided with the spilling water passage that is communicated with cushion chamber and right passage.
Further, water sample turnover and balance device comprise be positioned at left passage top apopore, be positioned at the cushion chamber inlet opening, top of apopore homonymy and be positioned at the spillway hole of right passage top.
Further, O-ring seal middle part offer with the through hole of intaking adapt for inserting the jack of temperature sensor, temperature sensor through jack, water inlet through hole, injects and exposes chamber successively.
Further, observation ware material is glass, and O-ring seal material is silicon rubber.
Further, the material of the first substrate, the second substrate, right baffle-plate and right baffle plate is non-toxic plastic.
The invention has the beneficial effects as follows: simple in structure, can realize the long-term dynamics of Magna in water sample exposes, for the dynamic toxicological test in laboratory and environment original position real time on-line monitoring provide infrastructure device, it and lighting device, camera head are used in conjunction with, be convenient to obtain collection and the analysis of Magna motor behavior video, more accurately real-time testing environment.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is structural representation of the present invention;
Fig. 2 is vertical view of the present invention;
Fig. 3 is the vertical view of observation ware;
Fig. 4 be in Fig. 3 A-A to cut-open view;
Fig. 5 be in Fig. 3 B-B to cut-open view;
Fig. 6 be in Fig. 3 C-C to cut-open view.
Wherein: 1. support, 2. O-ring seal, 3. observation ware, 4. the first substrate, 5. right baffle-plate, 6. right baffle plate, 7. cushion chamber, 8. expose chamber, 9. the second substrate, 10. barrier, 11. water inlet through holes, 12. spilling water passages, 13. apopores, 14. inlet openings, 15. spillway holes, 16. temperature sensors, 17. jacks, 18. discharge orifices, 19. lifting platforms.
Embodiment
The invention will be further described in conjunction with specific embodiments now, and following examples are intended to illustrate the present invention rather than limitation of the invention further.
A kind of Magna detection dynamic exposing device as shown in Fig. 1～6, comprise support 1, be fixed on the lifting platform 19 of the O-ring seal 2 of support 1 upper end, the observation ware 3 that is installed on O-ring seal 2 belows and placement and fixed observer ware 3, the upper end open sealing that O-ring seal 2 will observation ware 3.Observation ware 3 materials are glass, and O-ring seal 2 materials are silicon rubber.
Observation ware 3 back sides, inner side are provided with the first substrate 4, in observation ware 3, be provided with right baffle-plate 5 and right baffle plate 6, right baffle-plate 5 and right baffle plate 6 are separated into left passage, middle passage and right passage by observation ware 3 inner chambers, the second substrate 9 that middle passage upper end is provided with and is divided into cushion chamber 7, exposes chamber 8, the upper surface of the second substrate 9 and O-ring seal 2 laminatings, the first substrate 4 and the second substrate 9 are structure as a whole.The material of the first substrate 4, the second substrate 9, right baffle-plate 5 and right baffle plate 6 is non-toxic plastic.
Expose 8 bottoms, chamber and be provided with for blocking the barrier 10 of Magna, right baffle-plate 5 lower ends offer the discharge orifice 18 of passage in connection, left passage, and middle in-channel water sample 18 enters left passage through barrier 10 from discharge orifice.
The second substrate 9 middle parts offer water inlet through hole 11, the second substrate 9 right side openings that are communicated with cushion chamber 7 and expose chamber 8 and are provided with the spilling water passage 12 that is communicated with cushion chamber 7 and right passage.
O-ring seal 2 is provided with water sample turnover and balance device.Water sample turnover and balance device comprise be positioned at left passage top apopore 13, be positioned at apopore 13 homonymies cushion chamber 7 tops inlet opening 14 and be positioned at the spillway hole 15 of right passage top.O-ring seal 2 middle parts offer with the through hole 11 of intaking adapt for inserting the jack 17 of temperature sensor 16.Temperature sensor 16, for measuring Magna in the Exposure Temperature of water sample, is convenient to maintain the isoperibol that exposes chamber 8, reduces the impact of temperature variation on Magna toxotest.
During actual use, the liquid that Magna is housed is packed into and exposed in chamber 8 from water inlet through hole 11.Expose between four spaces such as chamber 8, left passage, cushion chamber 7 and right passage and have discharge orifice 18, water inlet through hole 11, spilling water passage 12 and observation ware 3 glass inwalls to be communicated with the trickle gap between plastic baffle plate, add liquid to liquid level and flush with cushion chamber 7 lower surface.Now, Magna, exposing the interior activity in chamber 8, exposes in chamber 8 and does not stay bubble.
Inlet opening 14 water flowings, control the interior water flow in inlet opening 14, will in cushion chamber 7, be full of, and the water in cushion chamber 7 can enter from the gap between water inlet through hole 11 and temperature sensor 16 and expose chamber 8.Magna contact exposures in exposing chamber 8 with water sample, current continue through discharge orifice, 18 to enter left passage by barrier 10, then by rising pipe bottom, through apopore 13, are discharged, and form water sample and pass in and out control, the dynamic exposure of realization exposure chamber 8 interior Magna.
The flow of controlling inlet opening 14 is greater than the flow of apopore 13 all the time slightly, keep this small pressure reduction, unnecessary water sample is discharged through spillway hole 15 from spilling water passage 12, thereby, realize control and the balance of water sample turnover, guaranteeing to expose chamber 8 has the dynamic water sample of constant volume and the normal operation that Magna dynamically exposes all the time.
Because inlet opening 14 is positioned at left side, the air in water inlet pipe and cushion chamber 7 can be rushed to the spilling water passage 12 on right side by current, arrive right passage, from spillway hole 15, discharges, and guarantees the liquid environment of whole observation, does not have bubble the dynamic exposure of Magna is caused to interference.
The above-mentioned foundation desirable embodiment of the present invention of take is enlightenment, and by above-mentioned description, relevant staff can, within not departing from the scope of this invention technological thought, carry out various change and modification completely.The technical scope of this invention is not limited to the content on instructions, must determine its technical scope according to claim scope.
Claims (6)
1. a Magna detection dynamic exposing device, it is characterized in that: comprise support (1), be fixed on the O-ring seal (2) of support (1) upper end, the observation ware (3) that is installed on O-ring seal (2) below and the lifting platform (19) of placement and fixed observer ware (3), O-ring seal (2) will be observed the upper end open sealing of ware (3), and O-ring seal (2) is provided with water sample turnover and balance device.
2. Magna detection dynamic exposing device according to claim 1, it is characterized in that: the described back side, observation ware (3) inner side is provided with the first substrate (4), in observation ware (3), be provided with right baffle-plate (5) and right baffle plate (6), right baffle-plate (5) and right baffle plate (6) will observe ware (3) inner chamber be separated into left passage, middle passage and right passage, middle passage upper end is provided with and is divided into cushion chamber (7), expose second substrate (9) in chamber (8), the upper surface of the second substrate (9) and O-ring seal (2) laminating, the first substrate (4) is structure as a whole with the second substrate (9),
Expose bottom, chamber (8) and be provided with the barrier (10) for blocking Magna, right baffle-plate (5) lower end offers the discharge orifice (18) of passage in connection, left passage, and through barrier (10), from discharge orifice, (18) enter left passage to middle in-channel water sample;
In the middle part of the second substrate (9), offer and be communicated with cushion chamber (7) and the water inlet through hole (11) that exposes chamber (8), the second substrate (9) right side opening is provided with the spilling water passage (12) that is communicated with cushion chamber (7) and right passage.
3. Magna detection dynamic exposing device according to claim 1, is characterized in that: described water sample turnover and balance device comprise be positioned at left passage top apopore (13), be positioned at apopore (13) homonymy cushion chamber (7) top inlet opening (14) and be positioned at the spillway hole (15) above right passage.
4. Magna detection dynamic exposing device according to claim 1, it is characterized in that: described O-ring seal (2) middle part offer with the through hole of intaking (11) adapt for inserting the jack (17) of temperature sensor (16), temperature sensor (16) through jack (17), water inlet through hole (11), injects and exposes chamber (8) successively.
5. Magna detection dynamic exposing device according to claim 1, is characterized in that: described observation ware (3) material is glass, and O-ring seal (2) material is silicon rubber.
6. Magna detection dynamic exposing device according to claim 2, is characterized in that: the material of described the first substrate (4), the second substrate (9), right baffle-plate (5) and right baffle plate (6) is non-toxic plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310719252.5A CN103743877B (en) | 2013-12-20 | 2013-12-20 | Magna detection dynamic exposing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310719252.5A CN103743877B (en) | 2013-12-20 | 2013-12-20 | Magna detection dynamic exposing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103743877A true CN103743877A (en) | 2014-04-23 |
| CN103743877B CN103743877B (en) | 2015-09-16 |
Family
ID=50500914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310719252.5A Expired - Fee Related CN103743877B (en) | 2013-12-20 | 2013-12-20 | Magna detection dynamic exposing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103743877B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4324200A (en) * | 1980-02-08 | 1982-04-13 | Rockhill Enterprises | Method and apparatus for growing hydrozoa |
| JPH08214726A (en) * | 1995-02-08 | 1996-08-27 | Meito Suien:Kk | Water tank unit for rearing fish and shellfish |
| WO1998041862A1 (en) * | 1997-03-17 | 1998-09-24 | United States As Represented By The Secretary Of The Army | An apparatus and method for automated biomonitoring of water quality |
| JP2008134119A (en) * | 2006-11-28 | 2008-06-12 | Takahiro Yamamoto | Automatic water quality monitoring device |
| CN101201342A (en) * | 2007-12-14 | 2008-06-18 | 河海大学 | Test system for synchronizing aquatic organism characteristics statically and dynamically with the duress of pollution |
| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN201821759U (en) * | 2010-03-26 | 2011-05-11 | 上海海圣工贸有限公司 | Trickling water supply-type aquatic creature cultivation system for toxicological experiment |
| CN102495188A (en) * | 2011-12-09 | 2012-06-13 | 中国科学院烟台海岸带研究所 | Online automatic dynamic exposure and water body treatment system in laboratory |
| CN102830202A (en) * | 2012-08-22 | 2012-12-19 | 中国水产科学研究院东海水产研究所 | Closed static aquatic organism toxicity experiment device |
| CN102937637A (en) * | 2012-10-22 | 2013-02-20 | 中国环境科学研究院 | Water body experiment apparatus and method |
| CN203849255U (en) * | 2013-12-20 | 2014-09-24 | 常州市环境监测中心 | Dynamic exposure device for daphnia detection |
-
2013
- 2013-12-20 CN CN201310719252.5A patent/CN103743877B/en not_active Expired - Fee Related
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4324200A (en) * | 1980-02-08 | 1982-04-13 | Rockhill Enterprises | Method and apparatus for growing hydrozoa |
| JPH08214726A (en) * | 1995-02-08 | 1996-08-27 | Meito Suien:Kk | Water tank unit for rearing fish and shellfish |
| WO1998041862A1 (en) * | 1997-03-17 | 1998-09-24 | United States As Represented By The Secretary Of The Army | An apparatus and method for automated biomonitoring of water quality |
| JP2008134119A (en) * | 2006-11-28 | 2008-06-12 | Takahiro Yamamoto | Automatic water quality monitoring device |
| CN101201342A (en) * | 2007-12-14 | 2008-06-18 | 河海大学 | Test system for synchronizing aquatic organism characteristics statically and dynamically with the duress of pollution |
| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN201821759U (en) * | 2010-03-26 | 2011-05-11 | 上海海圣工贸有限公司 | Trickling water supply-type aquatic creature cultivation system for toxicological experiment |
| CN102495188A (en) * | 2011-12-09 | 2012-06-13 | 中国科学院烟台海岸带研究所 | Online automatic dynamic exposure and water body treatment system in laboratory |
| CN102830202A (en) * | 2012-08-22 | 2012-12-19 | 中国水产科学研究院东海水产研究所 | Closed static aquatic organism toxicity experiment device |
| CN102937637A (en) * | 2012-10-22 | 2013-02-20 | 中国环境科学研究院 | Water body experiment apparatus and method |
| CN203849255U (en) * | 2013-12-20 | 2014-09-24 | 常州市环境监测中心 | Dynamic exposure device for daphnia detection |
Non-Patent Citations (1)
| Title |
|---|
| 李辛夫: "用于水污染生物检测的恒流稀释装置的调节及计算方法", 《水生生物学报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103743877B (en) | 2015-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104880457B (en) | A kind of contactless pollutant concentration diffusion detecting system and method | |
| CN202710481U (en) | Device for identifying and measuring air bubble in high-concentration dispersed multiphase flow | |
| CN106442493A (en) | Low-concentration ammonia nitrogen detection method and device for gathered ammonia gas | |
| CN107917947A (en) | A kind of online water quality detecting device | |
| CN203849255U (en) | Dynamic exposure device for daphnia detection | |
| CN206450467U (en) | A kind of water quality monitoring pretreatment unit and water quality early-warning device | |
| CN104458656A (en) | Online turbidity meter with flow control | |
| CN109187646A (en) | A kind of microbubble and wadding body comprehensive detection system and method | |
| CN103743877B (en) | Magna detection dynamic exposing device | |
| CN108272455B (en) | Real-time oxygen consumption monitoring system for small animals with modularized water-seal anoxic bottles | |
| CN103105425B (en) | Water quality detection device and method | |
| CN110095424A (en) | A kind of four parameter online integrated apparatus of black and odorous water | |
| CN114184757B (en) | Method for measuring denitrification rate of suspended matters in water body | |
| CN206583897U (en) | A kind of chlorine residue detects flow cell | |
| CN115508043A (en) | Landslide accumulation body seepage hysteresis nature analogue test device | |
| CN204374083U (en) | A kind of online turbidimeter with flow control | |
| CN108514417B (en) | Respiration oxygen consumption real-time monitoring device with coiling oxygen consumption monitoring groove | |
| CN108514416B (en) | Respiration oxygen consumption real-time monitoring device with upper drainage structure | |
| CN201156034Y (en) | Water quality measuring instrument | |
| CN203720167U (en) | Intelligent water quality analyzer | |
| CN207816638U (en) | Water quality monitoring sampling system | |
| CN216350365U (en) | Algae detection inlet and outlet sample mechanism | |
| RU2798692C1 (en) | Stationary flow chamber for sampling gases at the water-atmosphere boundary | |
| CN110320091B (en) | Device for preparing saturated dissolved oxygen water | |
| KR101784377B1 (en) | Microorganism-reactor for toxicity measuring apparatus and toxicity measuring apparatus using thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150916 Termination date: 20161220 |