CN101585722A - Method for preparing composite biological fertilizer by combined solid fermentation of double bacterium - Google Patents
Method for preparing composite biological fertilizer by combined solid fermentation of double bacterium Download PDFInfo
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- CN101585722A CN101585722A CNA200910117168XA CN200910117168A CN101585722A CN 101585722 A CN101585722 A CN 101585722A CN A200910117168X A CNA200910117168X A CN A200910117168XA CN 200910117168 A CN200910117168 A CN 200910117168A CN 101585722 A CN101585722 A CN 101585722A
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Abstract
The invention relates to a method for preparing a composite biological fertilizer from tremellose bacillus and gigantochromoblast, which comprises: culturing and growing secondary tremellose bacillus strain and secondary gigantochromoblast strain; then, (1) stirring the solid culture medium uniformly, and placing into a disinfected solid fermentation pool, wherein, the weight percentage of the components are: wheat bran 15-25%, straw 25-36%, fly ash 11-19%, bean cake powder 3-9%, CaCO3 0.3-0.9%, turf 7.5-14%, K2HPO4 0.1-0.6%, sucrose 1-5%, and fertilizer soil 9-15%; (2) inoculating the two secondary strains into the solid fermentation pool according to that: the inoculation amount is 20% of the total weight, while the amount of the secondary tremellose bacillus strain is twice of the secondary gigantochromoblast strain; (3) adding water into the solid fermentation pool to keep the humidity of the ferment material at 50%, sealing and controlling the temperature in the solid fermentation pool, and fermenting and growing for 48-54 hours to obtain the finished product. The invention adopts combined solid fermentation of double bacterium, accelerates the strain breeding speed, shortens the fermentation period, reduces production cost, and improves the blastema formation ratio of the product.
Description
Technical field
The present invention relates to the microbial fertilizer production technology, be specifically related to utilize gel-shaped bacillus and Bacillus megatherium to prepare the method for composite biological fertilizer.
Background technology
In the prior art, after microbial fertilizer normally adopts the independent liquid fermenting of single culture, stir with solid adhesion again, make the microbial fertilizer of single performance.In recent years, the report of exploitation composite fungus agent is arranged also, but after the fermentation manufacturing technique that is adopted all is the independent liquid fermenting of each bacterial classification basically, stir with the solid adhesion mixed together again and form.For example, people such as Tang Xinyun have reported relevant composite microbiological fertilizer some problems (" biology magazine ", 18 the 1st phases of volume of 2000 year February) in production application in " problem in microbial fertilizer and the production application thereof " literary composition.People such as Cui Zhengzhong have also reported similar content in " noting problem in microbial fertilizer production, the application " (" northern gardening ", the 1st phase of calendar year 2001) literary composition.In general, the production of present applied microbial fertilizer mixes with solid adhesion with the physical agitation mode after just buying strain fermentation, not only the strain fermentation cycle is long, the production cost height, and owing to exist random composite situation to cause the product performance instability, make brood cell's rate of formation of product on the low side, difficult quality guarantee.
Summary of the invention
The objective of the invention is to,, provide a kind of gel-shaped bacillus and Bacillus megatherium of utilizing to carry out the method that combined solid fermentation prepares composite biological fertilizer at the deficiencies in the prior art.
Technical scheme of the present invention is as follows:
The method of utilizing combined solid fermentation of double bacterium to prepare composite biological fertilizer of the present invention, comprise and utilize shaking table shake-flask culture gel-shaped bacillus first class inoculum and Bacillus megatherium first class inoculum earlier, again these two kinds of first class inoculum are inoculated into seeding tank respectively, make it be grown to secondary gel-shaped bacillus kind and secondary Bacillus megatherium bacterial classification separately, it is characterized in that, operation steps is then carried out conventional sterilising treatment for (1) stirs solid medium to be placed in the solid fermentation pond, naturally cooling is stand-by, wherein the weight percentage of each component of solid medium is: wheat bran 15-25%, stalk 25-36%, flyash 11-19%, soybean cake powder 3-9%, CaCO
30.3-0.9%, careless carbon 7.5-14%, K
2HPO
40.1-0.6%, sucrose 1-5%, loam 9-15%; (2) secondary gel-shaped bacillus seed and secondary Bacillus megatherium bacterial classification are inoculated in the above-mentioned solid fermentation pond, turn and make solid medium and the sub-thorough mixing of second class inoculum, the inoculum size of described two kinds of second class inoculum accounts for 20% of solid medium and the sub-gross weight of second class inoculum, and wherein the inoculum size of secondary gel-shaped bacillus seed is 2 times of the sub-inoculum size of secondary Bacillus megatherium bacterial classification; (3) add water and make the humidity of fermentation materials remain on 50% in the solid fermentation pond, turn even back sealing solid fermentation vat and control temperature in the solid fermentation pond, the fermentation growth made finished product in 48-54 hour; Temperature in the described control solid fermentation pond is meant, make its 30-40 hour with the interior 28-35 of remaining on ℃, ventilation 0.7m
3/ min; After this growth latter temperature is promoted to 40 ℃, ventilation 0.3m
3/ min.At last above-mentioned finished product is carried out analyzing and testing, and the weighing and bagging warehouse-in.
In above-mentioned preparation process, described solid medium is stirred to be placed on carry out conventional sterilising treatment in the solid fermentation pond, be about to its sealing after, be heated to 121 ℃ of insulations 2 hours; Stalk in the described solid medium component is for pulverizing back 20 order powders, and all the other solid medium components also are Powdered.
The present invention utilizes combined solid fermentation of double bacterium to prepare the method for composite biological fertilizer, owing to adopted two bacterium combined solid fermented, can guarantee that gel-shaped bacillus and Bacillus megatherium carry out combined solid fermentation, compare with existing physical agitation mode and have tangible technical superiority.The first, accelerated culture propagation speed, shortened fermentation period, make the strain fermentation cycle shorten to 48-54 hour, it is long easily by the probability of living contaminants both to have reduced the growth phase, has reduced production cost again.And the fermentation period of similar bacterial classification generally needs 72-100 hour.The second, by the optimum combination of culture medium prescription, and control best fermentation parameter, make brood cell's rate of formation height of product.According to detection, gel-shaped bacillus and brood cell's rate of formation of combined solid fermented growth platform phase of Bacillus megatherium bacterial classification are up to 90%, and similar bacterial classification only is 50%, the highlyest are no more than 70%.
Be further described below by embodiment.
Embodiment 1 preparation secondary gel-shaped bacillus seed
1. by shaking table shake-flask culture gel-shaped bacillus (bacterial classification is bought from Beijing DSMZ of institute of microbiology of the Chinese Academy of Sciences) first order seed, 26-30 ℃ of shaking table temperature, shaking speed 180-200 rev/min, pH value 7.7-7.9, growth cycle 14-15 hour.
In the one-level culture medium prescription, the weight percent content of each component is: starch 84%, and ammonium sulfate 0.85, NaCL 15%, sucrose 0.15%.
2. place secondary seed medium and carry out sterilising treatment in seeding tank, seeding tank liquid sterilising temp is 121 ℃, and soaking time is 30 minutes, and seed liquor sterilization back pH value is 7.7-7.9; Then first order seed is inoculated in the seeding tank, makes the interior temperature of seeding tank be controlled at 28-30 ℃, tank pressure is 0.5kg/cm
2, 180 rev/mins of seeding tank rotating speeds, ventilation 10m
3/ hr, 12 hours production cycles.
In the secondary medium prescription, the weight percent content of each component is: starch 84%, and ammonium sulfate 0.85, NaCL 15%, sucrose 0.15%, soybean cake powder 0.3%.
Described preparation process of present embodiment and material therefor and prior art are roughly the same, and wherein each component in one-level culture medium prescription and the secondary medium prescription is buied by market.(Chemicals of employing are technical grade)
Embodiment 2 preparation secondary Bacillus megatherium seeds
1. by shaking table shake-flask culture Bacillus megatherium (bacterial classification is bought from Beijing DSMZ of institute of microbiology of the Chinese Academy of Sciences) first order seed, 30 ℃ of shaking table temperature, shaking speed 180-200 rev/min, pH value 7.5-7.8, growth cycle 14 hours.
2. place secondary seed medium and carry out sterilising treatment in seeding tank, seeding tank liquid sterilising temp is 121 ℃, and soaking time is 30 minutes, and seed liquor sterilization back pH value is 7.5-7.8; Then first order seed is inoculated in the seeding tank, inoculum size be first order seed account for total amount (first order seed+secondary seed medium) 10%, make that temperature is controlled at 30 ℃ ± 1 in the seeding tank, tank pressure is 0.4-0.6kg/cm
2, 180 rev/mins of seeding tank rotating speeds, ventilation 7m
3/ hr, 12 hours production cycles.
In the prescription of I and II substratum, the weight percent content of each component is: peptone 30%, and beef extractive substance 9%, NaCl 15%, agar 45%, light calcium carbonate 0.7%, FeCl
30.3%.
Described preparation process of present embodiment and material therefor are same as the prior art.Wherein each component in one-level culture medium prescription and the secondary medium prescription is buied by market.(Chemicals of employing are technical grade)
Embodiment 3
Utilize the combined solid fermented explained hereafter composite biological fertilizer of gel-shaped bacillus and Bacillus megatherium
(1) take by weighing each component of solid medium by the prescription requirement, count 100 kilograms, wherein wheat bran is 23 kilograms, 30 kilograms of stalks, 15 kilograms in flyash, 6 kilograms of soybean cake powder, CaCO
3(unslaked lime) 0.7 kilogram, 10 kilograms in careless carbon, K
2HPO
40.3 kilogram, 3 kilograms of sucrose, 12 kilograms of loam, described stalk is for pulverizing back 20 orders, and all the other each components are Powdered;
More than each component by market purchasing, K
2HPO
4Be powder-like product.
(2) each component is mixed and stir, send into the solid fermentation pond and sterilize, after the sealing of solid fermentation pond, be heated to 121 ℃ and be incubated 2 hours, naturally cooling is stand-by;
(3) secondary gel-shaped bacillus seed, secondary Bacillus megatherium bacterial classification in embodiment 1 and embodiment 2 seeding tanks are inoculated into respectively in the solid fermentation pond, add water then, turn with automatic tripper and to make solid medium and the sub-thorough mixing of bacterial classification, wherein the consumption of secondary gel-shaped bacillus seed is 16.7 kilograms, the consumption of secondary Bacillus megatherium bacterial classification is 8.3 kilograms, and amount of water is 18.75 kilograms;
(4) sealing solid fermentation vat, and control its interior temperature at 28-35 ℃, ventilation 0.7m
3/ min makes in the pond temperature increase to 40 ℃, ventilation 0.3m after 40 hours
3/ min, 50 hours must finished product.
Below be detection data to the present embodiment product:
No mould, tasteless (or have little ammonia flavor) held agglomeratingly, and that touches promptly looses, water content 25-35%, gel-shaped bacillus viable count 〉=1.1 hundred million/gram, huge brood cell's bar viable count 〉=1.6 hundred million/gram, bacterium number≤10% of mixing.Detect data declaration, this product meets the requirement of relevant composite biological fertilizer.
Embodiment 4
(1) take by weighing each component of solid medium by the prescription requirement, count 180 kilograms, wherein wheat bran is 41.4 kilograms, 54 kilograms of stalks, 27 kilograms in flyash, 10.8 kilograms of soybean cake powder, CaCO
31.26 kilogram, 18 kilograms in careless carbon, K
2HPO
40.54 kilogram, 5.4 kilograms of sucrose, 21.6 kilograms of loam, described stalk is for pulverizing back 20 orders;
(2) each component is mixed and stir, send into the solid fermentation pond and sterilize, after the sealing of solid fermentation pond, be heated to 121 ℃ and be incubated 2 hours, naturally cooling is stand-by;
(3) then secondary gel-shaped bacillus seed, secondary Bacillus megatherium bacterial classification in embodiment 1 and embodiment 2 seeding tanks are inoculated into respectively in the solid fermentation pond, add entry simultaneously, turn with automatic tripper and to make solid medium and the sub-thorough mixing of bacterial classification, wherein the consumption of secondary gel-shaped bacillus seed is 30 kilograms, the consumption of secondary Bacillus megatherium bacterial classification is 15 kilograms, and amount of water is 33.75 kilograms;
(4), the sealing solid fermentation vat keeps humidity 50%, and controls its interior temperature at 28-35 ℃, ventilation 0.7m
3/ min makes in the pond temperature increase to 40 ℃, ventilation 0.3m after 35 hours
3/ min, 52 hours must finished product.
Claims (1)
1. method of utilizing combined solid fermentation of double bacterium to prepare composite biological fertilizer, comprise and utilize shaking table shake-flask culture gel-shaped bacillus first class inoculum and Bacillus megatherium first class inoculum earlier, again these two kinds of first class inoculum are inoculated into seeding tank respectively, make it be grown to secondary gel-shaped bacillus kind and secondary Bacillus megatherium bacterial classification separately, it is characterized in that, operation steps is then carried out conventional sterilising treatment for (1) stirs solid medium to be placed in the solid fermentation pond, naturally cooling is stand-by, wherein the weight percentage of each component of solid medium is: wheat bran 15-25%, stalk powder 25-36%, flyash 11-19%, soybean cake powder 3-9%, CaCO
30.3-0.9%, careless carbon dust 7.5-14%, K
2HPO
40.1-0.6%, sucrose 1-5%, loam 9-15%; (2) secondary gel-shaped bacillus seed and secondary Bacillus megatherium bacterial classification are inoculated in the above-mentioned solid fermentation pond, turn and make solid medium and the sub-thorough mixing of second class inoculum, the inoculum size of described two kinds of second class inoculum accounts for 20% of solid medium and the sub-gross weight of second class inoculum, and wherein the inoculum size of secondary gel-shaped bacillus seed is 2 times of the sub-inoculum size of secondary Bacillus megatherium bacterial classification; (3) add water and make the humidity of fermentation materials remain on 50% in the solid fermentation pond, turn even back sealing solid fermentation vat and control temperature in the solid fermentation pond, the fermentation growth made finished product in 48-54 hour; Temperature in the described control solid fermentation pond is meant, make its 3040 hours with the interior 28-35 of remaining on ℃, ventilation 0.7m
3/ min; After this growth latter temperature is promoted to 40 ℃, ventilation 0.3m
3/ min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910117168XA CN101585722B (en) | 2009-06-26 | 2009-06-26 | Method for preparing composite biological fertilizer by combined solid fermentation of double bacterium |
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|---|---|---|---|
| CN200910117168XA CN101585722B (en) | 2009-06-26 | 2009-06-26 | Method for preparing composite biological fertilizer by combined solid fermentation of double bacterium |
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| CN101585722B CN101585722B (en) | 2011-05-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342371A (en) * | 2011-09-01 | 2012-02-08 | 安徽金农惠民生物技术有限公司 | Process for preparing compound feed additive from bacillus subtilis natto and bacillus licheniformis |
| CN109438004A (en) * | 2018-11-06 | 2019-03-08 | 山东志华农业开发有限公司 | A kind of method of straw-returning getting fat in greenhouse |
-
2009
- 2009-06-26 CN CN200910117168XA patent/CN101585722B/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342371A (en) * | 2011-09-01 | 2012-02-08 | 安徽金农惠民生物技术有限公司 | Process for preparing compound feed additive from bacillus subtilis natto and bacillus licheniformis |
| CN102342371B (en) * | 2011-09-01 | 2013-01-23 | 安徽金农惠民生物技术有限公司 | Process for preparing compound feed additive from bacillus subtilis natto and bacillus licheniformis |
| CN109438004A (en) * | 2018-11-06 | 2019-03-08 | 山东志华农业开发有限公司 | A kind of method of straw-returning getting fat in greenhouse |
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| CN101585722B (en) | 2011-05-04 |
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Owner name: ANHUI JINNONG HUIMIN BIOLOGY TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ANHUI JINNONG BIOLOGY TECHNOLOGY CO., LTD. Effective date: 20110930 |
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Effective date of registration: 20110930 Address after: 230088 No. 33 Hongfeng Road, Hefei hi tech Development Zone, Anhui, China Patentee after: Anhui Jinnong Huimin Biology Technology Co.,Ltd. Address before: 230088 No. 33 Hongfeng Road, Hefei hi tech Industrial Development Zone, Anhui, China Patentee before: Anhui Jinnong Biology Technology Co., Ltd. |
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