CN101401524A - Cutting propagation method for beach plum epicormic branch - Google Patents

Abstract

The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a cuttage seedling raising method for twigs of prunus maritime. The method solves the problems of cuttage seedlings of prunus maritime of rotten calluses of roots, low rooting rate, low rate of survival, week seedlings and susceptibility to water loss and withering of prunus maritime and strengthens the growth and rejuvenation of the roots, stems and leaves of the seedlings during cuttage through hardening seedlings, improves the rate of survival in wild field planting and lays firm good function fur special planting seedlings of pruns maritime.

Description

Cutting propagation method for beach plum epicormic branch

One, technical field

This invention belongs to the plant asexual multiplication technology field, is specifically related to a kind of cutting propagation method for beach plum epicormic branch.

Two, background technology

Shore plum (Prunus maritima) is a kind of desert shrub fruit tree, originates in the U.S., Atlantic bank, from the Newfoundland to North Carolina Na Zhou distribution is arranged all.It can adapt to existence in arid (comprising very low soil moisture content), barren, strong wind, sand, unstable matrix and highly-saline environment, have sweet good to eat fruit, has the characteristics of beautifying the environment and recovering husky living vegetation concurrently.The shore plum will be a pioneer that stands in the breach in the development of beach and salt tolerant orchard, other solonchak zone industry.Domestic successful introduction, but the bottleneck of brood body supply has greatly limited its popularization cultivation in China.Make remarkable progress aspect breeding at cell engineering Seedling with the research team headed by the Nanjing University, but also have problems such as cost is too high, the survival rate of the open-air field planting of tissue cultivating seedling is not high.Therefore carry out the road that propagation in scale is undoubtedly another light by low-cost, cuttage technique easy and simple to handle.But the shore plum typical problems such as the root callus rots, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers often occur in the cuttage process.Therefore, the selection of cutting medium, the selection of plugged ear and cutting cuts, the control, the method for hardening etc. of ecological factors such as temperature, humidity and illumination after the selection of hormone and using method, the cuttage, raising and the healthy and strong growth of seedling body to the callus healthy growth of shore plum seedling, rooting rate, survival rate are most important, and optimizing and combining of correlative factor is the cuttage technique key of success.

Three, summary of the invention

The problem that the present invention need solve is: overcome the problem that shore plum cuttage shoot root callus rots, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers and transplanting survival rate is low.

Concrete technical scheme of the present invention is: the present invention is directed to the problem that callus is perishable in the feature of shore plum and biological property and the cuttage process, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers and growing-seedling period is long, invented the shore plum epicormic branch cuttage method of " choosing branch → lopping with dosing → immersion and seedbed arrangement → cuttage → control water, control light, temperature control → spraying liquid and nutrient solution → Cheng Miao → transplanting ".Difficult problems such as callus is perishable in the plum epicormic branch cuttage process of shore, have been solved, perfect moisture and nutrition (particularly nitrogen, P elements) supply conditions, strengthened growing and rejuvenation of root in the plum cuttage seeding process of shore, stem, leaf, significantly improved its survival rate, promoted laying a solid foundation for the cultivation of characteristic woods fruit shore plum seedling.

Concrete technical scheme is as follows:

1. select the Best Times and the points for attention of branch.

2. lopping and respective handling method

3. the determining of the screening of hormone kind, concentration, optimum organization and processing time

4. slotting machine arrangement

5. cuttage and insert back management (water control, temperature control, light-operated etc.)

6. bush mycorrhiza agent hardening and transplanting method

The present invention compared with prior art, its beneficial effect is:

Cuttage and seedling culture is easy and simple to handle, saves branch, and breeding amount is big, and cost is low and can keep parent's proterties.The shore plum is in the cuttage process, and the normal typical problem that occurs is: cuttage shoot root callus rots, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers.Use the present invention to address the above problem effectively, significantly improve survival rate, and the strong stress resistance of seedling.Innovation part of the present invention mainly contains following points: the 1. 2. 3. 3. bush mycorrhiza agent hardening of management of the factors such as cuttage Hou Shui, temperature, light of the hormone kind of optimum organization and concentration of the method for the time of choosing branch and lopping.Use the survival rate of shore of the present invention plum cuttage seeding can reach more than 84%, open-air transplanting survival rate can reach more than 90%, really realizes the asexual fast numerous advantage of shore plum seedling, promotes cultivation for its large tracts of land reliable guarantee is provided.

Four, embodiment

1. select branch: gather mid-May, and about 20～30cm band leaf will be put into the container that adds 5～10cm deep water of preparation in advance at any time after adopting, and in the clip immersion water, a large amount of branch collections take long adopting when the back branch is grown and are exposed to the sun, and deposit in the place, cool place;

2. lopping: the branch of adopting back from the field is cut the plugged ear that is cut into long 8～10cm immediately, 2 blades are stayed at the plugged ear top, the top tip can suitably stay longer, 10-12cm, top tip blade is because less, can stay 2～3 leaves, the scion that shears is an a bundle with the 50-100 branch, and the carbendazim soup that immerses 1000ppm is handled;

3. dosing and processing: indolebutyric acid and methyl liquid were mixed in by volume 1: 1, and separately final concentration is 1000ppm in the mixed liquor.The base portion of transplanting a cutting adopts fast libation at an ancient wedding ceremony method, uses above-mentioned mixed liquor to handle 10s, again with to be inserted behind the active carbon on the otch libation at an ancient wedding ceremony;

4. seedbed arrangement: the seedbed is arranged in the green house, the thick cobblestone of slotting machine bottom shop 20cm, and the thick thin river sand of 20cm is spread on top, and automatic sprayer is installed on the slotting machine;

5. cuttage and the management of slotting back: the cutting after soup is handled, slotting machine is inserted in the cutting of active carbon immediately on the otch libation at an ancient wedding ceremony, slotting machine humidity is 30%～40%, maintain before air humidity is taken root more than 80%, automatic sprayer is adjusted into early, in, evening, three shelves were controlled water spraying time automatically, spray water 1 time every 15min noon, sooner or later 45min sprays water 1 time at interval, and the water spray duration is as the criterion with wetting blade face, general 10s, with the illumination of sunshade network control system, insert in the 3d of back and see astigmatism, see direct light, shading at noon after 2 weeks gradually, whole day is seen light behind the 30d, the slotting machine temperature is than the high 2-3 of air themperature ℃, and maintains 22-25 ℃, if air themperature can be sprayed when being higher than 25 ℃ or be aeration-cooling, insert every 1 carbendazim 1000ppm soup of 3d spray in back or thiophanate methyl 800ppm soup, hocket, behind the cutting growth 45d, in water spray, add the spray nutrient solution, the nutrient composition proportioning sees Table 1, and nutrient solution and spray carbendazim soup carry out simultaneously, every 3d spray 1 time.

6. hardening and transplanting: can hardening after 60 days, during hardening, the inoculation commercially available Glomus mosseae (Nicol.﹠amp in Beijing City Agriculture and Forestry Institute; Gerd.) microbial inoculum, hardening matrix are the peat composed of rotten mosses of 2 parts of volumes and the mellow soil mixture of 1 part of volume, every basin inoculation 20g, the basin of inoculation basin directly is 13cm, the high 13cm of basin, and temperature, illumination condition during hardening are the same, stop-spraying carbendazim soup, each week spray one time of nutrition liquid, the control water yield, be as the criterion with not dehydration of blade, reduce the water yield gradually, water spray is spaced apart 120min sooner or later, noon, water spraying time was 5s, be spaced apart 30min, can open-air field planting after 90 days, preceding 1 week of field planting puts the seedling basin in the open.

Table 1 nutrient composition proportioning

	Composition	Dosage (g/L)		Composition	Dosage (g/L)
						1	KH2PO 4	0.14	7	Ironic citrate	0.02
2	KNO 3	0.51	8	ZnSO 4·7H 2O	0.22
						3	Ca(NO 3) 2	0.82	9	CuSO 4·5H 2O	0.08
4	MgSO 4	0.49	10	H 2MO 4·H 2O	0.02
						5	H 3BO 3	2.86
6	MnCl 2·4H 2O	1.81

Claims (1)

1, a kind of cutting propagation method for beach plum epicormic branch is characterized in that being made of following steps:

1. select branch: gather mid-May, and about 20～30cm band leaf will be put into the container that adds 5～10cm deep water of preparation in advance at any time after adopting, and in the clip immersion water, a large amount of branch collections take long adopting when the back branch is grown and are exposed to the sun, and deposit in the place, cool place;

2. lopping: the branch of adopting back from the field is cut the plugged ear that is cut into long 8～10cm immediately, 2 blades are stayed at the plugged ear top, the top tip can suitably stay longer, 10-12cm, top tip blade is because less, can stay 2～3 leaves, the scion that shears is an a bundle with the 50-100 branch, and the carbendazim soup that immerses 1000ppm is handled;

3. dosing and processing: indolebutyric acid and methyl liquid were mixed in by volume 1: 1, and separately final concentration is 1000ppm in the mixed liquor.The base portion of transplanting a cutting adopts fast libation at an ancient wedding ceremony method, uses above-mentioned mixed liquor to handle 10s, again with to be inserted behind the active carbon on the otch libation at an ancient wedding ceremony;

4. seedbed arrangement: the seedbed is arranged in the green house, the thick cobblestone of slotting machine bottom shop 20cm, and the thick thin river sand of 20cm is spread on top, and automatic sprayer is installed on the slotting machine;

5. cuttage and the management of slotting back: the cutting after soup is handled, slotting machine is inserted in the cutting of active carbon immediately on the otch libation at an ancient wedding ceremony, slotting machine humidity is 30%～40%, maintain more than 80% before air humidity is taken root, automatic sprayer is adjusted into early, in, evening, three shelves were controlled water spraying time automatically, and spray water 1 time every 15min noon, sooner or later 45min sprays water 1 time at interval, the water spray duration is as the criterion with wetting blade face, and general 10s is with the illumination of sunshade network control system, insert in the 3d of back and see astigmatism, see direct light after 2 weeks gradually, the shading at noon, whole day is seen light behind the 30d, the slotting machine temperature is than the high 2-3 of air themperature ℃, and maintain 22-25 ℃, if air themperature can be sprayed when being higher than 25 ℃ or be aeration-cooling, insert every 1 carbendazim 1000ppm soup of 3d spray in back or thiophanate methyl 800ppm soup, hocket, transplant a cutting behind the growth 45d, in water spray, add the spray nutrient solution, nutrient solution and spray carbendazim soup carry out simultaneously, every 3d spray 1 time;

6. hardening and transplanting: can hardening after 60 days, during hardening, the inoculation commercially available Glomus mosseae (Nicol.﹠amp in Beijing City Agriculture and Forestry Institute; Gerd.) microbial inoculum, hardening matrix are the peat composed of rotten mosses of 2 parts of volumes and the mellow soil mixture of 1 part of volume, every basin inoculation 20g, the basin of inoculation basin directly is 13cm, the high 13cm of basin, and temperature, illumination condition during hardening are the same, stop-spraying carbendazim soup, each week spray one time of nutrition liquid, the control water yield, be as the criterion with not dehydration of blade, reduce the water yield gradually, water spray is spaced apart 120min sooner or later, noon, water spraying time was 5s, be spaced apart 30min, can open-air field planting after 90 days, preceding 1 week of field planting puts the seedling basin in the open.