CN101278977A - Method for extracting main active components of mulberry leaf and application of its extract - Google Patents
Method for extracting main active components of mulberry leaf and application of its extract Download PDFInfo
- Publication number
- CN101278977A CN101278977A CNA200810027097XA CN200810027097A CN101278977A CN 101278977 A CN101278977 A CN 101278977A CN A200810027097X A CNA200810027097X A CN A200810027097XA CN 200810027097 A CN200810027097 A CN 200810027097A CN 101278977 A CN101278977 A CN 101278977A
- Authority
- CN
- China
- Prior art keywords
- extract
- resin
- folium mori
- ethanol
- methanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a method for extracting the main active components of mulberry leaves and the application of extract, belonging to phytochemistry, Chinese medicine modernization and the field of preventing and treating diabetes mellitus. The invention is characterized in that only one resin is required and three kinds of the active components of the mulberry leaves, which are alkaloids, flavonoids and polysaccharide, are completely extracted by one extraction process. Therefore, the extracting method has simple technique and low cost. The preparation method comprises processes of extraction, adsorption, elution and drying. The main active components of the extract prepared by the method are the alkaloids, the flavonoids and the polysaccharide extracted from the mulberry leaves. The extract can be used as granule without excipient added or be used as the medicine or functional food with one or a plurality of edible excipient added, such as starch, microcrystalline cellulose, dextrin, sucrose, lactose, Vitamin C, citric acid and sodium sulfite, for preventing and treating diabetes mellitus and the complication of diabetes mellitus and obesity and treating viral col.
Description
Technical field
The present invention relates to the method that a kind of main active components of mulberry leaf extracts, and extract belongs to the phytochemistry and the modernization of Chinese medicine, diabetes control field preventing and treating diabetes and complication thereof, obesity and treating application aspect the viral influenza.
Background technology
Diabetes be a kind of be cardinal symptom group's gradual endocrinopathy with the carbohydrate metabolism disturbance, basic pathology physiology is reason such as insulin secretion function relative deficiency or absolute deficiency or Insulin receptor INSR dysfunction in the body, cause metabolism disorder, comprise sugar, protein, fat, water and electrolyte etc.Generally show as hyperglycemia, glycosuria and hyperlipemia, the ketone acidemia can take place in serious case, even loop attenuation and stupor and threat to life.Diabetes are divided into four classes, and wherein maximum type ii diabetes accounts for the 90-95% of diabetes sum.
Activity less, obesity and aging cause type ii diabetes to increase fast.It is reported that China's diabetics was 2.5%, 1996 year 3.21%, 1998 year 3.8% in 1993, rise to now more than 5% that growth rate is accelerated year by year.It is estimated that China's diabetics has reached 5,000 ten thousand people at present, and increase with the speed that increases 1,000,000 people every year newly.
Folium Mori are the Chinese medicine of the medicine-food two-purpose of Ministry of Public Health announcement, in morning among the people application are arranged, and being usually used in treating diabetes with other Chinese prescriptions is diabetes.Modern study has been illustrated the part mechanism of Folium Mori treatment diabetes.Polyhydroxylated alkaloid in the Folium Mori of at first finding by Japanese scholar have with saccharide like structure, but the activity of the disaccharide-hydrolysing enzymes-alpha-glucosidase in can the retroactive inhibition small intestinal slow down glucose and enter blood, reduce post-prandial glycemia.This Alkaloid has and the similar structure of the acarbose of present clinical use and the mechanism of action.Simultaneously, studies have shown that alpha-glucosidase inhibitor also has obesity, antiviral therapeutical effect.In addition, there are lot of documents report flavone, polysaccharide and glycoprotein also to have hypoglycemic activity.Comprehensive existing achievement in research, the main active that proves Folium Mori is alkaloid, flavone, polysaccharide.
Publication number is that the Chinese patent of CN1338275A discloses " Chinese medicine extract, Preparation Method And The Use with alpha-glucosidase inhibitor activity ", and this patent has related to and prepared total alkaloids and be used for diabetes from Chinese medicine Folium Mori, Ramulus Mori, Cortex Mori and Fructus Mori.Publication number be CN1250246C patent disclosure " medical composition and its use " with alpha-glucoside inhibiting activity, this patent has related to the application of compositions in treatment diabetes, hyperlipidemia, antioxidation and antiaging agent of the total alkaloids for preparing in Chinese medicine Folium Mori, Ramulus Mori, Cortex Mori and the Fructus Mori and flavone compound Quercetin, catechin and the tea polyphenols that adds.Publication number be CN1850166A patent disclosure Folium Mori effective ingredient polysaccharides of Folium Mori and Folium Mori alkaloid preparation of compositions method, application number be CN1981814A patent disclosure " a kind of Folium Mori extract and in application and the preparation method of preparation in the diabetes medicament " related to and from Folium Mori, extract total flavones and total alkaloids and mix the back preparation with certain proportion and become and can prevent and treat the preparation of controlling diabetes and complication thereof.Publication number is that CN1742803A discloses a kind of mode that adopts macroporous adsorbent resin and cation exchange resin to use continuously and extracts flavone and alkaloid.Said method is the representational extracting method of Folium Mori anti-diabetic composition in recent years.But there is certain deficiency in these methods: perhaps the utilization of Folium Mori extracts active ingredients is incomplete, and perhaps technical process is more loaded down with trivial details, can not separate all main effective ingredient by primary sorption.
At above-mentioned deficiency, we have invented a kind of efficient, economic extracting method of Folium Mori, can pass through a leaching process, being about to main active component alkaloid, flavone and the polysaccharide of Folium Mori all extracts, and only need adopt a kind of resin, and technology is simple, and cost is low.
Summary of the invention
The method that the present invention taked is to utilize the large hole cation exchanger resin with ion exchange and adsorption function to adsorb effective ingredient alkaloid and flavone as adsorbent, can these two kinds of active component of disposable absorption, obtain polysaccharide by methanol or ethanol precipitation simultaneously.The present invention only need adopt a kind of resin, by a leaching process, uses the effect of two kinds of resins before just can reaching, and three class main active alkaloids, flavone and the polysaccharide of Folium Mori all extracted.Regeneration of resin is also more time saving and energy saving.More do not need to adopt other complexity, the higher technology of cost.Whole extraction and separation process does not use other inflammable and explosive organic solvent except methanol or ethanol, non-environmental-pollution, and safety non-toxic is beneficial to scale and extracts production.
For achieving the above object, the present invention has adopted following technical scheme:
1) extract: with Folium Mori is raw material, water or aqueous ethanol or methanol heating extraction;
2) Chun recovery: during with methanol or ethanol-water extraction, reclaim under reduced pressure methanol or ethanol obtain aqueous solution;
3) absorption: aqueous extract is by large porous strong acid type or weak-type absorption and exchanger resin, and active component is attracted on the resin.
4) eluting: make eluant with methanol or ethanol/water solution earlier, eluent concentrates and obtains flavones ingredient; Use the ammonia eluting then, concentrate and obtain the alkaloids composition;
5) with methanol or ethanol precipitation, precipitate was polysaccharide after the resin of step 3) concentrated by liquid.
6), promptly obtain the effective ingredient product of Folium Mori with above-mentioned flavone, alkaloid and polysaccharide concentrated solution mixing after drying.
Extract the ethanol of usefulness or the concentration of methanol, its scope is between 5-95%, and consumption is 5-15 times.Heating-up temperature is between 30-100 ℃.
Used macroporous absorption and exchanger resin, the main strongly acidic cation-exchange with absorption and function of exchange that adopts as the D72 resin, also can use the weak-type macroporous adsorbent resin.Consumption is 0.5-5 a times of raw material weight.
The ethanol that eluting is used or the concentration of methanol, its scope are between 10-95%, and consumption is a 1-10 column volume.The concentration of ammonia is between 0.5%-15%, and consumption is a 1-10 column volume.
Drying means is vacuum drying, spray drying or lyophilization.
By the Folium Mori extract that method for preparing forms, main active is formed for the alkaloid, flavone and the polysaccharide that directly extract from Folium Mori, but not the analogous components that adds in addition.This Folium Mori extract does not add adjuvant promptly can directly be used as electuary; Perhaps add edible adjuvant, as in starch, microcrystalline Cellulose, dextrin, sucrose, lactose, vitamin C, citric acid and the sodium sulfite any one or a few, be prepared into dosage forms such as tablet, capsule or oral liquid, be used to prevent and treat diabetes and complication thereof, prevent and treat obesity and treatment viral influenza as medicine or functional food.
The invention has the advantages that extraction and preparation technique is simple, main active can once all obtain, safety, and easy operating does not need special installations such as High Temperature High Pressure, and cost is low.
The specific embodiment
Describe technical scheme of the present invention in detail below by embodiment, but be not limited to cited example.
Embodiment 1
Folium Mori 10kg shreds, and extracts 2 times each 50L in 30 minutes with water boil.Extracting solution leaves standstill cool to room temperature, gets the supernatant by D72 resin column (column volume 10L), and the 20L ethanol elution is used in 10L washing back, uses ammonia/ethanol water (containing ethanol 70%) solution 30L eluting of 1% then.After the ammonia ethanol elution was recovered to no ammonia, the 20L ethanol elution before adding was recycled to the 3L volume, obtains fluid extract.
Resin boils off most of water to 3L by liquid, adds the 7L ethanol precipitation.The centrifugal supernatant of removing mixes with top fluid extract, and vacuum drying is pulverized, and obtains yellow powder 300g, yield 3%.
Get Folium Mori extract 100g, add starch 3000g, microcrystalline Cellulose 30g, with 50% alcohol granulation, tabletting gets tablet, and every 0.6g wherein contains extract 20mg, is equivalent to Folium Mori 0.7g.
Embodiment 2
Folium Mori 10kg shreds, and boils with 50% ethanol/water and extracts 2 times in 30 minutes, each 50L.After extracting solution concentrates and reclaims ethanol, add water 100L dilution, then by D72 resin column (column volume 10L).Handle according to embodiment 1 described mode, obtain yellow powder 400g, yield 4% is the Folium Mori extract of indication of the present invention.
The alpha-glucosidase of above-mentioned Folium Mori extract suppresses active test:
The alpha-glucosaccharase enzymatic solution is measured protein concentration then with the jejunum preparation of Wister rat.The Folium Mori extract water is mixed with the solution for standby of 0.05g/m1.Sample sets with jejunum homogenate 50 μ 1, sucrose 50 μ l, be equipped with sample product 50 μ l (final concentration is 5 μ g/ μ l), PBS 50 μ l.Positive control acarbose, final concentration are 0.5 μ g/ μ l.A blank group is set in addition, and glycosidase wherein is by high-temperature inactivation.Other compositions of criterion group are identical, just do not add sample or glucobay (acarbose).Above-mentioned every group of parallel work 7 pipes were hatched 25 minutes for ready back 37 ℃.Hatch and finish the Na that the back adds 0.1mol/L
2CO
3200 μ l stopped reactions.Every pipe 50 μ l that take a sample measure glucogenic amount by the method that the blood sugar test test kit provides.
Suppression ratio=((1-(glucobay (acarbose) group concentration of glucose-blank group concentration of glucose)/(criterion group concentration of glucose-blank group concentration of glucose)) * 100%
According to top formula, the alpha-glucosaccharase enzyme inhibition rate that calculates embodiment 1 and 2 extracts is respectively 90% and 88%.
Claims (6)
1, a kind of Folium Mori extracting method is characterized in that only needs adopt a kind of resin, by a leaching process, three class main active alkaloids, flavone and the polysaccharide of Folium Mori all can be extracted.
2, the method for a kind of whole extraction Folium Mori alkaloid, flavone and polysaccharide is as follows:
1) extract: with Folium Mori is raw material, water or aqueous ethanol or methanol heating extraction;
2) Chun recovery: during with methanol or ethanol-water extraction, reclaim under reduced pressure methanol or ethanol obtain aqueous solution;
3) absorption: aqueous extract is by large porous strong acid type or weak-type exchanger resin, and active component is attracted on the resin;
4) eluting: make eluant with methanol or ethanol/water solution earlier, eluent concentrates and obtains flavones ingredient; Use the ammonia eluting then, concentrate and obtain the alkaloids composition;
5) with methanol or ethanol precipitation, precipitation was polysaccharide after the resin of step 3) concentrated by liquid;
6), promptly obtain the effective ingredient product of Folium Mori with above-mentioned flavone, alkaloid and polysaccharide combination drying.
3, in the claim 2, the concentration range of ethanol or methanol is between 5-95% in the solution of extraction usefulness, and consumption is 5-15 times, and the heating extraction temperature is 30-100 ℃; The ethanol that eluting is used or the concentration of methanol, its scope are between 10-95%, and the content of ammonia is between 0.5%-15%.
4, used large porous strong acid type or weak-type exchanger resin in the claim 2, the main large porous strong acid cation exchange resin with absorption and function of exchange that adopts as the D72 resin, also can use the weak-type macroporous adsorbent resin.
5, a kind of extract of main active components of mulberry leaf, the method by claim 2, claim 3, claim 4 obtains, and it is characterized in that main active is made of the alkaloid that extracts from Folium Mori, flavone and polysaccharide.
6, the Folium Mori extract that obtains by claim 2, claim 3, claim 4, can directly use as electuary, also can add in edible adjuvant such as starch, microcrystalline Cellulose, dextrin, sucrose, lactose, vitamin C, citric acid and the sodium sulfite any one or a few, as preparation prevent and treat diabetes and complication thereof, control is fat and the medicine or the functional food of treatment viral influenza.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810027097XA CN101278977B (en) | 2008-03-31 | 2008-03-31 | Method for extracting main active components of mulberry leaf and application of its extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810027097XA CN101278977B (en) | 2008-03-31 | 2008-03-31 | Method for extracting main active components of mulberry leaf and application of its extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101278977A true CN101278977A (en) | 2008-10-08 |
| CN101278977B CN101278977B (en) | 2012-05-30 |
Family
ID=40011764
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810027097XA Expired - Fee Related CN101278977B (en) | 2008-03-31 | 2008-03-31 | Method for extracting main active components of mulberry leaf and application of its extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101278977B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869605A (en) * | 2010-07-02 | 2010-10-27 | 安徽济人药业有限公司 | Method for extracting and separating mulberry leaf flavone and alkaloid composite |
| US20110065752A1 (en) * | 2009-09-04 | 2011-03-17 | United Therapeutics Corporation | Methods of treating orthomyxoviral infections |
| CN102204985A (en) * | 2011-06-10 | 2011-10-05 | 广东省农业科学院蚕业与农产品加工研究所 | Compound lychee seed hpyerglycemic particles and preparation method thereof |
| CN101554406B (en) * | 2009-05-14 | 2012-08-08 | 浙江省农业科学院 | Extraction process and formulation of mulberry cortex hypoglycemic medicinal active material |
| CN104435746A (en) * | 2014-11-05 | 2015-03-25 | 贵州赤水国礼金钗石斛发展有限公司 | Method for simultaneously extracting polysaccharides, flavones and alkaloid from dendrobium stem |
| CN104940293A (en) * | 2015-06-15 | 2015-09-30 | 塔里木大学 | Method for extracting alkaloid, polysaccharide and flavone from mulberry leaves |
| CN107721909A (en) * | 2017-11-02 | 2018-02-23 | 厦门福美科技有限公司 | DNJ, flavones, the method and system of polysaccharide are continuously extracted from moraceae plants |
| CN107951927A (en) * | 2017-12-15 | 2018-04-24 | 佛山市米良仓科技有限公司 | The extracting method of active material in medicine mulberry leaf |
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
| CN108785388A (en) * | 2017-04-26 | 2018-11-13 | 奥维嘉生物科技（北京）有限公司 | A kind of composite formula and preparation method treating and preventing diabetes and its complication |
| CN109463399A (en) * | 2018-11-20 | 2019-03-15 | 安徽省应用技术研究院 | A kind of anti-mulberry-leaf extract and preparation method thereof to eliminate aphis |
| CN113509484A (en) * | 2021-08-12 | 2021-10-19 | 天津大学 | Application of mulberry leaf polysaccharide in preparation of weight-losing medicine or weight-losing health-care product |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387620B (en) * | 2013-08-08 | 2015-09-23 | 福建中医药大学 | Polysaccharide, total flavones and total alkaloids and preparation method thereof is prepared from Plumula Nelumbinis |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004217532A (en) * | 2003-01-10 | 2004-08-05 | Maruzen Pharmaceut Co Ltd | Mulberry leaf extract and method for producing the same, and anti-hyperglycemic composition and obesity-preventing composition |
| CN100361674C (en) * | 2005-08-11 | 2008-01-16 | 原爱红 | Chinese medicine extract with function of reducing blood-sugar and preparing method |
| CN1981814A (en) * | 2005-12-15 | 2007-06-20 | 天津中新药业集团股份有限公司 | Use and production for mulberry leaf in treatment of diabetes |
-
2008
- 2008-03-31 CN CN200810027097XA patent/CN101278977B/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101554406B (en) * | 2009-05-14 | 2012-08-08 | 浙江省农业科学院 | Extraction process and formulation of mulberry cortex hypoglycemic medicinal active material |
| US20110065752A1 (en) * | 2009-09-04 | 2011-03-17 | United Therapeutics Corporation | Methods of treating orthomyxoviral infections |
| CN101869605B (en) * | 2010-07-02 | 2012-05-02 | 安徽济人药业有限公司 | Method for extracting and separating mulberry leaf flavone and alkaloid composite |
| CN101869605A (en) * | 2010-07-02 | 2010-10-27 | 安徽济人药业有限公司 | Method for extracting and separating mulberry leaf flavone and alkaloid composite |
| CN102204985A (en) * | 2011-06-10 | 2011-10-05 | 广东省农业科学院蚕业与农产品加工研究所 | Compound lychee seed hpyerglycemic particles and preparation method thereof |
| CN104435746B (en) * | 2014-11-05 | 2018-05-01 | 贵州赤水国礼金钗石斛发展有限公司 | Method for simultaneously extracting and preparing polysaccharide, flavone and alkaloid from dendrobium nobile lindl |
| CN104435746A (en) * | 2014-11-05 | 2015-03-25 | 贵州赤水国礼金钗石斛发展有限公司 | Method for simultaneously extracting polysaccharides, flavones and alkaloid from dendrobium stem |
| CN104940293A (en) * | 2015-06-15 | 2015-09-30 | 塔里木大学 | Method for extracting alkaloid, polysaccharide and flavone from mulberry leaves |
| CN104940293B (en) * | 2015-06-15 | 2018-01-09 | 塔里木大学 | A kind of method that alkaloid, polysaccharide and flavones are extracted from mulberry leaf |
| CN108785388A (en) * | 2017-04-26 | 2018-11-13 | 奥维嘉生物科技（北京）有限公司 | A kind of composite formula and preparation method treating and preventing diabetes and its complication |
| CN107721909A (en) * | 2017-11-02 | 2018-02-23 | 厦门福美科技有限公司 | DNJ, flavones, the method and system of polysaccharide are continuously extracted from moraceae plants |
| CN107721909B (en) * | 2017-11-02 | 2023-12-29 | 广西五和博澳药业有限公司 | Method and system for continuously extracting DNJ, flavone and polysaccharide from Moraceae plant |
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
| CN107951927A (en) * | 2017-12-15 | 2018-04-24 | 佛山市米良仓科技有限公司 | The extracting method of active material in medicine mulberry leaf |
| CN109463399A (en) * | 2018-11-20 | 2019-03-15 | 安徽省应用技术研究院 | A kind of anti-mulberry-leaf extract and preparation method thereof to eliminate aphis |
| CN113509484A (en) * | 2021-08-12 | 2021-10-19 | 天津大学 | Application of mulberry leaf polysaccharide in preparation of weight-losing medicine or weight-losing health-care product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101278977B (en) | 2012-05-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101278977B (en) | Method for extracting main active components of mulberry leaf and application of its extract | |
| EP2520305B1 (en) | Pharmaceutical composition including sunflower extract, preparative method and use thereof | |
| CN101623329B (en) | Method for extracting paper mulberry alkaloid and application of paper mulberry alkaloid | |
| CN101450166A (en) | Traditional Chinese medicine composition with blood-sugar function and preparation method thereof | |
| CN101744869B (en) | Compound traditional Chinese medicine extract composition for preventing and treating diabetes | |
| CN104173451B (en) | The application in blood sugar lowering medicine and health food of a kind of natural drug composition | |
| CN101757073B (en) | Medicinal composition containing atractylodes rhizome extractive | |
| CN104982597B (en) | A kind of multi-functional composite instant tea and its preparation method and application | |
| CN101991567A (en) | Application of three biflavone monomer components extracted from ginkgo leaves in preparing medicament of alpha-glucosidase inhibitor | |
| CN101966228B (en) | Application of mangnolia officinalis alkaloid | |
| CN101919901B (en) | Application of total aglycone of Gleditsia sinensis Lam in preparation of alpha-glucosidase inhibitor drugs | |
| CN102920785A (en) | Method for extracting total flavone from guava leaves | |
| CN105640971B (en) | Application of the total saposins in terms of preparing auxiliary hyperglycemic drug in prematurity Fructus Monordicae extract | |
| CN102526138B (en) | Composition of active components from fresh purslane for decreasing blood sugar and preparation method | |
| CN112159451A (en) | Gynostemma pentaphylla saponin extract and preparation method thereof | |
| CN105030914B (en) | Application of the Ligustrum robust Folum Ilicis extract in alpha-glucosidase restrainer | |
| CN1565467B (en) | Use of cornel and its extract in preparation alpha-glucosidase inhibitor medicine | |
| CN101890063B (en) | Chinese medicament for reducing blood sugar and preparation method thereof | |
| CN102579530A (en) | Preparation method of aralia taibaiensis total saponin having diabetes mellitus resisting effect and medicament | |
| CN110144015B (en) | Method for synchronously extracting and purifying ganoderan, ganoderma triterpenic acid and amino acid | |
| CN104547026B (en) | Preparation method and application of salvia miltiorrhiza leave pseudo-ginseng extract | |
| CN101450162A (en) | Traditional Chinese medicine composition with blood-sugar reduction function and preparation method thereof | |
| CN112656828A (en) | Pseudo-ginseng leaf product | |
| CN101732370B (en) | Method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze | |
| CN1887309B (en) | Extraction of effective red sage components for preventing and treating osteoporosis and depression |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: GUANGZHOU TANGBAIFU BIOLOGY SCIENCE AND TECHNOLOGY Free format text: FORMER OWNER: CHENGDU INST. OF BIOLOGY, CHINESE ACADEMY OF SCIENCES Effective date: 20091204 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20091204 Address after: 1001, unit 268, Dongfeng Middle Road, Guangzhou, Guangdong (Guangzhou Trading Plaza), zip code: 510030 Applicant after: Guangzhou Tang Bai Biologic Science and Technology Co., Ltd. Co-applicant after: Chendu Biology Inst., Chinese Academy of Sciences Address before: Four, 9 South Renmin Road, Sichuan, Chengdu Province, China: 610041 Applicant before: Chengdu Institute of biology, Chinese Academy of Sciences Co-applicant before: Guangzhou Runxing Biotech Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20170331 |