CN107951927A - The extracting method of active material in medicine mulberry leaf - Google Patents
The extracting method of active material in medicine mulberry leaf Download PDFInfo
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- CN107951927A CN107951927A CN201711354749.6A CN201711354749A CN107951927A CN 107951927 A CN107951927 A CN 107951927A CN 201711354749 A CN201711354749 A CN 201711354749A CN 107951927 A CN107951927 A CN 107951927A
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- mulberry leaf
- active material
- extracting method
- mulberry
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The present invention proposes a kind of extracting method of active material in medicine mulberry leaf, includes the following steps：S1, accurately weigh dried drug Mulberry Leaf 300.0g, by 1：20 solid-liquid ratios add 6.0L petroleum ethers, 70 DEG C of reflux 4h, filtering；S2, filter residue press solid-liquid ratio 1:20 add 6.0L65% ethanol, soak one day night, and filtering, filtrate decompression is concentrated to give mulberry extract；S3, mulberry extract pass through resin separation purification, respectively obtain three kinds of crude extracts of alkaloid, flavones and polysaccharide.The chemical composition and stable content for the active material that extracting method is extracted in the present invention, have repeatability, ensure that the reliability of middle medicine material.
Description
Technical field
The present invention relates to belonging to field of traditional Chinese medicine extraction, the extracting method of active material in more particularly to a kind of medicine mulberry leaf.
Background technology
Medicine mulberry belongs to Moraceae, Morus, black mulberry kind (Morus Nigra L.), due to southern Xinjiang, life in Plant Taxonomy
State environment is unique, and the sunshine-duration is long, day and night temperature is big, arid, and it is nature the formation of medicine mulberry is had somatic chromosome ploidy
22 times of your dilute Morus alba Resource Developments of body (2n=22x=308), it is regional to be distributed mainly on Aksu of Xinjiang and field and Keshen etc. at present, is
Xinjiang region is in the unique mulberry germ plasm resource in the whole nation.Modern pharmacology and chemical constitution study show that mulberry leaf have drop blood
A variety of pharmacological activity such as sugared, anti-oxidant, antiviral, wherein alkaloid and Flavonoid substances are hypoglycemic work main in mulberry leaf
Property component.Yu Ling warblers etc. report that blood glucose is down to 10.2mmol/ by 18.4mmol/L to after diabetes rat gavage total flavonoids from mulberry tree leaf
L (P ＜ 0.001), blood sugar reducing function is notable.Chen Jianguo etc. is pointed out：Mulberry leaf polysaccharide can adjust glycometabolism, reduce blood glucose, improve sugar
Urinate the effect of disease symptoms.The isolated alkaloid component from mulberry tree for the first time such as Yagi in 1976, buys and carries according to bright grade once to medicine
Alkaloid component in mulberry leaf piece is detected, and it is 1-DNJ (1- to find its main component
Deoxynojirimycin, DNJ), content 1.8933mg/g.In addition, some researches show that total flavonoids from mulberry tree leaf can also suppress double
Anase activity, so as to have significant hypoglycemic effect.And flavonoids is combined with DNJ and can more effectively be suppressed on blood glucose
Rise, show that flavonoids and mulberry leaf DNJ have certain synergistic effect.And Xiangya Medical College, Zhongnan Univ department of physiology to one kind with mulberry
Leaf is studied for the health-caring product capable of reducing blood sugar of primary raw material, it has been found that other compositions have one in mulberry leaf polysaccharide, alkaloid and product
Fixed collaboration compatibility effect, above research are that the random concentration combination method used is made to study the collaboration between different material
With, but how collaboration, addition or the antagonism between each component of quantitative analysis do not use statistical method.Generally use at present
Chou-Talalay (also known as middle position drug effect method) formula evaluates drug synergism effect, Chou-Talalay be by Chou and
" Compu Syn ", are the medicines being widely recognized as at present to the 3rd generation Combined effects amount-effect analysis software that Talalay is developed
Thing synergistic effect quantitative analysis method, the formula mainly carry out quantitative description medicine with association index (combination index, CI)
Correlation between thing.
The content of the invention
In view of this, in a kind of medicine mulberry leaf proposed by the present invention active material extracting method, it is with Xinjiang characteristic medicine mulberry
Mulberry leaf are material, on the basis of the established integrated extraction process in this laboratory, by suitably optimizing, obtain chemical composition and contain
Measure the crude extract of alkaloid basicly stable, that there is hypoglycemic activity, flavones and polysaccharide.
The extracting method of active material, includes the following steps in a kind of medicine mulberry leaf：
S1, accurately weigh dried drug Mulberry Leaf 300.0g, by 1：20 solid-liquid ratios add 6.0L petroleum ethers, 70 DEG C of reflux
4h, filtering；
S2, filter residue press solid-liquid ratio 1:20 add 6.0L65% ethanol, soak one day night, and filtering, filtrate decompression is concentrated to give
Mulberry extract；
S3, mulberry extract pass through resin separation purification, respectively obtain three kinds of crude extracts of alkaloid, flavones and polysaccharide.
In the step S3, mulberry extract is first eluted with water after macroporous resin treatment, obtains coarse biometric alkali and thick more
Sugar, then obtain crude flavonoid powder with ethanol elution；Coarse biometric alkali and Thick many candies carry out separating treatment again.
Coarse biometric alkali and Thick many candies are handled by ion exchange resin, are successively eluted with water and ammonium hydroxide, obtain Thick many candies and thick life
Alkaloids.
The chemical composition and stable content for the active material that extracting method is extracted in the present invention, have repeatability, ensure that
The reliability of middle medicine material.
Embodiment
The present invention is got information about to allow those skilled in the art to become apparent from, the present invention will be made below further
Explanation.
The extracting method of active material, includes the following steps in a kind of medicine mulberry leaf：
S1, accurately weigh dried drug Mulberry Leaf 300.0g, by 1：20 solid-liquid ratios add 6.0L petroleum ethers, 70 DEG C of reflux
4h, filtering；
S2, filter residue press solid-liquid ratio 1:20 add 6.0L65% ethanol, soak one day night, and filtering, filtrate decompression is concentrated to give
Mulberry extract；
S3, mulberry extract pass through resin separation purification, respectively obtain three kinds of crude extracts of alkaloid, flavones and polysaccharide.
In the step S3, mulberry extract is first eluted with water after macroporous resin treatment, obtains coarse biometric alkali and thick more
Sugar, then obtain crude flavonoid powder with ethanol elution；Coarse biometric alkali and Thick many candies carry out separating treatment again.
Coarse biometric alkali and Thick many candies are handled by ion exchange resin, are successively eluted with water and ammonium hydroxide, obtain Thick many candies and thick life
Alkaloids.
The chemical composition and stable content for the active material that extracting method is extracted in the present invention, have repeatability, ensure that
The reliability of middle medicine material.
The present invention using medicine mulberry leaf as experimental raw, by integrate extraction process, obtain three kinds of stable contents crude extract into
Point, its inhibitory action to ɑ-glucuroide of follow-up study, and between carrying out different crude extracts composition to ɑ-glucuroide
The research of inhibitory activity.Extracted using ethanol, column chromatography separating purification the methods of, obtain mulberry leaf in there is potential hypoglycemic activity
Material flavones, polysaccharide and alkaloid crude extract；Suppress three kinds of crude extracts of model evaluation to ɑ-grape using alpha-glucosidase
The inhibitory action of glycosidase, and statistics is further carried out to the interaction different crude extracts using Compu Syn softwares
Analysis.Test result indicates that：Flavones, alkaloid, polysaccharide crude extract are respectively provided with alpha-glucosidase inhibitory activity in mulberry leaf, and
The inhibitory activity of alkaloid crude extract is significantly higher than positive control (acarbose), and polysaccharide crude extract is under high concentration effect
With alpha-glucosaccharase enzyme inhibition, it is directly proportional to more Sugar concentrations to show as inhibitory action；Used in this experiment
When mass concentration is mutually combined with three kinds of crude extracts in dosage ratio, flavones+alkaloid combination, polysaccharide+alkaloid combination, performance
For antagonism；Flavones+polysaccharide combination, flavones+polysaccharide+alkaloid combination, to ɑ-glucuroide under high mass concentration
Show as acting synergistically.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention god.
Claims (3)
1. the extracting method of active material in a kind of medicine mulberry leaf, it is characterised in that include the following steps：
S1, accurately weigh dried drug Mulberry Leaf 300.0g, by 1：20 solid-liquid ratios add 6.0L petroleum ethers, 70 DEG C of reflux 4h, mistake
Filter；
S2, filter residue press solid-liquid ratio 1:20 add 6.0L65% ethanol, soak one day night, and filtering, filtrate decompression is concentrated to give mulberry leaf
Medicinal extract；
S3, mulberry extract pass through resin separation purification, respectively obtain three kinds of crude extracts of alkaloid, flavones and polysaccharide.
2. the extracting method of active material in a kind of medicine mulberry leaf according to claim 1, it is characterised in that the step S3
In, mulberry extract is first eluted with water after macroporous resin treatment, obtains coarse biometric alkali and Thick many candies, then obtained with ethanol elution
Crude flavonoid powder；Coarse biometric alkali and Thick many candies carry out separating treatment again.
3. the extracting method of active material in a kind of medicine mulberry leaf according to claim 2, it is characterised in that coarse biometric alkali and
Thick many candies are handled by ion exchange resin, are successively eluted with water and ammonium hydroxide, are obtained Thick many candies and coarse biometric alkali.
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| CN201711354749.6A CN107951927A (en) | 2017-12-15 | 2017-12-15 | The extracting method of active material in medicine mulberry leaf |
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| CN201711354749.6A CN107951927A (en) | 2017-12-15 | 2017-12-15 | The extracting method of active material in medicine mulberry leaf |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114621129A (en) * | 2020-12-11 | 2022-06-14 | 沈阳药科大学 | Antitumor compound and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278977A (en) * | 2008-03-31 | 2008-10-08 | 中国科学院成都生物研究所 | Method for extracting main active components of mulberry leaf and application of its extract |
-
2017
- 2017-12-15 CN CN201711354749.6A patent/CN107951927A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278977A (en) * | 2008-03-31 | 2008-10-08 | 中国科学院成都生物研究所 | Method for extracting main active components of mulberry leaf and application of its extract |
Non-Patent Citations (1)
| Title |
|---|
| 郝蒙蒙等: "药桑叶中活性物质的提取及对α-葡萄糖苷酶的抑制作用", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114621129A (en) * | 2020-12-11 | 2022-06-14 | 沈阳药科大学 | Antitumor compound and preparation method and application thereof |
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Application publication date: 20180424 |