CN101244095A - New purpose of durio zibethinus L. shell extract - Google Patents
New purpose of durio zibethinus L. shell extract Download PDFInfo
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Abstract
The invention provides a medicine, a healthy food and a food additive with high safety and no side effect which are used to resist oxidation and stress, and eliminate a plurality of clinical symptoms due to stress load or body and mind fatigue, and are made from Durio zibethinus Murr shell or extract of Durio zibethinus Murr shell. Particularly, the invention provides the medicine, the healthy food, the food additive and a composition for improving body state, which is available for long-term use with high safety and no side effect; the medicine, the healthy food and the food additive are made from Durio zibethinus Murr shell or extract of Durio zibethinus Murr shell, and aim to improve levels of plasma Alanine amino ALT and MDA under stress load. Additionally, the invention proves that the medicine, the healthy food, the food additives and the composition made by extractions from Durio zibethinus Murr shell or extract of Durio zibethinus Murr shell can improve and relieve a plurality of clinical symptoms caused by oxidation resisting and anti-stress load.
Description
Technical field:
The present invention relates to extracted form natural plant and application.
Background technology:
Durio Zibethinus murr (Durio zibethinus Murr) claim Fructus nephelii topengii again, is the fruit of the long green arbor Durio Zibethinus murr of Bombacaceae durian genus, the tropical large-scale fresh fruit that local flavor is unusual.Originate in India, the Malay Peninsula one band, at first pass to the tropic countries in Philippine, India, Sri Lanka and the Indonesia of closing on or the like Southeast Asia and South Asia from the original producton location, in recent years, also there is introducing and planting in a small amount in China Guangxi, south of Yunnan area.Be ovoid shape, generally heavily about two kilograms, the outside is lignitoid's duricrust, interior mark room, every room have three, four as the egg yolk seeds in size, have ten to 15 pieces, it is exactly sarcocarp that one deck ointment is wrapped up in the seed outside, be milk yellow, its fruit shape is huge, and sarcocarp has special strong fragrant and sweet local flavor, after eating, taste is sweet and spray, and between the lasting lips and teeth, is described as " king in the fruit ".
The Chemical Constituents method research to Durio Zibethinus murr also is a main aspect of Durio Zibethinus murr research, people have adopted HSSPME, SDE, SD, methods such as GC-MS have been carried out comparatively comprehensively and the analysis of system the different parts such as seed, sarcocarp, endothelium, crust and trunk of different cultivars Durio Zibethinus murr, find that its nutritive value is abundant, its different composition also has a lot of potential pharmacological activities.Nutrition shows with the chemical composition analysis result: the protein content in the durian reaches 2.7%, and (different amino acid contents are respectively: aspartic acid Asp2.83mg/g, isoleucine Ile0.87mg/g, threonine Thr0.87mg/g, leucine Leu1.78mg/g, serine Ser1.00mg/g, tyrosine Tyr0.49mg/g, glutamic acid Glu6.76mg/g, phenylalanine Phe 1.02mg/g, alanine Ala 0.82mg/g, histidine Hi s0.45mg/g, cystine Cys0.75mg/g, arginine Arg1.38mg/g, valine Val1.19mg/g, proline Pro1.68mg/g, glycine Gly1.38mg/g, lysine Lys1.46mg/g, methionine Met0.2mg/g), fat is that 4.1% (in the kind skin of Durio Zibethinus murr, unsaturated fatty acid contains 48.98% approximately, wherein 18 carbon diluted acids contain 36%, ten eight carbon, two diluted acids approximately and contain 10% approximately.The content of unsaturated fatty acid is 51% in endocarp, and wherein to contain 37.07%, ten eight carbon, two diluted acids be 8.03% to 18 carbon diluted acids.In outer peel, unsaturated fatty acid content is 56.37%, and wherein 18 carbon diluted acids contain 37.09%, ten eight carbon, two diluted acids and contain 16.28%.Therefore, Durio Zibethinus murr is except seed has high edibility and medical value, common kind skin, endocarp and outer peel as refuse, also contain multiple composition to the human body beneficial, in addition, in these unsaturated fatty acids, as: oleic acid, linoleic acid etc., all can be used for making emulsifying agent, polishing agent, soap etc., can further develop.), dietary fiber 0.8%, ash 0.2%, carbohydrate 9.7%.The content of Durio Zibethinus murr mineral element is abundant, be 4.53mg/g wherein with the extra high K of having content, Ca content is 2.17mg/g, other is 0.256mg/g for Zn content, Fe content is 0.041mg/g, and Mg content is 0.214mg/g, and Na content is 0.136mg/g, Mn content is 0.011mg/g, and Cu content is 0.011mg/g.The vitamin content retinol of Durio Zibethinus murr (Vitamin A) is 3.86ug/g, and ascorbic acid (Vitamin C) is 890ug/g, and thiamine (Vitamin B1) is 0.10ug/g, and riboflavin (Vitamin B2) is 0.90ug/g, and beta-carotene is 0.18ug/g.Composition in the Durio Zibethinus murr shell, mainly contain and contain multiple esters, ketone, hydro carbons and sulfur-containing compound, its main fragrance component is the esters of 3-hydroxyl-butanone-2 and number of C 4-C8, also contain some higher fatty acid ethyl esterses, as: ethyl palmitate (1.51%) and 18 carbon, olefin(e) acid ethyl ester (3.03%) is not though these esters have outstanding fragrance, but to the fragrance that keeps Durio Zibethinus murr itself with form its aftertaste certain effect is arranged; Volatile ingredient nonadecane (5.40%), octadecane (5.09%), heptadecane (4.22%), eicosane (3.95%), 11-butyl-docosane (3.78%), nonacosane (3.68%), heptacosane (3.63%), docosane (2.84%), 3,5-dihydroxy-benzoic acid (2.65%), the rate of reflecting out account for (95.97%) of full oil.
Aspect agronomy, the pollination of Durio Zibethinus murr has no mode, and the different cultivars Durio Zibethinus murr is for artificial self-pollination, and cross pollination etc. have different effects; Aspect breeding, Thailand at first cultivated stingless Durio Zibethinus murr in 1984; Malaysian research and development academy cultivated the Durio Zibethinus murr Hybrid for the first time by the method for system's selection in 1991, was respectively MDUR78, MDUR79 and MDUR88, and the common feature of these kinds is fruit quality height, all anti-anthrax; Also have the peasant to cultivate purple, redness and orange Durio Zibethinus murr in addition, some kind has the special fragrance of approximate jackfruit, and its fruit is littler than general kind, and does not have special abnormal smells from the patient.Aspect the results storage, except low temperature may cause the influence such as injures and deaths of Durio Zibethinus murr, 75% humidity was the most obvious and it is deposited the ripe influence of not having for Durio Zibethinus murr weight saving; The plantation aspect comprises soil environment, management (control of bloom pollination, disease and pest) before the results, the management of results back and transportation, storage, sale etc.; Also have from the influence of ecological angle research Durio Zibethinus murr and surrounding; The plant classification scholar adopts RFLP to determine the kind problem of Durio Zibethinus murr now.
Aspect pomegranate food, eat except traditional aquatic foods, make Durio Zibethinus murr sugar, Durio Zibethinus murr cake, coconut palm slurry Durio Zibethinus murr etc., some researcher incorporates the peat-reek of Durio Zibethinus murr Durio Zibethinus murr pineapple wine and the Durio Zibethinus murr Fructus Mangifera Indicae wine of making unique flavor in pineapple wine and the Fructus Mangifera Indicae wine; Because the special taste of Durio Zibethinus murr is extensive use of it as flavoring agent gradually Malaysian people; Also the handlebar durian peel is that raw material is cultivated edible fungi as the culture medium of edible fungi, obtains high-quality mushrooms.Both helped environmental protection, and reduced and pollute; Can turn waste into wealth, produce considerable economic again.It utilizes the durian peel benign cycle, realizes comprehensive economic results in society most possibly.Can be widely used in the cultivation production of various edible fungi.
Aspect the applied research of Durio Zibethinus murr, because the Durio Zibethinus murr shell has low cost, excellent mechanical intensity, performances such as lower heat transfer property and conduction so be applied to more and more widely in the construction material, are especially used with Exocarpium cocois (Cocos nucifera L); In addition, the someone begins the Durio Zibethinus murr shell is used as making a kind of good raw material of furniture now.Aspect chemical purposes research, handlebar Durio Zibethinus murr shell is made the research of active carbon one class adsorbents adsorb waste water dyestuff; The somebody replaces the Durio Zibethinus murr shell silica gel also to obtain some effects as desiccant.
The medical value of Durio Zibethinus murr also can not be despised, ancient books record it can " quenching the thirst, it be tired to separate, and the QI invigorating of calming the nerves is made us Yue Ze ", " invigorating the spleen and replenishing QI makes us not hungry, spry and light ".Juice and the leaf of Durio Zibethinus murr tree also are used as medicine, the energy subduing swelling and detoxicating; Leaf and bark dry to be worn into powder and can apply wound, is a kind of effective first aid hemorrhage; Modern medicine study, the proteolytic enzyme that from Durio Zibethinus murr juice and peel, extracts, clinical edema and the antibiotic medicine done.The hydrolysis of energy intensive aspect inner fibrin can be dissolved blood clotting after oral, improves the local circulation of body fluid, thereby inflammation and edema are eliminated.Durio Zibethinus murr protease and antibiotics, chemotherapeutics and usefulness can promote the infiltration of medicine to focus, can be used for the disease such as inflammation, edema, thrombosis due to a variety of causes, as bronchitis, acute pneumonia, mastitis, retinitis etc.; The crust extracting solution of Durio Zibethinus murr also has curative effect preferably for senile Pruritus; The calendar year 2001 research of Japan reports that also durian has the certain protection effect to hepatic injury; Thailand in 2004 discovers that polyphagia has tangible help with Durio Zibethinus murr to reducing post-prandial glycemia; Up-to-date research finds that also the water soluble polysaccharide extract of Durio Zibethinus murr has good inhibitory effect for haemolysis.But so far the pharmacology activity research of the anti-stress aspect of Durio Zibethinus murr shell is not seen any report as yet.
Durio Zibethinus murr has also been ignored on stream to its pharmacologically active protection of Intellectual Property Rights.Requirement on any intellectual property is done in domestic and Europe, the United States, Japan, the Southeast Asia still biological activity of foot couple Durio Zibethinus murr shell.The existing patent of Durio Zibethinus murr shell also only is the protection to edible aspect at present, and just few in application facet researchs such as prevent disease, healthy function, medicine, health food and cosmetics for the Durio Zibethinus murr shell, intellectual property more is not effectively protected.
Along with the progress of science and technology and the quickening of rhythm of life, people need bear all pressure from different aspect in order to adapt to the intensive day by day competition of society, make people usually be in stress state, by stress and the problem that causes is also following.Stress be the stimulation that body is subjected to any factor, identical whole body defense reaction and the adaptation irrelevant all occur with stimulating kind.That propose this notion at first is Canadian Hans Selye (1935).The performance of the stress of its description is that gastrointestinal mucosal wastes mashed, atrophy of thymus gland, adrenal gland's hypertrophy.Nearest studies show that by stress, the bundle load mode of bringing out the mice oxidative stress especially, the mental pressure that can cause the hepatic injury of animal and cause animal; And theory of Chinese medical science thinks that the caused mechanism of qi of various stimulus changes, and is mainly adjusted by liver.At psychological aspects for emotion changes, especially when regulating the various variation that feelings will factor causes, liver decisive role [2].The organismic internal environment steady statue that the liver function of this prompting Chinese medicine and the nerve-endocrine-immune system of western medicine are represented is closely related.
In addition, we can say to stress load body occurred is fatigue reaction, also can belong to the traditional Chinese medical science " overstrain " category.Secular fatigue reaction is a kind of sub-health state.A global investigation of The World Health Organization (WHO) shows that the people of true health only accounts for 5%, and the people who suffers from disease accounts for 20%, 75% people and then is in sub-health state.Subhealth state (sub-health) claim the third state again, also claim gray states, sick before state, subclinical phase, preclinical phase, incubation period etc., be a kind of state between health and disease, the slight but existing potential pathological information of clinical symptoms.Its topmost form of expression is chronic fatigue syndrome (chronic fatigue syndrome CFS).Chronic fatigue syndrome be a kind of be the systemic disease of main performance with the chronic fatigue, be to show as main syndrome so that fatigue, low grade fever, difficult concentrating, hypomnesis, sleep disorder and depression etc. are non-specific.
For the foregoing reasons, it is raw material with Durio Zibethinus murr shell and Durio Zibethinus murr shell extract that the present invention provides a kind of, is anti-stress, resisting fatigue, the anti-infection activity material of the scope of application with pharmaceuticals, health food and food additive.Definitely say, the present invention is by the indexs such as liver function under stress loading with raising, providing a kind of is raw material with Durio Zibethinus murr shell and Durio Zibethinus murr shell extract, improves fuselage state and can take for a long time, safely, the pharmaceuticals, health food, food additive and the constituent thereof that have no side effect.In addition, the present invention demonstrate,proves Durio Zibethinus murr shell and the suggested effect alive of Durio Zibethinus murr shell extract and can be used to alleviate and treat various clinical symptoms because of the low cause of anti-stress, also can be used as the medicine, health food, food additive and the constituent thereof that strengthen the body endophylaxination because of antioxidation.
Summary of the invention:
The object of the present invention is to provide with Durio Zibethinus murr shell and Durio Zibethinus murr shell extract is raw material, prepare a kind of safe, being used to of having no side effect prevent because of variously stress load, health food, therapeutic agent, chemotherapy adjuvant or its constituent of various diseases that factor such as oxidation is brought out.Described above-mentioned therapeutic agent or its constituent should comprise the Durio Zibethinus murr shell and the Durio Zibethinus murr shell extract of dose therapeutically effective, and pharmaceutically acceptable pharmaceutical carrier.This therapeutic agent or its constituent particularly suppress loading or the prevention of the tired clinical symptoms that causes, improve effectively, having or alleviate effect.
It is raw material that another goal of the invention of the present invention is to provide with Durio Zibethinus murr shell and Durio Zibethinus murr shell extract, and preparation can take for a long time, the purposes of safe, the health food, food and the food additive that have no side effect.The daily food that contains careless Durio Zibethinus murr shell and Durio Zibethinus murr shell extract of taking can alleviate and prevent the flu of the various causes of stress loading, various acute or clinical symptoms such as chronic inflammatory disease, various complication.
Durio Zibethinus murr shell of the present invention and Durio Zibethinus murr shell extract are to adopt one or two or more kinds any mixed solvent of lower alcohols such as direct pulverization or hot water and water and methanol, ethanol, acetone isopolarity solvent to extract.Again because commercially available Durio Zibethinus murr shell and Durio Zibethinus murr shell many be raw material with the water extract, so general Mining get final product with water extraction, also the lower alcohols of available variable concentrations, acetone isopolarity solvent or any mixed solvent extract.When extracting, along with the interpolation of alcohol, the extraction of active ingredients rate can increase to some extent in Durio Zibethinus murr shell and the Durio Zibethinus murr shell.Although the water extraction effect, also can reach with alcohol extraction and follow the example of suitable result along with water temperature increase and the prolongation of extraction time not as with the solvent extraction of having added certain alcohol.Therefore, for extraction of active ingredients in Durio Zibethinus murr shell and the Durio Zibethinus murr shell, said extracted method and combination in any thereof all can obtain extract of the present invention.
In view of Durio Zibethinus murr shell and Durio Zibethinus murr shell extract are to develop as final products with medicament, health food or food additive, select water or aquiferous ethanol to be advisable so consider to extract solvent from safety perspective as far as possible.The ratio of Durio Zibethinus murr shell and Durio Zibethinus murr shell and solvent does not have specific limited during extraction, but the ratio of general Herba Pileae Scriptae and solvent is advisable with 1: 1～50 scopes.The present invention does not have specific limited to extracting temperature, and is all applicable to the solvent boiling point scope from room temperature.Certainly room temperature, normal pressure and in the solvent boiling point scope, extract ideal.Extraction time, scope could from 10 seconds to 24 hours.When extracting, also can in solvent, add sodium bicarbonate, SODIUM ASCORBATE materials such as (sodium ascorbate) as required.These extracting method can not influence the biological activity that Durio Zibethinus murr shell and Durio Zibethinus murr shell extract had originally.
The use amount of Durio Zibethinus murr shell provided by the invention and Durio Zibethinus murr shell extract is generally in 0.001% to 100% weight ratio, but beverage interpolation concentration is generally comparatively suitable in 0.01% to 50% (W/W) scope usually.In this concentration range, Durio Zibethinus murr shell and Durio Zibethinus murr shell extract are suitable for directly taking, and are accepted by consumer easily.Certainly, along with the increase of addition, pained or special stink sensation may appear as the careless Durio Zibethinus murr shell of additive and Durio Zibethinus murr shell extract.Its solution can require according to the psychology of market demand and consumer to change dosage form, for example uses dosage forms such as tablet, granule, capsule.Low concentration Durio Zibethinus murr shell and Durio Zibethinus murr shell extract solution can be used as daily beverage and offer consumer.
Durio Zibethinus murr shell provided by the invention and Durio Zibethinus murr shell extract can be put on market by multiple peroral dosage form, are used to the various clinical symptoms of preventing, improving and treating stress and stress load and cause.Certainly, Durio Zibethinus murr shell and Durio Zibethinus murr shell extract also can combine preparationization then with pharmaceutical carriers such as excipient commonly used.Also can add preparation additives such as some antiseptic, antioxidant (antioxidant), coloring agent, sweeting agent in case of necessity.Relatively Shi Yi Durio Zibethinus murr shell and Durio Zibethinus murr shell extract excipient generally consider to have lactose, white sugar, D-mannitol, starch, crystalline cellulose etc.Lubricant generally should adopt magnesium stearate, calcium stearate, Pulvis Talci etc.Solvent uses Purified Water, ethanol, propylene glycol etc. usually.Antiseptic generally adopts methaform, benzylalcohol, dihydroxy acetic acid etc.Antioxidant is more suitable with sulphite, ascorbic acid etc. usually.
Durio Zibethinus murr shell provided by the invention and Durio Zibethinus murr shell extract, can its prototype, extract or powder add to be used in the goods oral.Durio Zibethinus murr shell and Durio Zibethinus murr shell extract also can combine with other beverage raw material and make certain beverage product, as Durio Zibethinus murr shell and Durio Zibethinus murr shell extract beverage, soda pop, fruit drink, lactobacillus beverage, sports drink, soyabean milk etc.Grass Durio Zibethinus murr shell and Durio Zibethinus murr shell extract product it is also conceivable that daily breads such as making cookies, chocolate-like, confection class, chewing gum class, snack class, fruit jelly snack categories, bread, bean curd, yoghourt.Durio Zibethinus murr shell that provides among the present invention and Durio Zibethinus murr shell extract also can prototypes, aqueous solution dilution back uses as skin preparations for extenal use.Can certainly make certain preparation by combining, the consumption in cosmetics, pharmaceuticals market is provided as dosage forms such as aerosol, liquid preparation, extract, suspensoid, Emulsion, ointment, semifluid preparation, liniment, lotions with pharmaceutical carrier.
The invention provides Durio Zibethinus murr shell and Durio Zibethinus murr shell extract as anti-stress, resisting fatigue, alleviate and prevent flu that a variety of causes causes, various acute or clinical symptoms medicine such as chronic inflammatory disease, various complication and beverage and proved by following experiment.In the pharmacological evaluation of use to improve restraining the load animal model, Durio Zibethinus murr shell and Durio Zibethinus murr shell extract reach 100mg/kg when above at dispensing dosage, promptly demonstrate effective improvement in response to swashing the various symptoms down that load causes.The dosage of Herba Pileae Scriptae and Herba Pileae Scriptae extract among the present invention can be regulated according to application person's conditions such as health status, age, sex and body weight.Adult's average weight is as calculating with 60Kg, and Durio Zibethinus murr shell and Durio Zibethinus murr shell extract day oral dose generally can be considered in 0.5g to 100g scope, part vic.Certainly there are not problems such as side effect more than the 100g yet.
The present invention proves that independent oral Durio Zibethinus murr shell and Durio Zibethinus murr shell extract can effectively be regulated in response to swashing the various livers that cause, the variation of blood parameters.In addition, the present invention estimates Durio Zibethinus murr shell anti-stress effect and liver protection function effect to restrain stress induction Mouse Liver hypofunction as animal test model.The result shows that Durio Zibethinus murr shell and Durio Zibethinus murr shell extract show significant improvement effect on the statistics to restraining stress induction Mouse Liver functional activity, confirms that based on The above results the present invention sets up.
The specific embodiment:
The present invention can be elaborated by following examples, but is not limited only to following examples.
The preparation of embodiment one, Durio Zibethinus murr shell extract: dry Durio Zibethinus murr shell 100 grams, use the pulverizer micropowder broken, reclaim the Durio Zibethinus murr shell of fine-powdered and add 4000ml water 80 ℃ of heat under 30 minutes pressurized conditions and extract three times.Then with extracting solution after gauze filters, after 10000rpm, centrifugalize in 15 minutes, reclaim the supernatant lyophilization.The above-mentioned lyophilization extract of the about 28-32% Durio Zibethinus murr of response rate shell is used for the following example of the present invention.
Embodiment two, Durio Zibethinus murr shell extract improve to restrain and stress cause the disorderly effect of alanine aminotransferase (ALT): experiment use that Guangdong Medical Lab Animal Center buys 6 age in week male mouse of kunming, 23 ± 2 ℃ of raising temperatures, lighting hours 12h hour/day (7:00-19:00) raises and is used for experiment after a week.Mice is divided into the blank group at random, restrains the Stress model group, positive drug control group (vitamin C 250mgkg
-1), low dosage Durio Zibethinus murr shell extract group (100mgkg
-1), middle dosage Durio Zibethinus murr shell extract group (300mgkg
-1), high dose Durio Zibethinus murr shell extract group (900mgkg
-1) wait the administration group, every group of 7 mices are divided into 6 groups.Matched group and contained load model group are all irritated stomach with the volume distilled water, every day 1 time, 30 minutes laggard administrative detention bundles of the 4th day model group and Durio Zibethinus murr shell extract and vitamin C administration group mouse stomach stress 18 hours, matched group is prohibited water fasting 18 hours, all are with the mice etherization, extract blood, centrifugal treating is measured plasma A LT level with reitman-frankel method.The result show proof Durio Zibethinus murr shell behind the oral administration extract can improve restrain stress cause ALT raise, make the ALT value drop to normal level (Fig. 1 for the Durio Zibethinus murr shell extract to restrain stress mice plasma ALT influence, " * " expression and blank group be p＜0.05 relatively).
Embodiment three, Durio Zibethinus murr shell extract promote to restrain stress cause the effect of mice plasma malonaldehyde (MDA) abnormal restoring: experiment use that Guangdong Medical Lab Animal Center buys 6 age in week male mouse of kunming; 23 ± 2 ℃ of raising temperatures; lighting hours 12h hour/day (7:00-19:00) raises and is used for experiment after a week.Mice is divided into the blank group at random, restrains the Stress model group, positive drug control group (vitamin C 250mgkg
-1), low dosage Durio Zibethinus murr shell extract group (100mgkg
-1), middle dosage Durio Zibethinus murr shell extract group (300mgkg
-1), administration group (900mgkg such as high dose Durio Zibethinus murr shell extract group
-1), every group of 7 mices are divided into 6 groups.Matched group and contained load model group are all irritated stomach with the volume distilled water, every day 1 time, 30 minutes laggard administrative detention bundles of the 4th day model group and Durio Zibethinus murr shell extract and vitamin C administration group mouse stomach stress 18 hours, matched group is prohibited water fasting 18 hours, all are with the mice etherization, extract blood, centrifugal treating is measured contents of mda with the TBARS method.The result represents, prove and with the Durio Zibethinus murr shell extract can improve restrain stress cause mice plasma MDA increase (Fig. 2 for the Durio Zibethinus murr shell extract to restrain stress mice plasma MDA influence, " * " expression and blank group be p＜0.05 relatively).
Embodiment four, Durio Zibethinus murr shell extract to restrain stress cause the disorderly improvement effect of Mouse Liver paddy light liver peptidase (GSH): experiment use that Guangdong Medical Lab Animal Center buys 6 age in week male mouse of kunming, 23 ± 2 ℃ of raising temperatures, lighting hours 12h hour/day (7:00-19:00) raises and is used for experiment after a week.Mice is divided into the blank group at random, restrains the Stress model group, positive drug control group (vitamin C 250mgkg
-1), low dosage Durio Zibethinus murr shell extract group (100mgkg
-1), middle dosage Durio Zibethinus murr shell extract group (300mgkg
-1), high dose Durio Zibethinus murr shell extract group (900mgkg
-1) wait the administration group, every group of 7 mices are divided into 6 groups.Matched group and contained load model group are all irritated stomach with the volume distilled water, every day 1 time, 30 minutes laggard administrative detention bundles of the 4th day model group and Durio Zibethinus murr shell extract and vitamin C administration group mouse stomach stress 18 hours, matched group is prohibited water fasting 18 hours, all are with the mice etherization, take out GSH content results proof Durio Zibethinus murr shell extract that hepatic tissue measures hepatic tissue with the HPLC method can improve preferably restrain the liver GSH disorder that stress cause (Fig. 3 for the Durio Zibethinus murr shell extract to restrain stress mice Mouse Liver paddy light liver peptidase system influence, " * " expression is compared p＜0.05 with the blank group).
Embodiment five, Durio Zibethinus murr shell extract to restrain stress cause Mouse Liver nitric oxide (NO) improvement of increasing: experiment use that Guangdong Medical Lab Animal Center buys 6 age in week male mouse of kunming, 23 ± 2 ℃ of raising temperatures, lighting hours 12h hour/day (7:00-19:00) raises and is used for experiment after a week.Mice is divided into the blank group at random, restrains the Stress model group, positive drug control group (vitamin C 250mgkg
-1), low dosage Durio Zibethinus murr shell extract group (100mgkg
-1), middle dosage Durio Zibethinus murr shell extract group (300mgkg
-1), high dose Durio Zibethinus murr shell extract group (900mgkg
-1) wait the administration group, every group of 7 mices are divided into 6 groups.Matched group and contained load model group are all irritated stomach with the volume distilled water, every day 1 time, 30 minutes laggard administrative detention bundles of the 4th day model group and Durio Zibethinus murr shell extract and vitamin C administration group mouse stomach stress 18 hours, matched group is prohibited water fasting 18 hours, all take out the content of hepatic tissue with Griess chemical determination NO with the mice etherization.The result show the Durio Zibethinus murr shell extract can improve preferably restrain the liver NO disorder that stress cause (Fig. 4 for the Durio Zibethinus murr shell extract to restrain stress mice Mouse Liver NO influence).
Embodiment six, Durio Zibethinus murr shell extract to restrain stress cause the improvement that reduces of Mouse Liver ORAC (ORAC): experiment use that Guangdong Medical Lab Animal Center buys 6 age in week male mouse of kunming, 23 ± 2 ℃ of raising temperatures, lighting hours 12h hour/day (7:00-19:00) raises and is used for experiment after a week.Mice is divided into the blank group at random, restrains the Stress model group, positive drug control group (vitamin C 250mgkg
-1), low dosage Durio Zibethinus murr shell extract group (100mgkg
-1), middle dosage Durio Zibethinus murr shell extract group (300mgkg
-1), high dose Durio Zibethinus murr shell extract group (900mgkg
-1) wait the administration group, every group of 7 mices are divided into 6 groups.Matched group and contained load model group are all irritated stomach with the volume distilled water, every day 1 time, 30 minutes laggard administrative detention bundles of the 4th day model group and Durio Zibethinus murr shell extract and vitamin C administration group mouse stomach stress 18 hours, matched group is prohibited water fasting 18 hours, all are with the mice etherization, take out hepatic tissue, also improved the content of measuring ORAC with reference to people's methods such as Davalos.The result shows that the Durio Zibethinus murr shell extract can be replied preferably and restrains the hepatic tissue ORAC disorder that stress cause.(Fig. 5 be the Durio Zibethinus murr shell extract to restrain stress mice Mouse Liver ORAC influence, " * " expression and blank group be p＜0.05 relatively).
Claims (6)
1. Durio Zibethinus murr (Durio zibethinus Murr) shell or Durio Zibethinus murr shell extract are used to prepare and improve anti-stress effect and improve purposes in response to the medicine that swashs the various clinical symptoms that load causes, health food, food additive.
2. Durio Zibethinus murr (Durio zibethinus Murr) shell or Durio Zibethinus murr shell extract are used to prepare treatment or improve medicine, the health food of the various clinical symptoms that the oxidative stress load causes, the purposes of food additive.
3. Durio Zibethinus murr (Durio zibethinus Murr) shell or Durio Zibethinus murr shell extract are used to prepare the purposes of the medicine that improves the battle-weary various clinical symptoms that cause, health food, food additive.
4. according to claim 1,2 or 3 described purposes, it is characterized in that: described Durio Zibethinus murr (Durio zibethinus Murr) shell or Durio Zibethinus murr shell extract are that raw material can be used for improving and treating the various diseases of loading and causing in response to swashing, or improve the various clinical symptoms because of body peroxidating state cause.And can take for a long time, safely, the pharmaceuticals, food additive, health food and the constituent thereof that have no side effect.
5. according to claim 1,2 or 3 described purposes, it is characterized in that: described Durio Zibethinus murr shell or Durio Zibethinus murr shell comprehensively directly pulverization, ultrasound fragmentation or Durio Zibethinus murr shell or Durio Zibethinus murr shell extract be adopt hot water, pressurized heat to handle, the high temperature saturated vapor is handled, enzyme is handled or one or two or more kinds any mixed solvent of water and lower alcohols, polar solvent extracts micromolecular compound or macromole deposits such as recovery polysaccharide, esters, flavone, vitamin, volatile oil and protein.
6. according to claim 1,2 or 3 described purposes, it is characterized in that: the use amount of direct pulverization of described Durio Zibethinus murr shell or Durio Zibethinus murr shell extract extract is 0.001% to 100% weight ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100284681A CN101244095A (en) | 2007-06-07 | 2007-06-07 | New purpose of durio zibethinus L. shell extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100284681A CN101244095A (en) | 2007-06-07 | 2007-06-07 | New purpose of durio zibethinus L. shell extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101244095A true CN101244095A (en) | 2008-08-20 |
Family
ID=39944914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100284681A Pending CN101244095A (en) | 2007-06-07 | 2007-06-07 | New purpose of durio zibethinus L. shell extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101244095A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103405361A (en) * | 2013-07-24 | 2013-11-27 | 吴江市利达上光制品有限公司 | Perfume containing durian fruit shell |
| CN105816489A (en) * | 2016-04-19 | 2016-08-03 | 贺灿炎 | Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract |
| CN107320505A (en) * | 2017-07-13 | 2017-11-07 | 北京健旭康技术有限公司 | The effect of beneficial liver and its application of durian |
| CN110343189A (en) * | 2019-07-31 | 2019-10-18 | 河南大学 | Durian peel polysaccharide, extracting method and its application in terms of preparation adjusts intestinal flora and/or treats functional consitipation drug |
| CN110833568A (en) * | 2018-08-16 | 2020-02-25 | 百岳特生物科技（上海）有限公司 | Use of durian heart skin extract for improving muscle fatigue |
| CN111437287A (en) * | 2020-05-26 | 2020-07-24 | 河南大学 | Application of durian peel polysaccharide in preparation of immunopotentiating drugs |
| JP2020158437A (en) * | 2019-03-26 | 2020-10-01 | 宮崎 徹 | Composition for increasing aim of free form in blood |
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2007
- 2007-06-07 CN CNA2007100284681A patent/CN101244095A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103405361A (en) * | 2013-07-24 | 2013-11-27 | 吴江市利达上光制品有限公司 | Perfume containing durian fruit shell |
| CN103405361B (en) * | 2013-07-24 | 2015-10-28 | 广东爱车小屋实业发展股份有限公司 | A kind of perfume containing durian fruit shell |
| CN105816489A (en) * | 2016-04-19 | 2016-08-03 | 贺灿炎 | Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract |
| CN107320505A (en) * | 2017-07-13 | 2017-11-07 | 北京健旭康技术有限公司 | The effect of beneficial liver and its application of durian |
| CN110833568A (en) * | 2018-08-16 | 2020-02-25 | 百岳特生物科技（上海）有限公司 | Use of durian heart skin extract for improving muscle fatigue |
| CN110833568B (en) * | 2018-08-16 | 2021-11-02 | 百岳特生物科技（上海）有限公司 | Use of durian heart skin extract for improving muscle fatigue |
| JP2020158437A (en) * | 2019-03-26 | 2020-10-01 | 宮崎 徹 | Composition for increasing aim of free form in blood |
| JP7159093B2 (en) | 2019-03-26 | 2022-10-24 | 徹 宮崎 | Composition for increasing blood free body AIM |
| CN110343189A (en) * | 2019-07-31 | 2019-10-18 | 河南大学 | Durian peel polysaccharide, extracting method and its application in terms of preparation adjusts intestinal flora and/or treats functional consitipation drug |
| CN110343189B (en) * | 2019-07-31 | 2021-06-01 | 河南大学 | Durian peel polysaccharide, extraction method and application thereof in preparation of medicines for regulating intestinal flora and/or treating functional constipation |
| CN111437287A (en) * | 2020-05-26 | 2020-07-24 | 河南大学 | Application of durian peel polysaccharide in preparation of immunopotentiating drugs |
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