CN1005525B - Fast solid culture method for edible fungus mycelium - Google Patents
Fast solid culture method for edible fungus mycelium Download PDFInfo
- Publication number
- CN1005525B CN1005525B CN87105265.2A CN87105265A CN1005525B CN 1005525 B CN1005525 B CN 1005525B CN 87105265 A CN87105265 A CN 87105265A CN 1005525 B CN1005525 B CN 1005525B
- Authority
- CN
- China
- Prior art keywords
- mycelium
- culture
- solid culture
- mycelia
- food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000007787 solid Substances 0.000 title claims abstract description 15
- 241000233866 Fungi Species 0.000 title abstract description 5
- 238000012136 culture method Methods 0.000 title abstract description 4
- 239000000463 material Substances 0.000 claims abstract description 25
- 235000013305 food Nutrition 0.000 claims abstract description 19
- 238000012545 processing Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 13
- 240000007594 Oryza sativa Species 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 4
- 240000000588 Hericium erinaceus Species 0.000 claims description 4
- 235000007328 Hericium erinaceus Nutrition 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 240000006794 Volvariella volvacea Species 0.000 claims description 3
- 230000032696 parturition Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000001360 synchronised effect Effects 0.000 claims description 3
- 244000003416 Asparagus officinalis Species 0.000 claims description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 claims description 2
- 241000222336 Ganoderma Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 229930003270 Vitamin B Natural products 0.000 claims description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 235000019156 vitamin B Nutrition 0.000 claims description 2
- 239000011720 vitamin B Substances 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 7
- 235000013361 beverage Nutrition 0.000 abstract description 4
- 108010058643 Fungal Proteins Proteins 0.000 abstract 1
- 238000005273 aeration Methods 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000000790 scattering method Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 5
- 241000609240 Ambelania acida Species 0.000 description 4
- 241000123222 Hericium Species 0.000 description 4
- 239000010905 bagasse Substances 0.000 description 4
- 238000009738 saturating Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 244000060234 Gmelina philippensis Species 0.000 description 2
- 241000577951 Hydnum Species 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- -1 thalline polysaccharide Chemical class 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A fast solid culture method of edible fungus mycelium for food processing. The present invention belongs to a quick solid culture method of edible fungus mycelium, and is characterized by that it adopts cheap and easily-available agricultural and sideline products as solid culture base material, and adds the nutrient substance for promoting growth of mycelium, and adopts the scattering method to inoculate (granular strain or liquid strain), and adopts the aeration open culture in the late stage of growth of mycelium, and its whole growth period only needs about 12 days. The obtained mycelium and mycelium culture have no odor, and can be used as fungal protein for directly processing into food, or extracting for further processing into food, beverage or medicine.
Description
The invention belongs to edible mushroom mycelium quick solid cultural method, this mycelium and mycelium culture can directly be processed for food industry.
In recent years, be that the various health foods that raw material is made go on the market in succession with the edible mushroom, make special efficacy patent medicine and wait the research of medicine that bigger progress is also arranged with extraction is antitumor.Thereby edible mushroom has become the valuable source of food industry and the potential resource of pharmaceuticals industry.Because edible fungi growth is slower, natural resources are limited, and the artificial cultivation cycle is long and be subjected to the weather conditions restriction, and therefore, people seek one always and utilize the approach of edible mushroom mycelium aspect and further investigate, and also make certain gains.Majority is to adopt deep layer cultural methods such as cultivating mycelium in the fermentation tank in a large number (to see second national show of inventions, K class, numbering 17 at present, " deep layer of mushroom is cultivated " Yang Qingyao), or adopt bagasse etc. as solid culture base-material inoculated and cultured mycelium, extract then, use (seeing second national show of inventions, Q class, numbering 113 " hedgehog hydnum and Hericium Evimaccus slices ") for pharmacy.Though deep layer is cultivated advantages such as its cultivation cycle weak point is arranged, equipment investment is big, and the mycelium recovery rate is not high, and often causes being processed into the difficulty of food because of the bad peculiar smell of medium.The bagasse plate method has advantages such as cost is low, technology is simple, but this method cultivation cycle is long, and because the mycelium culture has bagasse, can not utilize its mycelium directly to make food, brings a lot of inconvenience to processing.
In order to improve mycelial yield, to shorten its growth cycle, mycelium capable of direct processing, free from extraneous odour is provided for food industry, the present invention proposes a kind of quick solid cultural method that can directly supply the edible mushroom mycelium of food industry processing.
The present invention adopts cheap starchiness agricultural byproducts (as rough rice, rice skin, corn, potato class etc.) to be matrix body, auxilliary plant nitrogenous source and the short nutriment of giving birth to of adding, make mycelia synchronous growth with disseminating method inoculation (granular bacteria strain or liquid spawn), simultaneously in the open cultivation of taking to ventilate of the after-stage of mycelial growth, it is vigorous to impel hyphal cell to breathe, and growth fast.Mycelia is covered with the whole cycle of saturating base-material only needs about 12 days, obtains fast 3～10 times of fruit body than the artificial culture method, than obtaining fast 2～4 times of mycelium with the conventional inoculated and cultured of bagasse.
Because host material of the present invention is the edibility material all, mycelium that obtains and mycelium culture are not with peculiar smell, and can be used as Fungal Protein and directly be processed into food.Owing to extract conveniently, soluble extract accounts for 65～75% of fresh mycelia culture weight, thereby can be applicable in the deep processing of food, beverage or medicine simultaneously.Show that by analysis these mycelium cultures have the nutritive value approaching with fruit body.
The used raw material of the present invention source is abundant, simple for process, applies easily, and has small investment, with short production cycle, advantages such as cost is low, wide application.
Each step of technical process of the present invention specifies as follows:
One, technological process.
Two, culture base-material prescription:
Rough rice (or corn, potato class) 50～94%
Corn broken 3～20%
Rice cortex (or wheat base bud) 2～25%
Calcium carbonate 0.05～0.1%
Vitamin B group 0.01～0.1g
Three, technology requires:
1. edible fungus species can be Hericium erinaceus, straw mushroom, Asparagus, flat mushroom, mushroom, Uricularia polytricha, glossy ganoderma etc.Require bacterial classification earlier through after purifying, the activation processing, adopt the granular bacteria strain culture medium inoculated to cultivate or the liquid fermentation and culture bacterial classification then.The of the right age bacterial classification that the bacterial classification that is adopted must be fresh, healthy and strong, vitality is strong and do not have living contaminants.
2. rough rice in the edible solid culture base-material and corn etc. should soak diel earlier and cook.Rough rice water absorption rate is advisable 35～40%.
3. the culture base-material preparation with 20 pounds of pressure sterilizations 30～60 minutes or tyndallization sterilization, is prepared inoculated and cultured by the sterile working requirement then after the bottling or the high-temperature resistance plastice bag of packing into have been filled in tampon afterwards.The also suitable use liquid spawn of this method.
4. adopt and disseminate the method inoculation, granular bacteria strain is evenly dispersed in the solid culture base-material as far as possible, inoculum concentration is 3～5%, covers with saturating culture base-material in a short time to impel mycelia.Because bacterial classification is evenly distributed in base-material and sowing point is intensive, so mycelia can obtain synchronous growth and diffusion is covered with in the whole culture base-material.
5. treat to have spread in the culture base-material when being covered with mycelia and forming the white hypha net, open tampon, maybe will cover with the culture base-material of mycelia and draw out the outer bacterium bed that is pressed into of bottle, ventilation open culturing.This moment, the hyphal cell breathing was vigorous, and growth rapidly.Early stage, cultivation temperature will be according to the characteristic and the suitable suitably low constant temperature culture of carrying out of control of temperature of giving birth to of mycelia of various different strains.Can carry out constant temperature culture with 18～24 ℃ as hericium mycelium, the straw mushroom mycelia can carry out constant temperature culture with 30～36 ℃.Treat that mycelia forms after the white hypha net in base-material, suitably improve cultivation temperature and air humidity and increase culturing room's brightness, impel the deeply long saturating base-material of mycelia and form scattered fruit body.
6. cultivation results: through the short-period cultivation in the left and right sides in 12 days, the overall broth material is all spread by dense white hypha and is covered with and deeply covers with saturating, (be mycelium culture surface and break the cross section, back into two with one's hands and all covered with) by the netted mycelia infiltration of dense white, culture base-material is because hyphostoma development breathing and growth metabolism consumption, weight obviously alleviates, and the mycelium culture has special bacterium fragrance.There is not any living contaminants.On the base-material surface or epidermis have simultaneously a large amount of overflow give birth to, scattered fruit bodys form, it is irregular that fruit body often is.The distinctive fragrant bacterium perfume (or spice) of tool, but without putrefaction.
7. the mycelium culture of passed examination after cultivating can directly be processed into food or crushed after being dried is used for food processing.Can also extract then, separate with the mycelium culture through hydrolysis or enzymatic liquefaction, can obtain the hypha extract of special edible mushroom fragrance, soluble extract accounts for 65～75% of fresh mycelia culture weight, recovery rate height.Extract is the light brown red liquid of clarification, can be applied to easily in the deep processing of health food, beverage or medicine.
Because host material of the present invention all is the edibility material, the mycelium of acquisition and mycelium culture are not with peculiar smell, do not have the non-edible material from soybeans for fear of food processing, directly are processed into food so can be used as Fungal Protein.Owing to extract conveniently, make edible mushroom mycelium be easier to be applied in the deep processing of food, beverage or medicine simultaneously.
Exemplary of the present invention is that the quick solid of hericium mycelium is cultivated, shows by analysis, and the mycelium culture of hericium erinaceus fruiting body and solid culture of the present invention, amino acid content is quite approaching.And, confirm that through long term studies and clinical testing the drug effect of Hericium erinaceus concentrates on the mycelium (sees second national show of inventions Q class numbering 113 " hedgehog hydnum and Hericium Evimaccus slice " data).
The edible mushroom mycelium that obtains with method of the present invention, contain rich in amino acid, protein, thalline polysaccharide, polypeptide, vitamin and mineral matter or the like, nutritious, some have in the edible mushroom mycelium of special drug effect can also extract multiple medicinal ingredients such as anticancer polysaccharide.
Claims (8)
1, a kind of edible mushroom mycelium rapid solid culture for food processing, it is characterized in that adopting the edibility material as culture base-material, after soaking, cook, pack into bottle or high-temperature resistance plastice bag, sterilization, the back makes mycelia synchronous growth with disseminating method inoculation (granular bacteria strain or liquid spawn), according to the suitable temperature constant temperature culture of giving birth to of the mycelia of different strain, treat to have spread in the base-material and be covered with mycelia and form after the white hypha net the open cultivation of employing ventilation.
2, a kind of edible mushroom mycelium rapid solid culture for food processing as claimed in claim 1 is characterized in that the prescription of used culture matrix material is:
Rough rice (corn, potato class) 50～94%
Corn broken 3～20%
Rice cortex (or wheat base bud) 2～25%
Calcium carbonate 0.05～0.1%
Vitamin B group 0.01～0.1g
3, a kind of edible mushroom mycelium rapid solid culture for food processing as claimed in claim 1 is characterized in that used inoculation bacterial classification can be Hericium erinaceus, straw mushroom, Asparagus, flat mushroom, mushroom, Uricularia polytricha, glossy ganoderma etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87105265.2A CN1005525B (en) | 1987-07-29 | 1987-07-29 | Fast solid culture method for edible fungus mycelium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87105265.2A CN1005525B (en) | 1987-07-29 | 1987-07-29 | Fast solid culture method for edible fungus mycelium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1031307A CN1031307A (en) | 1989-03-01 |
| CN1005525B true CN1005525B (en) | 1989-10-25 |
Family
ID=4815262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN87105265.2A Expired CN1005525B (en) | 1987-07-29 | 1987-07-29 | Fast solid culture method for edible fungus mycelium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1005525B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019491B (en) * | 2006-12-30 | 2010-08-18 | 张远富 | High yield veiled lady seed preparing and cultivating process |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069698C (en) * | 1995-06-28 | 2001-08-15 | 姚继迎 | Fungi hypha regenerating regulator |
| CN101874453B (en) * | 2010-04-28 | 2012-02-01 | 广东省农业科学院蔬菜研究所 | Culture method of snow white mushroom strains and sporocarps |
| CN102792851A (en) * | 2011-05-27 | 2012-11-28 | 绵阳市星光微生物研究所 | Preparation method and application of solid fomitopsis officinalis mycelia |
| CN102792849A (en) * | 2011-05-27 | 2012-11-28 | 绵阳市星光微生物研究所 | Preparation method and application of solid ganoderma lucidum mycelia |
| CN102792850A (en) * | 2011-05-27 | 2012-11-28 | 绵阳市星光微生物研究所 | Preparation method and application of solid coriolus versicolor mycelia |
| CN102511308B (en) * | 2011-12-12 | 2014-04-02 | 丽水市林业科学研究院 | Strain production method capable of preventing champignon virosis |
| CN102960711B (en) * | 2012-10-22 | 2013-10-30 | 李洁 | Natural nourishment and preparation method thereof |
| CN103053330A (en) * | 2013-01-25 | 2013-04-24 | 昆明欣尚科技开发有限公司 | Low-cost large-scale edible fungi liquid strain production process |
| CN104223047A (en) * | 2014-10-09 | 2014-12-24 | 哈尔滨艾克尔食品科技有限公司 | Straw mushroom chewable tablet and manufacturing method thereof |
| CN104480026B (en) * | 2014-12-25 | 2017-11-03 | 赵晶晶 | A kind of production method for being used to extract the Auricularia mycelium of Auricularia polysaccharide |
| CN104584859A (en) * | 2015-01-01 | 2015-05-06 | 曾建斌 | Production method for soft edible lucid ganoderma |
| CN106036265A (en) * | 2016-05-26 | 2016-10-26 | 明光市晶鑫饮料食品有限公司 | Cherry and red jujube beverage and making method thereof |
| CN106387589A (en) * | 2016-08-31 | 2017-02-15 | 内蒙古锦华生物科技有限公司金寨分公司 | Edible mushroom solid beverage for treating chronic pharyngitis |
| CN109006191A (en) * | 2018-10-19 | 2018-12-18 | 河北省微生物研究所 | The method that cultivation bacterium bag is prepared using mushroom liquid bacterial |
-
1987
- 1987-07-29 CN CN87105265.2A patent/CN1005525B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019491B (en) * | 2006-12-30 | 2010-08-18 | 张远富 | High yield veiled lady seed preparing and cultivating process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1031307A (en) | 1989-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101760478B (en) | Preparation method of radix puerariae red yeast rice | |
| KR100199920B1 (en) | The method of culturing cordyceps | |
| CN104770222A (en) | Method for inoculating stock spawn of edible fungi | |
| CN1005525B (en) | Fast solid culture method for edible fungus mycelium | |
| CN104798601A (en) | Cultivation method for lentinula edodes | |
| CN107593955A (en) | Ferment edible mushroom tea preparation process and fermentation edible mushroom tea | |
| CN103891523B (en) | A kind of Inonotus obliquus artificial cultivation method | |
| CN101100644A (en) | Method for culturing and processing edible fungus active function hypha powder | |
| CN105981581A (en) | Artificial culture method of cordyceps sobolifera | |
| CN103299824B (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
| CN104521550A (en) | Selenium-rich grifola frondosa cultivation method | |
| CN1248405A (en) | Method for producing pesticide-eating fungus floor | |
| CN108175040B (en) | Preparation method of millet oat antrodia camphorata mycoplasm health food | |
| CN105981584B (en) | A kind of cultural method of richness calcium cicada fungus | |
| CN102612986B (en) | Method for preparing ganoderma lucidum hypha block by using green corns | |
| CN114854604B (en) | Ganoderma lucidum, high-concentration oral liquid containing ganoderma lucidum and preparation method thereof | |
| CN104718990B (en) | The production method of a kind of composite fungi feed production and Edible Fungi unification | |
| KR20070008381A (en) | Method for culturing pleurotus eryngii including ginseng | |
| KR100557467B1 (en) | Process for feeding fish and livestock a liquid extract of mushroom mycelial culture and product thereof | |
| KR100752335B1 (en) | Method for culturing sangwhangletari mushroom using phellinus linteus mycelium and letari mushroom, and the mushroom culture mat for sangwhangletari mushroom | |
| CN114181837B (en) | New selenium-enriched Coulomb mushroom strain and artificial cultivation method thereof | |
| CN107997125A (en) | Edible mushroom mycelium and or edible mushroom mycelium powder manufacture craft and products thereof | |
| KR100204984B1 (en) | The method of culturing phellinus linteus | |
| KR20070048636A (en) | Method for culturing flammulina velutipes including ginseng saponin | |
| KR100762819B1 (en) | A method for effective fruiting body production of paecilomyces japonica using bombus terrestris and preparation of healthy food comprising the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| C13 | Decision | ||
| C14 | Grant of patent or utility model | ||
| C19 | Lapse of patent right due to non-payment of the annual fee |