CN102612986B - Method for preparing ganoderma lucidum hypha block by using green corns - Google Patents
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Abstract
The invention relates to a preparation method for a functional biological product, belongs to the technical field of biology, and particularly relates to a method for preparing a ganoderma lucidum hypha block by using green corns. The method is characterized by comprising the following steps of: preparing a culture medium by taking green corns as a main raw material, and taking a ganoderma lucidum liquid strain as a strain. The rich nutrition source and rich water contained in the fresh green corns are utilized and absorbed by the hypha for propagating, so that no nutrient solution is added, no mineral element is added, the product of mycelium is free from a heavy metal pollution source, and the preparation process for the culture medium is also convenient and quick; the fresh green corns are rich in nutrition, after being cultured, the ganoderma lucidum hypha is formed into another special combination with the corns, namely, the ganoderma lucidum hypha block, so that the nutrition, function and effect of the ganoderma lucidum hypha are more excellent; the common corns are also increased in value for hundred times in such a manner; and the ganoderma lucidum hypha block produced according to the method provided by the invention is excellent in market prospect and obvious in benefit.
Description
Technical field
The present invention relates to a kind of preparation method of functional living being goods, belong to biological technical field, be specifically related to a kind of method for preparing the Ganoderma lucidum mycelium piece with green corn.
Technical background
Glossy ganoderma is the treasure in motherland's pools of traditional Chinese medicine, have the good reputation of " celestial grass ".Pharmacology and clinical research at all times all proves, the effect that glossy ganoderma truly has and prevents and cures diseases, promotes longevity.The Compendium of Material Medica of the Shennong's Herbal of the Eastern Han Dynasty, the famous medical scholar's Li Shizhen (1518-1593 A.D.) of the Ming Dynasty, all the effect to glossy ganoderma has in detail and very sure record.Modern pharmacology and clinical practice have further confirmed the pharmacological action of glossy ganoderma, and confirm that GL-B is that glossy ganoderma is strengthened the body resistance to consolidate the constitution, strengthening by means of tonics, the main component of promoting longevity.Now, glossy ganoderma is formally recorded by NF as medicine, and it is again the new resource food of state approval simultaneously, has no side effect, can medicine-food two-purpose.
Scientific research shows, the pharmaceutical component of glossy ganoderma is very abundant, wherein effective ingredient can be divided into ten big classes, comprise GL-B, glossy ganoderma polypeptide, triterpenes, 16 seed amino acids (wherein containing seven kinds of essential amino acids), protein, steroid class, mannitol, coumarin glycosides, alkaloid, organic acid (the main fumaric acid that contains), and micro-Ge, P, Fe, Ca, Mn, Zn, etc.Glossy ganoderma has dual regulation to human body, and the kind of curing the disease relates to each systems such as cardiovascular and cerebrovascular, digestion, nerve, internal secretion, breathing, motion, and is especially very remarkable to the preventive and therapeutic effect of tumour, hepatic disease, insomnia and aging.
Along with the medical value of glossy ganoderma extensively is familiar with by people, the Wild ganoderma resource of modern remote, thickly forested mountains is also in continuous minimizing.At present, the ganoderma lucidum product on the market adopts artificial cultivation mostly, follow the natural variation that has by its product form of ganoderma lucidum fruitbody of artificial cultivation, but its curative effect is identical.But tame shortcoming is exactly inevitably will use agricultural chemicals in the course of cultivation and chemical method is cultivated bacterial classification, will use agricultural chemicals in the cultivation management process and control control of germ insect pest etc., brings substantial chemical contamination to ganoderma lucidum fruitbody.Yet chemical contamination is the source of human various diseases often.In addition, the biological transformation ratio of glossy ganoderma is all very low always, but the cycle of artificial cultivation glossy ganoderma is long, adds that production technology and management process are complicated and loaded down with trivial detailsly makes production cost cross height to cause benefit low.
Along with technical research is constantly goed deep into, obtained brand-new ganoderma lucidum product---ganoderma lucidum mycelium by biological submerged fermentation technology.Ganoderma lucidum mycelium is to be sprouted by Reishi sporule to form mycelia, and ganoderma lucidum fruitbody is constantly to decompose by mycelia, absorbs nutrient, and at suitable illumination, air, other condition such as temperature forms when cooperating.Launch conidial powder after the fruit body maturation, also just formed the history of life of glossy ganoderma, the needed constitute of ganoderma lucidum fruitbody has nutriment over half to derive from storage nutrient in the mycelium.Detect through relevant departments: ganoderma lucidum powder has the identical active ingredient of glossy ganoderma, and wherein thick polysaccharide and GL-B and Protein content are higher than natural glossy ganoderma far away.The mycelium product that develops be used for action effect such as insomnia forgetfulness, in poor health, neurasthenia and ganoderma spove powder similar; For treatment hypertension, coronary heart disease, diabetes, cancer, remarkable result is arranged, so ganoderma lucidum mycelium is by named as " good health and a long life food ".
Produce ganoderma lucidum mycelium with biological submerged fermentation technology, characteristics such as it is with short production cycle, technology is simple, production efficiency is high, stable are the main developing direction of manually cultivating ganoderma lucidum product.The accessory substance of the raw material main food that present production ganoderma lucidum mycelium is used or plant amylum, rice meal, corn flour, analysis for soybean powder etc., and to add a little mineral elements on the above-mentioned raw materials basis be carbon, nitrogenous source, after mycelium cultivates again by oven dry and be ground into powder.Mycelium can run off to some extent by drying its nutrient component of back.The function that has except product itself of the mycelium product that comes out with such explained hereafter, the effect, poor morphology, mouthfeel that its product forms are not good, even do not have any taste, can not attract much attention, so the product promotion poor effect in addition.
Summary of the invention
Purpose of the present invention is exactly to overcome the deficiencies in the prior art and brand-new a kind of method for preparing the Ganoderma lucidum mycelium piece with green corn is provided.Its feature comprises: with green corn be primary raw material prepare medium, with the ganoderma lucidum liquid bacterial classification as bacterial classification, inoculation method and inoculum concentration, mycelia piece cultural method and process.
Fresh green corn of the present invention is delicious flavour not only, and nutritious.In all staple foods, nutritive value and the health-care effect of corn are the highest.It is calcium, glutathione, vitamin, magnesium, selenium, vitamin E and fatty acid that corn contains 7 kinds " anatrophics ".After measured, per 100 gram corns can provide nearly 300 milligrams calcium, almost with dairy products in contained calcium similar.Studies show that: abundant calcium can play hypotensive effect.
Contain a large amount of natural VEs in the fresh and tender maize, the promotion cell division is arranged, delaying cell aging can also alleviate the symptom that arteriosclerosis and brain function decline occur.Being rich in lysine little in the dried corn in the bright corn, is the nutrient component of needed by human.In addition, the content of cellulose in the bright corn is many, and its amount is 6～8 times of polished rice, smart face.Practice confirms, often eats a little fresh corn people's health is benefited.
As the medium preparation ganoderma lucidum mycelium, the nutrient source that utilizes corn itself to be rich in absorbs breeding for mycelia, need not to add any mineral element with fresh green corn, ensures that the mycelium product does not have source of heavy metal pollution.The nutrition of fresh green corn own is just very abundant, and Ganoderma lucidum mycelium is cultivated combination---the corn Ganoderma lucidum mycelium piece that good back and corn form another characteristic, makes its nutrition, function and the effect can be more outstanding.Common corn also rises in value hundred times thus.
The present invention is achieved through the following technical solutions:
1, a kind ofly prepare the method for Ganoderma lucidum mycelium piece with green corn, its feature comprises: with green corn be primary raw material prepare medium, with the ganoderma lucidum liquid bacterial classification as bacterial classification, inoculation method and inoculum concentration, mycelia piece cultural method and process.
Described green corn is:
(1) quality standard: no insect pest, non agricultural chemical residuum, free from admixture, nothing are rotted, free from extraneous odour, color and luster normally reach fresh green corn grain;
(2) kind: one or more collocation wherein of conventional corn, sweet corn or waxy corn mix.
Described ganoderma lucidum liquid bacterial classification is selected and is required as follows:
(1) bacterial classification cultivation cycle: begin fermented and cultured to cultivation from mycelia and finish, its cultivation cycle is in 10 days, and the cycle that mycelia (ball) is cultivated surpasses 10 days and just begins to wear out, and influences strain quality;
(2) strain quality standard: even, energetic, good, the free of contamination liquid spawn of quality of mycelia (ball) size.
The preparation method of described medium is as follows:
(1) raw material is formed and proportioning: green corn grain 86%, corn flour 4%, plant amylum 3%, water 7%;
(2) batch mixing: add water with mixture furnishing pasty state after earlier corn flour, plant amylum being mixed, mix with the green corn grain again and be stirred into medium;
(3) charging: medium is sub-packed in the culture vessel, and charge weight is that the thickness of medium in culture vessel is 2～2.5cm;
(4) sterilization: the culture vessel that medium is housed is placed in the high-pressure bottle, through 1.5 kilograms/cm
2Pressure, temperature are to sterilize 30 minutes under 115～121 ℃ the condition and must produce and use medium.
Inoculation method and inoculum concentration when described ganoderma lucidum liquid bacterial classification inserts medium are as follows:
(1) inoculation method: with syringe imbitition bacterial classification according to quantity, the syringe marginal not is penetrated the limit and is moved during inoculation, and liquid spawn is expelled on the medium charge level equably under gnotobasis;
(2) insert kind of an amount: the weight according to medium inserts 10～12% liquid spawn.
Cultural method and the process of described mycelia piece are as follows:
(1) culture environment: the culture vessel that will connect kind moves on to culturing room's lucifuge and cultivates, and is that 25～30 ℃, relative moisture are to cultivate 5～6 days under 65～70% the environment in temperature, and medium covers with mycelia;
(2) finished product: when mycelia was covered with medium, medium was interweaved by Ganoderma lucidum mycelium and connects into piece, the Ganoderma lucidum mycelium piece cultivate finish and finished product.
The existing following advantage of the present invention:
1, simple, the easily enforcement of preparation technology proposed by the invention.
2, implement the used primary raw material of the present invention---fresh and tender maize, raw material is extensive, and cost is low.
3, in the implementation process of the present invention, with fresh and tender maize as the medium preparation ganoderma lucidum mycelium, the nutrient source that utilizes corn itself to be rich in absorbs and breeding for mycelia, need not to add any mineral element in the process, the formation that has ensured the mycelium product has not had source of heavy metal pollution, has possessed natural health, green health.The nutrition of fresh and tender maize own is just very abundant, Ganoderma lucidum mycelium is cultivated good combination---the corn glossy ganoderma silk piece that afterwards forms another characteristic with corn, make it not only can be more outstanding in nutrition, function and effect, and the formal beauty of product, novelty, characteristic, the good grade of mouthfeel will be the new lover that people consume.Common green corn is also rised in value tens of times thus.
4, the present invention is in the process of implementing, and the nutrient that has utilized green corn to possess does not need to add nutrient solution with abundant water content, and medium manufacturing process is convenient and swift.
5, by implementing the present invention, final products---the process for producing of Ganoderma lucidum mycelium piece has characteristics such as cultivation period is short, process for producing is simple, production cost of products is low, production efficiency of products is high and stablize.
6, by implementing the present invention, final products---Ganoderma lucidum mycelium piece, characteristics such as the formation of product is characteristic, novel, be of high nutritive value, mouthfeel is good also have special dietotherapy function such as prevent and cure diseases simultaneously; The product that forms can be cold and dressed with sauce, the method processing and eating such as fries, stew, and also can be used as the raw material of preparation mycelium, can, dilated food, functional characteristic snack etc. series of products.Produce the Ganoderma lucidum mycelium piece, market prospects are good, remarkable benefit.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
A kind ofly prepare the method for Ganoderma lucidum mycelium piece with green corn, specific implementation method is as follows:
1, select following green corn standby:
(1) quality standard: no insect pest, non agricultural chemical residuum, free from admixture, nothing are rotted, free from extraneous odour, color and luster normally reach fresh green corn grain;
(2) kind: one or more collocation wherein of conventional corn, sweet corn or waxy corn mix.
2, select following ganoderma lucidum liquid bacterial classification standby:
(1) bacterial classification cultivation cycle: begin fermented and cultured to cultivation from mycelia and finish, its cultivation cycle is in 10 days, and the cycle that mycelia (ball) is cultivated surpasses 10 days and just begins to wear out, and influences strain quality;
(2) strain quality standard: even, energetic, good, the free of contamination liquid spawn of quality of mycelia (ball) size.
3, preparation medium:
(1) raw material is formed and proportioning: green corn grain 86%, corn flour 4%, plant amylum 3%, water 7%;
(2) batch mixing: add water with mixture furnishing pasty state after earlier corn flour, plant amylum being mixed, mix with the green corn grain again and be stirred into medium;
(3) charging: medium is sub-packed in the culture vessel, and charge weight is that the thickness of medium in culture vessel is 2～2.5cm;
(4) sterilization: the culture vessel that medium is housed is placed in the high-pressure bottle, through 1.5 kilograms/cm
2Pressure, temperature are to sterilize 30 minutes under 115～121 ℃ the condition and must produce and use medium.
Inoculation method and inoculum concentration when 4, the ganoderma lucidum liquid bacterial classification inserts medium:
(1) inoculation method: with syringe imbitition bacterial classification according to quantity, the syringe marginal not is penetrated the limit and is moved during inoculation, and liquid spawn is expelled on the medium charge level equably under gnotobasis;
(2) insert kind of an amount: the weight according to medium inserts 10～12% liquid spawn.
5, the cultural method of mycelia piece and process:
(1) culture environment: the culture vessel that will connect kind moves on to culturing room's lucifuge and cultivates, and is that 25～30 ℃, relative moisture are to cultivate 5～6 days under 65～70% the environment in temperature, and medium covers with mycelia;
(2) finished product: when mycelia was covered with medium, medium was interweaved by Ganoderma lucidum mycelium and connects into piece, the Ganoderma lucidum mycelium piece cultivate finish and finished product.
Claims (1)
1. one kind prepares the method for Ganoderma lucidum mycelium piece with green corn, and its feature comprises: with green corn be primary raw material prepare medium, with the ganoderma lucidum liquid bacterial classification as bacterial classification, inoculation method and inoculum concentration, mycelia piece cultural method and process;
Described green corn is:
(1) quality standard: no insect pest, non agricultural chemical residuum, free from admixture, nothing are rotted, free from extraneous odour, color and luster normally reach fresh green corn grain;
(2) kind: one or more collocation wherein of conventional corn, sweet corn or waxy corn mix;
Described ganoderma lucidum liquid bacterial classification is selected and is required as follows:
(1) bacterial classification cultivation cycle: begin fermented and cultured to cultivation from mycelia and finish, its cultivation cycle is in 10 days, and the cycle that mycelia (ball) is cultivated surpasses 10 days and just begins to wear out, and influences strain quality;
(2) strain quality standard: even, energetic, good, the free of contamination liquid spawn of quality of mycelia (ball) size;
The preparation method of described medium is as follows:
(1) raw material is formed and proportioning: green corn grain 86%, corn flour 4%, plant amylum 3%, water 7%;
(2) batch mixing: add water with mixture furnishing pasty state after earlier corn flour, plant amylum being mixed, mix with the green corn grain again and be stirred into medium;
(3) charging: medium is sub-packed in the culture vessel, and charge weight is that the thickness of medium in culture vessel is 2～2.5cm;
(4) sterilization: the culture vessel that medium is housed is placed in the high-pressure bottle, through 1.5 kilograms/cm
2Pressure, temperature are to sterilize 30 minutes under 115～121 ℃ the condition and must produce and use medium;
Inoculation method and inoculum concentration when described ganoderma lucidum liquid bacterial classification inserts medium are as follows:
(1) inoculation method: with syringe imbitition bacterial classification according to quantity, the syringe marginal not is penetrated the limit and is moved during inoculation, and liquid spawn is expelled on the medium charge level equably under gnotobasis;
(2) insert kind of an amount: the weight according to medium inserts 10～12% liquid spawn;
Cultural method and the process of described mycelia piece are as follows:
(1) culture environment: the culture vessel that will connect kind moves on to culturing room's lucifuge and cultivates, and is that 25～30 ℃, relative moisture are to cultivate 5～6 days under 65～70% the environment in temperature, and medium covers with mycelia;
(2) finished product: when mycelia was covered with medium, medium was interweaved by Ganoderma lucidum mycelium and connects into piece, the Ganoderma lucidum mycelium piece cultivate finish and finished product.
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| CN103461005A (en) * | 2013-09-29 | 2013-12-25 | 凌爱秋 | Method for preparing cordyceps sinensis block by germinant wheat |
| CN106071486A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | A kind of sweet corn Ganoderma biological drink |
| CN110800555A (en) * | 2019-12-14 | 2020-02-18 | 辽宁省微生物科学研究院 | Method for preparing coprinus comatus cultivated species from corn kernels |
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| CN101703214A (en) * | 2009-11-09 | 2010-05-12 | 广东星河生物科技股份有限公司 | Lucid Ganoderma hypra powder or Lucid Ganoderma tea and double fermentation process |
| CN102172274A (en) * | 2011-03-09 | 2011-09-07 | 王永显 | Method for producing lucid ganoderma fermentation rice by utilizing solid state fermentation of rice |
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| CN101703214A (en) * | 2009-11-09 | 2010-05-12 | 广东星河生物科技股份有限公司 | Lucid Ganoderma hypra powder or Lucid Ganoderma tea and double fermentation process |
| CN102172274A (en) * | 2011-03-09 | 2011-09-07 | 王永显 | Method for producing lucid ganoderma fermentation rice by utilizing solid state fermentation of rice |
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