CN100347287C - 一种重组多形汉逊酵母菌及其构建方法与应用 - Google Patents
一种重组多形汉逊酵母菌及其构建方法与应用 Download PDFInfo
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- CN100347287C CN100347287C CNB2004100805172A CN200410080517A CN100347287C CN 100347287 C CN100347287 C CN 100347287C CN B2004100805172 A CNB2004100805172 A CN B2004100805172A CN 200410080517 A CN200410080517 A CN 200410080517A CN 100347287 C CN100347287 C CN 100347287C
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105727280A (zh) * | 2016-03-25 | 2016-07-06 | 汪和睦 | 基于表达HBsAg的热失活全重组汉逊酵母细胞的乙肝治疗疫苗 |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101440375B (zh) * | 2007-11-21 | 2013-01-30 | 中国科学院大连化学物理研究所 | 一种乳清酸核苷-5'-磷酸脱羧酶基因及其蛋白和应用 |
CN102234620A (zh) * | 2010-04-23 | 2011-11-09 | 北京生物制品研究所 | Dna序列、重组载体、单和双营养缺陷型多形汉逊酵母菌、及其制备方法 |
CN102226154B (zh) * | 2011-05-12 | 2013-02-06 | 中国科学院微生物研究所 | 具有双重筛选标记的多形汉逊酵母菌及其应用 |
CN103045492B (zh) * | 2012-12-31 | 2015-02-25 | 北京民海生物科技有限公司 | 一种汉逊酵母表达***及其构建方法和应用 |
CN103667274B (zh) * | 2013-12-10 | 2016-05-04 | 中国科学院微生物研究所 | 一种多形汉逊酵母遗传操作策略及其应用 |
CN105797151A (zh) * | 2016-03-25 | 2016-07-27 | 汪和睦 | 一种基于重组汉逊酵母的高剂量乙型肝炎疫苗 |
CN105727279B (zh) * | 2016-03-25 | 2019-01-15 | 汪和睦 | 基于表达HBsAg和HBcAg的热失活全重组汉逊酵母细胞的乙肝治疗疫苗 |
CN107418967B (zh) * | 2017-04-01 | 2020-09-11 | 中国科学院微生物研究所 | 尿嘧啶营养缺陷型多形汉逊酵母及其制备方法与应用 |
Citations (4)
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WO1998002536A2 (en) * | 1996-07-17 | 1998-01-22 | Zymogenetics, Inc. | Preparation of pichia methanolica auxotrophic mutants |
CN1237208A (zh) * | 1996-10-03 | 1999-12-01 | 基因工程和生物技术中心 | 用于产朊假丝酵母转化的方法和材料 |
CN1379811A (zh) * | 1999-08-19 | 2002-11-13 | 麒麟麦酒株式会社 | 新型酵母变异体以及制备含哺乳动物型糖链的糖蛋白的方法 |
CN1386134A (zh) * | 2000-05-25 | 2002-12-18 | 味之素株式会社 | 生产γ-谷氨酰半胱氨酸的方法 |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998002536A2 (en) * | 1996-07-17 | 1998-01-22 | Zymogenetics, Inc. | Preparation of pichia methanolica auxotrophic mutants |
CN1237208A (zh) * | 1996-10-03 | 1999-12-01 | 基因工程和生物技术中心 | 用于产朊假丝酵母转化的方法和材料 |
CN1379811A (zh) * | 1999-08-19 | 2002-11-13 | 麒麟麦酒株式会社 | 新型酵母变异体以及制备含哺乳动物型糖链的糖蛋白的方法 |
CN1386134A (zh) * | 2000-05-25 | 2002-12-18 | 味之素株式会社 | 生产γ-谷氨酰半胱氨酸的方法 |
Non-Patent Citations (1)
Title |
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扩增与序列分析 高建峰等.华中农业大学学报,第22卷第5期 2003 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105727280A (zh) * | 2016-03-25 | 2016-07-06 | 汪和睦 | 基于表达HBsAg的热失活全重组汉逊酵母细胞的乙肝治疗疫苗 |
CN105727280B (zh) * | 2016-03-25 | 2021-01-19 | 汪和睦 | 基于表达HBsAg的热失活全重组汉逊酵母细胞的乙肝治疗疫苗 |
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