CA2739501A1 - Procede de clarification a une densite de cellules superieure - Google Patents

Procede de clarification a une densite de cellules superieure Download PDF

Info

Publication number
CA2739501A1
CA2739501A1 CA2739501A CA2739501A CA2739501A1 CA 2739501 A1 CA2739501 A1 CA 2739501A1 CA 2739501 A CA2739501 A CA 2739501A CA 2739501 A CA2739501 A CA 2739501A CA 2739501 A1 CA2739501 A1 CA 2739501A1
Authority
CA
Canada
Prior art keywords
cell
cells
desired biological
supernatant layer
broth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2739501A
Other languages
English (en)
Inventor
Grigorius Zarbis-Papas-Toitsis
Michael C. Kuczewski
Emily Belcher Schirmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Percivia LLC
Original Assignee
Percivia LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Percivia LLC filed Critical Percivia LLC
Publication of CA2739501A1 publication Critical patent/CA2739501A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA2739501A 2008-10-17 2009-10-16 Procede de clarification a une densite de cellules superieure Abandoned CA2739501A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10632008P 2008-10-17 2008-10-17
US61/106,320 2008-10-17
EP08167506.8 2008-10-24
EP08167506 2008-10-24
PCT/EP2009/063565 WO2010043700A1 (fr) 2008-10-17 2009-10-16 Procédé de clarification à une densité de cellules supérieure

Publications (1)

Publication Number Publication Date
CA2739501A1 true CA2739501A1 (fr) 2010-04-22

Family

ID=40367660

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2739501A Abandoned CA2739501A1 (fr) 2008-10-17 2009-10-16 Procede de clarification a une densite de cellules superieure

Country Status (7)

Country Link
EP (1) EP2346891A1 (fr)
JP (1) JP2012505863A (fr)
KR (1) KR20110085980A (fr)
CN (1) CN102186875A (fr)
AU (1) AU2009305343A1 (fr)
CA (1) CA2739501A1 (fr)
WO (1) WO2010043700A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012030512A1 (fr) 2010-09-03 2012-03-08 Percivia Llc. Procédé de purification de protéine en flux
WO2015044418A1 (fr) 2013-09-30 2015-04-02 Crucell Holland B.V. Procédé de clarification de récolte de culture de cellules brutes à haute densité
JP2018517559A (ja) 2015-06-05 2018-07-05 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 吸着性バイオプロセス清澄化剤並びにその製造及び使用方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002325755A1 (en) * 2001-09-18 2003-04-01 Affinium Pharmaceuticals, Inc. Methods and apparatuses for purification
WO2007123961A2 (fr) * 2006-04-20 2007-11-01 Wyeth Procédés de purification pour isolation de virus purifié de la stomatite vésiculaire dans une culture cellulaire

Also Published As

Publication number Publication date
KR20110085980A (ko) 2011-07-27
CN102186875A (zh) 2011-09-14
JP2012505863A (ja) 2012-03-08
AU2009305343A1 (en) 2010-04-22
EP2346891A1 (fr) 2011-07-27
WO2010043700A1 (fr) 2010-04-22

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20131016