CA2362771A1 - Analyse genetique - Google Patents

Analyse genetique Download PDF

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Publication number
CA2362771A1
CA2362771A1 CA002362771A CA2362771A CA2362771A1 CA 2362771 A1 CA2362771 A1 CA 2362771A1 CA 002362771 A CA002362771 A CA 002362771A CA 2362771 A CA2362771 A CA 2362771A CA 2362771 A1 CA2362771 A1 CA 2362771A1
Authority
CA
Canada
Prior art keywords
dna
fragments
fragment
interest
restriction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002362771A
Other languages
English (en)
Inventor
Michael Alan Reeve
Nicholas Ian Workman
Luis Martin-Parras
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2362771A1 publication Critical patent/CA2362771A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne une technique d'analyse portant sur le génome complet. Cette technique, appelée déplétion en duplex à mésappariements parfaits inter-population, peut surmonter de nombreuses limitations des approches courantes basées sur les SNP et sur le déséquilibre de liaison au sein de populations isolées. La déplétion en duplex à mésappariements parfaits inter-population consiste à isoler un ou plusieurs fragments présentant des différences entre populations ou cellules <= touchées >= et <= non touchées >=. L'invention concerne une technique pratique - la matrice différentielle d'établissement de profils de sites de restriction terminaux (TRSPA) - pour l'analyse de tels fragments. L'invention concerne également un ADN de régulation interne totalement diagnostique permettant de déterminer sans ambiguïtés l'étendue et la nature exacte de toute digestion partielle pour la déplétion en duplex contenant des mésappariements parfaits inter-population et les restrictions TRSPA.
CA002362771A 1999-03-12 2000-03-10 Analyse genetique Abandoned CA2362771A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99301933.0 1999-03-12
EP99301933 1999-03-12
PCT/GB2000/000916 WO2000055364A2 (fr) 1999-03-12 2000-03-10 Analyse genetique

Publications (1)

Publication Number Publication Date
CA2362771A1 true CA2362771A1 (fr) 2000-09-21

Family

ID=8241266

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002362771A Abandoned CA2362771A1 (fr) 1999-03-12 2000-03-10 Analyse genetique

Country Status (5)

Country Link
EP (1) EP1173609A2 (fr)
JP (1) JP2002538837A (fr)
AU (1) AU3178200A (fr)
CA (1) CA2362771A1 (fr)
WO (1) WO2000055364A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7141371B2 (en) 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
CA2612145A1 (fr) * 2005-05-10 2006-11-16 Eric A. Johnson Procedes pour mapper les polymorphismes et microreseaux de polymorphismes
WO2011053987A1 (fr) * 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions et procédés de sélection et d'amplification de séquences d'acide nucléique ciblées
EP2769007B1 (fr) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions et procédés pour l'amplification et le séquençage directionnels d'acide nucléique
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013191775A2 (fr) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions et procédés pour la sélection négative de séquences d'acide nucléique indésirable
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2971130A4 (fr) 2013-03-15 2016-10-05 Nugen Technologies Inc Séquençage séquentiel
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
WO2015131107A1 (fr) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Séquençage au bisulfite de représentation réduite avec adaptateurs de diversité
CA2957633A1 (fr) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Mesures numeriques a partir de sequencage cible
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0226288A3 (fr) * 1985-10-09 1989-06-28 Collaborative Research Inc. Moyens et procédé de dosage de fibrose cystique à base d'une liaison génétique
US4771384A (en) * 1986-07-24 1988-09-13 Dnastar, Inc. System and method for fragmentation mapping
AU2318288A (en) * 1987-08-07 1989-03-09 Genelabs Incorporated Coincidence cloning method and library
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5316908A (en) * 1990-07-13 1994-05-31 Life Technologies, Inc. Size markers for electrophoretic analysis of DNA
WO1993017126A1 (fr) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Nouvelles configurations d'oligonucleotides et utilisation de ces configurations pour le tri, l'isolement, le sequençage et la manipulation des acides nucleiques
WO1993022457A1 (fr) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Criblage servant a detecter une variation genetique
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US5436142A (en) * 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
WO1995011971A1 (fr) * 1993-10-28 1995-05-04 Life Technologies, Inc. Echelle de reference d'acide nucleique pour l'estimation de la masse
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1997029211A1 (fr) * 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services VISUALISATION PAR RESTRICTION (RD-PCR) DES ARNm EXPRIMES DE MANIERE DIFFERENTIELLE

Also Published As

Publication number Publication date
WO2000055364A3 (fr) 2001-10-11
JP2002538837A (ja) 2002-11-19
AU3178200A (en) 2000-10-04
WO2000055364A2 (fr) 2000-09-21
EP1173609A2 (fr) 2002-01-23

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