CA2330252A1 - Procede de production d'hybrides d'acides nucleiques pour l'analyse des mutations - Google Patents

Procede de production d'hybrides d'acides nucleiques pour l'analyse des mutations Download PDF

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Publication number
CA2330252A1
CA2330252A1 CA002330252A CA2330252A CA2330252A1 CA 2330252 A1 CA2330252 A1 CA 2330252A1 CA 002330252 A CA002330252 A CA 002330252A CA 2330252 A CA2330252 A CA 2330252A CA 2330252 A1 CA2330252 A1 CA 2330252A1
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CA
Canada
Prior art keywords
sequences
primers
sense strand
complementary
linker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002330252A
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English (en)
Inventor
Andrew Wallace
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CENTRAL MANCHESTER HEALTHCARE NHS TRUST
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2330252A1 publication Critical patent/CA2330252A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention est un procédé de production d'une molécule d'ADN hybride permettant de réunir les séquences x¿1?, x¿2?.....x¿n? avec n égal à au moins 3 (cas des séquences donneuses) depuis divers emplacements pour aboutir à une molécule hybride destinée à l'analyse des mutations. Ce procédé de génération de la molécule hybride considérée comporte plusieurs opérations. La première opération consiste notamment (1) à réunir en un seul mélange de réaction, d'une part (a) les séquences x¿1?,x¿2?.......x¿n? et leurs séquences complémentaires x¿1?', x¿2?'.........x¿n?' à assembler pour donner la molécule hybride, et d'autre part, (b) pour chaque paire de séquences complémentaires définie en (a), une paire correspondante d'amorces pour amplification en chaîne par polymérase, chaque paire comportant une séquence d'amorçage et se présentant de façon que les amorces s'hybridant aux extrémités du 3' de chacune des deux séquences (x¿i?, x'¿(i+1)?) comportent des séquences de liaison spécifiquement complémentaires, et ce, pour i valant de 1 à (n-1). La seconde opération consiste (2) à réaliser un premier niveau de réaction d'amplification en chaîne par polymérase dans lequel, celles des amorces qui comportent des séquences de liaison, sont présentes avec des concentrations limitatives. La troisième et dernière opération consiste (3) à effectuer un second niveau de réaction d'amplification en chaîne par polymérase en utilisant une seule paire d'amorces dont l'une fournit l'extrémité du 5' du brin sens et l'autre fournit l'extrémité du 3' du brin antisens de la molécule hybride attendue.
CA002330252A 1998-06-12 1999-06-14 Procede de production d'hybrides d'acides nucleiques pour l'analyse des mutations Abandoned CA2330252A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9812674.1 1998-06-12
GBGB9812674.1A GB9812674D0 (en) 1998-06-12 1998-06-12 Nucleic acids
PCT/GB1999/001691 WO1999064624A2 (fr) 1998-06-12 1999-06-14 Acides nucleiques

Publications (1)

Publication Number Publication Date
CA2330252A1 true CA2330252A1 (fr) 1999-12-16

Family

ID=10833627

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002330252A Abandoned CA2330252A1 (fr) 1998-06-12 1999-06-14 Procede de production d'hybrides d'acides nucleiques pour l'analyse des mutations

Country Status (6)

Country Link
EP (1) EP1086250A2 (fr)
JP (1) JP2002517258A (fr)
AU (1) AU5377599A (fr)
CA (1) CA2330252A1 (fr)
GB (1) GB9812674D0 (fr)
WO (1) WO1999064624A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
EP2351853A1 (fr) * 2000-06-06 2011-08-03 Life Technologies Corporation Procédé et dispositif de multipléxage de réactions d'amplification
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
JP2006508662A (ja) 2002-12-04 2006-03-16 アプレラ コーポレイション ポリヌクレオチドの多重増幅
HU229967B1 (hu) 2011-12-20 2015-03-30 Kps Diagnosztika Zrt Eljárás töredezett nukleinsavak szekvenciájának meghatározására

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1992015678A1 (fr) * 1991-03-01 1992-09-17 Stratagene Molecules d'adn dicistroniques produites par reaction en chaine de polymerase et servant a produire des anticorps
ATE361377T1 (de) * 1997-09-29 2007-05-15 Hope City Leistungstarke verknüpfung von nukleinsäuresegmenten

Also Published As

Publication number Publication date
WO1999064624A3 (fr) 2000-09-14
GB9812674D0 (en) 1998-08-12
WO1999064624A2 (fr) 1999-12-16
EP1086250A2 (fr) 2001-03-28
JP2002517258A (ja) 2002-06-18
AU5377599A (en) 1999-12-30

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