BRPI0905853A8 - processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato. - Google Patents

processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato.

Info

Publication number
BRPI0905853A8
BRPI0905853A8 BRPI0905853A BRPI0905853A BRPI0905853A8 BR PI0905853 A8 BRPI0905853 A8 BR PI0905853A8 BR PI0905853 A BRPI0905853 A BR PI0905853A BR PI0905853 A BRPI0905853 A BR PI0905853A BR PI0905853 A8 BRPI0905853 A8 BR PI0905853A8
Authority
BR
Brazil
Prior art keywords
recombinant proteins
producing recombinant
phosphate uptake
fermentation media
improving phosphate
Prior art date
Application number
BRPI0905853A
Other languages
English (en)
Inventor
Goel Anuj
Kumar Bimal
Ramegowda Naveen Kumar Chittnalli
Iyer Harish
Kumar Garg Mayank
Babuappa Patale Mukesh
Tiwari Sanjay
Garg Saurabh
Joshi Sulekha
Original Assignee
Biocon Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocon Ltd filed Critical Biocon Ltd
Publication of BRPI0905853A2 publication Critical patent/BRPI0905853A2/pt
Publication of BRPI0905853A8 publication Critical patent/BRPI0905853A8/pt
Publication of BRPI0905853B1 publication Critical patent/BRPI0905853B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

meios de fermentação e processos destes. a presente invenção demonstra a utilidade de amidas de ácido carbônico como ureia ou seus derivados, carbamatos, carbodiimidas e tiocarbamidas como suplementos nitrogenosos em meios de fermentação para produção de proteínas recombinantes para atingir taxas de bioconversão elevadas e peptídeos semelhantes à insulina e análogos de insulina, exendina e enzimas como lipase usando sistemas de expressão fúngica induzíveis por metanol, como pichia.
BRPI0905853-2A 2008-02-06 2009-02-05 Processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato. BRPI0905853B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IN00310/CHE/2008 2008-02-06
IN310CH2008 2008-02-06
IN00086/CHE/2009 2009-01-12
IN86CH2009 2009-01-12
PCT/IN2009/000078 WO2009113099A2 (en) 2008-02-06 2009-02-05 Fermentation medias and processes thereof

Publications (3)

Publication Number Publication Date
BRPI0905853A2 BRPI0905853A2 (pt) 2015-06-30
BRPI0905853A8 true BRPI0905853A8 (pt) 2019-01-29
BRPI0905853B1 BRPI0905853B1 (pt) 2019-03-26

Family

ID=41065638

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0905853-2A BRPI0905853B1 (pt) 2008-02-06 2009-02-05 Processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato.

Country Status (12)

Country Link
US (2) US8927256B2 (pt)
EP (2) EP2565272A3 (pt)
JP (1) JP5587795B2 (pt)
KR (2) KR101492011B1 (pt)
CN (2) CN103642843B (pt)
BR (1) BRPI0905853B1 (pt)
IL (2) IL207402A (pt)
MX (2) MX2010008654A (pt)
MY (1) MY161108A (pt)
RU (3) RU2491345C2 (pt)
SG (1) SG188126A1 (pt)
WO (1) WO2009113099A2 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103642843B (zh) 2008-02-06 2017-03-01 拜康有限公司 含有尿素类氮源的发酵培养基及其用于生产次级代谢产物、酶和重组蛋白的用途
UA116217C2 (uk) 2012-10-09 2018-02-26 Санофі Пептидна сполука як подвійний агоніст рецепторів glp1-1 та глюкагону
EP2934569A1 (en) 2012-12-21 2015-10-28 Sanofi Exendin-4 derivatives
CN103911298B (zh) * 2012-12-31 2016-08-10 中粮营养健康研究院有限公司 培养基组合物和培养基及对菌株进行活菌计数的方法
EP3080152A1 (en) 2013-12-13 2016-10-19 Sanofi Non-acylated exendin-4 peptide analogues
TW201609795A (zh) 2013-12-13 2016-03-16 賽諾菲公司 作為雙重ｇｌｐ－１／ｇｉｐ受體促效劑的艾塞那肽－４（ｅｘｅｎｄｉｎ－４）胜肽類似物
WO2015086733A1 (en) 2013-12-13 2015-06-18 Sanofi Dual glp-1/glucagon receptor agonists
TW201609799A (zh) 2013-12-13 2016-03-16 賽諾菲公司 雙重ｇｌｐ－１／ｇｉｐ受體促效劑
TW201625670A (zh) 2014-04-07 2016-07-16 賽諾菲公司 衍生自ｅｘｅｎｄｉｎ－４之雙重ｇｌｐ－１／升糖素受體促效劑
TW201625669A (zh) 2014-04-07 2016-07-16 賽諾菲公司 衍生自艾塞那肽－４（Ｅｘｅｎｄｉｎ－４）之肽類雙重ＧＬＰ－１／升糖素受體促效劑
TW201625668A (zh) 2014-04-07 2016-07-16 賽諾菲公司 作為胜肽性雙重ｇｌｐ－１／昇糖素受體激動劑之艾塞那肽－４衍生物
US9932381B2 (en) 2014-06-18 2018-04-03 Sanofi Exendin-4 derivatives as selective glucagon receptor agonists
DE102014216863B4 (de) 2014-08-25 2019-08-01 Hansgrohe Se Kopfbrause
AR105319A1 (es) 2015-06-05 2017-09-27 Sanofi Sa Profármacos que comprenden un conjugado agonista dual de glp-1 / glucagón conector ácido hialurónico
TW201706291A (zh) 2015-07-10 2017-02-16 賽諾菲公司 作為選擇性肽雙重ｇｌｐ－１／升糖素受體促效劑之新毒蜥外泌肽（ｅｘｅｎｄｉｎ－４）衍生物
BR112018007314A2 (pt) 2015-10-20 2018-10-23 Buckman Laboratories Int Inc método para melhorar o crescimento da levedura para produção de bio-produtos, método para produção de bio-produtos fermentativos, composição nutriente de fermentação e mistura líquida
AU2017208935A1 (en) * 2016-01-23 2018-08-30 Biocon Limited Bio-analytical method for insulin analogues
CN107022591B (zh) * 2017-05-11 2018-06-12 宜昌东阳光长江药业股份有限公司 一种提高胰岛素及其类似物前体表达的毕赤酵母发酵方法
WO2018216978A1 (en) * 2017-05-22 2018-11-29 Cj Healthcare Corporation Cell culture method at low temperature applicable to mass production of protein
CN107988292B (zh) * 2018-01-18 2023-12-26 江苏江山聚源生物技术有限公司 提高重组人源胶原蛋白稳定性的发酵工艺
CN111411049B (zh) * 2020-04-15 2022-11-22 北京惠之衡生物科技有限公司 一种提高胰岛素前体表达的毕赤酵母的发酵培养基及发酵方法
CN111996136A (zh) * 2020-07-24 2020-11-27 北大方正集团有限公司 一种荧光假单孢杆菌发酵培养基、培养方法与应用
CN114507609B (zh) * 2020-12-22 2023-04-18 益海嘉里(连云港)生物科技有限公司 一种提高磷脂酶c蛋白产量的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4288554A (en) 1979-12-20 1981-09-08 Euteco Impianti S.P.A. Process for the cultivation of yeast cells
CN1017350B (zh) * 1987-08-12 1992-07-08 中国石油化工总公司 微生物发酵正烷烃生产长链α·ω-二羧酸的方法
RU2252258C2 (ru) * 1999-02-03 2005-05-20 Инститьют Фо Драг Ресеч Лтд. Микробный способ получения правастатина
WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
RU2141531C1 (ru) * 1999-05-26 1999-11-20 Российское акционерное общество открытого типа "Биопрепарат" Способ получения рекомбинантного инсулина человека
HUP9902352A1 (hu) * 1999-07-12 2000-09-28 Gyógyszerkutató Intézet Kft. Eljárás pravasztatin mikrobiológiai előállítására
KR100429925B1 (ko) * 2001-12-28 2004-05-03 씨제이 주식회사 5'-크산틸산을 생산하는 미생물
RU2208637C1 (ru) * 2002-04-16 2003-07-20 Институт биоорганической химии им. М.М. Шемякина и Ю.А. Овчинникова РАН Способ получения генно-инженерного инсулина человека
WO2004039996A1 (en) 2002-11-01 2004-05-13 Cadila Healthcare Limited Mthod for producing recombinant human interferon alpha 2b polypeptide in pichia pastoris
KR100470078B1 (ko) * 2003-06-12 2005-02-04 씨제이 주식회사 컴팩틴을 프라바스타틴으로 전환할 수 있는 스트렙토마이세스 종(Ｓｔｒｅｐｔｏｍｙｃｅｓ ｓｐ.)ＣＪＰＶ 975652 및 그를 이용한 프라바스타틴의 제조방법
US20050112737A1 (en) * 2003-11-20 2005-05-26 A. E. Staley Manufacturing Co. Lactic acid producing yeast
RU2278870C2 (ru) * 2004-08-30 2006-06-27 Закрытое Акционерное Общество "Биокад" Способ получения, выделения, очистки и стабилизации рекомбинантного гранулоцитарного колониестимулирующего фактора человека, пригодного для медицинского применения, и иммунобиологическое средство на его основе
WO2007005646A2 (en) 2005-07-01 2007-01-11 The University Of Florida Research Foundation, Inc. Recombinant host cells and media for ethanol production
KR20080019928A (ko) * 2006-08-29 2008-03-05 충청북도 질소원으로 요소를 이용한 마이코페놀산 대량 생산 방법
PL215829B1 (pl) * 2007-11-05 2014-01-31 Skotan Spolka Akcyjna Nowy szczep Yarrowia lipolytica oraz jego zastosowanie do przemyslowej utylizacji frakcji glicerolowej uzyskiwanej w produkcji biodiesla
CN103642843B (zh) * 2008-02-06 2017-03-01 拜康有限公司 含有尿素类氮源的发酵培养基及其用于生产次级代谢产物、酶和重组蛋白的用途

Also Published As

Publication number Publication date
EP2565272A2 (en) 2013-03-06
KR20100118592A (ko) 2010-11-05
RU2556120C2 (ru) 2015-07-10
BRPI0905853B1 (pt) 2019-03-26
RU2595380C2 (ru) 2016-08-27
BRPI0905853A2 (pt) 2015-06-30
RU2012145969A (ru) 2014-05-10
EP2565272A3 (en) 2013-07-03
IL207402A0 (en) 2010-12-30
US20100317056A1 (en) 2010-12-16
CN101983242A (zh) 2011-03-02
SG188126A1 (en) 2013-03-28
JP5587795B2 (ja) 2014-09-10
MX349413B (es) 2017-07-19
EP2247737A2 (en) 2010-11-10
RU2011153811A (ru) 2013-07-10
IL221379A0 (en) 2012-10-31
IL207402A (en) 2013-07-31
JP2011510677A (ja) 2011-04-07
WO2009113099A2 (en) 2009-09-17
KR20120066068A (ko) 2012-06-21
KR101492011B1 (ko) 2015-02-10
CN103642843A (zh) 2014-03-19
MY161108A (en) 2017-04-14
US9255285B2 (en) 2016-02-09
RU2491345C2 (ru) 2013-08-27
CN103642843B (zh) 2017-03-01
CN101983242B (zh) 2015-12-16
IL221379A (en) 2013-08-29
EP2247737A4 (en) 2012-03-14
RU2010137009A (ru) 2012-03-20
WO2009113099A3 (en) 2009-11-05
US20140141467A1 (en) 2014-05-22
US8927256B2 (en) 2015-01-06
EP2247737B1 (en) 2018-06-20
KR101283734B1 (ko) 2013-07-08
MX2010008654A (es) 2010-10-06

Similar Documents

Publication Publication Date Title
BRPI0905853A8 (pt) processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato.
BR112012033404A2 (pt) polipetídeo tendo ou auxiliando a atividade de degradação de material de carboidratos e uso dos mesmos.
MX2007009489A (es) Microorganismos que comprenden enzimas expresadas con baja actividad de eliminacion gamma.
PE20171798A1 (es) Formulacion que comprende n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada
NZ597001A (en) Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels
CA2877521C (en) Cells for producing recombinant iduronate-2-sulfatase
BR112012007854A2 (pt) metodos de produção de glicoproteínas em culturas de células de mamíferos usando glicocorticoides
BRPI0514694B8 (pt) processo de produção de etanercept em cultura de células de produção em larga escala
MX2013006234A (es) Metodo para la produccion de glicoproteinas recombinantes con semivida en circulacion incrementada en celulas de mamifero.
WO2011097381A3 (en) Engineered enzymes with methionine-gamma-lyase enzymes and pharmacological preparations therof
SG157346A1 (en) Improved purification of collagenases from clostridium histolyticum liquid culture
BR112012005788A2 (pt) farinhas produzidas com grãos miceliados com fungos
BR112013018385A2 (pt) microorganismo com produtividade de l-aminoácidos aumentada e processo de produção de l-aminoácidos usando o mesmo
BR112012033699A2 (pt) polipeptídeo tendo atividade beta-glicosidase e seus usos
BR112014006009A2 (pt) variantes de enzimas com propriedades aperfeiçoadas
TW200716138A (en) Stable active compound complex of salts of O-acetylsalicylic acid with basic amino acids and glycine
MX2020003798A (es) Metodos para la purificacion de arilsulfatasa a.
BRPI0519174A2 (pt) cepa recombinante de um microorganismo produtor de Ácido succÍnico, mÉtodo para produzir Ácido succÍnico, molÉcula de dna, plasmÍdeo de dna, e, cÉlula hospedeira
AR083354A1 (es) Polipeptidos variantes cbh i (celobiohidrolasas i) con reducida inhibicion de producto
BR112014003602A2 (pt) método para a produção de um sal de metionina
BRPI1013168A2 (pt) método para produção de açúcar, método para produção de etanol, método para produção de ácido láctico e método para produção de material de partida para sacarificação enzimática.
MX2014011614A (es) Fragmentos y proteinas nutritivas con fenilalanina baja o nula y metodos.
WO2010132341A3 (en) Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
WO2013016724A3 (en) Decarboxylase proteins with high keto-isovalerate decarboxylase activity
BR112014003841A2 (pt) estratégia de múltiplas cópias para produção em alto título e alta pureza de proteínas de múltiplas subunidades tal como anticorpos em micróbios transformados tal como pichia pastoris

Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 26/03/2019, OBSERVADAS AS CONDICOES LEGAIS.