BR9909459A - Protocolo de transformação de soja de eficiência melhorada - Google Patents

Protocolo de transformação de soja de eficiência melhorada

Info

Publication number
BR9909459A
BR9909459A BR9909459-2A BR9909459A BR9909459A BR 9909459 A BR9909459 A BR 9909459A BR 9909459 A BR9909459 A BR 9909459A BR 9909459 A BR9909459 A BR 9909459A
Authority
BR
Brazil
Prior art keywords
transformation
protocol
soybean
improved efficiency
processing protocol
Prior art date
Application number
BR9909459-2A
Other languages
English (en)
Other versions
BR9909459B1 (pt
Inventor
Brian J Martinelli
Lori J Julson
Maud A W Hinchee
Dannette Connor-Ward
Denis Mccabe
Carol A Emler
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Publication of BR9909459A publication Critical patent/BR9909459A/pt
Publication of BR9909459B1 publication Critical patent/BR9909459B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Patente de Invenção: <B>"PROTOCOLO DE TRANSFORMAçãO DE SOJA DE EFICIêNCIA MELHORADA"<D>. Um protocolo de transformação é descrito para a transformação mediada por partículas de soja. Este protocolo é com base na liberação de gene meristema de desenvolvimento de um embrião de soja. Antes da transformação dos protocolos com base no gene do caule médio ("meristema") a liberação em soja não dependeu da seleção devido à dificuldades em empregar agentes de seleção em tecido diferenciado em soja. Constatamos que uma cultura pós-bombardeio com a seleção de glifosato pode dramaticamente aumentar a eficiência de um tal protocolo de transformação.
BRPI9909459-2A 1998-04-06 1999-03-26 processo eficaz de obtenção de plantas de soja de linhagem germinal transformadas usando seleção de glifosfato. BR9909459B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/056,073 US5914451A (en) 1998-04-06 1998-04-06 Efficiency soybean transformation protocol
PCT/US1999/006672 WO1999051759A1 (en) 1998-04-06 1999-03-26 Particle mediated transformation of soybean with glyphosate selection

Publications (2)

Publication Number Publication Date
BR9909459A true BR9909459A (pt) 2000-12-12
BR9909459B1 BR9909459B1 (pt) 2011-05-31

Family

ID=22001985

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9909459-2A BR9909459B1 (pt) 1998-04-06 1999-03-26 processo eficaz de obtenção de plantas de soja de linhagem germinal transformadas usando seleção de glifosfato.

Country Status (14)

Country Link
US (1) US5914451A (pt)
EP (2) EP1728871B1 (pt)
JP (1) JP2002510499A (pt)
AT (2) ATE443150T1 (pt)
AU (1) AU3209599A (pt)
BR (1) BR9909459B1 (pt)
CA (1) CA2325331A1 (pt)
CY (1) CY1104999T1 (pt)
DE (2) DE69931619T2 (pt)
DK (1) DK1068341T3 (pt)
ES (1) ES2265684T3 (pt)
PT (1) PT1068341E (pt)
WO (1) WO1999051759A1 (pt)
ZA (1) ZA200005411B (pt)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000042207A2 (en) * 1999-01-14 2000-07-20 Monsanto Technology Llc Soybean transformation method
AU782918B2 (en) 1999-11-10 2005-09-08 University Of Washington Compositions and methods for modulation of plant cell division
US7687434B2 (en) * 2000-12-22 2010-03-30 Monsanto Technology, Llc Method of improving yield and vigor of plants
WO2002052025A2 (en) * 2000-12-22 2002-07-04 Monsanto Technology Llc Plant transformation process with selection and early identification of germline events
AU2002245266A1 (en) * 2001-01-16 2002-09-12 Monsanto Technology Llc Novel mutilple shoot proliferation and regeneration system for plants
AU2002362439A1 (en) * 2001-10-02 2003-04-14 Montague Farms, Inc. Identity preserved crop production
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
EP2216405A1 (en) 2002-05-03 2010-08-11 Monsanto Technology LLC Speed specific USP promoters for expressing genes in plants
MXPA05013188A (es) * 2003-06-06 2007-01-30 Arborgen Llc Composiciones y metodos para regular polisacaridos de una celula vegetal.
WO2005000471A1 (en) 2003-06-16 2005-01-06 Monsanto Technology Llc Method and apparatus for preparation of genetically transformable plant tissue
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
AR047598A1 (es) 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US20060041961A1 (en) 2004-03-25 2006-02-23 Abad Mark S Genes and uses for pant improvement
US20060075522A1 (en) 2004-07-31 2006-04-06 Jaclyn Cleveland Genes and uses for plant improvement
US7703238B2 (en) 2004-08-26 2010-04-27 Monsanto Technology Llc Methods of seed breeding using high throughput nondestructive seed sampling
US7832143B2 (en) 2004-08-26 2010-11-16 Monsanto Technology Llc High throughput methods for sampling seeds
US7799906B1 (en) 2004-09-22 2010-09-21 Arborgen, Llc Compositions and methods for modulating lignin of a plant
US7723570B2 (en) 2004-10-12 2010-05-25 Soymeds, Inc. Edible vaccines expressed in soybeans
US8314290B2 (en) 2004-12-21 2012-11-20 Monsanto Technology Llc Temporal regulation of gene expression by MicroRNAs
PL1827078T3 (pl) 2004-12-21 2014-07-31 Monsanto Technology Llc Rośliny transgeniczne z ulepszonymi cechami agronomicznymi
AU2005337132B2 (en) 2004-12-21 2011-01-20 Monsanto Technology, Llc Transgenic plants with enhanced agronomic traits
WO2006074400A2 (en) 2005-01-07 2006-07-13 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Method to trigger rna interference
EP2489726A3 (en) 2005-01-12 2012-11-28 Monsanto Technology LLC Genes and uses for plant improvement
WO2006138005A2 (en) 2005-05-10 2006-12-28 Monsanto Technology, Llc Genes and uses for plant improvement
AP2693A (en) 2005-05-27 2013-07-16 Monsanto Technology Llc Soybean event MON89788 and methods for detection thereof
CN101296613B (zh) 2005-07-29 2016-02-10 目标栽培公司 显性失活突变体krp蛋白保护活性细胞周期蛋白-cdk复合物不受野生型krp抑制
TW200730625A (en) 2005-08-24 2007-08-16 Pioneer Hi Bred Int Compositions providing tolerance to multiple herbicides and methods of use thereof
JP2009519719A (ja) 2005-12-15 2009-05-21 ターゲット・グロース・インコーポレーテッド 初期胚発生における発生及び／又は発生関連遺伝子のトランスジェニック過剰発現を通して増大された種子サイズ及び種子数
WO2007078280A2 (en) 2005-12-21 2007-07-12 Monsanto Technology, Llc Transgenic plants with enhanced agronomic traits
US20070283455A1 (en) * 2006-05-31 2007-12-06 Gray Dennis J Genetic Transformation of Grapevines
EP2048939A4 (en) 2006-08-17 2010-04-28 Monsanto Technology Llc TRANSGENIC PLANTS WITH ADVANCED AGRONOMIC CHARACTERISTICS
CL2007002532A1 (es) * 2006-08-31 2008-01-11 Monsanto Technology Llc Soc Organizada Bajo Las Leyes Del Estado De Delaware Procedimiento para identificar genes que confieren rasgos mejorados de una población de plantas.
WO2008027592A2 (en) 2006-08-31 2008-03-06 Monsanto Technology, Llc Phased small rnas
US8362317B2 (en) 2007-03-09 2013-01-29 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
PL2150113T3 (pl) 2007-04-25 2011-05-31 Syngenta Participations Ag Kompozycje fungicydowe
EP3567113A1 (en) 2007-06-06 2019-11-13 Monsanto Technology LLC Genes and uses for plant enhancement
EP2175714A4 (en) 2007-07-10 2011-01-26 Monsanto Technology Llc TRANSGENIC PLANTS WITH IMPROVED AGRONOMIC CHARACTERISTICS
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
CN102014628B (zh) 2008-02-05 2014-07-09 巴斯夫欧洲公司 植物健康组合物
MX2010008928A (es) 2008-02-15 2010-09-09 Monsanto Technology Llc Planta de soya y semilla que corresponde al evento transgenico mon87769 y metodos para deteccion del mismo.
EP2271760A2 (en) 2008-04-29 2011-01-12 Monsanto Technology LLC Genes and uses for plant enhancement
MX2010014147A (es) * 2008-06-20 2011-06-16 Univ Georgia Desarrollo de especies de cesped resistentes a herbicidas.
US20110214196A1 (en) * 2008-06-20 2011-09-01 University Of Georgia Research Foundation Development of herbicide-resistant grass species
US9040774B2 (en) 2008-07-01 2015-05-26 Monsanto Technology Llc Recombinant DNA constructs encoding ribonuclease cleavage blockers and methods for modulating expression of a target gene
EP3023499A1 (en) 2008-07-16 2016-05-25 Monsanto Technology LLC Methods and vectors for producing transgenic plants
US20110258735A1 (en) 2008-12-22 2011-10-20 Marie Coffin Genes and uses for plant enhancement
WO2010099431A2 (en) 2009-02-27 2010-09-02 Monsanto Technology Llc Hydroponic apparatus and methods of use
AU2010295864C1 (en) 2009-09-17 2015-04-30 Monsanto Technology Llc Soybean transgenic event MON 87708 and methods of use thereof
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
US20130047297A1 (en) 2010-03-08 2013-02-21 Robert D. Sammons Polynucleotide molecules for gene regulation in plants
US20130085066A1 (en) 2010-05-31 2013-04-04 Basf Se Method for Increasing the Health of a Plant
US8816153B2 (en) 2010-08-27 2014-08-26 Monsanto Technology Llc Recombinant DNA constructs employing site-specific recombination
US9701976B2 (en) 2011-06-30 2017-07-11 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof
MX343072B (es) 2011-09-13 2016-10-21 Monsanto Technology Llc Metodos y composiciones para controlar malezas.
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
CN103957697B (zh) 2011-09-13 2017-10-24 孟山都技术公司 用于杂草控制的方法和组合物
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
BR112014005979A8 (pt) 2011-09-13 2017-09-12 Monsanto Technology Llc Métodos e composições quimicas agricolas para controle de planta, método de redução de expressão de um gene ppg oxidase em uma planta, cassete de expressão microbiana, método para fazer um polinucleotídeo, método de identificação de polinucleotídeos úteis na modulação de expressão do gene ppg oxidase e mistura herbicida
BR112014005975A8 (pt) 2011-09-13 2017-09-12 Monsanto Technology Llc Método de controle de planta, método de redução de expressão de um gene pds em uma planta, cassete de expressão microbiana, método de fazer um polinucleotídeo, método de identificação de polinucleotídeos, e composições para controle de erva daninha
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
WO2013148330A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive fragments, proteins and methods
AR091143A1 (es) 2012-05-24 2015-01-14 Seeds Ltd Ab Composiciones y metodos para silenciar la expresion genetica
MX364070B (es) 2012-10-18 2019-04-10 Monsanto Technology Llc Métodos y composiciones para el control de plagas vegetales.
WO2014084884A1 (en) 2012-11-28 2014-06-05 Monsanto Technology Llc Transgenic plants with enhanced traits
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
EP2941487A2 (en) 2013-01-01 2015-11-11 A.B. Seeds Ltd. ISOLATED dsRNA MOLECULES AND METHODS OF USING SAME FOR SILENCING TARGET MOLECULES OF INTEREST
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
CA2905104A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Control of lolium species by topical application of herbicidal composition comprising dsrna
EP3604535A3 (en) 2013-03-13 2020-04-22 Monsanto Technology LLC Methods and compositions for weed control
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
CN105102474B (zh) * 2013-03-15 2019-11-12 拜耳作物科学有限合伙公司 组成型大豆启动子
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
BR112016000555B1 (pt) 2013-07-19 2022-12-27 Monsanto Technology Llc Método para controlar uma infestação da espécie de leptinotarsa em uma planta, composição inseticida e construção de dna recombinante
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
JP2016531559A (ja) * 2013-10-04 2016-10-13 ダウ アグロサイエンシィズ エルエルシー ダイズ形質転換法
US20160237447A1 (en) 2013-10-07 2016-08-18 Monsanto Technology Llc Transgenic Plants With Enhanced Traits
AU2014341879B2 (en) 2013-11-04 2020-07-23 Beeologics, Inc. Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
CN105979770B (zh) 2014-01-15 2019-07-05 孟山都技术公司 用于使用epsps多核苷酸的杂草控制的方法和组合物
EA201691886A1 (ru) 2014-03-20 2017-01-30 МОНСАНТО ТЕКНОЛОДЖИ ЭлЭлСи Трансгенный трансформант кукурузы mon 87419 и способы его применения
US11091770B2 (en) 2014-04-01 2021-08-17 Monsanto Technology Llc Compositions and methods for controlling insect pests
WO2015200223A1 (en) 2014-06-23 2015-12-30 Monsanto Technology Llc Compositions and methods for regulating gene expression via rna interference
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AU2015296700B2 (en) 2014-07-29 2021-10-21 Monsanto Technology Llc Compositions and methods for controlling insect pests
EP3256589B1 (en) 2015-01-22 2021-12-22 Monsanto Technology LLC Compositions and methods for controlling leptinotarsa
WO2016196738A1 (en) 2015-06-02 2016-12-08 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
US10655136B2 (en) 2015-06-03 2020-05-19 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
WO2017059341A1 (en) 2015-10-02 2017-04-06 Monsanto Technology Llc Recombinant maize b chromosome sequence and uses thereof
US11732268B2 (en) 2016-06-28 2023-08-22 Monsanto Technology Llc Methods and compositions for use in genome modification in plants
CN107099548B (zh) * 2017-05-09 2020-11-03 北京大北农生物技术有限公司 提高大豆转化效率的方法
US11377662B2 (en) 2018-01-10 2022-07-05 Wisconsin Alumni Research Foundation Agrobacterium-mediated and particle bombardment transformation method for cowpea and dry bean meristem explants
CN110343716A (zh) * 2018-04-04 2019-10-18 南京农业大学 一种利用绿色荧光蛋白的大豆遗传转化方法
CA3096518A1 (en) 2018-05-24 2019-11-28 Monsanto Technology Llc Genome editing in plants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE85360T1 (de) * 1985-08-07 1993-02-15 Monsanto Co Glyphosat resistente pflanzen.
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
ES2154628T3 (es) * 1989-11-17 2001-04-16 Monsanto Co Plantas de soja resistentes a inhibidores de glutamina sintetasa.
CA2036935A1 (en) * 1990-02-26 1991-08-27 Paul Christou Plant transformation process with early identification of germ line transformation events
DK0536330T3 (da) * 1990-06-25 2002-04-22 Monsanto Technology Llc Glyphosattolerante planter
US5633435A (en) * 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
CA2088661C (en) * 1990-08-31 2001-12-18 Gerard F. Barry Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5631152A (en) * 1994-10-26 1997-05-20 Monsanto Company Rapid and efficient regeneration of transgenic plants
AU4545497A (en) * 1997-10-07 1999-04-27 Embrapa-Empresa Brasileira De Pesquisa Agropecuaria A process for obtaining transgenic leguminous plants (leguminosae) containing exogenous dna

Also Published As

Publication number Publication date
JP2002510499A (ja) 2002-04-09
BR9909459B1 (pt) 2011-05-31
EP1728871B1 (en) 2009-09-16
CA2325331A1 (en) 1999-10-14
EP1728871A2 (en) 2006-12-06
CY1104999T1 (el) 2009-11-04
DE69931619T2 (de) 2007-05-10
PT1068341E (pt) 2006-08-31
US5914451A (en) 1999-06-22
DK1068341T3 (da) 2006-07-10
WO1999051759A1 (en) 1999-10-14
ES2265684T3 (es) 2007-02-16
EP1068341A1 (en) 2001-01-17
ATE328097T1 (de) 2006-06-15
AU3209599A (en) 1999-10-25
DE69941439D1 (de) 2009-10-29
DE69931619D1 (de) 2006-07-06
EP1728871A3 (en) 2006-12-13
ZA200005411B (en) 2002-03-27
ATE443150T1 (de) 2009-10-15
EP1068341B1 (en) 2006-05-31

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