BR112023004262A2 - Métodos para identificação do sítio de ligação usando espectrometria de massa de troca de hidrogênio - Google Patents
Métodos para identificação do sítio de ligação usando espectrometria de massa de troca de hidrogênioInfo
- Publication number
- BR112023004262A2 BR112023004262A2 BR112023004262A BR112023004262A BR112023004262A2 BR 112023004262 A2 BR112023004262 A2 BR 112023004262A2 BR 112023004262 A BR112023004262 A BR 112023004262A BR 112023004262 A BR112023004262 A BR 112023004262A BR 112023004262 A2 BR112023004262 A2 BR 112023004262A2
- Authority
- BR
- Brazil
- Prior art keywords
- binding site
- methods
- mass spectrometry
- hydrogen exchange
- exchange mass
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 229910052739 hydrogen Inorganic materials 0.000 title abstract 3
- 239000001257 hydrogen Substances 0.000 title abstract 3
- 238000004949 mass spectrometry Methods 0.000 title abstract 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical group [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 2
- 239000013315 hypercross-linked polymer Substances 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 230000000903 blocking effect Effects 0.000 abstract 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/475—Assays involving growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Peptides Or Proteins (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Hydrogen, Water And Hydrids (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
MÉTODOS PARA IDENTIFICAÇÃO DO SÍTIO DE LIGAÇÃO USANDO ESPECTROMETRIA DE MASSA DE TROCA DE HIDROGÊNIO. A presente invenção refere-se a métodos para identificar um sítio de ligação entre um produto farmacêutico de proteína e uma proteína de célula hospedeira (HCP) usando espectrometria de massa de troca de hidrogênio. O presente pedido também fornece métodos para modificar produtos farmacêuticos de proteína para eliminar a clivagem ou modificação por HCPs. Além disso, o presente pedido fornece métodos para bloquear o sítio de ligação identificado em produtos farmacêuticos de proteína para eliminar a clivagem ou modificação por HCPs.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063077220P | 2020-09-11 | 2020-09-11 | |
PCT/US2021/049975 WO2022056336A1 (en) | 2020-09-11 | 2021-09-11 | Methods for binding site identification using hydrogen exchange mass spectrometry |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112023004262A2 true BR112023004262A2 (pt) | 2023-04-04 |
Family
ID=78333247
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112023004262A BR112023004262A2 (pt) | 2020-09-11 | 2021-09-11 | Métodos para identificação do sítio de ligação usando espectrometria de massa de troca de hidrogênio |
Country Status (11)
Country | Link |
---|---|
US (1) | US20220082571A1 (pt) |
EP (1) | EP4211255A1 (pt) |
JP (1) | JP2023542858A (pt) |
KR (1) | KR20230066025A (pt) |
CN (1) | CN116490777A (pt) |
AU (1) | AU2021342274A1 (pt) |
BR (1) | BR112023004262A2 (pt) |
CA (1) | CA3194955A1 (pt) |
IL (1) | IL301234A (pt) |
MX (1) | MX2023002924A (pt) |
WO (1) | WO2022056336A1 (pt) |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4604345B2 (ja) * | 2000-12-21 | 2011-01-05 | 住友化学株式会社 | タンパク質のn末端のアミノ酸配列決定方法 |
US20020182649A1 (en) * | 2001-02-01 | 2002-12-05 | Ciphergen Biosystems, Inc. | Methods for protein identification, characterization and sequencing by tandem mass spectrometry |
US20050233406A1 (en) * | 2002-04-10 | 2005-10-20 | Woods Virgil L Jr | Methods for high resolution identification of solvent accessible amide hydrogens in polypeptides and for characterization of polypeptide structure |
WO2006020498A2 (en) * | 2004-08-11 | 2006-02-23 | The Cleveland Clinic Foundation | Therapeutic agents and methods for cardiovascular disease |
US20100212030A1 (en) * | 2007-10-19 | 2010-08-19 | Pronota N.V. | Use of n-terminal and c-terminal proteomics technology to enhance protein therapeutics and diagnostics |
ES2535963T3 (es) * | 2009-02-09 | 2015-05-19 | Roche Glycart Ag | Análisis del patrón de glicosilación de inmunoglobulina |
ES2567276T3 (es) * | 2011-05-12 | 2016-04-21 | Genentech, Inc. | Método de LC-MS/MS de monitoreo de múltiples reacciones para detectar anticuerpos terapéuticos en muestras de animales usando péptidos de cambio de marco |
WO2015120036A1 (en) * | 2014-02-04 | 2015-08-13 | University Of Virginia Patent Foundation | Compositions and methods for analysis of protein sequences and post-translational modifications |
WO2017097706A1 (en) * | 2015-12-09 | 2017-06-15 | F. Hoffmann-La Roche Ag | Direct affinity measurement of human igg1 binding multimeric antigens |
JP6788010B2 (ja) * | 2015-12-09 | 2020-11-18 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | インビボ相互作用様式を決定するための方法 |
CN109891244A (zh) * | 2016-08-12 | 2019-06-14 | 隆扎有限公司 | 对宿主细胞蛋白质的蛋白质组分析 |
-
2021
- 2021-09-11 WO PCT/US2021/049975 patent/WO2022056336A1/en active Application Filing
- 2021-09-11 JP JP2023516058A patent/JP2023542858A/ja active Pending
- 2021-09-11 CA CA3194955A patent/CA3194955A1/en active Pending
- 2021-09-11 KR KR1020237011422A patent/KR20230066025A/ko unknown
- 2021-09-11 AU AU2021342274A patent/AU2021342274A1/en active Pending
- 2021-09-11 CN CN202180062105.8A patent/CN116490777A/zh active Pending
- 2021-09-11 IL IL301234A patent/IL301234A/en unknown
- 2021-09-11 US US17/472,605 patent/US20220082571A1/en active Pending
- 2021-09-11 MX MX2023002924A patent/MX2023002924A/es unknown
- 2021-09-11 EP EP21798168.7A patent/EP4211255A1/en active Pending
- 2021-09-11 BR BR112023004262A patent/BR112023004262A2/pt unknown
Also Published As
Publication number | Publication date |
---|---|
EP4211255A1 (en) | 2023-07-19 |
MX2023002924A (es) | 2023-04-10 |
KR20230066025A (ko) | 2023-05-12 |
CA3194955A1 (en) | 2022-03-17 |
WO2022056336A1 (en) | 2022-03-17 |
AU2021342274A1 (en) | 2023-04-27 |
CN116490777A (zh) | 2023-07-25 |
US20220082571A1 (en) | 2022-03-17 |
JP2023542858A (ja) | 2023-10-12 |
IL301234A (en) | 2023-05-01 |
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