BR112019011093A2 - cepas de levedura aperfeiçoadas para a produção de etanol - Google Patents

cepas de levedura aperfeiçoadas para a produção de etanol

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Publication number
BR112019011093A2
BR112019011093A2 BR112019011093A BR112019011093A BR112019011093A2 BR 112019011093 A2 BR112019011093 A2 BR 112019011093A2 BR 112019011093 A BR112019011093 A BR 112019011093A BR 112019011093 A BR112019011093 A BR 112019011093A BR 112019011093 A2 BR112019011093 A2 BR 112019011093A2
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BR
Brazil
Prior art keywords
enzyme
ethanol production
yeast strains
cell
improved yeast
Prior art date
Application number
BR112019011093A
Other languages
English (en)
Inventor
Jeroen Adriaan Van Maris Antonius
Papapetridis Ioannis
Thomas Pronk Jacobus
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112019011093A2 publication Critical patent/BR112019011093A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01094—Glycerol-3-phosphate dehydrogenase (NAD(P)+)(1.1.1.94)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção refere-se a uma célula recombinante, de preferência a uma célula de levedura recombinante compreendendo: a. um gene que codifica uma enzima tendo atividade de desidrogenase de glicerol-3-fosfato, em que a dita enzima tem uma dependência de cofator para pelo menos nadp+ e/ou para nadph; b) um gene que codifica uma enzima tendo pelo menos atividade de desidrogenase de acetaldeído de acetilação dependente de nad+ (ec 1.2.1.10); e c) uma mutação ou rompimento em pelo menos um gene selecionado do grupo de gpd1 e gpd2. a dita célula é adequada para a produção de etanol, tem uma produção de glicerol reduzida a alto rendimento de etanol.
BR112019011093A 2016-12-20 2017-12-18 cepas de levedura aperfeiçoadas para a produção de etanol BR112019011093A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16205338 2016-12-20
EP17155525 2017-02-10
PCT/EP2017/083242 WO2018114758A1 (en) 2016-12-20 2017-12-18 Improved yeast strains for ethanol production

Publications (1)

Publication Number Publication Date
BR112019011093A2 true BR112019011093A2 (pt) 2019-10-22

Family

ID=60972198

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019011093A BR112019011093A2 (pt) 2016-12-20 2017-12-18 cepas de levedura aperfeiçoadas para a produção de etanol

Country Status (9)

Country Link
US (1) US10913928B2 (pt)
EP (1) EP3559246B1 (pt)
CN (1) CN110088288B (pt)
BR (1) BR112019011093A2 (pt)
CA (1) CA3045739A1 (pt)
DK (1) DK3559246T3 (pt)
ES (1) ES2924108T3 (pt)
PL (1) PL3559246T3 (pt)
WO (1) WO2018114758A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022261003A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0948628B1 (en) * 1996-11-13 2010-06-23 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
US7253001B2 (en) 2002-03-19 2007-08-07 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of Saccharomyces cerevisiae
EP2173881B1 (en) 2007-07-23 2016-09-28 DSM IP Assets B.V. Acetyl-coa producing enzymes in yeast
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
CN104608496B (zh) 2012-01-13 2017-01-04 精工爱普生株式会社 盒、印刷材料供应***、印刷装置、液体容纳容器、印刷***、以及端子连接结构
US9988650B2 (en) * 2012-11-20 2018-06-05 Lallenmend Hungary Liquidity Management LLC Electron consuming ethanol production pathway to displace glycerol formation in S. cerevisiae
WO2014151318A2 (en) * 2013-03-15 2014-09-25 Mascoma Corporation Methods for regulating nitrogen metabolism during the production of ethanol from corn by metabolically engineered yeast strains
MX2016001881A (es) * 2013-08-15 2016-08-03 Lallemand Hungary Liquidity Man Llc Metodos para la mejora de rendimiento y produccion de producto en un microorganismo a traves del reciclaje de glicerol.
AR097479A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células convertidoras de glicerol y ácido acético con un transporte de glicerol mejorado
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
WO2015041940A2 (en) * 2013-09-19 2015-03-26 Shell Oil Company Novel yeast strains
EP3122876B1 (en) 2014-03-28 2020-11-25 Danisco US Inc. Altered host cell pathway for improved ethanol production
US9955950B2 (en) 2014-07-30 2018-05-01 General Electric Company Systems and methods for steering multiple ultrasound beams
CN107636144B (zh) * 2015-05-22 2021-02-19 帝斯曼知识产权资产管理有限公司 消耗乙酸盐/酯的酵母细胞
PL3359655T3 (pl) 2015-10-06 2020-05-18 Dsm Ip Assets B.V. Komórka eukariotyczna ze zwiększonym wytwarzaniem produktu fermentacji

Also Published As

Publication number Publication date
DK3559246T3 (da) 2022-09-05
EP3559246B1 (en) 2022-06-15
CA3045739A1 (en) 2018-06-28
US10913928B2 (en) 2021-02-09
EP3559246A1 (en) 2019-10-30
WO2018114758A1 (en) 2018-06-28
US20200095536A1 (en) 2020-03-26
ES2924108T3 (es) 2022-10-04
PL3559246T3 (pl) 2022-09-05
CN110088288B (zh) 2023-08-29
CN110088288A (zh) 2019-08-02

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