BR112019006744A2 - micro-organismos engenheirados para produção de produtos químicos de commodity e biomassa celular - Google Patents

micro-organismos engenheirados para produção de produtos químicos de commodity e biomassa celular

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Publication number
BR112019006744A2
BR112019006744A2 BR112019006744A BR112019006744A BR112019006744A2 BR 112019006744 A2 BR112019006744 A2 BR 112019006744A2 BR 112019006744 A BR112019006744 A BR 112019006744A BR 112019006744 A BR112019006744 A BR 112019006744A BR 112019006744 A2 BR112019006744 A2 BR 112019006744A2
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BR
Brazil
Prior art keywords
present disclosure
host cell
udh
disclosure provides
genetically modified
Prior art date
Application number
BR112019006744A
Other languages
English (en)
Inventor
E Dueber John
Joseph Protzko Ryan
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of BR112019006744A2 publication Critical patent/BR112019006744A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01088—Hydroxymethylglutaryl-CoA reductase (1.1.1.88)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01203—Uronate dehydrogenase (1.1.1.203)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01004—Aldehyde dehydrogenase (NADP+) (1.2.1.4)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente divulgação fornece métodos para produzir produtos de commodity, os métodos envolvendo cultivar uma célula hospedeira que é geneticamente modificada para produzir uma uronato desidrogenase (udh) que converte um ácido de açúcar em sua correspondente 1,5-aldonolactona que usa nadp+ ou nad+ como um cofator e que produz nadph ou nadh, respectivamente, quando a célula hospedeira coexpressa uma redutase endógena ou uma heteróloga que utiliza a nadph ou nadh para gerar o produto de commodity ou um precursor do mesmo. a presente divulgação fornece um método para produzir produtos a jusante de glicerol e piruvato em uma célula hospedeira microbiana geneticamente modificada, o método envolvendo cultivar uma célula hospedeira microbiana geneticamente modificada da presente divulgação em um meio de cultura compreendendo ácido d-galacturônico. a presente divulgação fornece polipeptídeos udh variantes que utilizam nadp+, ácidos nucleicos codificando os polipeptídeos udh variantes e células hospedeiras geneticamente modificadas com os ácido nucleicos.
BR112019006744A 2016-10-03 2017-10-02 micro-organismos engenheirados para produção de produtos químicos de commodity e biomassa celular BR112019006744A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662403523P 2016-10-03 2016-10-03
PCT/US2017/054759 WO2018067458A1 (en) 2016-10-03 2017-10-02 Engineered microorganisms for production of commodity chemicals and cellular biomass

Publications (1)

Publication Number Publication Date
BR112019006744A2 true BR112019006744A2 (pt) 2019-06-25

Family

ID=61831847

Family Applications (1)

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BR112019006744A BR112019006744A2 (pt) 2016-10-03 2017-10-02 micro-organismos engenheirados para produção de produtos químicos de commodity e biomassa celular

Country Status (4)

Country Link
US (1) US11332723B2 (pt)
EP (1) EP3519567A4 (pt)
BR (1) BR112019006744A2 (pt)
WO (1) WO2018067458A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3460067A1 (en) * 2017-09-20 2019-03-27 Technische Universität München Process for enzymatic oxidation of linear carbohydrates
JP7370587B2 (ja) * 2018-10-30 2023-10-30 ＧｒｅｅｎＥａｒｔｈＩｎｓｔｉｔｕｔｅ株式会社 有機化合物の製造方法およびコリネ型細菌
US11091783B2 (en) * 2019-05-21 2021-08-17 The United States Of America As Represented By The Secretary Of The Army Use of galacturonate and or galacturonate polymers in conjunction with carbohydrates to control metabolic state of organisms
WO2021041932A2 (en) * 2019-08-28 2021-03-04 The Regents Of The University Of California Genetically engineered microbes and biosynthetic methods
US20240052387A1 (en) * 2019-10-08 2024-02-15 Johann Wokfgang Goethe-Universitat Frankfurt Am Main Variants of gala reductase and their uses

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Publication number Priority date Publication date Assignee Title
US20060177916A1 (en) 2005-02-07 2006-08-10 Renewable Spirits, Llc Ethanol production from citrus processing waste
FI20055263A0 (fi) 2005-05-30 2005-05-30 Valtion Teknillinen Sokerihappojen mikrobinen muuttaminen ja siihen soveltuvia välineitä
JP5200241B2 (ja) * 2006-11-17 2013-06-05 国立大学法人 東京大学 Ｎ末端に非天然骨格をもつポリペプチドの翻訳合成とその応用
EP2274417B1 (en) * 2008-04-04 2013-07-24 Massachusetts Institute of Technology Cellular production of glucaric acid
WO2010030711A2 (en) 2008-09-10 2010-03-18 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol
FI20086236A0 (fi) * 2008-12-23 2008-12-23 Valtion Teknillinen Heksuronihapon konversio heksarihapoksi
US20130055471A1 (en) * 2009-12-15 2013-02-28 Edwin Henricus Antonius HOLMAN Transgenic Ozone-Resistant Plants
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
BR112014003546A2 (pt) * 2011-08-15 2017-03-14 Toyota Motor Co Ltd método para a produção de alcano e micro-organismo recombinante capazes de sintetizar alcano
KR102043381B1 (ko) 2011-08-19 2019-11-11 게노마티카 인코포레이티드 2,4-펜타디에노에이트, 부타디엔, 프로필렌, 1,3-부탄디올 및 관련 알코올을 생합성하기 위한 미생물과 방법
US9322023B2 (en) * 2011-10-06 2016-04-26 Cornell University Constructs and methods for the assembly of biological pathways
WO2013115959A1 (en) * 2012-02-02 2013-08-08 Bio Architecture Lab, Inc. Methods and compositions for growth of yeast on alginate
SG11201604591YA (en) 2013-12-04 2016-07-28 Agency Science Tech & Res Chemical process to convert mucic acid to adipic acid

Also Published As

Publication number Publication date
WO2018067458A1 (en) 2018-04-12
EP3519567A4 (en) 2020-06-03
EP3519567A1 (en) 2019-08-07
US11332723B2 (en) 2022-05-17
US20190218527A1 (en) 2019-07-18

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Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements