BR112017009152A2 - métodos de produção de proteínas de duas cadeias em bactérias - Google Patents
métodos de produção de proteínas de duas cadeias em bactériasInfo
- Publication number
- BR112017009152A2 BR112017009152A2 BR112017009152A BR112017009152A BR112017009152A2 BR 112017009152 A2 BR112017009152 A2 BR 112017009152A2 BR 112017009152 A BR112017009152 A BR 112017009152A BR 112017009152 A BR112017009152 A BR 112017009152A BR 112017009152 A2 BR112017009152 A2 BR 112017009152A2
- Authority
- BR
- Brazil
- Prior art keywords
- bacteria
- production methods
- protein production
- chain protein
- methods
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 230000014616 translation Effects 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 102000040739 Secretory proteins Human genes 0.000 abstract 1
- 108091058545 Secretory proteins Proteins 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
Abstract
são fornecidos aqui métodos de produção de um polipeptídeo que contém duas cadeias, tal como um anticorpo, incluindo uma cadeia leve e uma cadeia pesada. em particular, são fornecidos métodos para a produção de proteínas secretoras heterólogas em bactérias, através da utilização de vetores de expressão e processos de cultura otimizados.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462075792P | 2014-11-05 | 2014-11-05 | |
US201562207882P | 2015-08-20 | 2015-08-20 | |
PCT/US2015/059339 WO2016073791A1 (en) | 2014-11-05 | 2015-11-05 | Methods of producing two chain proteins in bacteria |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112017009152A2 true BR112017009152A2 (pt) | 2018-03-06 |
Family
ID=54754738
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017009152A BR112017009152A2 (pt) | 2014-11-05 | 2015-11-05 | métodos de produção de proteínas de duas cadeias em bactérias |
Country Status (13)
Country | Link |
---|---|
US (2) | US10112994B2 (pt) |
EP (1) | EP3215526A1 (pt) |
JP (3) | JP6875276B2 (pt) |
KR (1) | KR20170075793A (pt) |
CN (1) | CN108064308B (pt) |
AU (1) | AU2015342961B2 (pt) |
BR (1) | BR112017009152A2 (pt) |
CA (1) | CA2966558C (pt) |
HK (1) | HK1255201A1 (pt) |
IL (1) | IL252027B (pt) |
MX (1) | MX2017005930A (pt) |
RU (1) | RU2705274C2 (pt) |
WO (1) | WO2016073791A1 (pt) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2966558C (en) | 2014-11-05 | 2024-03-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
RU2020141422A (ru) | 2014-11-05 | 2021-01-13 | Дженентек, Инк. | Способы получения двуцепочечных белков в бактериях |
AU2019375413A1 (en) | 2018-11-05 | 2021-05-27 | Genentech, Inc. | Methods of producing two chain proteins in prokaryotic host cells |
EP3914615A1 (en) * | 2019-01-23 | 2021-12-01 | F. Hoffmann-La Roche AG | Methods of producing multimeric proteins in eukaryotic host cells |
Family Cites Families (87)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE266710C (pt) | ||||
FR2413974A1 (fr) | 1978-01-06 | 1979-08-03 | David Bernard | Sechoir pour feuilles imprimees par serigraphie |
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
DD266710A3 (de) | 1983-06-06 | 1989-04-12 | Ve Forschungszentrum Biotechnologie | Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase |
US4676980A (en) | 1985-09-23 | 1987-06-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target specific cross-linked heteroantibodies |
US6548640B1 (en) | 1986-03-27 | 2003-04-15 | Btg International Limited | Altered antibodies |
IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
EP0307434B2 (en) | 1987-03-18 | 1998-07-29 | Scotgen Biopharmaceuticals, Inc. | Altered antibodies |
CA1338518C (en) | 1987-09-23 | 1996-08-13 | Joyce M. Zarling | Antibody heteroconjugates for the killing of hiv-infected cells |
GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
KR0184860B1 (ko) | 1988-11-11 | 1999-04-01 | 메디칼 리써어치 카운실 | 단일영역 리간드와 이를 포함하는 수용체 및 이들의 제조방법과 이용(법) |
DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
DE69029036T2 (de) | 1989-06-29 | 1997-05-22 | Medarex Inc | Bispezifische reagenzien für die aids-therapie |
US6150584A (en) | 1990-01-12 | 2000-11-21 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
DE69120146T2 (de) | 1990-01-12 | 1996-12-12 | Cell Genesys Inc | Erzeugung xenogener antikörper |
US6075181A (en) | 1990-01-12 | 2000-06-13 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
ES2246502T3 (es) | 1990-08-29 | 2006-02-16 | Genpharm International, Inc. | Animales no humanos transgenicos capaces de producir anticuerpos heterologos. |
US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
ES2113940T3 (es) | 1990-12-03 | 1998-05-16 | Genentech Inc | Metodo de enriquecimiento para variantes de proteinas con propiedades de union alteradas. |
US5571894A (en) | 1991-02-05 | 1996-11-05 | Ciba-Geigy Corporation | Recombinant antibodies specific for a growth factor receptor |
EP0586505A1 (en) | 1991-05-14 | 1994-03-16 | Repligen Corporation | Heteroconjugate antibodies for treatment of hiv infection |
ES2206447T3 (es) | 1991-06-14 | 2004-05-16 | Genentech, Inc. | Anticuerpo humanizado para heregulina. |
GB9114948D0 (en) | 1991-07-11 | 1991-08-28 | Pfizer Ltd | Process for preparing sertraline intermediates |
US7018809B1 (en) | 1991-09-19 | 2006-03-28 | Genentech, Inc. | Expression of functional antibody fragments |
US5587458A (en) | 1991-10-07 | 1996-12-24 | Aronex Pharmaceuticals, Inc. | Anti-erbB-2 antibodies, combinations thereof, and therapeutic and diagnostic uses thereof |
WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
AU675929B2 (en) | 1992-02-06 | 1997-02-27 | Curis, Inc. | Biosynthetic binding protein for cancer marker |
ATE149570T1 (de) | 1992-08-17 | 1997-03-15 | Genentech Inc | Bispezifische immunoadhesine |
JPH08511420A (ja) | 1993-06-16 | 1996-12-03 | セルテック・セラピューテイクス・リミテッド | 抗 体 |
US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
US5731168A (en) | 1995-03-01 | 1998-03-24 | Genentech, Inc. | Method for making heteromultimeric polypeptides |
US5840523A (en) | 1995-03-01 | 1998-11-24 | Genetech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
US5869046A (en) | 1995-04-14 | 1999-02-09 | Genentech, Inc. | Altered polypeptides with increased half-life |
ES2304786T3 (es) | 1995-04-27 | 2008-10-16 | Amgen Fremont Inc. | Anticuerpos anti-il-8 humanos, derivados a partir de xenoratones inmunizados. |
EP0823941A4 (en) | 1995-04-28 | 2001-09-19 | Abgenix Inc | HUMAN ANTIBODIES DERIVED FROM IMMUNIZED XENO MOUSES |
DK1500329T3 (da) | 1996-12-03 | 2012-07-09 | Amgen Fremont Inc | Humane antistoffer, der specifikt binder TNF-alfa |
US6610833B1 (en) | 1997-11-24 | 2003-08-26 | The Institute For Human Genetics And Biochemistry | Monoclonal human natural antibodies |
ATE531812T1 (de) | 1997-12-05 | 2011-11-15 | Scripps Research Inst | Humanisierung von nager-antikörpern |
US6194551B1 (en) | 1998-04-02 | 2001-02-27 | Genentech, Inc. | Polypeptide variants |
ATE375365T1 (de) | 1998-04-02 | 2007-10-15 | Genentech Inc | Antikörper varianten und fragmente davon |
HUP0104865A3 (en) | 1999-01-15 | 2004-07-28 | Genentech Inc | Polypeptide variants with altered effector function |
US6737056B1 (en) | 1999-01-15 | 2004-05-18 | Genentech, Inc. | Polypeptide variants with altered effector function |
JP2002541073A (ja) | 1999-03-17 | 2002-12-03 | メディカル リサーチ カウンシル | Igドメイン含有ポリペプチドの分子リフォールディング方法 |
GB9911569D0 (en) * | 1999-05-18 | 1999-07-21 | Oxford Biomedica Ltd | Antibodies |
AU784983B2 (en) | 1999-12-15 | 2006-08-17 | Genentech Inc. | Shotgun scanning, a combinatorial method for mapping functional protein epitopes |
ES2324645T3 (es) * | 2000-11-03 | 2009-08-12 | Genentech, Inc. | Cambios de tasa metabolica en fermentaciones que expresen proteinas recombinantes. |
WO2002061090A2 (en) * | 2000-12-14 | 2002-08-08 | Genentech, Inc. | Prokaryotically produced antibodies and uses thereof |
ATE382053T1 (de) | 2001-08-27 | 2008-01-15 | Genentech Inc | System zur antikörperexpression und- synthese |
ATE290020T1 (de) | 2001-08-31 | 2005-03-15 | Avidex Ltd | Löslicher t zell rezeptor |
EP1513879B1 (en) | 2002-06-03 | 2018-08-22 | Genentech, Inc. | Synthetic antibody phage libraries |
AU2003267999B2 (en) * | 2002-07-19 | 2010-03-11 | Abbvie Biotechnology Ltd | Treatment of TNFalpha related disorders |
US7361740B2 (en) | 2002-10-15 | 2008-04-22 | Pdl Biopharma, Inc. | Alteration of FcRn binding affinities or serum half-lives of antibodies by mutagenesis |
DE60332957D1 (de) | 2002-12-16 | 2010-07-22 | Genentech Inc | Immunoglobulinvarianten und deren verwendungen |
AU2004205631A1 (en) | 2003-01-16 | 2004-08-05 | Genentech, Inc. | Synthetic antibody phage libraries |
US7635472B2 (en) | 2003-05-31 | 2009-12-22 | Micromet Ag | Pharmaceutical compositions comprising bispecific anti-cd3, anti-cd19 antibody constructs for the treatment of b-cell related disorders |
SI1641822T1 (sl) | 2003-07-08 | 2013-08-30 | Genentech, Inc. | IL-17 A/F heterologni polipeptidi in njihove terapevtske uporabe |
DK1691833T3 (da) | 2003-11-28 | 2010-05-03 | Micromet Ag | Præparater der omfatter polypeptider |
US7235641B2 (en) | 2003-12-22 | 2007-06-26 | Micromet Ag | Bispecific antibodies |
JP4943161B2 (ja) | 2003-12-23 | 2012-05-30 | ジェネンテック, インコーポレイテッド | 新規抗il13モノクローナル抗体での癌の処置 |
JP4501451B2 (ja) | 2004-02-18 | 2010-07-14 | 株式会社豊田自動織機 | 塗料組成物、塗料組成物を用いた透明性保護膜の製造方法および透明性保護膜を有する有機ガラス |
MXPA06011199A (es) | 2004-03-31 | 2007-04-16 | Genentech Inc | Anticuerpos anti-tgf-beta humanizados. |
US7785903B2 (en) | 2004-04-09 | 2010-08-31 | Genentech, Inc. | Variable domain library and uses |
CA2885854C (en) | 2004-04-13 | 2017-02-21 | F. Hoffmann-La Roche Ag | Anti-p-selectin antibodies |
CA2577082A1 (en) | 2004-09-02 | 2006-03-16 | Genentech, Inc. | Heteromultimeric molecules |
TWI380996B (zh) | 2004-09-17 | 2013-01-01 | Hoffmann La Roche | 抗ox40l抗體 |
AU2006301492B2 (en) | 2005-10-11 | 2011-06-09 | Amgen Research (Munich) Gmbh | Compositions comprising cross-species-specific antibodies and uses thereof |
ES2577292T3 (es) | 2005-11-07 | 2016-07-14 | Genentech, Inc. | Polipéptidos de unión con secuencias hipervariables de VH/VL diversificadas y consenso |
US20070237764A1 (en) | 2005-12-02 | 2007-10-11 | Genentech, Inc. | Binding polypeptides with restricted diversity sequences |
CA2651567A1 (en) | 2006-05-09 | 2007-11-22 | Genentech, Inc. | Binding polypeptides with optimized scaffolds |
LT2520590T (lt) | 2007-04-03 | 2018-09-10 | Amgen Research (Munich) Gmbh | Susijusioms rūšims specifinis rišantis domenas |
CN100592373C (zh) | 2007-05-25 | 2010-02-24 | 群康科技(深圳)有限公司 | 液晶显示面板驱动装置及其驱动方法 |
KR20110025649A (ko) * | 2008-05-05 | 2011-03-10 | 노비뮨 에스 에이 | 항-il-17a/il-17f 교차-반응성 항체 및 그의 사용 방법 |
RU2011148913A (ru) | 2009-05-01 | 2013-06-10 | Эбботт Лэборетриз | Иммуноглобулин с двумя вариабельными доменами и его применение |
RU2585488C2 (ru) | 2009-11-05 | 2016-05-27 | Дженентек, Инк. | Способы и композиция для секреции гетерологичных полипептидов |
ES2617777T5 (es) | 2010-04-23 | 2022-10-13 | Hoffmann La Roche | Producción de proteínas heteromultiméricas |
WO2012106615A1 (en) | 2011-02-03 | 2012-08-09 | Xoma Technology Ltd. | Methods and materials for enhancing functional protein expression in bacteria |
US9158890B2 (en) | 2011-07-27 | 2015-10-13 | At&T Mobility Ii Llc | Mobile applications and methods for conveying performance information of a cardiac pacemaker |
EP3418306B1 (en) * | 2011-10-11 | 2023-12-06 | F. Hoffmann-La Roche AG | Improved assembly of bispecific antibodies |
WO2014165771A2 (en) * | 2013-04-05 | 2014-10-09 | Genentech, Inc. | Anti-il-4 antibodies and bispecific antibodies and uses thereof |
SG11201508642WA (en) | 2013-04-19 | 2015-11-27 | Sutro Biopharma Inc | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
BR112016018980A2 (pt) * | 2014-02-21 | 2017-10-10 | Genentech Inc | método de tratamento de um distúrbio, anticorpo multiespecífico, ácido nucleico isolado, célula hospedeira, métodos de produzir um anticorpo, de produção de uma metade de anticorpo ou de um anticorpo multiespecífico e de produção de um anticorpo multiespecífico, imunoconjugado e formulação farmacêutica |
CA2966558C (en) | 2014-11-05 | 2024-03-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
RU2020141422A (ru) * | 2014-11-05 | 2021-01-13 | Дженентек, Инк. | Способы получения двуцепочечных белков в бактериях |
-
2015
- 2015-11-05 CA CA2966558A patent/CA2966558C/en active Active
- 2015-11-05 WO PCT/US2015/059339 patent/WO2016073791A1/en active Application Filing
- 2015-11-05 CN CN201580059973.5A patent/CN108064308B/zh active Active
- 2015-11-05 EP EP15802233.5A patent/EP3215526A1/en active Pending
- 2015-11-05 BR BR112017009152A patent/BR112017009152A2/pt active Search and Examination
- 2015-11-05 RU RU2017119403A patent/RU2705274C2/ru active
- 2015-11-05 AU AU2015342961A patent/AU2015342961B2/en active Active
- 2015-11-05 MX MX2017005930A patent/MX2017005930A/es unknown
- 2015-11-05 KR KR1020177014850A patent/KR20170075793A/ko not_active Application Discontinuation
- 2015-11-05 US US14/934,100 patent/US10112994B2/en active Active
- 2015-11-05 JP JP2017523894A patent/JP6875276B2/ja active Active
-
2017
- 2017-04-30 IL IL252027A patent/IL252027B/en unknown
-
2018
- 2018-09-21 US US16/138,180 patent/US11299539B2/en active Active
- 2018-11-08 HK HK18114324.8A patent/HK1255201A1/zh unknown
-
2021
- 2021-04-20 JP JP2021070858A patent/JP2021119777A/ja not_active Withdrawn
-
2023
- 2023-03-14 JP JP2023039366A patent/JP2023088935A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
JP2018500008A (ja) | 2018-01-11 |
HK1255201A1 (zh) | 2019-08-09 |
RU2017119403A3 (pt) | 2019-03-27 |
CN108064308B (zh) | 2023-06-09 |
WO2016073791A1 (en) | 2016-05-12 |
IL252027A0 (en) | 2017-06-29 |
CA2966558A1 (en) | 2016-05-12 |
CA2966558C (en) | 2024-03-12 |
US11299539B2 (en) | 2022-04-12 |
EP3215526A1 (en) | 2017-09-13 |
MX2017005930A (es) | 2017-06-30 |
RU2017119403A (ru) | 2018-12-05 |
JP2023088935A (ja) | 2023-06-27 |
AU2015342961B2 (en) | 2021-08-12 |
IL252027B (en) | 2021-10-31 |
RU2705274C2 (ru) | 2019-11-06 |
AU2015342961A1 (en) | 2017-06-15 |
CN108064308A (zh) | 2018-05-22 |
JP6875276B2 (ja) | 2021-05-19 |
KR20170075793A (ko) | 2017-07-03 |
US20190112368A1 (en) | 2019-04-18 |
US20160159898A1 (en) | 2016-06-09 |
US10112994B2 (en) | 2018-10-30 |
JP2021119777A (ja) | 2021-08-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CY1121217T1 (el) | Μεθοδοι για συγκομιδη καλλιεργειων κυτταρου θηλαστικων | |
MX2022013540A (es) | Constructos de expresion y metodos para dise?ar geneticamente levadura metilotrofica. | |
CO2019006485A2 (es) | Anticuerpos y metodos de su utilizacion | |
MX2017006127A (es) | Metodos y composiciones para produccion de proteina de clara de huevo. | |
CO2017001023A2 (es) | Composiciones de portador-anticuerpo y métodos para realizarlas y utilizarlas | |
MA39819A (fr) | Méthodes et compositions d'immunomodulation | |
IL249828A0 (en) | Limbal stem cells of cultured mammals, methods of their creation and uses thereof | |
PE20210107A1 (es) | Anticuerpos anti-cd3 y metodos de uso | |
AR101845A1 (es) | Anticuerpos anti-her2 e inmunoconjugados | |
MX2016016886A (es) | Anticuerpos anti-axl. | |
BR112017009262A2 (pt) | métodos de produção de proteínas de duas cadeias em bactérias | |
BR112016016916A2 (pt) | método de tratamento de angiodema hereditário (hae) | |
EA201791275A1 (ru) | Способы получения ctp-модифицированных полипептидов длительного действия | |
MX2020003508A (es) | Un factor de secrecion de proteinas con alta eficacia secretora y un vector de expresion que lo comprende. | |
DK3419991T3 (da) | Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer | |
AR095348A1 (es) | Medios de cultivo celular y métodos de producción de anticuerpos | |
BR112016028512A8 (pt) | método de cultivo de uma célula de mamífero e método de produção de uma proteína recombinante | |
BR112017009152A2 (pt) | métodos de produção de proteínas de duas cadeias em bactérias | |
EP3162892A4 (en) | Method for culturing adhesive cells, culture vessel, and method for producing protein | |
BR112017020513A2 (pt) | método de produção de peixe para transplante, peixe para transplante, método de produção de espécie híbrida de peixe e espécie híbrida de peixe | |
PH12016502406A1 (en) | Cell culture process for producing a protein | |
EA202190244A3 (ru) | Сверхэкспрессия регуляторов пути n-гликозилирования для модуляции гликозилирования рекомбинантных белков | |
SG11201610878QA (en) | Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same | |
MY184085A (en) | Method of protein manufacture | |
EP3194573A4 (en) | Mammalian pluripotent stem cells, methods for their production, and uses thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 3A ANUIDADE. |
|
B08G | Application fees: restoration [chapter 8.7 patent gazette] | ||
B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
|
B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] |