BR112017008938A2 - protease inteína-modificada, cassete de expressão, composição, produto de cuidados domésticos, método de regular a atividade de protease e método de armazenar uma protease em uma mistura - Google Patents
protease inteína-modificada, cassete de expressão, composição, produto de cuidados domésticos, método de regular a atividade de protease e método de armazenar uma protease em uma misturaInfo
- Publication number
- BR112017008938A2 BR112017008938A2 BR112017008938A BR112017008938A BR112017008938A2 BR 112017008938 A2 BR112017008938 A2 BR 112017008938A2 BR 112017008938 A BR112017008938 A BR 112017008938A BR 112017008938 A BR112017008938 A BR 112017008938A BR 112017008938 A2 BR112017008938 A2 BR 112017008938A2
- Authority
- BR
- Brazil
- Prior art keywords
- protease
- storing
- whole
- mixture
- modified
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38663—Stabilised liquid enzyme compositions
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
Abstract
a presente invenção refere-se a métodos para a produção de uma protease inteína-modificada de trans-splicing com solubilidade aumentada e regulação de sua atividade. a presente invenção refere-se ainda a proteases inteína-modificadas de solubilidade aumentada e polinucleotídeos que codificam a mesma. a presente invenção refere-se também a métodos de armazenamento de proteases de trans-splicing.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462069653P | 2014-10-28 | 2014-10-28 | |
US62/069,653 | 2014-10-28 | ||
PCT/US2015/057862 WO2016069774A1 (en) | 2014-10-28 | 2015-10-28 | Methods and compositions for stabilizing trans-splicing intein modified proteases |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112017008938A2 true BR112017008938A2 (pt) | 2018-01-16 |
BR112017008938B1 BR112017008938B1 (pt) | 2023-09-26 |
Family
ID=55858316
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017008938-6A BR112017008938B1 (pt) | 2014-10-28 | 2015-10-28 | Composição, cassete de expressão, produto de cuidados domésticos, método de regular a atividade de protease e método de armazenar uma protease em uma mistura |
Country Status (7)
Country | Link |
---|---|
US (2) | US10731143B2 (pt) |
EP (1) | EP3212778B1 (pt) |
CN (1) | CN107075491B (pt) |
BR (1) | BR112017008938B1 (pt) |
CA (1) | CA2963148C (pt) |
DK (1) | DK3212778T3 (pt) |
WO (1) | WO2016069774A1 (pt) |
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US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
KR101891455B1 (ko) | 2014-11-03 | 2018-08-27 | 메르크 파텐트 게엠베하 | 생체분자를 정제하기 위한 가용성 인테인 융합 단백질 및 방법 |
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WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
CN110214180A (zh) | 2016-10-14 | 2019-09-06 | 哈佛大学的校长及成员们 | 核碱基编辑器的aav递送 |
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US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (en) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
CN110914426A (zh) | 2017-03-23 | 2020-03-24 | 哈佛大学的校长及成员们 | 包含核酸可编程dna结合蛋白的核碱基编辑器 |
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WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
JP2021500036A (ja) | 2017-10-16 | 2021-01-07 | ザ ブロード インスティテュート, インコーポレーテッドThe Broad Institute, Inc. | アデノシン塩基編集因子の使用 |
CN108004220B (zh) * | 2017-12-25 | 2021-01-29 | 刘丹妮 | 提高热稳定性的碱性蛋白酶BmP突变体及其基因和应用 |
EP3867387A2 (en) * | 2018-10-15 | 2021-08-25 | Fondazione Telethon | Intein proteins and uses thereof |
ES2955774T3 (es) | 2018-11-29 | 2023-12-07 | Henkel Ag & Co Kgaa | Variantes de proteasa de rendimiento mejorado y almacenamiento estable |
EP3660146B1 (de) * | 2018-11-29 | 2023-11-15 | Henkel AG & Co. KGaA | Leistungsverbesserte und lagerstabile protease-varianten |
BR112021018607A2 (pt) | 2019-03-19 | 2021-11-23 | Massachusetts Inst Technology | Métodos e composições para editar sequências de nucleotídeos |
US20220204975A1 (en) | 2019-04-12 | 2022-06-30 | President And Fellows Of Harvard College | System for genome editing |
GB201909491D0 (en) * | 2019-07-01 | 2019-08-14 | Governing Council Of The Univ Of Toronto | Detection of protein to protein interactions |
US20220315906A1 (en) | 2019-08-08 | 2022-10-06 | The Broad Institute, Inc. | Base editors with diversified targeting scope |
US20220332757A1 (en) * | 2019-09-09 | 2022-10-20 | Wuhan Yzy Biopharma Co., Ltd. | Split intein and preparation method for recombinant polypeptide using the same |
WO2021072328A1 (en) | 2019-10-10 | 2021-04-15 | The Broad Institute, Inc. | Methods and compositions for prime editing rna |
WO2021158999A1 (en) | 2020-02-05 | 2021-08-12 | The Broad Institute, Inc. | Gene editing methods for treating spinal muscular atrophy |
WO2021183693A1 (en) | 2020-03-11 | 2021-09-16 | The Broad Institute, Inc. | Stat3-targeted based editor therapeutics for the treatment of melanoma and other cancers |
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CN116096873A (zh) | 2020-05-08 | 2023-05-09 | 布罗德研究所股份有限公司 | 同时编辑靶标双链核苷酸序列的两条链的方法和组合物 |
AU2021350835A1 (en) | 2020-09-24 | 2023-04-27 | President And Fellows Of Harvard College | Prime editing guide rnas, compositions thereof, and methods of using the same |
WO2022150790A2 (en) | 2021-01-11 | 2022-07-14 | The Broad Institute, Inc. | Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision |
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-
2015
- 2015-10-28 CN CN201580058516.4A patent/CN107075491B/zh active Active
- 2015-10-28 BR BR112017008938-6A patent/BR112017008938B1/pt active IP Right Grant
- 2015-10-28 CA CA2963148A patent/CA2963148C/en active Active
- 2015-10-28 WO PCT/US2015/057862 patent/WO2016069774A1/en active Application Filing
- 2015-10-28 US US15/519,602 patent/US10731143B2/en active Active
- 2015-10-28 EP EP15854157.3A patent/EP3212778B1/en active Active
- 2015-10-28 DK DK15854157T patent/DK3212778T3/da active
-
2020
- 2020-06-11 US US16/899,064 patent/US11066657B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP3212778A1 (en) | 2017-09-06 |
US11066657B2 (en) | 2021-07-20 |
CA2963148C (en) | 2024-04-30 |
US20180298368A1 (en) | 2018-10-18 |
EP3212778B1 (en) | 2019-08-28 |
US20200308564A1 (en) | 2020-10-01 |
CN107075491B (zh) | 2021-07-06 |
WO2016069774A1 (en) | 2016-05-06 |
DK3212778T3 (da) | 2019-11-04 |
CA2963148A1 (en) | 2016-05-06 |
BR112017008938B1 (pt) | 2023-09-26 |
US10731143B2 (en) | 2020-08-04 |
EP3212778A4 (en) | 2018-05-02 |
CN107075491A (zh) | 2017-08-18 |
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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 28/10/2015, OBSERVADAS AS CONDICOES LEGAIS |