BR112015030208A2 - Métodos para produzir um produto de fermentação e um micro-organismo, e, micro-organismo recombinante - Google Patents

Métodos para produzir um produto de fermentação e um micro-organismo, e, micro-organismo recombinante

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Publication number
BR112015030208A2
BR112015030208A2 BR112015030208A BR112015030208A BR112015030208A2 BR 112015030208 A2 BR112015030208 A2 BR 112015030208A2 BR 112015030208 A BR112015030208 A BR 112015030208A BR 112015030208 A BR112015030208 A BR 112015030208A BR 112015030208 A2 BR112015030208 A2 BR 112015030208A2
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BR
Brazil
Prior art keywords
microorganism
fermentation
producing
recombinant
fermentation product
Prior art date
Application number
BR112015030208A
Other languages
English (en)
Inventor
Kopke Michael
Paul Mueller Alexander
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of BR112015030208A2 publication Critical patent/BR112015030208A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07001—Pyruvate synthase (1.2.7.1), i.e. pyruvate ferredoxin oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07005—Aldehyde ferredoxin oxidoreductase (1.2.7.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01196—Cobalt-precorrin-7 (C15)-methyltransferase (decarboxylating) (2.1.1.196)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MÉTODO PARA PRODUZIR UM PRODUTO DE FERMENTAÇÃO E UM MICRO-ORGANISMO E MICRO-ORGANISMO RECOMBINANTE. A invenção fornece métodos para aumentar a produção de produtos de fermentação através do aumento do fluxo através de uma via de fermentação pela otimização de reações enzimáticas. Em particular, a invenção se refere a identificação de enzimas e/ou fatores envolvidos em congestionamentos metabólicos em vias de fermentação e a fermentação de um substrato que compreende CO com um micro-organismo Clostridia carboxitrófico recombinante adaptada para exibir atividade aumentada das uma ou mais das ditas enzimas ou disponibilidade aumentada dos um ou mais dos ditos cofatores em comparação a um micro-organismo parental.
BR112015030208A 2013-06-05 2014-06-05 Métodos para produzir um produto de fermentação e um micro-organismo, e, micro-organismo recombinante BR112015030208A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361831591P 2013-06-05 2013-06-05
PCT/US2014/041188 WO2014197746A1 (en) 2013-06-05 2014-06-05 Recombinant microorganisms exhibiting increased flux through a fermentation pathway

Publications (1)

Publication Number Publication Date
BR112015030208A2 true BR112015030208A2 (pt) 2017-08-22

Family

ID=52008601

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015030208A BR112015030208A2 (pt) 2013-06-05 2014-06-05 Métodos para produzir um produto de fermentação e um micro-organismo, e, micro-organismo recombinante

Country Status (10)

Country Link
US (1) US20150079650A1 (pt)
EP (1) EP3004362A4 (pt)
JP (1) JP2016520325A (pt)
KR (1) KR20160018568A (pt)
CN (1) CN105283554A (pt)
AU (2) AU2014274838A1 (pt)
BR (1) BR112015030208A2 (pt)
CA (1) CA2914003C (pt)
EA (1) EA201592169A1 (pt)
WO (1) WO2014197746A1 (pt)

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CN104515851B (zh) * 2015-01-08 2016-09-14 河南农业大学 一种直接测定厌氧微生物内氢化酶或一氧化碳脱氢酶活性的方法与装置
CN105624182A (zh) * 2015-12-15 2016-06-01 重庆大学 生产四氢叶酸的重组质粒、基因工程菌株构建及应用
US10240170B2 (en) * 2016-01-06 2019-03-26 C1Chem Co., Ltd. CO hydratase and method for producing formate using the same
KR20230044031A (ko) * 2016-05-14 2023-03-31 란자테크, 인크. 알데히드:페레독신 옥시도리덕타제 활성이 변경된 미생물 및 관련 방법
CN108330094B (zh) * 2018-01-10 2020-04-21 上海晶诺生物科技有限公司 一种产烟酸的游离型重组耻垢分枝杆菌及其构建方法
CN109810991B (zh) * 2019-03-02 2021-11-12 昆明理工大学 二氢蝶酸合酶基因folP的用途
WO2020188033A1 (en) 2019-03-20 2020-09-24 Global Bioenergies Improved means and methods for producing isobutene from acetyl-coa
CN111089914A (zh) * 2019-11-07 2020-05-01 河北首朗新能源科技有限公司 微生物发酵生产乙醇中代谢物的高效液相检测方法
CN111235169A (zh) * 2020-02-03 2020-06-05 昆明理工大学 一种GTP环化水解酶I基因folE及应用
CN114480562B (zh) * 2022-01-27 2023-09-19 西南医科大学附属医院 一种基于pfor酶活性导向的fmt供体筛选方法及其应用

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Also Published As

Publication number Publication date
US20150079650A1 (en) 2015-03-19
AU2018204112A1 (en) 2018-06-28
JP2016520325A (ja) 2016-07-14
WO2014197746A1 (en) 2014-12-11
KR20160018568A (ko) 2016-02-17
CA2914003C (en) 2018-01-02
EP3004362A1 (en) 2016-04-13
EA201592169A1 (ru) 2016-04-29
CA2914003A1 (en) 2014-12-11
EP3004362A4 (en) 2017-01-11
CN105283554A (zh) 2016-01-27
AU2014274838A1 (en) 2015-12-24

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