BR112015001054A8 - Helicase monomérica que é membro da superfamília 1 ou superfamília 2, helicase hel308 isolada, método para caracterizar um polinucleotídeo alvo, e, sensor para a caracterização de um polinucleotídeo alvo - Google Patents

Helicase monomérica que é membro da superfamília 1 ou superfamília 2, helicase hel308 isolada, método para caracterizar um polinucleotídeo alvo, e, sensor para a caracterização de um polinucleotídeo alvo

Info

Publication number
BR112015001054A8
BR112015001054A8 BR112015001054A BR112015001054A BR112015001054A8 BR 112015001054 A8 BR112015001054 A8 BR 112015001054A8 BR 112015001054 A BR112015001054 A BR 112015001054A BR 112015001054 A BR112015001054 A BR 112015001054A BR 112015001054 A8 BR112015001054 A8 BR 112015001054A8
Authority
BR
Brazil
Prior art keywords
target polynucleotide
superfamily
helicase
characterizing
sensor
Prior art date
Application number
BR112015001054A
Other languages
English (en)
Other versions
BR112015001054A2 (pt
Inventor
Heron Andrew
Clarke James
Moysey Ruth
Jayne Wallace Elizabeth
Bruce Mark
Jayasinghe Lakmal
Caprotti Domenico
Soeroes Szabolcs
Mcneill Luke
Alves David
Bowen Rebecca
Milton John
Original Assignee
Oxford Nanopore Tech Ltd
Oxford Nanopore Tech Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Tech Ltd, Oxford Nanopore Tech Plc filed Critical Oxford Nanopore Tech Ltd
Publication of BR112015001054A2 publication Critical patent/BR112015001054A2/pt
Publication of BR112015001054A8 publication Critical patent/BR112015001054A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04012—DNA helicase (3.6.4.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)
    • C12Y599/01002—DNA topoisomerase (5.99.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)
    • C12Y599/01003—DNA topoisomerase (ATP-hydrolysing) (5.99.1.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Abstract

HELICASE FORMADA A PARTIR DE UM OU MAIS MONÔMEROS, CONSTRUTO, MÉTODOS PARA CARACTERIZAR UM POLINUCLEOTÍDEO ALVO, SENSOR PARA A CARACTERIZAÇÃO DE UM POLINUCLEOTÍDEO ALVO, E, USO DE UMA HELICASE. A invenção refere-se a helicases modificadas com desaglutinação modificado de polinucleotídeos. As helicases podem ser usadas para controlar o movimento dos polinucleotídeos e são particularmente úteis para sequenciamento de polinucleotídeos.
BR112015001054A 2012-07-19 2013-07-18 Helicase monomérica que é membro da superfamília 1 ou superfamília 2, helicase hel308 isolada, método para caracterizar um polinucleotídeo alvo, e, sensor para a caracterização de um polinucleotídeo alvo BR112015001054A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261673452P 2012-07-19 2012-07-19
US201361774862P 2013-03-08 2013-03-08
PCT/GB2013/051925 WO2014013260A1 (en) 2012-07-19 2013-07-18 Modified helicases

Publications (2)

Publication Number Publication Date
BR112015001054A2 BR112015001054A2 (pt) 2017-07-25
BR112015001054A8 true BR112015001054A8 (pt) 2022-08-02

Family

ID=48874432

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015001054A BR112015001054A8 (pt) 2012-07-19 2013-07-18 Helicase monomérica que é membro da superfamília 1 ou superfamília 2, helicase hel308 isolada, método para caracterizar um polinucleotídeo alvo, e, sensor para a caracterização de um polinucleotídeo alvo

Country Status (7)

Country Link
US (3) US10808231B2 (pt)
EP (2) EP2875128B8 (pt)
JP (2) JP6614972B2 (pt)
AU (1) AU2013291763B2 (pt)
BR (1) BR112015001054A8 (pt)
CA (1) CA2879261C (pt)
WO (1) WO2014013260A1 (pt)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CN102369298B (zh) 2009-01-30 2017-03-22 牛津纳米孔技术有限公司 跨膜测序中用于核酸构建体的衔接体
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
IN2014DN00221A (pt) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
CA2866587C (en) 2012-02-16 2022-07-26 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
EP2836506B1 (en) 2012-04-10 2017-04-19 Oxford Nanopore Technologies Limited Mutant lysenin pores
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
EP2964779B1 (en) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN105899678A (zh) 2013-10-18 2016-08-24 牛津纳米孔技术公司 经修饰的酶
SI3074534T1 (sl) * 2013-11-26 2019-08-30 Illumina, Inc. Postopki določanja zaporedja polinukleotidov
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3108229A4 (en) 2014-02-19 2017-09-06 University of Washington Nanopore-based analysis of protein characteristics
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CN106459159B (zh) 2014-05-02 2021-11-30 牛津纳米孔技术公司 突变孔
CN117164684A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
KR102551897B1 (ko) 2014-10-16 2023-07-06 옥스포드 나노포어 테크놀로지즈 피엘씨 폴리머의 분석
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
US10480026B2 (en) 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
CA2967036A1 (en) * 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
EP3254103B1 (en) 2015-02-05 2023-08-16 President and Fellows of Harvard College Nanopore sensor including fluidic passage
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
CN108027335B (zh) 2015-06-25 2021-05-04 罗斯韦尔生物技术股份有限公司 生物分子传感器和方法
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
WO2017132567A1 (en) 2016-01-28 2017-08-03 Roswell Biotechnologies, Inc. Massively parallel dna sequencing apparatus
JP7280590B2 (ja) 2016-01-28 2023-05-24 ロズウェル バイオテクノロジーズ，インコーポレイテッド 大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置
JP6854532B2 (ja) 2016-02-09 2021-04-07 ロズウェル バイオテクノロジーズ，インコーポレイテッド 電子的、標識フリーのｄｎａおよびゲノムシークエンシング
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
CA3212147A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Plc Mutant pore
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017203268A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
EP3491150A4 (en) * 2016-08-01 2020-03-11 Roswell Biotechnologies, Inc MODIFIED NUCLEOTIDE TRIPHOSPHATES FOR MOLECULAR ELECTRONIC SENSORS
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
EP3568407A4 (en) 2017-01-10 2020-12-23 Roswell Biotechnologies, Inc DNA DATA STORAGE METHODS AND SYSTEMS
WO2018136148A1 (en) 2017-01-19 2018-07-26 Roswell Biotechnologies, Inc. Solid state sequencing devices comprising two dimensional layer materials
US11085071B2 (en) * 2017-02-01 2021-08-10 Spot Biosystems Ltd. Highly stable and specific molecular beacons encapsulated in cationic lipoplex nanoparticles and application thereof
KR20200002897A (ko) 2017-04-25 2020-01-08 로스웰 바이오테크놀로지스 인코포레이티드 분자 센서들을 위한 효소 회로들
US10508296B2 (en) 2017-04-25 2019-12-17 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
EP4023764A3 (en) 2017-05-09 2022-09-21 Roswell Biotechnologies, Inc. Binding probe circuits for molecular sensors
KR20200030070A (ko) 2017-06-30 2020-03-19 브이아이비 브이지더블유 신규한 단백질 포어
WO2019046589A1 (en) 2017-08-30 2019-03-07 Roswell Biotechnologies, Inc. PROCESSIVE ENZYME MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA
CN111373051A (zh) 2017-10-10 2020-07-03 罗斯威尔生命技术公司 用于无扩增dna数据存储的方法、装置和***
GB2569977A (en) 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201808556D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201808554D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
US20220162692A1 (en) 2018-07-30 2022-05-26 Oxford University Innovation Limited Assemblies
JP2020031557A (ja) * 2018-08-28 2020-03-05 株式会社日立ハイテクノロジーズ 生体分子分析装置
CN112805393A (zh) * 2018-09-28 2021-05-14 北京齐碳科技有限公司 一种解旋酶及其应用
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
EP4270008A3 (en) 2019-12-02 2024-01-10 Oxford Nanopore Technologies PLC Method of characterising a target polypeptide using a nanopore
GB201917742D0 (en) 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
NL2024579B1 (en) 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
WO2021255476A2 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited Method
WO2021255475A1 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited A method of selectively characterising a polynucleotide using a detector
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
KR20220124741A (ko) * 2020-06-19 2022-09-14 치탄 테크놀로지 리미티드 베이징 Pif1 유사 헬리카제 및 이의 용도
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
US11248265B1 (en) 2020-11-19 2022-02-15 Clear Labs, Inc Systems and processes for distinguishing pathogenic and non-pathogenic sequences from specimens
WO2022126304A1 (zh) * 2020-12-14 2022-06-23 北京齐碳科技有限公司 一种经修饰的解旋酶及其应用
GB202103605D0 (en) 2021-03-16 2021-04-28 Oxford Nanopore Tech Ltd Alignment of target and reference sequences of polymer units
CN115777019A (zh) 2021-04-06 2023-03-10 成都齐碳科技有限公司 经修饰的Prp43解旋酶及其用途
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
EP4341433A1 (en) 2021-05-19 2024-03-27 Oxford Nanopore Technologies PLC Methods for complement strand sequencing
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
GB202112235D0 (en) 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore
WO2023057935A1 (en) * 2021-10-06 2023-04-13 Seqirus Inc. Lipid nanoparticle comprising a nucleic acid-binding protein
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
WO2023163946A1 (en) * 2022-02-22 2023-08-31 Peter Biotherapeutics, Inc. Technologies for genetic modification
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
US20240026427A1 (en) 2022-05-06 2024-01-25 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024033443A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
CN117721101A (zh) * 2022-09-16 2024-03-19 北京普译生物科技有限公司 一种经修饰的CaPif1解旋酶及其应用
WO2024089270A2 (en) 2022-10-28 2024-05-02 Oxford Nanopore Technologies Plc Pore monomers and pores
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602979B1 (en) 1994-12-19 2003-08-05 The United States Of America As Represented By The Department Of Health And Human Services Screening assays for compounds that cause apoptosis
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2002092821A1 (fr) 2001-05-01 2002-11-21 National Institute Of Advanced Industrial Science And Technology Nouveau maxizyme
WO2002103210A1 (en) 2001-06-15 2002-12-27 Hansford Derek J Nanopump devices and methods
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
WO2004092331A2 (en) 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
US7851203B2 (en) 2003-10-01 2010-12-14 Lawrence Livermore National Security, Llc Functionalized apertures for the detection of chemical and biological materials
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US8008461B2 (en) 2004-10-27 2011-08-30 Kaneka Corporation Carbonyl reductase, gene therefor and use thereof
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
JP2009513125A (ja) 2005-10-28 2009-04-02 ビオメリュー・ソシエテ・アノニム がん検出方法
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP2195648B1 (en) 2007-09-12 2019-05-08 President and Fellows of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
CN102144037A (zh) 2008-07-07 2011-08-03 牛津纳米孔技术有限公司 检测碱基的孔
WO2010004265A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102369298B (zh) 2009-01-30 2017-03-22 牛津纳米孔技术有限公司 跨膜测序中用于核酸构建体的衔接体
WO2010086603A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Enzyme mutant
EP2398494A4 (en) 2009-02-23 2015-10-28 Cytomx Therapeutics Inc Proproteins and their methods of use
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
DK2422198T3 (da) 2009-04-20 2014-01-06 Oxford Nanopore Tech Ltd Lipiddobbeltlag-sensorgruppe
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
JP5873023B2 (ja) 2009-12-01 2016-03-01 オックスフォード ナノポール テクノロジーズ リミテッド 生化学分析機器
WO2011151941A1 (ja) 2010-06-04 2011-12-08 国立大学法人東京大学 制御性ｔ細胞の増殖または集積を誘導する作用を有する組成物
CN103392008B (zh) 2010-09-07 2017-10-20 加利福尼亚大学董事会 通过持续性酶以一个核苷酸的精度控制dna在纳米孔中的移动
WO2012098561A2 (en) 2011-01-18 2012-07-26 Swamy R K A multipupurpose instrument for triangle solutions, measurements and geometrical applications called triometer
WO2012098562A2 (en) 2011-01-19 2012-07-26 Panacea Biotec Limited Liquid oral compositions of lanthanum salts
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
IN2014DN00221A (pt) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
US20160162634A1 (en) 2011-09-23 2016-06-09 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
EP2836506B1 (en) 2012-04-10 2017-04-19 Oxford Nanopore Technologies Limited Mutant lysenin pores
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
CN104936682B (zh) 2012-10-26 2017-12-15 牛津纳米孔技术公司 微滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
EP2964779B1 (en) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
WO2014158665A1 (en) 2013-03-14 2014-10-02 Arkema Inc. Methods for crosslinking polymer compositions in the presence of atmospheric oxygen
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN105899678A (zh) 2013-10-18 2016-08-24 牛津纳米孔技术公司 经修饰的酶
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CN106459159B (zh) 2014-05-02 2021-11-30 牛津纳米孔技术公司 突变孔
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items
CN117164684A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
US10480026B2 (en) 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
WO2017203268A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method

Also Published As

Publication number Publication date
CA2879261A1 (en) 2014-01-23
EP3741847A1 (en) 2020-11-25
US11525126B2 (en) 2022-12-13
US20230227799A1 (en) 2023-07-20
EP2875128B1 (en) 2020-05-20
US20150191709A1 (en) 2015-07-09
WO2014013260A1 (en) 2014-01-23
JP2015528700A (ja) 2015-10-01
JP2019213529A (ja) 2019-12-19
US10808231B2 (en) 2020-10-20
AU2013291763A1 (en) 2015-02-05
US20210123032A1 (en) 2021-04-29
AU2013291763B2 (en) 2019-06-20
EP2875128A1 (en) 2015-05-27
BR112015001054A2 (pt) 2017-07-25
EP2875128B8 (en) 2020-06-24
CA2879261C (en) 2022-12-06
JP6614972B2 (ja) 2019-12-04

Similar Documents

Publication Publication Date Title
BR112015001054A8 (pt) Helicase monomérica que é membro da superfamília 1 ou superfamília 2, helicase hel308 isolada, método para caracterizar um polinucleotídeo alvo, e, sensor para a caracterização de um polinucleotídeo alvo
BR112015001052B8 (pt) Métodos para distinguir um polinucleotídeo alvo,sensor para distinguir um polinucleotídeo alvo, e, uso de um construto
WO2015055981A3 (en) Modified helicases
WO2016055777A3 (en) Modified enzymes
CA2865388C (en) Novel fgfr3 fusion
BR112015001690A2 (pt) mutações associadas com a resistência a inibidores da tirosina quinase de bruton (btk)
BR112017006736A2 (pt) anticorpos antimiostatina, polipeptídeos contendo regiões fc variantes, e métodos de utilização
BR112018010805A2 (pt) mutantes de proteína f de rsv
CL2016002235A1 (es) Composiciones útiles en el tratamiento de deficiencia de ornitina-transcarbamilasa (otc)
BR112017023872A2 (pt) anticorpos anti-cd166, anticorpos anti-cd166 ativáveis e métodos de uso dos mesmos
EP3636170C0 (en) TIME-DEPENDENT EVALUATION OF SENSOR DATA TO DETERMINE THE STABILITY, CREEP AND VISCOELASTIC ELEMENTS OF MEASUREMENTS
BR112016024494A2 (pt) análogos de peptídeo com sonda(s) de aminoácidos ramificados
EA201791735A1 (ru) Связывающие молекулы, направленные против гемагглютинина вируса гриппа, и пути их применения
BR112015001381A2 (pt) faixa dinâmica ampla com o uso de sensor monocromático
BR112015022763A8 (pt) sensor para detectar um analito
BR112015028449A2 (pt) atribuir ação do usuário com base em identidade biométrica
BR112014008597A2 (pt) sistema e método para determinação dos pontos q e j de um eletrocardiograma (ecg); um ou mais processadores; e mídia executável por computador não transitória
BR112017019378A2 (pt) análogos de amilina
BR112016029465A2 (pt) chapa de aço elétrica
EA201691263A1 (ru) Полимер и асфальтовая композиция
BR112014019571A8 (pt) Composição de limpeza espumante
BR112016027024A2 (pt) polipeptídeos de ligação específica e seus usos
EP3268732A4 (en) Nanopore detection of small molecules through competition assays
EP4321867A3 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
BR112012027273A2 (pt) aparelho de determinação de propriedade para a determinação de uma propriedade de um objeto biológico, aparelho de influência ao objeto para influenciar um objeto biológico, programa de computador para a determinação de propriedade para determinar uma propriedade de um objeto biológico e programa de computador de influência ao objeto para influenciar um objeto biológico

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B25D Requested change of name of applicant approved

Owner name: OXFORD NANOPORE TECHNOLOGIES PLC (GB)

B25G Requested change of headquarter approved

Owner name: OXFORD NANOPORE TECHNOLOGIES PLC (GB)