BR112015000534A8 - “método para a preparação de 2,4-dihidroxibutirato, microrganismo modificado e método de produção de 2,4-dhb” - Google Patents

“método para a preparação de 2,4-dihidroxibutirato, microrganismo modificado e método de produção de 2,4-dhb”

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Publication number
BR112015000534A8
BR112015000534A8 BR112015000534A BR112015000534A BR112015000534A8 BR 112015000534 A8 BR112015000534 A8 BR 112015000534A8 BR 112015000534 A BR112015000534 A BR 112015000534A BR 112015000534 A BR112015000534 A BR 112015000534A BR 112015000534 A8 BR112015000534 A8 BR 112015000534A8
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Brazil
Prior art keywords
dihydroxybutyrate
preparation
dhb
production
modified microorganism
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BR112015000534A
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English (en)
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BR112015000534B1 (pt
BR112015000534A2 (pt
Inventor
Walther Thomas
Dressaire Clémentine
Cordier Hélène
Marie Francois Jean
Huet Robert
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Adisseo France Sas
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Publication of BR112015000534A2 publication Critical patent/BR112015000534A2/pt
Publication of BR112015000534A8 publication Critical patent/BR112015000534A8/pt
Publication of BR112015000534B1 publication Critical patent/BR112015000534B1/pt

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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01082—Malate dehydrogenase (NADP+) (1.1.1.82)
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01299—Malate dehydrogenase (NAD(P)+)(1.1.1.299)
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    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01001—Aspartate transaminase (2.6.1.1), i.e. aspartate-aminotransferase
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    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01042—Branched-chain-amino-acid transaminase (2.6.1.42)

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

MÉTODO PARA A PREPARAÇÃO DE 2,4-DIHIDROXIBUTIRATO, MICRORGANISMO MODIFICADO, MÉTODO DE PRODUÇÃO DE 2,4-DHB E USO A presente invenção refere-se a um método para a preparação de ácido 2,4-dihidroxibutírico a partir de homoserina, que compreende uma primeira etapa de conversão do grupo amino primário de homoserina para um grupo carbonila para obter 2-oxo-4-hidroxibutirato, e uma segunda etapa de redução do 2-oxo-4-hidroxibutirato (OHB) para 2,4-dihidroxibutirato.
BR112015000534-9A 2012-07-11 2013-07-10 Método para a preparação de 2,4-dihidroxibutirato, microrganismo modificado e método de produção de 2,4-dhb BR112015000534B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261670405P 2012-07-11 2012-07-11
US61/670,405 2012-07-11
PCT/EP2013/064619 WO2014009435A1 (en) 2012-07-11 2013-07-10 Method for the preparation of 2,4-dihydroxybutyrate

Publications (3)

Publication Number Publication Date
BR112015000534A2 BR112015000534A2 (pt) 2017-12-19
BR112015000534A8 true BR112015000534A8 (pt) 2023-01-03
BR112015000534B1 BR112015000534B1 (pt) 2023-02-28

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BR112015000534-9A BR112015000534B1 (pt) 2012-07-11 2013-07-10 Método para a preparação de 2,4-dihidroxibutirato, microrganismo modificado e método de produção de 2,4-dhb

Country Status (11)

Country Link
US (2) US10570422B2 (pt)
EP (1) EP2872640B1 (pt)
JP (1) JP6293746B2 (pt)
KR (1) KR102093172B1 (pt)
CN (1) CN104471069B (pt)
AR (1) AR091726A1 (pt)
BR (1) BR112015000534B1 (pt)
ES (1) ES2800341T3 (pt)
IN (1) IN2014DN10637A (pt)
RU (1) RU2645260C2 (pt)
WO (1) WO2014009435A1 (pt)

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US10385322B2 (en) 2014-10-03 2019-08-20 Metabolic Explorer Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
US10415062B2 (en) 2015-04-07 2019-09-17 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxybutyrate
WO2016162442A1 (en) 2015-04-07 2016-10-13 Metabolic Explorer A modified microorganism for the optimized production of 2,4-dihydroxyburyrate with enhanced 2,4-dihydroxybutyrate efflux
JP7071124B2 (ja) 2015-06-04 2022-05-18 ビーエーエスエフ ソシエタス・ヨーロピア ファインケミカルの生産改善のための組換え微生物
JP2018516587A (ja) * 2015-06-12 2018-06-28 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ アラニンの生産改善のための組換え微生物
BR112017027815B1 (pt) 2015-06-25 2021-12-21 Dynamic Food Ingredients Corporation Método de produção de 2,4-dihidroxibutirato
WO2019013573A2 (ko) * 2017-07-12 2019-01-17 울산과학기술원 2-히드록시 감마 부티로락톤 또는 2, 4-디히드록시 -부티레이트 의 제조 방법
EP3470512A1 (en) 2017-10-10 2019-04-17 Metabolic Explorer Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
CN108866017B (zh) * 2018-08-24 2020-07-03 浙江华睿生物技术有限公司 一种酶法制备β-羟基-β-甲基丁酸的方法
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CN110577961B (zh) * 2019-09-23 2021-05-04 安徽师范大学 一种热稳定性苹果酸脱氢酶基因的构建方法、编码蛋白及其应用
DE102021101004B3 (de) 2021-01-19 2022-03-10 Technische Universität Dresden, Körperschaft des öffentlichen Rechts Verfahren zur Herstellung von 2,4-Dihydroxybutyrat oder L-Threonin unter Nutzung eines mikrobiellen Stoffwechselweges
CN113106029A (zh) * 2021-04-20 2021-07-13 南京工业大学 一种过表达aro8基因的酿酒酵母工程菌及其构建方法与应用
WO2023230399A1 (en) * 2022-05-23 2023-11-30 The Regents Of The University Of California Novel carbon fixation pathway
CN115948482B (zh) * 2023-02-07 2024-02-09 中国科学院天津工业生物技术研究所 一种2，4-二羟基丁酸生物合成途径的构建方法及应用

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US20200131542A1 (en) 2020-04-30
JP2015524253A (ja) 2015-08-24
CN104471069B (zh) 2018-06-01
KR20150035726A (ko) 2015-04-07
AR091726A1 (es) 2015-02-25
WO2014009435A1 (en) 2014-01-16
RU2014153354A (ru) 2016-08-27
BR112015000534B1 (pt) 2023-02-28
JP6293746B2 (ja) 2018-03-14
RU2645260C2 (ru) 2018-02-19
US10570422B2 (en) 2020-02-25
ES2800341T3 (es) 2020-12-29
BR112015000534A2 (pt) 2017-12-19
EP2872640B1 (en) 2020-03-25
KR102093172B1 (ko) 2020-03-25
CN104471069A (zh) 2015-03-25
IN2014DN10637A (pt) 2015-09-11
EP2872640A1 (en) 2015-05-20
US11505811B2 (en) 2022-11-22
US20150147793A1 (en) 2015-05-28

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