BR112014027378A2 - expressão transiente de alto rendimento em células de mamíferos usando pareamento exclusivo de crescimento de alta densidade e meio de transfecção e potenciadores de expressão - Google Patents

expressão transiente de alto rendimento em células de mamíferos usando pareamento exclusivo de crescimento de alta densidade e meio de transfecção e potenciadores de expressão

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BR112014027378A2
BR112014027378A2 BR112014027378A BR112014027378A BR112014027378A2 BR 112014027378 A2 BR112014027378 A2 BR 112014027378A2 BR 112014027378 A BR112014027378 A BR 112014027378A BR 112014027378 A BR112014027378 A BR 112014027378A BR 112014027378 A2 BR112014027378 A2 BR 112014027378A2
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cells
expression
medium
nucleic acid
acid molecules
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BR112014027378A
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Yan Liu Chao
C Chiou Henry
Rogers Jeffrey
Li Jingqiu
Cisneros Maria
Jones Meredith
K Vasu Sanjay
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Life Technologies Corp
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Abstract

resumo “expressão transiente de alto rendimento em células de mamíferos usando pareamento exclusivo de crescimento de alta densidade e meio de transfecção e potenciadores de expressão” a presente invenção é dirigida geralmente a meios de cultura celular (particularmente meios livres de soro, não derivados de animal, e/ou meios quimicamente definidos) que são úteis para a introdução de macromoléculas e compostos (por exemplo, moléculas de ácido nucleico) em células (por exemplo, células eucarióticas). de acordo com a invenção, esta introdução pode ocorrer na presença do referido meio. as células que contêm tais materiais introduzidos podem então ser cultivadas no meio e o efeito dos materiais introduzidos nas células pode ser medido ou determinado. em particular, a invenção permite a introdução de moléculas de ácido nucleico (por exemplo, vetores) nas células (particularmente células eucarióticas) e a expressão das proteínas codificadas pelas moléculas de ácido nucleico nas células. a presente invenção obvia a necessidade de mudar o meio de cultura celular cada vez que um procedimento diferente for realizado com as células (por exemplo, a cultura celular versus transfecção de células). a invenção também se refere a composições e kits úteis para a cultura e transformação/transfecção de células.
BR112014027378A 2012-05-02 2013-05-02 expressão transiente de alto rendimento em células de mamíferos usando pareamento exclusivo de crescimento de alta densidade e meio de transfecção e potenciadores de expressão BR112014027378A2 (pt)

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US201261641864P 2012-05-02 2012-05-02
PCT/US2013/039351 WO2013166339A1 (en) 2012-05-02 2013-05-02 High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers

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BR112014027378A2 true BR112014027378A2 (pt) 2017-06-27

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US (3) US10066000B2 (pt)
EP (3) EP4148122A1 (pt)
JP (2) JP6473079B2 (pt)
KR (2) KR102136698B1 (pt)
CN (2) CN109370980B (pt)
BR (1) BR112014027378A2 (pt)
CA (2) CA2872476C (pt)
WO (1) WO2013166339A1 (pt)

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JP6772309B2 (ja) 2020-10-21
EP2844737B1 (en) 2018-03-21
US20230130038A1 (en) 2023-04-27
JP2015515859A (ja) 2015-06-04
EP3404095A1 (en) 2018-11-21
US20190077841A1 (en) 2019-03-14
CN104364369B (zh) 2018-09-07
KR102292949B1 (ko) 2021-08-25
CN109370980B (zh) 2023-05-09
KR20200090935A (ko) 2020-07-29
CA2872476C (en) 2020-07-07
CA2872476A1 (en) 2013-11-07
KR20150063018A (ko) 2015-06-08
KR102136698B1 (ko) 2020-07-22
US11498949B2 (en) 2022-11-15
JP2019088304A (ja) 2019-06-13
WO2013166339A1 (en) 2013-11-07
EP2844737A1 (en) 2015-03-11
CA3051089C (en) 2022-04-12
US10066000B2 (en) 2018-09-04
CN109370980A (zh) 2019-02-22
CN104364369A (zh) 2015-02-18
EP4148122A1 (en) 2023-03-15
US20130316400A1 (en) 2013-11-28
JP6473079B2 (ja) 2019-02-20
EP3404095B1 (en) 2022-07-27
CA3051089A1 (en) 2013-11-07

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