GB901673A - Cell culture - Google Patents

Cell culture

Info

Publication number
GB901673A
GB901673A GB16237/58A GB1623758A GB901673A GB 901673 A GB901673 A GB 901673A GB 16237/58 A GB16237/58 A GB 16237/58A GB 1623758 A GB1623758 A GB 1623758A GB 901673 A GB901673 A GB 901673A
Authority
GB
United Kingdom
Prior art keywords
medium
cell
vessel
oxygen
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB16237/58A
Inventor
Peter Dodd Cooper
John Nivison Wilson
Avril Margaret Burt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK filed Critical National Research Development Corp UK
Priority to GB16237/58A priority Critical patent/GB901673A/en
Publication of GB901673A publication Critical patent/GB901673A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/87—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis the receptacle being divided into superimposed compartments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32611—Poliovirus
    • C12N2770/32651—Methods of production or purification of viral material

Abstract

901,673. Cultivating animal cells. NATIONAL RESEARCH DEVELOPMENT CORPORATION. May 19, 1959 [May 20, 1958], No. 16237/58. Class 81 (1). Animal cells, e.g. transformed or malignant cells from mammalean tissue such as lung, kidney, spleen, liver and testes, are cultured by suspending them in a liquid culture medium containing dissolved oxygen at a concentration varied to control the cell growth rate, cellcontaining medium being withdrawn and fresh medium being supplied at intervals to maintain the volume of medium substantially constant. The medium is agitated under an atmosphere containing from 5 to 25%, by volume, of oxygen and 0 to 10% carbon dioxide, the latter maintaining the pH at between 6.8 and 7.2, the preferred level, and being varied according to colorimetric readings on the medium which may contain phenol red. The process is preferably effected at 37 ‹ C. at a cell concentration of from 2 to 20 x 10<SP>5</SP> cells per millilitre of medium, a turbidimeter reactive to the cell concentration being used to control the oxygen concentration. A suitable culture medium comprises 0.5 to 2% by weight of glucose and/or fructose and/or galactose together with nitrogen sources, e.g. yeast extract, lactalbumin hydrolysate, L and DL methionine, L arginine and glutamic acid, with ammonium chloride and L-glutamine, vitamins, growth promoters, antibiotics, the medium being made isotonic with 0.9% aqueous sodium chloride solution. In the apparatus, Fig. 1, a stirred culture vessel 1 is supplied through a solenoid-operated valve 17a with culture medium from a vessel 16, cell-containing medium siphoning via a tube 2 to a cooled stirred storage vessel 5, all three vessels being supplied with manually-controlled amounts of oxygen, nitrogen and carbon dioxide from cylinders 15, 15<SP>1</SP>, 15<SP>11</SP>. Valve 17a is responsive to a time switch 17 controlled by a control box 18 connected to a recording device 18a and also to a turbidimeter comprising a light source 18b and a photo-cell 18c. An apparatus is also described, Figs. 2 and 3 (not shown), comprising several culture vessels, one above the other, in a single housing having a central common overflow tube serving each vessel.
GB16237/58A 1958-05-20 1958-05-20 Cell culture Expired GB901673A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB16237/58A GB901673A (en) 1958-05-20 1958-05-20 Cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB16237/58A GB901673A (en) 1958-05-20 1958-05-20 Cell culture

Publications (1)

Publication Number Publication Date
GB901673A true GB901673A (en) 1962-07-25

Family

ID=10073674

Family Applications (1)

Application Number Title Priority Date Filing Date
GB16237/58A Expired GB901673A (en) 1958-05-20 1958-05-20 Cell culture

Country Status (1)

Country Link
GB (1) GB901673A (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335215A (en) * 1980-08-27 1982-06-15 Monsanto Company Method of growing anchorage-dependent cells
EP0147975A2 (en) * 1983-12-28 1985-07-10 Ajinomoto Co., Inc. Method and apparatus for culture of animal cells
FR2578266A1 (en) * 1985-03-01 1986-09-05 New Brunswick Scient Cy Inc METHOD AND DEVICE FOR CONTROLLING A FERMENTER.
EP0348827A1 (en) * 1988-06-27 1990-01-03 Snow Brand Milk Products Co., Ltd. Method and apparatus for cell culture
US6103529A (en) * 1996-10-10 2000-08-15 Life Technologies, Inc. Animal cell culture media comprising peptides derived from rice
EP1382673A1 (en) * 2001-04-23 2004-01-21 Nitta Gelatin Inc. Method of culturing collected biopsy cells
EP2218775A1 (en) 1996-08-30 2010-08-18 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
EP2351827A1 (en) 2001-03-27 2011-08-03 Life Technologies Corporation Culture medium for cell growth and transfection
WO2013166339A1 (en) 2012-05-02 2013-11-07 Life Technologies Corporation High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers
EP3299454A1 (en) 2016-09-21 2018-03-28 Deutsches Krebsforschungszentrum Optimized method for large scale production of parvovirus h-1 in an essentially serum-free medium
CN109513415A (en) * 2018-12-26 2019-03-26 农业部规划设计研究院 A kind of acidification of rich water, storage processing device and method
CN112481266A (en) * 2020-12-17 2021-03-12 中国科学院深圳先进技术研究院 Gene element and using method thereof

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335215A (en) * 1980-08-27 1982-06-15 Monsanto Company Method of growing anchorage-dependent cells
EP0147975A3 (en) * 1983-12-28 1987-05-20 Ajinomoto Co., Inc. Method and apparatus for culture of animal cells
EP0147975A2 (en) * 1983-12-28 1985-07-10 Ajinomoto Co., Inc. Method and apparatus for culture of animal cells
FR2578266A1 (en) * 1985-03-01 1986-09-05 New Brunswick Scient Cy Inc METHOD AND DEVICE FOR CONTROLLING A FERMENTER.
EP0348827A1 (en) * 1988-06-27 1990-01-03 Snow Brand Milk Products Co., Ltd. Method and apparatus for cell culture
EP2221361A2 (en) 1996-08-30 2010-08-25 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
US9321996B2 (en) 1996-08-30 2016-04-26 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
US8815573B2 (en) 1996-08-30 2014-08-26 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
EP2218775A1 (en) 1996-08-30 2010-08-18 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
US8455246B2 (en) 1996-08-30 2013-06-04 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
EP2243827A1 (en) 1996-08-30 2010-10-27 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
US8785194B2 (en) 1996-08-30 2014-07-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
US8198084B2 (en) 1996-08-30 2012-06-12 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
US6103529A (en) * 1996-10-10 2000-08-15 Life Technologies, Inc. Animal cell culture media comprising peptides derived from rice
EP1983044A2 (en) 1996-10-10 2008-10-22 Invitrogen Corporation Animal cell culture media comprising plant-derived nutrients
US9879243B2 (en) 2001-03-27 2018-01-30 Lifetechnologies Corporation Culture medium for cell growth and transfection
EP2351827A1 (en) 2001-03-27 2011-08-03 Life Technologies Corporation Culture medium for cell growth and transfection
US8765464B2 (en) 2001-04-23 2014-07-01 Kurashiki Boseki Kabushiki Kaisha Kit for culturing cells comprising tube with flat wall, extracellular matrix and culture medium
US7247481B2 (en) 2001-04-23 2007-07-24 Nitta Gelatin Inc. Process for culturing cells sampled for biopsy
EP1382673A4 (en) * 2001-04-23 2004-06-09 Nitta Gelatin Kk Method of culturing collected biopsy cells
EP1382673A1 (en) * 2001-04-23 2004-01-21 Nitta Gelatin Inc. Method of culturing collected biopsy cells
WO2013166339A1 (en) 2012-05-02 2013-11-07 Life Technologies Corporation High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers
EP3404095A1 (en) 2012-05-02 2018-11-21 Life Technologies Corporation High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers
EP4148122A1 (en) 2012-05-02 2023-03-15 Life Technologies Corporation High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers
EP3299454A1 (en) 2016-09-21 2018-03-28 Deutsches Krebsforschungszentrum Optimized method for large scale production of parvovirus h-1 in an essentially serum-free medium
WO2018054720A1 (en) 2016-09-21 2018-03-29 Deutsches Krebsforschungszentrum Optimized method for !arge scale production of parvovirus h-1 in an essentially serum-free medium
CN109513415A (en) * 2018-12-26 2019-03-26 农业部规划设计研究院 A kind of acidification of rich water, storage processing device and method
CN112481266A (en) * 2020-12-17 2021-03-12 中国科学院深圳先进技术研究院 Gene element and using method thereof

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