BR112012015119A2 - "ácidos graxos de cadeia ramificada e produção biológica dos mesmos". - Google Patents

"ácidos graxos de cadeia ramificada e produção biológica dos mesmos".

Info

Publication number
BR112012015119A2
BR112012015119A2 BR112012015119A BR112012015119A BR112012015119A2 BR 112012015119 A2 BR112012015119 A2 BR 112012015119A2 BR 112012015119 A BR112012015119 A BR 112012015119A BR 112012015119 A BR112012015119 A BR 112012015119A BR 112012015119 A2 BR112012015119 A2 BR 112012015119A2
Authority
BR
Brazil
Prior art keywords
conversion
fatty acid
cell
oxobutanoate
chain fatty
Prior art date
Application number
BR112012015119A
Other languages
English (en)
Inventor
Richard Green Phillip
Winston Saunders Charles
Blair Cody David
Xu Jun
Sarosh Khambatta Zubin
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Publication of BR112012015119A2 publication Critical patent/BR112012015119A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04004—3-Methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (1.2.4.4), i.e. branched-chain-alpha-ketoacid dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0118—Beta-ketoacyl-acyl-carrier-protein synthase III (2.3.1.180)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01182—(R)-Citramalate synthase (2.3.1.182)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01019—Threonine ammonia-lyase (4.3.1.19)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"ácido graxos de cadeia ramificada e produção biológica dos mesmos" a presente invenção refere-se a um método para produzir ácido graxo de anteiso. o método compreende cultivar uma célula que compreende pelo menos um polinucleotídeo exógeno ou superexpresso que compreende uma sequência de ácido nucleico que codifica um polipeptídeo que catalisa pelo menos uma das seguintes reações: conversão de piruvato em citramalato; conversão de citramalato em citraconato; conversão de citraconato em b-metil-d-malato; conversão de b-metil-d-malato em 2-oxobutanoato; ou conversão de treonina em 2-oxobutanoato, em conndições que permitem a expressão do (s) polinucleotídeo (s) e produção de ácido graxo de anteiso. opcionalmente, a célula compreende adicionalmente pelo menos um polinucleotídeo exógeno ou superexpresso que compreende uma sequência de ácido nucleico que codifica um polipeptídeo que catalisa a conversão de 2-oxobutanoato em 2-aceto-2-hidróxi-butirato, a conversão de 2-aceto-2-hidróxi-butirato em 2,3-diidróxi-3-metilvalerato e/ou a conversão de 2,3-diidróxi-3-metivalerato em @-ceto-3-metilvalerato. uma célula que produz ácido graxo de anteiso e um método para o uso da célula para produzir ácido graxo de anteiso também são fornecidos.
BR112012015119A 2009-12-22 2010-12-21 "ácidos graxos de cadeia ramificada e produção biológica dos mesmos". BR112012015119A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28903909P 2009-12-22 2009-12-22
US12/972,822 US20110151526A1 (en) 2009-12-22 2010-12-20 Branched-Chain Fatty Acids And Biological Production Thereof
PCT/US2010/061544 WO2011087787A1 (en) 2009-12-22 2010-12-21 Branched-chain fatty acids and biological production thereof

Publications (1)

Publication Number Publication Date
BR112012015119A2 true BR112012015119A2 (pt) 2017-03-28

Family

ID=44151653

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012015119A BR112012015119A2 (pt) 2009-12-22 2010-12-21 "ácidos graxos de cadeia ramificada e produção biológica dos mesmos".

Country Status (10)

Country Link
US (1) US20110151526A1 (pt)
EP (1) EP2516650B1 (pt)
CN (1) CN103097538B (pt)
AU (1) AU2010341495A1 (pt)
BR (1) BR112012015119A2 (pt)
CA (1) CA2781730A1 (pt)
ES (1) ES2525150T3 (pt)
MX (1) MX2012007184A (pt)
WO (1) WO2011087787A1 (pt)
ZA (1) ZA201203767B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
EP3456814A3 (en) * 2011-01-14 2019-05-29 REG Life Sciences, LLC Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
MX366939B (es) 2012-04-02 2019-07-31 Reg Life Sciences Llc Producción mejorada de derivados de ácidos grasos.
US10119145B2 (en) * 2013-03-15 2018-11-06 Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet) Engineered organisms for production of novel lipids
KR101747542B1 (ko) * 2015-10-23 2017-06-15 씨제이제일제당 (주) L-이소루신 생산능을 가지는 코리네박테리움 속 미생물 및 이를 이용하여 l-이소루신을 생산하는 방법
WO2017165397A1 (en) 2016-03-22 2017-09-28 University Of Georgia Research Foundation, Inc. Genetically engineered microbes and methods for producing citramalate
CN109722405B (zh) * 2017-10-31 2023-06-23 创享(天津)生物科技发展有限公司 利用葡萄糖生产异丙基吡喃酮的重组大肠杆菌及用途
US20220243233A1 (en) * 2018-10-04 2022-08-04 Anna University Production of l-2-aminobutyrate from citramalate,citraconate or 2-oxobutanoate
CN110982742B (zh) * 2019-12-12 2021-10-22 石河子大学 一种耐盐促生菌wp-3及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020010934A (ko) * 1999-06-25 2002-02-06 추후제출 분지형의 실질적으로 불포화된 지방 알콜
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
WO2007136762A2 (en) 2006-05-19 2007-11-29 Ls9, Inc. Production of fatty acids and derivatives thereof
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
SG182157A1 (en) 2007-06-01 2012-07-30 Solazyme Inc Production of oil in microorganisms
BRPI0920010A2 (pt) 2008-10-28 2016-04-19 Ls9 Inc métodos para produzir álcool graxo e surfactante
US20100298612A1 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
ZA201203767B (en) 2014-10-29
CA2781730A1 (en) 2011-07-21
WO2011087787A1 (en) 2011-07-21
EP2516650A1 (en) 2012-10-31
CN103097538B (zh) 2015-06-03
ES2525150T3 (es) 2014-12-18
MX2012007184A (es) 2012-07-10
AU2010341495A1 (en) 2012-07-12
US20110151526A1 (en) 2011-06-23
CN103097538A (zh) 2013-05-08
EP2516650B1 (en) 2014-09-17

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]