AU2020102268A4 - Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof - Google Patents
Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof Download PDFInfo
- Publication number
- AU2020102268A4 AU2020102268A4 AU2020102268A AU2020102268A AU2020102268A4 AU 2020102268 A4 AU2020102268 A4 AU 2020102268A4 AU 2020102268 A AU2020102268 A AU 2020102268A AU 2020102268 A AU2020102268 A AU 2020102268A AU 2020102268 A4 AU2020102268 A4 AU 2020102268A4
- Authority
- AU
- Australia
- Prior art keywords
- culture medium
- bamboo chopsticks
- parts
- edible fungus
- auxiliary materials
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G11/00—Sterilising soil by steam
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses an edible fungus culture medium based on disposable bamboo
chopsticks and preparation technology and application thereof, it relates to the field of culture
medium preparation. The culture medium comprises the following components: 20-30 parts
of peat soil, 35-45 parts of sawdust, 7-9 parts of bran, 1-2 parts of gypsum powder, 1-2 parts
of lime powder, 0.1-0.6 part of vitamin B, 1-2 parts of calcium superphosphate and 40-55
parts of water; The ratio of auxiliary materials to bamboo chopsticks is 1 part of auxiliary
materials contains 120-140 bamboo chopsticks. The preparation technology of that culture
medium is simple, and when apply it to the cultivation of edible fungi, it can improve quality
and production efficiency of the strains, shortening the production cycle of the strains
remarkably and improving the economic benefit greatly. Thus, the culture medium of the
present invention has a good potential of popularization and application.
Description
PATENTS ACT 1990
Edible fungus culture medium based on disposable bamboo chopsticks and preparation
method and application thereof
The invention is described in the following statement:-
Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof
The invention relates to the technical field of strain cultivation, in particular to an edible fungus culture medium based on disposable bamboo chopsticks and a preparation method and application thereof.
Edible fungi production is pollution-free, pure natural, recyclable, renewable and environmentally friendly. Edible fungi has become the sixth largest agricultural product after grain, cotton, oil, fruit and vegetables in the traditional agricultural production field; In the process of agricultural modernization, edible fungi industry has become the vanguard and main force of modern agricultural development; Hou Zhengxiang of the Development Research Center of the State Council said that edible fungi as a sunrise industry will soon surpass China's grain, cotton and oil industry as well as China's automobile industry.
In the new century, the production technology of edible fungi in China is developing with each passing days, and having breakthroughs in many technical fields, which let China become a well-developed market to edible fungi with powerful demand, however, due to the long production cycle, such demand is difficult to meet.
At the same time, disposable bamboo chopsticks are widely used in China, and most of them become waste, causing pollution and waste. The main component of bamboo chopsticks is cellulose, which is a suitable nutrient medium for the growth of edible fungi and can be used as a carrier for cultivating edible fungi.
Therefore, it brings a great practical significance to fungi cultivation by providing an edible fungus culture medium based on disposable bamboo chopsticks that can shorten its production cycle.
The purpose of the invention is to provide an edible fungus culture medium based on disposable bamboo chopsticks and a preparation method and application thereof, so as to solve the problems existing in the prior art.
The invention provides an edible fungus culture medium based on disposable bamboo chopsticks, which comprises auxiliary materials and bamboo chopsticks, wherein the auxiliary materials comprise the following raw materials in parts by mass: 20-30 parts of peat soil, 35-45 parts of sawdust, 7-9 parts of bran, 1-2 parts of gypsum powder, 1-2 parts of lime powder, 0.1-0.6 part of vitamin B and 1-2 parts of calcium superphosphate. Among them, the ratio of auxiliary materials to bamboo chopsticks is that one part of auxiliary materials contains 120-140 bamboo chopsticks.
The invention also provides a preparation method of the edible fungus culture medium based on disposable bamboo chopsticks, which comprises the following steps:
(1) The bamboo chopsticks are cleaned to absorb water, and then the bamboo chopsticks are roughened to make their surface rough;
(2) Stirring peat soil, sawdust, bran, gypsum powder, lime powder, vitamin B, calcium superphosphate and water uniformly to prepare culture medium auxiliary materials;
(3) Arranging the bamboo chopsticks processed in the step (1) in a culture medium auxiliary material, and covering gaps and surfaces of the bamboo chopsticks with the culture medium auxiliary material to obtain a preliminarily processed culture medium;
(4) Putting the culture medium preliminarily processed in step (3) into a steam sterilization pot for sterilization, and cooling it to obtain the edible fungus culture medium based on disposable bamboo chopsticks.
Further, in Step(4), the condition of the sterilization is 0.103MPa and 121°C for 40-50 minutes.
The invention also provides the application of the edible fungus culture medium based on disposable bamboo chopsticks in edible fungus strain culture.
Further, breaking the fermented edible fungus liquid hyphae balls, diluting it by 300-500 times sterile water to obtain liquid strains, and inoculating the liquid strains on the edible fungus culture medium based on disposable bamboo chopsticks according to the inoculation amount of 20-25ml, and culturing the culture medium at 26°C for 3-10 days.
The invention discloses the following technical effects:
In the technical research and production application of edible fungi, the general sterilization conditions of solid substrates (bamboo chopsticks, etc.) are 0.138MPa, 126°C, and sterilization is executed for about 120 minutes, which is time-consuming and energy-consuming, and causes the loss of nutrient components of bamboo chopsticks substrate. However, the sterilization parameters of the present invention not only achieves a good sterilization effect, but also lowers the energy waste of sterilization and reduces the loss of nutrient components of bamboo chopsticks substrate. The liquid hyphae can quickly spread on the surface of bamboo chopsticks and grow inward, making the growth of edible fungi hyphae to be in a dominant state, hence inhibiting the growth of miscellaneous bacteria. Therefore, by reducing sterilization parameters properly will not affect the normal growth of edible fungi hyphae on the surface of bamboo chopsticks, On the other hand, due to the sterilization parameters of the invention, the treatment temperature is reduced and the treatment time is shortened, thereby reducing the nutrient components loss and lower the energy use.
The inoculation amount directly affects the germination and growth effect of hyphae, if the amount is small, the hyphae grow slowly, and the long growth cycle can easily cause the pollution of miscellaneous bacteria. If the amount of inoculation is large, it causes waste and compels a high requirement for sanitary environment, refrigeration and ventilation equipment, which does not suit the factory with weak technical force and insufficient technical personnel, and it especially does not suit individual farmers. More importantly, when the amount of inoculation is large, it will form a layer of bacterial mucosa on the surface of bamboo chopsticks that blocks the entry of fresh air, causing growth failure of internal hyphae, ultimately leading to the pollution of miscellaneous bacteria and increasing the use risk of liquid strains. In this invention, the inoculation amount is adjusted. The fermented edible fungus liquid hypha balls is broken and diluted by 300-500 times with sterile water to obtain liquid strains, then inoculated the strains on the prepared culture medium according to the inoculation amount of 20-25m, by which it can reduce the waste of resources and ensure the germination effect, meanwhile inhibit a layer of bacterial mucosa on the surface of bamboo chopsticks that blocks the entry of fresh air, guaranteeing the oxygen supply for hyphae to grow properly.
Peat soil and disposable bamboo chopsticks are also used in the invention, and peat soil stems from many years of naturally fermentation, it has good maturity and strong water holding capacity and suitable fertility, besides it is abundant in China with low purchasing price. Peat soil can improve the fertility and lower the cost of culture medium. The main component of bamboo chopsticks is cellulose, which is a suitable nutrient substrate for the growth of edible fungi. The invention also reduces the environmental pollution caused by disposable bamboo chopsticks.
The culture medium of the invention is also added with vitamin B, which can promote the good development of edible fungi hypha; The culture medium provided by the invention can improve the quality and production efficiency of strains, shorten the period of strain production, and greatly improve the economic benefit, overall, it has a great potential of popularization and application.
Various embodiments of the present invention will now be described in detail, which should not be regarded as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics and embodiments of the present invention.
It should be understood that the terms described in the present invention are only for describing specific embodiments, and are not intended to limit the present invention. Furthermore, as for the numerical range in the present invention, it should be understood that every intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Intermediate values within any stated value or stated range and every smaller range between any other stated value or intermediate values within the stated range are also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.
Unless otherwise stated, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art to which the present invention relates. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein may be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe methods and/or materials related to the documents. In case of conflict with any incorporated documents, the contents of this specification shall prevail.
Without departing from the scope or spirit of the invention, it is obvious to those skilled in the art that many modifications and changes can be made to the specific embodiments of the specification of the invention. Other embodiments derived from the description of the present invention will be apparent to the skilled person. The specification and examples of this application are only exemplary.
As used herein, "including", "including", "having", "containing", etc. are all open terms, which means including but not limited to.
Embodiment 1
The edible fungus culture medium in this embodiment comprises auxiliary materials and bamboo chopsticks, wherein the auxiliary materials comprise the following components in parts by mass: parts of peat soil, 40 parts of sawdust, 8 parts of bran, 1 part of gypsum powder, 1 part of lime powder, 0.4 part of vitamin b, 1.5 parts of calcium superphosphate and 50 parts of water; Among them, the ratio of auxiliary material to bamboo chopsticks is 1:140
Preparation and inoculation of edible fungi culture medium;
(1) Cutting waste disposable bamboo chopsticks into strips with a diameter of 0.5 cm and a length of 7.5cm, cleaning the bamboo chopsticks, and making them absorb water, then roughening the bamboo chopsticks to make their surfaces rough
(2) Stirring peat soil, sawdust, bran, gypsum powder, lime powder, vitamin B, calcium superphosphate and water uniformly to prepare culture medium auxiliary materials;
(3) Arranging the bamboo chopsticks processed in the step (1) in a culture medium auxiliary material, covering gaps and surfaces of the bamboo chopsticks with the culture medium auxiliary material to obtain a preliminarily processed culture medium;
(4) Putting the culture medium preliminarily treated in the step (3) into a steam sterilization pot for sterilization at the temperature of 0.103MPa and 121°C for 40 minutes, and cooling it to obtain the edible fungus culture medium based on disposable bamboo chopsticks.
(5) Breaking fermented edible fungus liquid hypha ball, diluting them by 300 times sterile water to obtain liquid strains, inoculating liquid hypha ofpleurotus eryngii on the edible fungus culture medium based on disposable bamboo chopsticks according to the inoculation amount of 20ml, and cultured under condition of 26°C for 3 days, making the surface of the bamboo chopsticks covered with hyphae, and keep culturing it for 5 days, so that the interior of the bamboo chopsticks is covered with hyphae.
The hyphae cultivated is thick and dense, and the production cycle of strain was 23 days.
Embodiment 2
The edible fungus culture medium in this embodiment comprises auxiliary materials and bamboo chopsticks, wherein the auxiliary materials comprise the following components in parts by mass:
parts of peat soil, 45 parts of sawdust, 9 parts of bran, 2 parts of gypsum powder, 2 parts of lime powder, 0.1 part of vitamin b, 1 part of calcium superphosphate and 40 parts of water; the ratio of auxiliary material to bamboo chopsticks is 1:120
Preparation and inoculation of edible fungi culture medium;
(1) Cutting waste disposable bamboo chopsticks into strips with a diameter of 0.5 cm and a length of 7.5cm, cleaning the bamboo chopsticks, and making them absorb water, then roughening the bamboo chopsticks to make their surfaces rough
(2) Stirring peat soil, sawdust, bran, gypsum powder, lime powder, vitamin B, calcium superphosphate and water uniformly to prepare culture medium auxiliary materials;
(3) Arranging the bamboo chopsticks processed in the step (1) in a culture medium auxiliary material, covering gaps and surfaces of the bamboo chopsticks with the culture medium auxiliary material to obtain a preliminarily processed culture medium;
(4) Putting the culture medium preliminarily treated in the step (3) into a steam sterilization pot for sterilization at the temperature of 0.103MPa and 121°C for 50 minutes, and cooling it to obtain the edible fungus culture medium based on disposable bamboo chopsticks.
(5) Breaking fermented edible fungus liquid hypha ball, diluting them by 400 times sterile water to obtain liquid strains, inoculating liquid hypha ofpleurotus eryngii on the edible fungus culture medium based on disposable bamboo chopsticks according to the inoculation amount of 22ml, and cultured under condition of 26°C for 4 days, making the surface of the bamboo chopsticks covered with hyphae, and keep culturing it for 4 days, so that the interior of the bamboo chopsticks is covered with hyphae.
The hyphae cultivated is thick and dense, and the production cycle of strain was 20 days.
Embodiment 3
The edible fungus culture medium in this embodiment comprises auxiliary materials and bamboo chopsticks, wherein the auxiliary materials comprise the following components in parts by mass:
parts of peat soil, 35 parts of sawdust, 7 parts of bran, 1 part of gypsum powder, 1 part of lime powder, 0.6 part of vitamin B, 2 parts of calcium superphosphate and 55 parts of water; the ratio of auxiliary material to bamboo chopsticks is 1:140
Preparation and inoculation of edible fungi culture medium;
(1) Cutting waste disposable bamboo chopsticks into strips with a diameter of 0.5 cm and a length of 7.5cm, cleaning the bamboo chopsticks, and making them absorb water, then roughening the bamboo chopsticks to make their surfaces rough
(2) Stirring peat soil, sawdust, bran, gypsum powder, lime powder, vitamin B, calcium superphosphate and water uniformly to prepare culture medium auxiliary materials;
(3) Arranging the bamboo chopsticks processed in the step (1) in a culture medium auxiliary material, covering gaps and surfaces of the bamboo chopsticks with the culture medium auxiliary material to obtain a preliminarily processed culture medium;
(4) Putting the culture medium preliminarily treated in the step (3) into a steam sterilization pot for sterilization at the temperature of 0.103MPa and 121°C for 45 minutes, and cooling it to obtain the edible fungus culture medium based on disposable bamboo chopsticks.
(5) Breaking fermented edible fungus liquid hypha ball, diluting them by 500 times sterile water to obtain liquid strains, inoculating liquid hypha ofpleurotus eryngii on the edible fungus culture medium based on disposable bamboo chopsticks according to the inoculation amount of 25ml, and cultured under condition of 26°C for 5 days, making the surface of the bamboo chopsticks covered with hyphae, and keep culturing it for 3 days, so that the interior of the bamboo chopsticks is covered with hyphae.
The hyphae cultivated is thick and dense, and the production cycle of strain was 25 days.
Comparative Embodiment 1
Different from Example 1, if the sterilization conditions were 0.138MPa, 126°C and 120 minutes, the prepared hyphae are sparse and in poor state.
Comparative Embodiment 2
Different from Example 1, if the inoculation amount was 30ml, the prepared hyphae are sparse and in poor state.
The above embodiments only describe the preferred mode of the invention, but they do not limit the scope of the invention. On the premise of not deviating from the designing intent of the present invention, any possible various modifications and improvements that could be made on this present invention by those skilled in the prior art shall fall within the protection scope determined by the claims of the invention.
Claims (6)
1. An edible fungus culture medium based on disposable bamboo chopsticks, which is characterized in that the edible fungus culture medium comprises auxiliary materials and bamboo chopsticks, wherein the auxiliary materials comprise the following raw materials in parts by mass: 20-30 parts of peat soil, 35-45 parts of sawdust, 7-9 parts of bran, 1-2 parts of gypsum powder, 1-2 parts of lime powder, 0.1-0.6 part of vitamin b, and 1-2 parts of vitamin b.Among them, the ratio of auxiliary materials to bamboo chopsticks is that one part of auxiliary materials contains 120-140 bamboo chopsticks.
2. According to claim 1, the edible fungus culture medium based on disposable bamboo chopsticks is characterized in that the edible fungus culture medium comprises auxiliary materials and bamboo chopsticks, wherein the auxiliary materials comprise the following raw materials in parts by mass: 25 parts of peat soil, 40 parts of sawdust, 8 parts of bran, 1 part of gypsum powder, 1 part of lime powder, 0.4 part of vitamin B, 1.5 parts of calcium superphosphate and 50 parts of water; Among them, the ratio of auxiliary materials to bamboo chopsticks is: 1 part of auxiliary materials contains 140 bamboo chopsticks.
3. According to either one of claims 1-2, the preparation technology of edible fungi culture medium based on disposable bamboo chopsticks is characterized by comprising the following steps:
(1) Cleaning the bamboo chopsticks, and making them absorb with water, then roughen their surfaces.
(2) Stirring peat soil, sawdust, bran, gypsum powder, lime powder, vitamin B, calcium superphosphate and water uniformly to prepare culture medium auxiliary materials;
(3) Arranging the bamboo chopsticks processed in the step (1) in a culture medium auxiliary material, and covering gaps and surfaces of the bamboo chopsticks with the culture medium auxiliary material to obtain a preliminarily processed culture medium;
(4) Putting the culture medium preliminarily processed in step (3) into a steam sterilization pot for sterilization, and cooling it to obtain the edible fungus culture medium based on disposable bamboo chopsticks.
4. According to claim 3, the preparation technology of edible fungus culture medium based on disposable bamboo chopsticks is characterized in that in step (4), the sterilization is carried out under condition of 0.103MPa and 121°C for 40-50 minutes.
5. According to either one of claims 1-2, the edible fungus culture medium based on disposable bamboo chopsticks is applied to the cultivation of edible fungus strains.
6. According to claim 5, the application of culture is characterized in breaking the fermented edible fungus liquid hyphae balls, diluting them by 300-500 times sterile water to obtain liquid strains, and inoculating the liquid strains on the edible fungus culture medium based on disposable bamboo chopsticks according to the inoculation amount of -25ml, and culturing the culture medium at 26°C for 3-10 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020102268A AU2020102268A4 (en) | 2020-09-15 | 2020-09-15 | Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020102268A AU2020102268A4 (en) | 2020-09-15 | 2020-09-15 | Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020102268A4 true AU2020102268A4 (en) | 2020-10-29 |
Family
ID=72926629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020102268A Ceased AU2020102268A4 (en) | 2020-09-15 | 2020-09-15 | Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2020102268A4 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109609391A (en) * | 2019-02-15 | 2019-04-12 | 福州大学 | A kind of quantitative inoculation method of mycelium pellet |
| CN113170700A (en) * | 2021-04-26 | 2021-07-27 | 江苏裕灌现代农业科技有限公司 | Fermentation material for producing agaricus bisporus and preparation method thereof |
| CN113597974A (en) * | 2021-08-26 | 2021-11-05 | 重庆市农业科学院 | Edible fungus renewable culture medium, preparation method and application |
-
2020
- 2020-09-15 AU AU2020102268A patent/AU2020102268A4/en not_active Ceased
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109609391A (en) * | 2019-02-15 | 2019-04-12 | 福州大学 | A kind of quantitative inoculation method of mycelium pellet |
| CN113170700A (en) * | 2021-04-26 | 2021-07-27 | 江苏裕灌现代农业科技有限公司 | Fermentation material for producing agaricus bisporus and preparation method thereof |
| CN113170700B (en) * | 2021-04-26 | 2023-07-25 | 江苏裕灌现代农业科技有限公司 | Fermentation material for producing agaricus bisporus and preparation method thereof |
| CN113597974A (en) * | 2021-08-26 | 2021-11-05 | 重庆市农业科学院 | Edible fungus renewable culture medium, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020102268A4 (en) | Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof | |
| CN103483064B (en) | Culture medium using waste pomegranate twigs as matrix and preparation method thereof | |
| CN111073839B (en) | Siam bacillus, microbial inoculum and application thereof | |
| CN103539535A (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN106316693A (en) | Biological humic acid fertilizer and preparing method thereof | |
| CN103992184A (en) | Bio-organic fertilizer | |
| CN109796289A (en) | It is a kind of using oil-tea camellia husks as bio-fertilizer of raw material and preparation method thereof | |
| CN108703011B (en) | Method for reducing trichoderma generation and improving yield in agaricus bisporus cultivation | |
| US20220033762A1 (en) | Penicillium oxalicum SDF-25 strain and application thereof | |
| CN103250529B (en) | Method of cultivating chrysanthemum by probiotic nutrients | |
| CN102617208A (en) | Edible fungus residue organic fertilizer raw materials and preparation method thereof | |
| CN109983951A (en) | A kind of seedling culture with biomass disk and its preparation method and application | |
| CN110862939A (en) | Special microbial agent for apple trees and preparation method thereof | |
| CN108432548B (en) | Formula and preparation method of morchella esculenta cultivar by using mushroom bran | |
| CN103288499B (en) | A kind of ecological organic pot for growing seedlings and preparation method thereof | |
| CN101288359B (en) | Method for stopping clover seeds dormancy in hard seeds | |
| CN101451107B (en) | Method for large scale preparing Gliocladium chlamydospore | |
| CN107467075B (en) | Application of bacillus pumilus as rice growth promoter | |
| CN111154661B (en) | Complex microbial inoculant and application thereof | |
| CN107974423B (en) | Soil biological activator and preparation method thereof | |
| CN105647812B (en) | One plant of schizophyllum commune bacterial strain and its application in Pu'er tea production | |
| CN108157060B (en) | Hericium erinaceus culture medium, preparation method thereof and cultivation method of hericium erinaceus | |
| CN108834763B (en) | Pretreatment method of pine sawdust as edible fungus cultivation material | |
| CN112493054A (en) | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof | |
| CN111615995A (en) | Culture medium for liquid fermentation of pleurotus eryngii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |