AU2003288610B2 - An athermal process for the concentration of Garcinia extract - Google Patents
An athermal process for the concentration of Garcinia extract Download PDFInfo
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- AU2003288610B2 AU2003288610B2 AU2003288610A AU2003288610A AU2003288610B2 AU 2003288610 B2 AU2003288610 B2 AU 2003288610B2 AU 2003288610 A AU2003288610 A AU 2003288610A AU 2003288610 A AU2003288610 A AU 2003288610A AU 2003288610 B2 AU2003288610 B2 AU 2003288610B2
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- garcinia
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- 239000000284 extract Substances 0.000 title claims description 46
- 238000000034 method Methods 0.000 title claims description 42
- 241000593508 Garcinia Species 0.000 title claims description 26
- 235000000885 Garcinia xanthochymus Nutrition 0.000 title claims description 26
- 239000012528 membrane Substances 0.000 claims description 40
- 238000004821 distillation Methods 0.000 claims description 13
- 239000002357 osmotic agent Substances 0.000 claims description 13
- 230000003204 osmotic effect Effects 0.000 claims description 13
- 241000283690 Bos taurus Species 0.000 claims description 10
- 235000013399 edible fruits Nutrition 0.000 claims description 10
- 230000002209 hydrophobic effect Effects 0.000 claims description 10
- 241001051053 Garcinia cowa Species 0.000 claims description 9
- 241001627228 Garcinia pedunculata Species 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 8
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 8
- 230000001965 increasing effect Effects 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 7
- 230000002572 peristaltic effect Effects 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical group [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 229910000831 Steel Inorganic materials 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 239000010959 steel Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 150000002596 lactones Chemical class 0.000 claims description 3
- 235000011869 dried fruits Nutrition 0.000 claims description 2
- 230000000050 nutritive effect Effects 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims 2
- 235000013305 food Nutrition 0.000 description 5
- 239000004743 Polypropylene Substances 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- -1 polypropylene Polymers 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- ZMJBYMUCKBYSCP-UHFFFAOYSA-N Hydroxycitric acid Chemical compound OC(=O)C(O)C(O)(C(O)=O)CC(O)=O ZMJBYMUCKBYSCP-UHFFFAOYSA-N 0.000 description 2
- 159000000007 calcium salts Chemical class 0.000 description 2
- 229940089491 hydroxycitric acid Drugs 0.000 description 2
- 230000003685 thermal hair damage Effects 0.000 description 2
- VYYWVGGAJXBBCA-YIRLFHOGSA-K tripotassium;(1s,2s)-1,2-dihydroxypropane-1,2,3-tricarboxylate Chemical compound [K+].[K+].[K+].[O-]C(=O)[C@@H](O)[C@](O)(C([O-])=O)CC([O-])=O VYYWVGGAJXBBCA-YIRLFHOGSA-K 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 230000004580 weight loss Effects 0.000 description 2
- GMVPRGQOIOIIMI-DODZYUBVSA-N 7-[(1R,2R,3R)-3-hydroxy-2-[(3S)-3-hydroxyoct-1-enyl]-5-oxocyclopentyl]heptanoic acid Chemical compound CCCCC[C@H](O)C=C[C@H]1[C@H](O)CC(=O)[C@@H]1CCCCCCC(O)=O GMVPRGQOIOIIMI-DODZYUBVSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000546193 Clusiaceae Species 0.000 description 1
- 244000245602 Garcinia atroviridis Species 0.000 description 1
- 241000173371 Garcinia indica Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 235000019631 acid taste sensations Nutrition 0.000 description 1
- 125000005233 alkylalcohol group Chemical group 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000005961 cardioprotection Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004136 fatty acid synthesis Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229940124600 folk medicine Drugs 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004132 lipogenesis Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000037221 weight management Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Obesity (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Child & Adolescent Psychology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicines Containing Plant Substances (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Extraction Or Liquid Replacement (AREA)
Description
WO 2005/070863 1 PCT/IB2003/006105 AN ATHERMAL PROCESS FOR THE CONCENTRATION OF GARCINIA EXTRACT Field of the invention: The present invention relates to an athermal process for the concentration of Garcinia 5 extract. The present invention particularly relates to a process of obtaining concentrated Garcinia extract by using osmotic membrane distillation (OMD). Background of the invention: As dietary supplement, (-)-Hydroxcitic acid (HCA) is an effective agent to any weight management program. Allison et al. (Crit.Rev.Food Sci.Nutr. 2001, 41,1-28) has reviewed 10 the use of HCA as one of the alternative treatments for weight loss. The derivatives of HCA have been incorporated into many pharmaceutical preparations in combination with other ingredients for the purpose of enhancing weight loss, cardioprotection, correcting the conditions of lipid abnormalities and endurance(Jena et al., 2002, J. Agric. Food Chemistry, 50,10-22). So far HCA has been found in the fruits of certain species of 15 Garcinia, which includes G. cambogia, G. indica, G.atroviridis and G. cowa (Lewis, Y.S. 1969, Methods in enzymology, 13, 613-623; Jena et al., 2002, J. Agric. Food Chemistry, 50,10-22). The chemistry and biochemistry of HCA has been discussed recently (Jena et al., 2002, J. Agric. Food Chemistry, 50,10-22). During extensive animal studies, HCA has been proven to effectively curb appetite, suppress food intake, increase the rates of hepatic 20 glycogen synthesis, reduce fatty acid synthesis and lipogenesis and decrease body-weight gain. Garcinia (Family: Guttiferae) is a large genus of polygamous trees or shrubs, distributed in the tropical Asia, Africa and Polynesia. It consists of 180 species, out of which about 30 species are found in India. G. pedunculata and G. cowa are grown in Northeastern parts of 25 India and Andaman Islands. In Assam G. cowa is often cultivated in homesteads for its acid fruits (The Wealth of India, 1956). The fruits from both the species of Garcinia are not palatable due to their strong acid taste. In Assam the sun dried slices of the fruits are used for culinary purposes and as folk medicine. Reference may be made to the commercial samples of Garcinia canibogia extracts, where 30 the HCA is present as its calcium salt (Sawada, et al, 1997, Nihon yukagaka kaishi, vol,1467-1474). But the excess calcium reduces the solubility and subsequently bioavailability when it is compared to the liquid extract.
WO 2005/070863 2 PCT/IB2003/006105 Another reference may be made to the Ashok kumar., Ravindranath, B., and Balasubramamanvam (US patent # 656314, 1996 ). This process involves water extraction, followed by passing through ion exchange resins and decolorisation by using activated charcoal and finally concentration by using vacuum evaporation. The main drawback of 5 this method is the thermal process and also it involves too many unit operations for obtaining the final product. Reference may be made to Majeed et al. (Majeed, M., Badmaev, V and Rajendran, R. US Patent. 5783603, 1998), wherein the preparation of potassium hydroxycitrate from Garcinia fruit was reported. It involves the extraction of Garcinia fruit using alkyl alcohol, 10 the extract was treated with potassium hydroxide and refluxed to form potassium hydroxycitrate precipitate. The main drawback of this method is the potassium salt is hygroscopic. Further, HCA is not available in natural state, limiting its bioavailability and versatile applicability for pharmaceutical purposes. It may be noted that all these references are mentioned above HCA derivatives 15 preparations. But, there is no report on non-thermal process for concentrating of HCA and also in its native (not as derivatives) form. Objects of the invention: The main object of the present invention is to develop a process for the concentrated Garcinia extract from G. pedunculata / G.cowa in liquid form. 20 Another object of present invention is to develop a non-thermal process for the concentration of Garcinia extract. Still another object of the present invention is to develop a process for the concentrating of Garcinia extract using osmotic membrane distillation (OMD). Yet another object of present invention is to provide a simple single step and efficient 25 economical process for the concentration of HCA, wher it is present in its native form. Detailed description of the invention: Accordingly, the present invention provides an athermal process for the concentrating of Garcinia extract, which comprises (a) collecting the dried fruit rinds may be effected from the species of Garcinia. 30 (b) cutting the rinds of G. pedunculata /G.cowa manually to a size of 3x9mm to 6x 14mm. (c) extracting may be effected with de-ionized water at a volume ratio of t:4 for a period of 15-35 min at 110-130'C.
WO 2005/070863 3 PCT/IB2003/006105 (d) filtering the above extract may be effected by filter cloth. (e) concentrating the HCA by osmotic membrane distillation (OMD) in a co current flat membrane module. (f) placing a hydrophobic membrane between two steel frames SS316 of the 5 module with suitable spaces. (g) circulating the extract at a flow rate of 100-150 ml/min on the one side of the membrane using a multi-stage peristaltic pump. (h) hydrophobic membrane osmotic agent (OA) on the other side of the membrane using a multi-stage peristaltic pump. 10 (i) carrying out OMD for about 4-6 hrs till the extract was concentrated in the feed tank. In an embodiment of the present invention, the HCA concentration may be obtained from the aqueous extract of G. pedunculata and G.cowa. In an another embodiment of the present invention, concentrated Garcinia extract may be 15 obtained by using an athermal membrane process namely, Osmotic membrane distillation (OMD) at ambient temperature (25± 1C) and pressure (latm). Still another embodiment of present invention, the HCA content of the concentrated Garcinia extract is in the range of 33-37% estimated by HPLC method. Yet in another embodiment of present invention the Garcinia extract was concentrated by a 20 simple single step process where the HCA content was increased from 4-6 fold and HCA is present in the native form (not as derivative) with out formation of lactone, increasing it commercial and nutritive values. In the present invention, rinds of G. pedunculata / Gcowa were cut into small pieces and extracted with de-ionized water. The above extract was filtered through filter cloth. 25 Concentrating the HCA by osmotic membrane distillation in a co-current flat membrane module. The membrane module consisted of two steel frames SS316 between which a hydrophobic membrane was placed. The entire filtrate was circulated on the one side of the OMD membrane and on the other side, an osmotic agent (OA) was circulated using a multi-stage peristaltic pump. After desired time the extract was concentrated in the feed 30 tank. The purity of the preparation was analyzed by HPLC as described by Jayaprakasha, G.K. and Sakariah, K.K. (J. Liquid Chromatography & Related Technologies, 23, 915-923, 2000). Concentrated hydroxycitric acid (0.1 g) was dissolved in water and made up to WO 2005/070863 4 PCT/IB2003/006105 100ml with water and filtered. The high performance liquid chromatographic system consisted of a Hewlett Packard HPLC model HP 1100 Series (Hewlett-Packard, CA, USA), fitted with a Waters p-Bondapack" m (Waters Corporation, Milford, MA, USA) C 1 8 column (250 x 4.6 mm I.D). The injection system (Rheodyne) used was 20 pl sample loop. 5 Detection was done by a HP 1100 series variable wavelength detector at wavelength of 210 nm. The elution was carried out with 8 mM sulphuric acid and flow rate was 1.0 ml/min under isocratic condition. A known volume (10il) of the samples was injected on to the HPLC and the concentration of HCA was obtained directly from the peak area and by application of the dilution factor. The HCA concentration of the sample was expressed as 10 g/100 g of sample. The purity of hydroxycitric acid was 33-35 % (w/w). The important aspects of the invention are: 1. Concentrated Garcinia extract is obtained by athermal membrane process. 2. This is a single step process for obtaining the concentrated Garcinia extract. 3. In the present process there is no phase change. 15 4. This process is operated at ambient temperature and pressure so that no thermal damage of the product. 5. HCA content was increased from 4-6 fold. 4. There is no lactone formation during this process. The following examples are given by way of illustration of the present invention and 20 should not be construed to limit the scope of the present invention. Example-i Fruit rinds of G. pedunculata in 500g quantity were cut into small pieces and extracted with 1.5 liters of de-ionized water for a period of 20 min at 120'C. The above extract was filtered through filter cloth. The extract was taken and subjected to concentration by 25 osmotic membrane distillation in a co-current flat membrane module using micro porous hydrophobic polypropylene membrane of 0.05 im at a flow rate of 100 ml/min. The module was operated in co-current mode using saturated calcium chloride as osmotic agent. The concentration was carried out till the volume of the extract was reduced to 1/5" of the original volume. The concentration of HCA had increased from 6 to 62*Brix. The 30 HCA content was determined by HPLC method and the acid was found to be 33.58% from an initial content of 5.09 %. (6 fold enhancement). Example-2 Fruit rinds of G. cowa in 10OOg quantity were cut into small pieces and extracted with 3 5 liters of de-ionized water for a period of 30 min at 130 psi using autoclaving. The extract was filtered through filter cloth. The extract was taken and subjected to concentration by osmotic membrane distillation in a co-current flat membrane module using micro porous hydrophobic polypropylene membrane of 0. 05, um at a flow rate of s 130 ml/min. The module was operated in co-current mode using saturated calcium chloride as osmotic agent. The concentration was carried out till the volume of the extract was reduced to 1 /5th of the original volume. The concentration of HCA had increased from 6 to 62*Brix. The HCA content was determined by HPLC method and the acid was found to be 35.5% from an initial content of 6%. (7 fold enhancement). 10 Example-3 Fruit rinds of G. pedunculata in 100gm quantity were cut into small pieces and extracting with liter of de-ionized water for a period of 50 min at 1200C using autoclaving. The extract was filtered through filter cloth. The extract was taken and subjected to concentration by osmotic membrane distillation in a co-current flat 15 membrane module using micro porous hydrophobic polypropylene membrane of 0. 05pm at a flow rate of 150 ml/min. The module was operated in co-current mode using saturated calcium chloride as osmotic agent. The concentration was carried out till the volume of the extract was reduced to 1/5two the original volume. The concentration of HCA had increased from 6 to 62 0 Brix. The HCA content was determined by HPLC 20 method and the acid was found to be 34.5% from an initial content of 5%. (6 fold enhancement). The main advantages of the present invention are 1. This is a simple and single step process for obtaining the concentrated free HCA. 2. This concentrated Garcinia extract is in its native form without being any of its 25 derivatives like sodium, potassium and calcium salts. Hence it will have better bioavailability. 3. This product does not undergo any thermal damage since the process is athermal, therby the product is suitable for food/therapeutic applications. 4. This process is simpler and easy to scale-up. 30 In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word "comprise" or variations such as "comprises" or "comprising" is used in an inclusive sense, i.e. to specify the presence of the stated features but not to 35 preclude the presence or addition of further features in various embodiments of the invention. 22870041 (GHMatters) 27/05/10 6 It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art, in Australia or any other country. 22870041 (GHMatters) 27/05/10
Claims (12)
1. An athermal process for concentrating Garcinia extract, which comprises 5 a) collecting and cutting dried rinds of a fruit selected from Garcinia sp, G. pedunculata and G. cowa, b) extracting the cut rinds with de-ionized water at a volume ratio of 1:4 for a period of 20-30 min at 11 50C-1 30 0 C to obtain an extract, c) filtering the extract to obtain a particle free extract, and 10 d) subjecting the particle free extract to osmotic membrane distillation in a co- current mode in the presence of an osmotic agent until the extract reduced to 1 / 5 th of its original volume.
2. The process as claimed in claim 1 further comprising the step of is obtaining hydrocitric acid from the concentrated extract of step (d).
3. The process as claimed in claim 1 wherein the osmotic membrane distillation is carried out in a co-current flat membrane module in which a hydrophobic membrane is placed between two SS316 steel frames located in a spaced apart 20 relationship.
4. The process as claimed in claim 1 wherein the extract is circulated at a flow rate of 100- 150 ml/min on one side of the membrane using a multi-stage peristaltic pump. 25
5. The process as claimed in claim 1 wherein a hydrophobic membrane osmotic agent (OA) is placed on the other side of the membrane using a multi-stage peristaltic pump. 30
6. The process as claimed in claim 1 wherein the osmotic agent is saturated calcium chloride.
7. The process as claimed in claim 1 wherein the osmotic membrane distillation is carried out at ambient temperature of 25± 1*C and pressure of 1 atm. 35
8. The process as claimed in claim 1 wherein the osmotic membrane distillation is carried on for about 4-6 hrs till the extract was concentrated in a feed 22870041 (GHMatters) 27105/10 8 tank.
9. The process as claimed in claim 1 wherein free hydrocitric acid content in the concentrated extract of step d) is in the range 33-35 % estimated by HPLC 5 method.
10. The process as claimed in claim 1 wherein hydrocitric acid content was increased from 4-6 fold and HCA is present in the native form (not as derivative) with out formation of lactone, increasing it commercial and nutritive values. 10
11. An athermal process for concentration of Garcinia extract comprising the steps of: a) collecting the dried fruit rinds may be effected from the species of Garcinia 15 b) cutting the rinds of G. pedunculatu / G. cowa manually to a size of 3x9mm to 6x 14mm c) extracting HCA with de-ionized water at a volume ratio of 1:4 for a period of 15-35 min at 110-1300C. d) filtering the above extract may be effected by filter cloth 20 e) concentrating the HCA by osmotic membrane distillation (OMD) in a co-current flat membrane module f) placing a hydrophobic membrane between two steel frames SS316 of the module with suitable spaces g) circulating the extract at a flow rate of 100-150 ml/min on the one side 25 of the membrane using a multi-stage peristaltic pump h) hydrophobic membrane osmotic agent (OA) on the other side of the membrane using a multi-stage peristaltic pump i) carrying out OMD for about 4-6 hrs till the extract was concentrated in the feed tank. 30
12. An athermal process for the concentration of Garcinia extract substantially as hereinbefore described with reference to the accompanying Examples. 22870041 (GHMatters) 27/05/10
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/IB2003/006105 WO2005070863A1 (en) | 2003-12-22 | 2003-12-22 | An athermal process for the concentration of garcinia extract |
Publications (2)
Publication Number | Publication Date |
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AU2003288610A1 AU2003288610A1 (en) | 2005-08-11 |
AU2003288610B2 true AU2003288610B2 (en) | 2010-06-17 |
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AU2003288610A Ceased AU2003288610B2 (en) | 2003-12-22 | 2003-12-22 | An athermal process for the concentration of Garcinia extract |
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JP (1) | JP4499664B2 (en) |
AU (1) | AU2003288610B2 (en) |
WO (1) | WO2005070863A1 (en) |
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CN109107223B (en) * | 2018-10-15 | 2021-04-13 | 潘仲巍 | Device and method for enriching ferulic acid from angelica sinensis |
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US5536516A (en) * | 1994-08-24 | 1996-07-16 | Renaissance Herbs, Inc. | Hydroxycitric acid concentrate and food products prepared therefrom |
WO2000015051A1 (en) * | 1998-09-14 | 2000-03-23 | Interhealth Nutraceuticals, Inc. | Hydroxycitric acid compositions |
-
2003
- 2003-12-22 AU AU2003288610A patent/AU2003288610B2/en not_active Ceased
- 2003-12-22 WO PCT/IB2003/006105 patent/WO2005070863A1/en active Application Filing
- 2003-12-22 JP JP2005514334A patent/JP4499664B2/en not_active Expired - Fee Related
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Publication number | Publication date |
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WO2005070863A1 (en) | 2005-08-04 |
JP2007527359A (en) | 2007-09-27 |
AU2003288610A1 (en) | 2005-08-11 |
JP4499664B2 (en) | 2010-07-07 |
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