CN106188221A - Tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof - Google Patents
Tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof Download PDFInfo
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- CN106188221A CN106188221A CN201610780808.5A CN201610780808A CN106188221A CN 106188221 A CN106188221 A CN 106188221A CN 201610780808 A CN201610780808 A CN 201610780808A CN 106188221 A CN106188221 A CN 106188221A
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- soluble plant
- tiger ear
- plant activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
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- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of tiger ear Jinsui River soluble plant activity selenium polypeptide extract, it takes following extraction step to obtain: by broken for tiger ear bronze, water raises Aqueous extracts, strainer filtering Aqueous extracts, remove residue, obtain filtrate, by filtrate with filter membrane pressure filtration, obtain micro-filtrate, by micro-filtrate with 1000 ~ 3000 dalton's ultrafilter membrane pressure filtrations, obtain ultrafiltrate, ultrafiltrate removes inorganic salt and water with 150 dalton's NF membrane pressure filtrations, obtain nanofiltration concentrated solution, nanofiltration concentrated solution is added ethanol, stand, filter, obtain precipitate, it is tiger ear Jinsui River soluble plant activity selenium polypeptide extract.The present invention uses microfiltration successively, ultrafiltration and nanofiltration, effectively removes the macromole impurity such as pectin, many sugar and starches, obtain the higher molecular weight of purity at 150 ~ 3000 daltonian tiger ear Jinsui River soluble plant activity selenium polypeptide, the method is pure physics natural process, is suitable for large-scale production and extracts tiger ear Jinsui River soluble plant activity selenium polypeptide.
Description
Technical field
The present invention relates to a kind of tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof.
Background technology
Selenium plays an important role in health.Selenium protein has that toxicity is low, bioavailability advantages of higher.Wherein
Animal selenium protein is better than Selenium in Plants albumen, and Selenium in Plants albumen is better than microorganism selenium protein, and microorganism selenium protein is better than inorganic selenium.Selenium
Yeast main component is selenomethionine, and plant organic selenium is more preferable than selenocysteine effect.Particularly molecular weight section exists
" polypeptide " of 3000 ~ 1000 and the molecular weight section pure natural small molecule active selenium polypeptide between 1000 ~ 180, digestive system can
Directly to absorb, the common selenium protein bioavailability bigger than conventional molecular weight is higher, is the forward position studied both at home and abroad of organic selenium
One of.Understanding according to inventor, so far, the development and utilization of pure natural small molecule active selenium polypeptide yet there are no report.
Ankang Prefecture is that domestic rare Se content is moderate, one of region that selenium resource distribution is the widest, and more than 50% can farming
Soil reaches middle selenium level above, and the soil that selenium content enriches is that in plant rich in selenium, the development and utilization of pure natural plant selenium protein carries
Supply good condition.
Tiger ear gold (Cardam ine hupingshanensis), is the crucifer novel species with superpower selenium rich ability.Tiger ear gold can be from
Enriching Selenium be converted into the organic selenium of seleno-amino acids form in soil and surrounding.About the extraction of this Selenium in Plants albumen,
Being presently required through defat, enzyme hydrolysis, removing glue and multi-section precipitate with ethanol step, operate complicated, loss is big, and the molecular weight of products therefrom
Relatively big, yet there are no in tiger ear gold water-soluble low molecular weight activity selenium polypeptide and method for extraction and purification thereof and preparation.
Summary of the invention
It is an object of the invention to provide a kind of tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof, suitable
Close extensive extraction tiger ear gold water-soluble low molecular weight vegetable active selenium polypeptide.
It is as follows that the present invention realizes process:
A kind of tiger ear Jinsui River soluble plant activity selenium polypeptide extract, takes following extraction step to obtain:
(1) by broken for tiger ear bronze, 30 ~ 60 DEG C of water raise Aqueous extracts；
(2) strainer filtering Aqueous extracts, removes residue, obtains filtrate；
(3) by filtrate with 0.2 m filter membrane pressure filtration, micro-filtrate is obtained；
(4) by micro-filtrate with 1000 ~ 3000 dalton's ultrafilter membrane pressure filtrations, ultrafiltrate is obtained；
(5) ultrafiltrate removes inorganic salt and water with 150 dalton's NF membrane pressure filtrations, obtains nanofiltration concentrated solution；
(6) nanofiltration concentrated solution is added ethanol, to alcohol content 60 ~ 75%, stand, filter, it is thus achieved that precipitate；
(7) dry sediment, is tiger ear Jinsui River soluble plant activity selenium polypeptide extract.
Soluble plant activity selenium polypeptide extract interpolation edible adjuvant in tiger ear Jinsui River is prepared water-soluble plant activity selenium many
Peptide supplement.
In said extracted step (1), tiger ear bronze is broken into 20 ~ 120 mesh, takes ultrasonic water to carry.
When said extracted step (1) is extracted, tiger ear gold is 1:8 ~ 1:12 with the mass ratio of water.
In said extracted step (5), when before film, liquid stops concentrating when starting muddiness and have Precipitation.
Advantages of the present invention and good effect:
(1), when water of the present invention carries, the selenium protein of the enzyme hydrolysis macromolecule that plant self exists, removable inorganic selenium are utilized；
(2) extraction of protein typically uses water to carry, and concentrates and purifies, and long high temperature concentrates or is dried and easily makes albumen qualitative change
Property；Protein precipitation uses regulation isoelectric point, IP or addition solid ammonium sulfate to reach 80% saturated method and obtains, the albumen obtained
Matter molecular weight is big, and purity is low.The present invention takes membrane filtration to concentrate and purify, it is to avoid high temperature concentrates the protein denaturation caused.This
The method of invention addition 60 ~ 75% ethanol obtains water-soluble plant activity selenium polypeptide, can effectively remove residual inorganic selenium, sugar
Class and the Organic substance of other alcohol-soluble；
(3) present invention uses microfiltration, ultrafiltration and nanofiltration successively, effectively removes the macromole impurity such as pectin, many sugar and starches,
Obtaining the higher molecular weight of purity at 150 ~ 3000 daltonian tiger ear Jinsui River soluble plant activity selenium polypeptide, the method is pure thing
Reason natural process, is suitable for large-scale production and extracts tiger ear Jinsui River soluble plant activity selenium polypeptide.
Detailed description of the invention
Embodiment 1
The extracting method of tiger ear Jinsui River soluble plant activity selenium polypeptide extract, comprises the steps:
(1) by broken for tiger ear bronze；
(2) be 1:10(mass ratio by tiger ear gold (Cardam ine hupingshanensis) powder additional proportion) water, with super under the conditions of 30 ~ 60 DEG C
Sound extracts, and utilizes the selenium protein of the enzyme hydrolysis macromolecule that plant self exists, removal inorganic selenium.Ultrasonic water carries totally 3 times, every time
30 ~ 40min, merges Aqueous extracts；
(3) strainer filtering Aqueous extracts, removes residue, obtains filtrate；
(4) by brave ear gold (Cardam ine hupingshanensis) filtrate of gained, with 0.2 m filter membrane pressure filtration, micro-filtrate is obtained；
(5) micro-filtrate is with 1000 ~ 3000 dalton's ultrafilter membrane pressure filtrations, obtains ultrafiltrate；The purpose of this step is that removing is water-soluble
The macromolecular substances of property, obtaining molecular weight less than 3000 can be the active selenium polypeptide solution that human body directly absorbs；
(6) above-mentioned ultrafiltrate is with 150 dalton's NF membrane pressure filtrations, and before observing film, liquid stops when starting muddiness and have Precipitation
Only concentrate, obtain nanofiltration concentrated solution；The purpose of this step is to remove inorganic salt and water, obtains molecular weight daltonian 150 ~ 3000
Tiger ear Jinsui River soluble plant activity selenium polypeptide concentrated solution；
(7) nanofiltration concentrated solution adds ethanol, to alcohol content 60 ~ 75%, stands, filters, it is thus achieved that precipitate；The ethanol that this step uses
For dehydrated alcohol or the ethanol of 95%；The purpose of this step is that the molecular weight obtaining higher degree is 150 ~ 3000 daltonian tigers
Ear Jinsui River soluble plant activity selenium polypeptide precipitate；
(8) it is dried gained precipitate, packaging or coordinate with other adjuvants after pulverizing, it is thus achieved that different dosage form, different appearance character
Water-soluble plant activity selenium polypeptide supplement.
The present invention uses microfiltration, ultrafiltration and nanofiltration successively, effectively removes the macromole such as pectin, many sugar and starches miscellaneous
Matter, it is thus achieved that the higher molecular weight of purity is at 150 ~ 3000 daltonian tiger ear Jinsui River soluble plant activity selenium polypeptide.This technical side
Method is pure physics natural process, is suitable for large-scale production and extracts tiger ear Jinsui River soluble plant activity selenium polypeptide.The inventive method carries
The brave ear Jinsui River soluble plant activity selenium polypeptide taken has strong garlic smell, and purity is high, through 1,2,3-indantrione monohydrate experiment detection, wherein
Water-soluble plant activity selenium content of peptides reaches more than 80%.Hydrogenated thing generation-Atomic Fluorescence Spectrometry, in product, organic selenium contains
Amount reaches more than 75mg/kg, can be directly used for selenium and supplements or prepare different dosage form, the water of different appearance character from other adjuvants
Soluble plant activity selenium polypeptide supplement.
Claims (9)
1. brave ear Jinsui River soluble plant activity selenium polypeptide extract, takes following extraction step to obtain:
(1) by broken for tiger ear bronze, 30 ~ 60 DEG C of water raise Aqueous extracts；
(2) strainer filtering Aqueous extracts, removes residue, obtains filtrate；
(3) by filtrate with 0.2 m filter membrane pressure filtration, micro-filtrate is obtained；
(4) by micro-filtrate with 1000 ~ 3000 dalton's ultrafilter membrane pressure filtrations, ultrafiltrate is obtained；
(5) ultrafiltrate removes inorganic salt and water with 150 dalton's NF membrane pressure filtrations, obtains nanofiltration concentrated solution；
(6) nanofiltration concentrated solution is added ethanol, to alcohol content 60 ~ 75%, stand, filter, it is thus achieved that precipitate；
(7) dry sediment, is tiger ear Jinsui River soluble plant activity selenium polypeptide extract.
Tiger ear Jinsui River the most according to claim 1 soluble plant activity selenium polypeptide extract, it is characterised in that: extraction step
(1), in, tiger ear bronze is broken into 20 ~ 120 mesh, takes ultrasonic water to carry.
Tiger ear Jinsui River the most according to claim 1 soluble plant activity selenium polypeptide extract, it is characterised in that: extraction step
(1), when extracting, tiger ear gold is 1:8 ~ 1:12 with the mass ratio of water.
Tiger ear Jinsui River the most according to claim 1 soluble plant activity selenium polypeptide extract, it is characterised in that: extraction step
(5) in, when before film, liquid stops concentrating when starting muddiness and have Precipitation.
5. an extracting method for brave ear Jinsui River soluble plant activity selenium polypeptide extract, comprises the following steps:
(1) by broken for tiger ear bronze, 30 ~ 60 DEG C of water raise Aqueous extracts；
(2) strainer filtering Aqueous extracts, removes residue, obtains filtrate；
(3) by filtrate with 0.2 m filter membrane pressure filtration, micro-filtrate is obtained；
(4) by micro-filtrate with 1000 ~ 3000 dalton's ultrafilter membrane pressure filtrations, ultrafiltrate is obtained；
(5) ultrafiltrate removes inorganic salt and water with 150 dalton's NF membrane pressure filtrations, obtains nanofiltration concentrated solution；
(6) nanofiltration concentrated solution is added ethanol, to alcohol content 60 ~ 75%, stand, filter, it is thus achieved that precipitate；
(7) dry sediment, is tiger ear Jinsui River soluble plant activity selenium polypeptide extract.
The extracting method of tiger ear Jinsui River soluble plant activity selenium polypeptide extract the most according to claim 5, it is characterised in that:
In step (1), tiger ear bronze is broken into 20 ~ 120 mesh, takes ultrasonic water to carry.
The extracting method of tiger ear Jinsui River soluble plant activity selenium polypeptide extract the most according to claim 5, it is characterised in that:
When step (1) is extracted, tiger ear gold is 1:8 ~ 1:12 with the mass ratio of water.
The extracting method of tiger ear Jinsui River soluble plant activity selenium polypeptide extract the most according to claim 5, it is characterised in that:
In step (5), when before film, liquid stops concentrating when starting muddiness and have Precipitation.
9. contain the water solublity that brave ear Jinsui River soluble plant activity selenium polypeptide extract and edible adjuvant described in claim 1 prepare
Vegetable active selenium polypeptide supplement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610780808.5A CN106188221A (en) | 2016-08-31 | 2016-08-31 | Tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610780808.5A CN106188221A (en) | 2016-08-31 | 2016-08-31 | Tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof |
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| CN106188221A true CN106188221A (en) | 2016-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610780808.5A Pending CN106188221A (en) | 2016-08-31 | 2016-08-31 | Tiger ear Jinsui River soluble plant activity selenium polypeptide extract and extracting method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110559352A (en) * | 2019-10-23 | 2019-12-13 | 河北科技大学 | hawthorn flavone microcapsule and preparation method and application thereof |
| CN110973637A (en) * | 2019-10-30 | 2020-04-10 | 安徽中谷生物科技有限公司 | Preparation method of plant micromolecular organic selenium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391315A (en) * | 2011-08-10 | 2012-03-28 | 恩施盛硒生物科技有限公司 | Method for extracting purely natural plant organic selenium |
| CN103588870A (en) * | 2013-11-22 | 2014-02-19 | 白宏锋 | Extraction method of cardamine hupingshanesis plant selenoprotein |
| CN104231038A (en) * | 2014-08-25 | 2014-12-24 | 华中农业大学 | Extraction and purification method of decolorized cardamine hupingshanesis selenium-containing proteins |
-
2016
- 2016-08-31 CN CN201610780808.5A patent/CN106188221A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391315A (en) * | 2011-08-10 | 2012-03-28 | 恩施盛硒生物科技有限公司 | Method for extracting purely natural plant organic selenium |
| CN103588870A (en) * | 2013-11-22 | 2014-02-19 | 白宏锋 | Extraction method of cardamine hupingshanesis plant selenoprotein |
| CN104231038A (en) * | 2014-08-25 | 2014-12-24 | 华中农业大学 | Extraction and purification method of decolorized cardamine hupingshanesis selenium-containing proteins |
Non-Patent Citations (1)
| Title |
|---|
| 蔺爱国等编著: "《新型功能膜技术及其应用》", 30 November 2013, 中国石油大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110559352A (en) * | 2019-10-23 | 2019-12-13 | 河北科技大学 | hawthorn flavone microcapsule and preparation method and application thereof |
| CN110973637A (en) * | 2019-10-30 | 2020-04-10 | 安徽中谷生物科技有限公司 | Preparation method of plant micromolecular organic selenium |
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Application publication date: 20161207 |